MD06G1195800.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002194
Physical Location & Seq
Forward (+)
32981169 .. 32982893
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1195800.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGGGAGGACCGGATTGTTCGATCTGGAACGGCACTTCGCCTTCTACGGCGCTTATCACAGCAACCCAGTTAACATTTTGATCCACACACTGTTTGTGTGGCCGATTTTCTTCACCTCTCTGGTTCTCTTCCACTTCACACCTTCTCTCTACAGTTTCCCAAATGGGTTCTGTTTGAATTTTGGGTTTGCTCTCACTGCGATGTATGCTACTATGCTGGGTTTTATGTGTGCTTGGATAAGAAAGCTGGGTCTGTTGCTGCTCTGCTCTGTAGATGTACGTCTCTGGTTCGTGTGTTGGCTTCGGATGGGCTTCTTGTCCTCCTCTATAGGTCTCTCCGGTGATGGTCATGTTTGTCTAGCTCTGGTTCGGACTTTAGCTTCTCCGGTTTTCCCTGTTCTGGCGCCTTTGCCTAGCTTTGTTGTTGTGTTGGCTGCTCAGTTGTTCTGTTGGACTGGACAGTTCATAGGCCACGGGGTTTTTGAGAAACAAGCACCAGCTCTTTTGGACAACCTTGTTCAAGCGTTTCTAATCGGTCCTTTCTTTGTGCTGCTTGAGTGTCTTCAATTGGTATTCGGCTACGAACCTTATCGAGGTTTTCATGCAAGCGTGGAAGAAAAGATAGAAGCTCAGATCAAAGAGTGGCAGGACAAGAAACAAAAGAAAATCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

225

Amino Acids

25.35

Weight (kDa)

8.17

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 188 9.2e-21 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 403
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 178
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 400, 593
AgsI TTSAA 3 cut(s) 178, 521, 566
AluBI AGCT 6 cut(s) 247, 362, 380, 417, 500, 629
AluI AGCT 6 cut(s) 247, 362, 380, 417, 500, 629
Alw26I GTCTC 2 cut(s) 287, 338
AlwI GGATC 1 cut(s) 76
AoxI GGCC 2 cut(s) 101, 469
ApeKI GCWGC 3 cut(s) 259, 434, 550
ApoI RAATTY 1 cut(s) 178
AspLEI GCGC 2 cut(s) 53, 406
AspS9I GGNCC 2 cut(s) 9, 536
AsuHPI GGTGA 2 cut(s) 106, 353
AvaII GGWCC 2 cut(s) 9, 536
BanI GGYRCC 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 3 cut(s) 246, 421, 537
BccI CCATC 2 cut(s) 301, 338
BceAI ACGGC 2 cut(s) 47, 64
BcoDI GTCTC 2 cut(s) 287, 338
BfaI CTAG 2 cut(s) 359, 414
BfmI CTRYAG 3 cut(s) 151, 270, 327
BfoI RGCGCY 2 cut(s) 54, 407
BisI GCNGC 3 cut(s) 260, 435, 551
BlsI GCNGC 3 cut(s) 261, 436, 552
Bme18I GGWCC 2 cut(s) 9, 536
BmgT120I GGNCC 2 cut(s) 9, 536
BmiI GGNNCC 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 472
BsaWI WCCGGW 3 cut(s) 11, 338, 385
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 593
Bse1I ACTGG 2 cut(s) 68, 460
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 400, 593
BseMII CTCAG 2 cut(s) 452, 644
BseNI ACTGG 2 cut(s) 68, 460
BseRI GAGGAG 1 cut(s) 313
BseXI GCAGC 3 cut(s) 246, 421, 537
BseYI CCCAGC 2 cut(s) 217, 247
BshFI GGCC 2 cut(s) 103, 471
BshNI GGYRCC 1 cut(s) 403
BsiSI CCGG 3 cut(s) 12, 339, 386
BslI CCNNNNNNNGG 2 cut(s) 400, 593
BsmAI GTCTC 2 cut(s) 287, 338
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 2 cut(s) 103, 471
Bso31I GGTCTC 1 cut(s) 338
Bsp143I GATC 3 cut(s) 22, 81, 633
BspANI GGCC 2 cut(s) 103, 471
BspCNI CTCAG 2 cut(s) 451, 643
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 403
BspTNI GGTCTC 1 cut(s) 338
BsrI ACTGG 2 cut(s) 68, 460
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 3 cut(s) 22, 81, 633
BssNI GRCGYC 1 cut(s) 404
Bst4CI ACNGT 3 cut(s) 93, 155, 462
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 607
BstDEI CTNAG 3 cut(s) 438, 630, 672
BstDSI CCRYGG 1 cut(s) 472
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 1 cut(s) 312
BstH2I RGCGCY 2 cut(s) 54, 407
BstHHI GCGC 2 cut(s) 53, 406
BstKTI GATC 3 cut(s) 25, 84, 636
BstMAI GTCTC 2 cut(s) 287, 338
BstMBI GATC 3 cut(s) 22, 81, 633
BstMWI GCNNNNNNNGC 2 cut(s) 197, 206
BstSFI CTRYAG 3 cut(s) 151, 270, 327
BstV1I GCAGC 3 cut(s) 246, 421, 537
BstV2I GAAGAC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 103, 471
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 215
BtsCI GGATG 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 89, 195
Cac8I GCNNGC 1 cut(s) 607
CfoI GCGC 2 cut(s) 53, 406
Cfr13I GGNCC 2 cut(s) 9, 536
Csp6I GTAC 1 cut(s) 278
CviAII CATG 2 cut(s) 350, 602
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 3 cut(s) 438, 630, 672
DinI GGCGCC 1 cut(s) 405
DpnI GATC 3 cut(s) 24, 83, 635
DpnII GATC 3 cut(s) 22, 81, 633
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 9, 536
EcoNI CCTNNNNNAGG 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 2 cut(s) 353, 605
FaiI YATR 7 cut(s) 207, 215, 227, 329, 351, 467, 603
FatI CATG 2 cut(s) 349, 601
Fnu4HI GCNGC 3 cut(s) 260, 435, 551
FokI GGATG 1 cut(s) 319
Fsp4HI GCNGC 3 cut(s) 260, 435, 551
FspBI CTAG 2 cut(s) 359, 414
GlaI GCGC 2 cut(s) 52, 405
GluI GCNGC 3 cut(s) 260, 435, 551
GsaI CCCAGC 2 cut(s) 221, 251
HaeII RGCGCY 2 cut(s) 54, 407
HaeIII GGCC 2 cut(s) 103, 471
HapII CCGG 3 cut(s) 12, 339, 386
HhaI GCGC 2 cut(s) 53, 406
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 2 cut(s) 353, 605
Hin6I GCGC 2 cut(s) 51, 404
HinP1I GCGC 2 cut(s) 51, 404
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 3 cut(s) 12, 339, 386
HphI GGTGA 2 cut(s) 106, 353
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 306, 372, 633
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 52, 153
HpyCH4III ACNGT 3 cut(s) 93, 155, 462
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 1 cut(s) 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 206
HpyF3I CTNAG 3 cut(s) 438, 630, 672
HpySE526I ACGT 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 2 cut(s) 353, 605
HspAI GCGC 2 cut(s) 51, 404
KasI GGCGCC 1 cut(s) 403
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 3 cut(s) 22, 81, 633
Lsp1109I GCAGC 3 cut(s) 246, 421, 537
MaeI CTAG 2 cut(s) 359, 414
MaeII ACGT 1 cut(s) 280
MalI GATC 3 cut(s) 24, 83, 635
MboI GATC 3 cut(s) 22, 81, 633
MboII GAAGA 4 cut(s) 103, 121, 554, 626
MfeI CAATTG 1 cut(s) 566
MluCI AATT 2 cut(s) 178, 566
Mly113I GGCGCC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 4 cut(s) 127, 331, 334, 587
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 3 cut(s) 12, 339, 386
MunI CAATTG 1 cut(s) 566
MwoI GCNNNNNNNGC 2 cut(s) 197, 206
NarI GGCGCC 1 cut(s) 404
NdeII GATC 3 cut(s) 22, 81, 633
NlaIII CATG 2 cut(s) 353, 605
NlaIV GGNNCC 1 cut(s) 405
PkrI GCNGC 3 cut(s) 261, 436, 552
PluTI GGCGCC 1 cut(s) 407
PspFI CCCAGC 2 cut(s) 217, 247
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 2 cut(s) 9, 536
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 3 cut(s) 260, 435, 551
Sau3AI GATC 3 cut(s) 22, 81, 633
Sau96I GGNCC 2 cut(s) 9, 536
SfcI CTRYAG 3 cut(s) 151, 270, 327
SfoI GGCGCC 1 cut(s) 405
SinI GGWCC 2 cut(s) 9, 536
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 2 cut(s) 178, 566
SspDI GGCGCC 1 cut(s) 403
SspMI CTAG 2 cut(s) 359, 414
TaaI ACNGT 3 cut(s) 93, 155, 462
TaiI ACGT 1 cut(s) 283
TaqI TCGA 2 cut(s) 21, 592
TaqII GACCGA 1 cut(s) 524
TasI AATT 2 cut(s) 178, 566
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 2 cut(s) 96, 202
TseI GCWGC 3 cut(s) 259, 434, 550
TspDTI ATGAA 2 cut(s) 454, 590
TspRI CASTG 2 cut(s) 96, 202
VpaK11BI GGWCC 2 cut(s) 9, 536
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 1 cut(s) 178
XspI CTAG 2 cut(s) 359, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.