AT1G77405

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006654
Physical Location & Seq
Forward (+)
29087126 .. 29088521
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77405.1

Sequence Viewer

Length: 1377 bp
ATGAAACCGTCGCAACCGCTATGTAACCGCATTGTTGATCAGCTTATTACTGCCATGATTCAGAACCGTCCATTCGACGCCGTTCTCGCTTCTTCAACGGTGGCGAAACCATGGACTCAGCAGCTCGTCTCCGATGTCCTCCATTCTATCCCCAGATTCTTCTTTATCTCCCCACGATCCATCGGTCGGCAAAAGGGTTTTCGTCACCGTTCACCGTTAAAGCAGAGAAACCTCAGTGATGAATCACAACGACGTCGTTCTGAAGTTCTAGTTCTAGGTCCCGGTGCGTATATGGATCCTAAGAAAGTCTCTATCGGGTTGCAGAAAGCATTGGAGTTCTTCTTTTGGATCGAAACCCACTTCGGGTTTGATCATAATGAGATCACTTGCAGAGATATGGCTTGTCTATTGGCCAAAGGCAATGACTTTAAGGGTCTCTGGGATTTTCTCCGGCAAGTCTCTAGAAGAGAGAACGGTAAAAATGTGGTCACAACAGCGTCTATAACCTGTTTGATGAAATGCCTAGGAGAAGAAGGTTTTGTGAAAGAGGCATTAGCTACATTTTATAGGATGAAGGAATATCACTGCAAACCTGATGTTTATGCTTACAATACAATTATCAATGCCCTTTGCCGTGTGGGGAATTTTAAAAAAGCTCGGTTTCTATTGGATCAGATGCAATTACCGGGTTTTCGGTACCCACCGGATACCTATACTTACACCATTTTGATTAGCTCCTACTGTAGATATGGGATGCAAACCGGATGCAGAAAGGCGATAAGGAGGAGAATGTGGGAGGCTAATCGAATGTTTAGGGAAATGCTGTTCAGAGGGTTTGTACCCGATGTGGTGACTTATAATTGCTTGATCGATGGATGTTGCAAAACGAATCGGATTGGTCGGGCTCTGGAGCTGTTTGAAGATATGAAGACAAAAGGGTGTGTACCGAACCAAGTGACTTATAATTCTTTCATAAGGTATTATAGTGTTACTAATGAGATAGAAGGTGCAATAGAGATGATGAGGACAATGAAGAAGTTGGGTCATGGTGTACCTGGTTCGAGCACGTACACGCCGCTAATACACGCTCTTGTTGAGACCAGGAGAGCGGCTGAGGCTAGGGATTTGGTGGTGGAGATGGTGGAGGCTGGGTTGGTTCCGAGAGAGTATACATATAAGTTGGTGTGTGATGCTCTGAGCTCAGAAGGACTGGCAAGCACACTTGATGAAGAGCTGCATAAGAGAATGAGGGAAGGAATACAACAGAGATATAGCAGAGTCATGAAGATCAAACCGACCATGGCTCGAAAAGAGGTTGTTCGAAAATATTTTCACAAGATTGACGGGAATCAAAATTTTGCAATGGAGGAAATCTGA

Protein Analysis

458

Amino Acids

52.99

Weight (kDa)

9.45

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 165 - 212 2e-10 PPR repeat family
PPR_1 PF12854 195 - 227 1.6e-10 PPR repeat
PPR_2 PF13041 198 - 249 2.5e-14 PPR repeat family
PPR_2 PF13041 262 - 295 1.2e-09 PPR repeat family
PPR_3 PF13812 270 - 328 5.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 277 - 310 2.3e-13 PPR repeat
PPR_2 PF13041 281 - 326 6.8e-19 PPR repeat family
PPR PF01535 284 - 314 8.5e-10 PPR repeat
PPR_long PF17177 285 - 417 6.9e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 316 - 348 8.1e-06 PPR repeat family
PPR_2 PF13041 352 - 399 7.3e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 858, 963
AatII GACGTC 1 cut(s) 256
Acc65I GGTACC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 696
AccBSI CCGCTC 1 cut(s) 1109
AccI GTMKAC 1 cut(s) 1169
AciI CCGC 4 cut(s) 17, 28, 1076, 1109
AclWI GGATC 5 cut(s) 171, 290, 303, 356, 678
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 2 cut(s) 643, 1354
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 2 cut(s) 78, 253
AfaI GTAC 5 cut(s) 698, 840, 945, 1053, 1070
AfiI CCNNNNNNNGG 2 cut(s) 186, 363
AgsI TTSAA 2 cut(s) 96, 920
AjnI CCWGG 2 cut(s) 1054, 1100
AloI GAACNNNNNNTCC 2 cut(s) 809, 841
AluBI AGCT 8 cut(s) 43, 124, 557, 656, 735, 913, 1200, 1234
AluI AGCT 8 cut(s) 43, 124, 557, 656, 735, 913, 1200, 1234
Alw21I GWGCWC 2 cut(s) 1067, 1202
Alw26I GTCTC 5 cut(s) 133, 313, 440, 463, 1091
AlwI GGATC 5 cut(s) 171, 290, 303, 356, 678
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 121, 1234
ApoI RAATTY 2 cut(s) 643, 1354
Asp718I GGTACC 1 cut(s) 696
AspA2I CCTAGG 1 cut(s) 523
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 2 cut(s) 282, 687
AsuHPI GGTGA 3 cut(s) 197, 204, 862
AsuII TTCGAA 1 cut(s) 1321
AvaII GGWCC 1 cut(s) 278
AvrII CCTAGG 1 cut(s) 523
BalI TGGCCA 1 cut(s) 413
BamHI GGATCC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 696
BanII GRGCYC 2 cut(s) 907, 1202
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 2 cut(s) 1067, 1202
BbvCI CCTCAGC 1 cut(s) 1113
BbvI GCAGC 2 cut(s) 133, 1221
BccI CCATC 3 cut(s) 188, 866, 1132
BceAI ACGGC 2 cut(s) 65, 618
BciT130I CCWGG 2 cut(s) 1056, 1102
BciVI GTATCC 1 cut(s) 700
BclI TGATCA 2 cut(s) 37, 370
BcnI CCSGG 2 cut(s) 282, 687
BcoDI GTCTC 5 cut(s) 133, 313, 440, 463, 1091
BfaI CTAG 5 cut(s) 269, 275, 462, 524, 1119
BfmI CTRYAG 1 cut(s) 742
BfuI GTATCC 1 cut(s) 700
BisI GCNGC 4 cut(s) 122, 1076, 1110, 1235
BlnI CCTAGG 1 cut(s) 523
BlsI GCNGC 4 cut(s) 123, 1077, 1111, 1236
Bme1390I CCNGG 4 cut(s) 282, 687, 1056, 1102
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 4 cut(s) 280, 297, 698, 1158
BmrFI CCNGG 4 cut(s) 282, 687, 1056, 1102
BmsI GCATC 4 cut(s) 666, 744, 755, 1180
BpiI GAAGAC 1 cut(s) 935
BpmI CTGGAG 1 cut(s) 929
Bpu10I CCTNAGC 1 cut(s) 1113
Bpu14I TTCGAA 1 cut(s) 1321
BpuMI CCSGG 2 cut(s) 282, 687
Bsa29I ATCGAT 1 cut(s) 870
BsaAI YACGTR 1 cut(s) 1068
BsaHI GRCGYC 2 cut(s) 78, 253
BsaI GGTCTC 2 cut(s) 440, 1091
BsaJI CCNNGG 3 cut(s) 110, 523, 1299
BsaWI WCCGGW 2 cut(s) 703, 761
BsaXI ACNNNNNCTCC 2 cut(s) 1136, 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 363
Bse1I ACTGG 1 cut(s) 1215
Bse3DI GCAATG 2 cut(s) 427, 1368
BseBI CCWGG 2 cut(s) 1056, 1102
BseCI ATCGAT 1 cut(s) 870
BseDI CCNNGG 3 cut(s) 110, 523, 1299
BseGI GGATG 4 cut(s) 576, 759, 770, 881
BseLI CCNNNNNNNGG 2 cut(s) 186, 363
BseMI GCAATG 2 cut(s) 427, 1368
BseMII CTCAG 5 cut(s) 131, 247, 1104, 1187, 1215
BseNI ACTGG 1 cut(s) 1215
BseRI GAGGAG 1 cut(s) 799
BseXI GCAGC 2 cut(s) 133, 1221
BseYI CCCAGC 1 cut(s) 1148
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 413
BshNI GGYRCC 1 cut(s) 696
BshVI ATCGAT 1 cut(s) 870
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 1067, 1202
BsiSI CCGG 5 cut(s) 282, 451, 686, 704, 762
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 186, 363
BsmAI GTCTC 5 cut(s) 133, 313, 440, 463, 1091
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 1 cut(s) 413
Bso31I GGTCTC 2 cut(s) 440, 1091
Bsp119I TTCGAA 1 cut(s) 1321
Bsp1286I GDGCHC 3 cut(s) 907, 1067, 1202
Bsp143I GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
Bsp19I CCATGG 2 cut(s) 110, 1299
BspACI CCGC 4 cut(s) 17, 28, 1076, 1109
BspANI GGCC 1 cut(s) 413
BspCNI CTCAG 5 cut(s) 130, 246, 1105, 1188, 1214
BspDI ATCGAT 1 cut(s) 870
BspHI TCATGA 1 cut(s) 1281
BspLI GGNNCC 4 cut(s) 280, 297, 698, 1158
BspPI GGATC 5 cut(s) 171, 290, 303, 356, 678
BspQI GCTCTTC 1 cut(s) 1224
BspT104I TTCGAA 1 cut(s) 1321
BspT107I GGYRCC 1 cut(s) 696
BspTNI GGTCTC 2 cut(s) 440, 1091
BsrBI CCGCTC 1 cut(s) 1109
BsrDI GCAATG 2 cut(s) 427, 1368
BsrI ACTGG 1 cut(s) 1215
BssECI CCNNGG 3 cut(s) 110, 523, 1299
BssMI GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
BssNAI GTATAC 1 cut(s) 1170
BssNI GRCGYC 2 cut(s) 78, 253
BssT1I CCWWGG 3 cut(s) 110, 523, 1299
Bst1107I GTATAC 1 cut(s) 1170
Bst2UI CCWGG 2 cut(s) 1056, 1102
Bst4CI ACNGT 7 cut(s) 9, 68, 100, 209, 216, 476, 743
Bst6I CTCTTC 2 cut(s) 460, 1224
BstACI GRCGYC 2 cut(s) 78, 253
BstBAI YACGTR 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 1321
BstC8I GCNNGC 1 cut(s) 1216
BstDEI CTNAG 6 cut(s) 117, 233, 300, 1113, 1196, 1201
BstDSI CCRYGG 2 cut(s) 110, 1299
BstF5I GGATG 4 cut(s) 576, 759, 770, 881
BstKTI GATC 9 cut(s) 40, 179, 298, 351, 373, 384, 673, 870, 1290
BstMAI GTCTC 5 cut(s) 133, 313, 440, 463, 1091
BstMBI GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 2 cut(s) 86, 1115
BstNI CCWGG 2 cut(s) 1056, 1102
BstSCI CCNGG 4 cut(s) 280, 685, 1054, 1100
BstSFI CTRYAG 1 cut(s) 742
BstV1I GCAGC 2 cut(s) 133, 1221
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 1 cut(s) 1170
Bsu15I ATCGAT 1 cut(s) 870
BsuI GTATCC 1 cut(s) 700
BsuRI GGCC 1 cut(s) 413
BsuTUI ATCGAT 1 cut(s) 870
BtgI CCRYGG 2 cut(s) 110, 1299
BtsCI GGATG 4 cut(s) 576, 759, 770, 881
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 2 cut(s) 241, 583
Cac8I GCNNGC 1 cut(s) 1216
CciI TCATGA 1 cut(s) 1281
Cfr13I GGNCC 1 cut(s) 278
ClaI ATCGAT 1 cut(s) 870
CseI GACGC 2 cut(s) 86, 486
CsiI ACCWGGT 1 cut(s) 1054
Csp6I GTAC 5 cut(s) 697, 839, 944, 1052, 1069
CviAII CATG 5 cut(s) 55, 111, 1046, 1282, 1300
CviQI GTAC 5 cut(s) 697, 839, 944, 1052, 1069
DdeI CTNAG 6 cut(s) 117, 233, 300, 1113, 1196, 1201
DpnI GATC 9 cut(s) 39, 178, 297, 350, 372, 383, 672, 869, 1289
DpnII GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
DraI TTTAAA 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 460, 1224
EarI CTCTTC 2 cut(s) 460, 1224
Ecl136II GAGCTC 1 cut(s) 1200
Eco130I CCWWGG 3 cut(s) 110, 523, 1299
Eco24I GRGCYC 2 cut(s) 907, 1202
Eco31I GGTCTC 2 cut(s) 440, 1091
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 1200
Eco57I CTGAAG 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 1200
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 1054, 1100
EcoT14I CCWWGG 3 cut(s) 110, 523, 1299
EcoT38I GRGCYC 2 cut(s) 907, 1202
ErhI CCWWGG 3 cut(s) 110, 523, 1299
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 5 cut(s) 58, 114, 1049, 1285, 1303
FaqI GGGAC 1 cut(s) 264
FatI CATG 5 cut(s) 54, 110, 1045, 1281, 1299
FbaI TGATCA 2 cut(s) 37, 370
FblI GTMKAC 1 cut(s) 1169
Fnu4HI GCNGC 4 cut(s) 122, 1076, 1110, 1235
FokI GGATG 4 cut(s) 583, 766, 777, 888
FriOI GRGCYC 2 cut(s) 907, 1202
Fsp4HI GCNGC 4 cut(s) 122, 1076, 1110, 1235
FspBI CTAG 5 cut(s) 269, 275, 462, 524, 1119
GluI GCNGC 4 cut(s) 122, 1076, 1110, 1235
GsaI CCCAGC 1 cut(s) 1152
GsuI CTGGAG 1 cut(s) 929
HaeIII GGCC 1 cut(s) 413
HapII CCGG 5 cut(s) 282, 451, 686, 704, 762
HgaI GACGC 2 cut(s) 86, 486
Hin1I GRCGYC 2 cut(s) 78, 253
Hin1II CATG 5 cut(s) 58, 114, 1049, 1285, 1303
HinfI GANTC 7 cut(s) 58, 115, 156, 242, 889, 1278, 1348
HpaII CCGG 5 cut(s) 282, 451, 686, 704, 762
HphI GGTGA 3 cut(s) 197, 204, 862
Hpy166II GTNNAC 5 cut(s) 212, 944, 1052, 1071, 1170
Hpy188III TCNNGA 3 cut(s) 462, 908, 1282
Hpy8I GTNNAC 5 cut(s) 212, 944, 1052, 1071, 1170
Hpy99I CGWCG 4 cut(s) 13, 80, 255, 258
HpyAV CCTTC 5 cut(s) 527, 568, 998, 1199, 1247
HpyCH4III ACNGT 7 cut(s) 9, 68, 100, 209, 216, 476, 743
HpyCH4IV ACGT 2 cut(s) 253, 1067
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 1115
HpyF3I CTNAG 6 cut(s) 117, 233, 300, 1113, 1196, 1201
HpySE526I ACGT 2 cut(s) 253, 1067
Hsp92I GRCGYC 2 cut(s) 78, 253
Hsp92II CATG 5 cut(s) 58, 114, 1049, 1285, 1303
KpnI GGTACC 1 cut(s) 700
Ksp22I TGATCA 2 cut(s) 37, 370
Kzo9I GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 2 cut(s) 740, 910
Lsp1109I GCAGC 2 cut(s) 133, 1221
LweI GCATC 4 cut(s) 666, 744, 755, 1180
MabI ACCWGGT 1 cut(s) 1054
MaeI CTAG 5 cut(s) 269, 275, 462, 524, 1119
MaeII ACGT 2 cut(s) 253, 1067
MaeIII GTNAC 6 cut(s) 23, 203, 487, 850, 955, 988
MalI GATC 9 cut(s) 39, 178, 297, 350, 372, 383, 672, 869, 1289
MbiI CCGCTC 1 cut(s) 1109
MboI GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 3 cut(s) 907, 1067, 1202
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 6 cut(s) 615, 643, 680, 859, 964, 1354
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 2 cut(s) 109, 1287
Mox20I TGGCCA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 413
MseI TTAA 3 cut(s) 218, 429, 648
Msp20I TGGCCA 1 cut(s) 413
MspI CCGG 5 cut(s) 282, 451, 686, 704, 762
MspR9I CCNGG 4 cut(s) 282, 687, 1056, 1102
MvaI CCWGG 2 cut(s) 1056, 1102
MwoI GCNNNNNNNGC 2 cut(s) 86, 1115
NciI CCSGG 2 cut(s) 282, 687
NcoI CCATGG 2 cut(s) 110, 1299
NdeII GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
NlaIII CATG 5 cut(s) 58, 114, 1049, 1285, 1303
NlaIV GGNNCC 4 cut(s) 280, 297, 698, 1158
NmuCI GTSAC 4 cut(s) 203, 487, 850, 955
NspV TTCGAA 1 cut(s) 1321
PagI TCATGA 1 cut(s) 1281
PciSI GCTCTTC 1 cut(s) 1224
PfeI GAWTC 5 cut(s) 58, 156, 242, 889, 1348
PkrI GCNGC 4 cut(s) 123, 1077, 1111, 1236
PleI GAGTC 2 cut(s) 109, 1286
PpsI GAGTC 2 cut(s) 109, 1286
Ppu21I YACGTR 1 cut(s) 1068
PpuMI RGGWCCY 1 cut(s) 278
PsiI TTATAA 2 cut(s) 858, 963
Psp124BI GAGCTC 1 cut(s) 1202
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 1054, 1100
PspFI CCCAGC 1 cut(s) 1148
PspGI CCWGG 2 cut(s) 1054, 1100
PspN4I GGNNCC 4 cut(s) 280, 297, 698, 1158
PspPI GGNCC 1 cut(s) 278
PspPPI RGGWCCY 1 cut(s) 278
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 5 cut(s) 698, 840, 945, 1053, 1070
RsaNI GTAC 5 cut(s) 697, 839, 944, 1052, 1069
SacI GAGCTC 1 cut(s) 1202
SapI GCTCTTC 1 cut(s) 1224
SaqAI TTAA 3 cut(s) 218, 429, 648
SatI GCNGC 4 cut(s) 122, 1076, 1110, 1235
Sau3AI GATC 9 cut(s) 37, 176, 295, 348, 370, 381, 670, 867, 1287
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 2 cut(s) 109, 1287
ScrFI CCNGG 4 cut(s) 282, 687, 1056, 1102
SduI GDGCHC 3 cut(s) 907, 1067, 1202
SexAI ACCWGGT 1 cut(s) 1054
SfaNI GCATC 4 cut(s) 666, 744, 755, 1180
SfcI CTRYAG 1 cut(s) 742
SfuI TTCGAA 1 cut(s) 1321
SinI GGWCC 1 cut(s) 278
Sse9I AATT 6 cut(s) 615, 643, 680, 859, 964, 1354
SsiI CCGC 4 cut(s) 17, 28, 1076, 1109
SspI AATATT 1 cut(s) 1328
SspMI CTAG 5 cut(s) 269, 275, 462, 524, 1119
SstI GAGCTC 1 cut(s) 1202
StyD4I CCNGG 4 cut(s) 280, 685, 1054, 1100
StyI CCWWGG 3 cut(s) 110, 523, 1299
TaaI ACNGT 7 cut(s) 9, 68, 100, 209, 216, 476, 743
TaiI ACGT 2 cut(s) 256, 1070
TaqI TCGA 7 cut(s) 75, 351, 805, 870, 1061, 1306, 1321
TaqII GACCGA 1 cut(s) 173
TasI AATT 6 cut(s) 615, 643, 680, 859, 964, 1354
TauI GCSGC 2 cut(s) 1078, 1112
TfiI GAWTC 5 cut(s) 58, 156, 242, 889, 1348
Tru1I TTAA 3 cut(s) 218, 429, 648
Tru9I TTAA 3 cut(s) 218, 429, 648
TscAI CASTG 2 cut(s) 241, 590
TseFI GTSAC 4 cut(s) 203, 487, 850, 955
TseI GCWGC 2 cut(s) 121, 1234
Tsp45I GTSAC 4 cut(s) 203, 487, 850, 955
TspDTI ATGAA 9 cut(s) 17, 255, 530, 587, 941, 961, 1046, 1242, 1298
TspRI CASTG 2 cut(s) 241, 590
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 2 cut(s) 643, 1354
XbaI TCTAGA 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 1169
XspI CTAG 5 cut(s) 269, 275, 462, 524, 1119
ZraI GACGTC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.