FvH4_5g14680

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002194
Physical Location & Seq
Reverse (-)
8336076 .. 8341329
5254 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14680.t2

Sequence Viewer

Length: 579 bp
ATGGGGAGGAGTGGATTGTTTGATCTTGAAAACCACTTTGCCTTCTATGGCGCATATCACAGCAACCCTGTTAACATTTTGATCCACACTCTGTTTGTGTGGCCTATTCTCTTCACCTCTCTTGTTCTTCTCTACTTCACACCTTCCCTGTTCAGTTTCCCAGCTGGGTTTTATCTCAATTTCGGGTTCGTGTTCACTGTGATCTATGCTTTGTATTATGTTGGATTGGATAAAAAAGCTGGGTCCTTGGCTGCTTTGCTCTGTGTTCTCTGCTGGGTTGGAGCCAGTGTTCTTGCTAATCGATTTGGGTTTTCATTGGCGTGGAAGGTTGTTTTGGCAGCTCAGTTGTTCTGTTGGGCTGGACAGTTCATAGGCCACGGGGTGTTTGAGAAACGAGCACCGGCTCTTCTGGATAACCTCGTCCAAGCATTTCTGATGGCTCCTTTCTTTGTGTTGCTTGAGGTCCTTCAAACGGCCTTTGGTTACGAACCTTATCCAGGGTTTCACTCAAGCGTGAAACAAAAGATAGAGGCCAATATCAAAGAGTGGCAGGAAGAGAAACAAAAGAAACAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

193

Amino Acids

21.7

Weight (kDa)

8.4

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 156 1.2e-24 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 76
AdeI CACNNNGTG 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 472, 497
AgsI TTSAA 2 cut(s) 29, 470
AjnI CCWGG 1 cut(s) 496
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 3 cut(s) 164, 239, 341
AluI AGCT 3 cut(s) 164, 239, 341
Alw21I GWGCWC 1 cut(s) 400
AlwI GGATC 1 cut(s) 76
AoxI GGCC 4 cut(s) 101, 373, 474, 531
ApeKI GCWGC 2 cut(s) 251, 338
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 243, 463
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 243, 463
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 238, 350
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 498
BisI GCNGC 2 cut(s) 252, 339
BlsI GCNGC 2 cut(s) 253, 340
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 2 cut(s) 243, 463
BmgT120I GGNCC 2 cut(s) 243, 463
BmiI GGNNCC 3 cut(s) 244, 283, 441
BmrFI CCNGG 1 cut(s) 498
BpuEI CTTGAG 2 cut(s) 479, 493
Bsa29I ATCGAT 1 cut(s) 301
BsaJI CCNNGG 3 cut(s) 246, 376, 497
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 497
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 498
BseCI ATCGAT 1 cut(s) 301
BseDI CCNNGG 3 cut(s) 246, 376, 497
BseLI CCNNNNNNNGG 2 cut(s) 472, 497
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 238, 350
BseYI CCCAGC 4 cut(s) 160, 164, 239, 273
BshFI GGCC 4 cut(s) 103, 375, 476, 533
BshVI ATCGAT 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 472, 497
BsnI GGCC 4 cut(s) 103, 375, 476, 533
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 22, 81, 201
BspANI GGCC 4 cut(s) 103, 375, 476, 533
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 301
BspLI GGNNCC 3 cut(s) 244, 283, 441
BspPI GGATC 1 cut(s) 76
BspQI GCTCTTC 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 3 cut(s) 246, 376, 497
BssMI GATC 3 cut(s) 22, 81, 201
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 199, 366
Bst6I CTCTTC 3 cut(s) 116, 411, 549
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 376
BstENI CCTNNNNNAGG 1 cut(s) 495
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 3 cut(s) 25, 84, 204
BstMBI GATC 3 cut(s) 22, 81, 201
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 238, 350
Bsu15I ATCGAT 1 cut(s) 301
BsuRI GGCC 4 cut(s) 103, 375, 476, 533
BsuTUI ATCGAT 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 195, 292
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 243, 463
ClaI ATCGAT 1 cut(s) 301
DdeI CTNAG 1 cut(s) 342
DpnI GATC 3 cut(s) 24, 83, 203
DpnII GATC 3 cut(s) 22, 81, 201
DraIII CACNNNGTG 1 cut(s) 382
Eam1104I CTCTTC 3 cut(s) 116, 411, 549
EarI CTCTTC 3 cut(s) 116, 411, 549
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 2 cut(s) 243, 463
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 243, 463
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaiI YATR 5 cut(s) 48, 55, 207, 219, 371
Fnu4HI GCNGC 2 cut(s) 252, 339
Fsp4HI GCNGC 2 cut(s) 252, 339
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 252, 339
GsaI CCCAGC 4 cut(s) 164, 168, 243, 277
HaeIII GGCC 4 cut(s) 103, 375, 476, 533
HapII CCGG 1 cut(s) 401
HhaI GCGC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 73, 195
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 2 cut(s) 26, 410
Hpy8I GTNNAC 2 cut(s) 73, 195
HpyAV CCTTC 4 cut(s) 52, 153, 319, 476
HpyCH4III ACNGT 2 cut(s) 199, 366
HpyF3I CTNAG 1 cut(s) 342
HspAI GCGC 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 3 cut(s) 22, 81, 201
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 2 cut(s) 281, 445
Lsp1109I GCAGC 2 cut(s) 238, 350
MaeIII GTNAC 1 cut(s) 482
MalI GATC 3 cut(s) 24, 83, 203
MboI GATC 3 cut(s) 22, 81, 201
MboII GAAGA 4 cut(s) 103, 119, 398, 566
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 1 cut(s) 178
MmeI TCCRAC 2 cut(s) 202, 259
MnlI CCTC 4 cut(s) 127, 428, 454, 523
MseI TTAA 2 cut(s) 72, 577
MslI CAYNNNNRTG 1 cut(s) 319
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
NdeII GATC 3 cut(s) 22, 81, 201
NlaIV GGNNCC 3 cut(s) 244, 283, 441
PciSI GCTCTTC 1 cut(s) 411
PkrI GCNGC 2 cut(s) 253, 340
PpuMI RGGWCCY 2 cut(s) 243, 463
Psp5II RGGWCCY 2 cut(s) 243, 463
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 4 cut(s) 160, 164, 239, 273
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 244, 283, 441
PspPI GGNCC 2 cut(s) 243, 463
PspPPI RGGWCCY 2 cut(s) 243, 463
PvuII CAGCTG 1 cut(s) 164
RseI CAYNNNNRTG 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 2 cut(s) 72, 577
SatI GCNGC 2 cut(s) 252, 339
Sau3AI GATC 3 cut(s) 22, 81, 201
Sau96I GGNCC 2 cut(s) 243, 463
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 400
SetI ASST 9 cut(s) 119, 145, 166, 241, 330, 343, 420, 465, 493
SinI GGWCC 2 cut(s) 243, 463
SmiMI CAYNNNNRTG 1 cut(s) 319
SmlI CTYRAG 2 cut(s) 458, 508
SmoI CTYRAG 2 cut(s) 458, 508
Sse9I AATT 1 cut(s) 178
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 199, 366
TaqI TCGA 1 cut(s) 301
TasI AATT 1 cut(s) 178
Tru1I TTAA 2 cut(s) 72, 577
Tru9I TTAA 2 cut(s) 72, 577
TscAI CASTG 2 cut(s) 202, 292
TseI GCWGC 2 cut(s) 251, 338
TspDTI ATGAA 2 cut(s) 303, 358
TspRI CASTG 2 cut(s) 202, 292
VpaK11BI GGWCC 2 cut(s) 243, 463
XagI CCTNNNNNAGG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.