MD14G1202900.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002194
Physical Location & Seq
Forward (+)
29115404 .. 29117422
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1202900.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGGGAGGACTGGATTGTTCGATCTGGAAAGTCACTTCGCCTTCTATGGCGCTTACCACAGCAACCCAGTTAACATTTTGATCCACACTCTGTTTGTGTGGCCGATTTTCTTCACCTCTCTGGTTCTCTTCTACTTCACACCTTCTCTCTACAATTTCCCGAACGGTTTCTGTTTGAATTTAGGGTTTGCTTTCACTGTGATCTATGCTGGGTTTTATGTGTGCTTGGATAAGAAAGCTGGGTCTGTTGCTGCTCTGCTCTGTGTTCTCTGCTGGGTTCAATCCACCCTGCTTGCTAGCCGTCTTGGGTTTTCTGTGTCATGGAAGGTTGTGTTGGCTGCTCAGTTGTTCTGTTGGACTGGACAGTTCATAGGCCATGGGGTGTTCGAGAAACGAGCACCAGCTCTTTTGGACAACCTTGTTCAAGCATTTCTAATGGCTCCTTTCTTTGTGCTGCTTGAGTGTCTTCAATCGGTATTCGGCTACGAACCATATCCAGGGTTTCATGCAAGTGTGGAAGAAAAGGTAGAAGCTCAGATCAAGGAGTGGAGGGACAAGAAACAAAAGAAAATCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

193

Amino Acids

21.77

Weight (kDa)

8.2

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 157 4.8e-24 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 4 cut(s) 178, 281, 425, 470
AjnI CCWGG 1 cut(s) 496
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 239, 404, 533
AluI AGCT 3 cut(s) 239, 404, 533
Alw21I GWGCWC 1 cut(s) 400
AlwI GGATC 1 cut(s) 76
AoxI GGCC 2 cut(s) 101, 373
ApeKI GCWGC 3 cut(s) 251, 338, 454
ApoI RAATTY 1 cut(s) 178
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 106
AsuNHI GCTAGC 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 3 cut(s) 238, 325, 441
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 498
BfaI CTAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 3 cut(s) 252, 339, 455
BlsI GCNGC 3 cut(s) 253, 340, 456
Bme1390I CCNGG 1 cut(s) 498
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 498
BmrI ACTGGG 1 cut(s) 62
BmtI GCTAGC 1 cut(s) 300
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 376, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse1I ACTGG 3 cut(s) 16, 68, 364
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 376, 497
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 2 cut(s) 356, 548
BseNI ACTGG 3 cut(s) 16, 68, 364
BseXI GCAGC 3 cut(s) 238, 325, 441
BseYI CCCAGC 3 cut(s) 209, 239, 273
BshFI GGCC 2 cut(s) 103, 375
BsiHKAI GWGCWC 1 cut(s) 400
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 2 cut(s) 103, 375
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 4 cut(s) 22, 81, 201, 537
Bsp19I CCATGG 1 cut(s) 376
BspANI GGCC 2 cut(s) 103, 375
BspCNI CTCAG 2 cut(s) 355, 547
BspLI GGNNCC 1 cut(s) 441
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 1 cut(s) 76
BsrI ACTGG 3 cut(s) 16, 68, 364
BssECI CCNNGG 2 cut(s) 376, 497
BssMI GATC 4 cut(s) 22, 81, 201, 537
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 3 cut(s) 167, 199, 366
Bst6I CTCTTC 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 294, 298
BstDEI CTNAG 3 cut(s) 342, 534, 576
BstDSI CCRYGG 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 4 cut(s) 25, 84, 204, 540
BstMBI GATC 4 cut(s) 22, 81, 201, 537
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 238, 325, 441
BstV2I GAAGAC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 103, 375
BtgI CCRYGG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 294, 298
CfoI GCGC 1 cut(s) 53
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 3 cut(s) 321, 377, 506
CviJI RGCY 9 cut(s) 103, 239, 300, 338, 375, 404, 440, 483, 533
CviKI_1 RGCY 9 cut(s) 103, 239, 300, 338, 375, 404, 440, 483, 533
DdeI CTNAG 3 cut(s) 342, 534, 576
DpnI GATC 4 cut(s) 24, 83, 203, 539
DpnII GATC 4 cut(s) 22, 81, 201, 537
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 324, 380, 509
FaiI YATR 8 cut(s) 48, 207, 219, 322, 371, 378, 493, 507
FaqI GGGAC 1 cut(s) 566
FatI CATG 3 cut(s) 320, 376, 505
Fnu4HI GCNGC 3 cut(s) 252, 339, 455
Fsp4HI GCNGC 3 cut(s) 252, 339, 455
FspBI CTAG 1 cut(s) 297
GlaI GCGC 1 cut(s) 52
GluI GCNGC 3 cut(s) 252, 339, 455
GsaI CCCAGC 3 cut(s) 213, 243, 277
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 2 cut(s) 103, 375
HhaI GCGC 1 cut(s) 53
Hin1II CATG 3 cut(s) 324, 380, 509
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 537
Hpy188III TCNNGA 3 cut(s) 26, 160, 388
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 52, 153, 319
HpyCH4III ACNGT 3 cut(s) 167, 199, 366
HpyCH4V TGCA 1 cut(s) 509
HpyF3I CTNAG 3 cut(s) 342, 534, 576
Hsp92II CATG 3 cut(s) 324, 380, 509
HspAI GCGC 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 22, 81, 201, 537
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 3 cut(s) 238, 325, 441
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 32
MalI GATC 4 cut(s) 24, 83, 203, 539
MboI GATC 4 cut(s) 22, 81, 201, 537
MboII GAAGA 4 cut(s) 103, 121, 458, 530
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 2 cut(s) 154, 178
MmeI TCCRAC 1 cut(s) 335
MnlI CCTC 2 cut(s) 127, 543
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 376
NdeII GATC 4 cut(s) 22, 81, 201, 537
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 3 cut(s) 324, 380, 509
NlaIV GGNNCC 1 cut(s) 441
NmuCI GTSAC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 253, 340, 456
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 3 cut(s) 209, 239, 273
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 441
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 3 cut(s) 252, 339, 455
Sau3AI GATC 4 cut(s) 22, 81, 201, 537
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 400
SetI ASST 8 cut(s) 119, 145, 241, 330, 406, 420, 528, 535
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 154, 178
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 3 cut(s) 167, 199, 366
TaqI TCGA 2 cut(s) 21, 387
TasI AATT 2 cut(s) 154, 178
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 3 cut(s) 251, 338, 454
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 358, 494
TspRI CASTG 1 cut(s) 202
XapI RAATTY 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.