RLG00000011697

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006654
Physical Location & Seq
Forward (+)
11810871 .. 11811838
968 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011697

Sequence Viewer

Length: 612 bp
ATGGCTATCGTTTTGGCTAAAGCGAGTCTGTTGAAGGCCCTCTGGGATTTTCTCAAAGACATGTCAAACTGCCAAAGAGAGGGAGAGGTGAAAGCCCTCTTGTTACGACACAGTCCATCACCTTTTATGAAATGCCTAGGAGAAGACGGCTTGGTTAATGAAGCATTGGCGGCTTTCTATAGAATGAAAATAGAGAATTTTAAGAAGGCCCGGTTTTTGCTGGAGCAGGTGGAGCTACTGGGGTTAAGATGCCCGCCGGATACATTTAGTTGTACGATTTTGATAAGCTCTTATTGTAGATATGGAGGGTTTGTTCCTGATGTTGTCACTTACAATGTTTTGATCAATGGTTGTTGCAAGACTTACAGGATAGAGAGGGTGTTGGAGTTGTTTGAGGATATGAAGAAGAGGGATTGTACCCCCATCCGGGTTACTTATGATTCATTTATTAGATACTATGCTGCTGTTAATGAGATTGATAAGGCTGTTGATATGTTAAGAAGGATGCATAGGCTGAATCATGGATTAGCCACTTCGAGTTCATATACCCCAATCATTCATGCCTTGTGTGAGGCAGGAAGGGTTGCAGAAGCCCGAAATTTTCTTGTTTGA

Protein Analysis

204

Amino Acids

23.2

Weight (kDa)

8.7

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 102 - 153 9.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 103 - 135 3.4e-09 PPR repeat
PPR_2 PF13041 106 - 146 4.1e-13 PPR repeat family
PPR PF01535 109 - 139 4e-09 PPR repeat
PPR_long PF17177 110 - 198 2.9e-07 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
AciI CCGC 2 cut(s) 170, 254
AcsI RAATTY 2 cut(s) 196, 598
AfaI GTAC 2 cut(s) 274, 418
AfiI CCNNNNNNNGG 3 cut(s) 79, 426, 427
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
AluBI AGCT 2 cut(s) 235, 288
AluI AGCT 2 cut(s) 235, 288
AoxI GGCC 2 cut(s) 36, 207
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 196, 598
AspA2I CCTAGG 1 cut(s) 136
AspS9I GGNCC 2 cut(s) 37, 208
AsuC2I CCSGG 2 cut(s) 211, 428
AsuHPI GGTGA 2 cut(s) 100, 111
AvrII CCTAGG 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 448
BccI CCATC 2 cut(s) 124, 431
BceAI ACGGC 1 cut(s) 163
BciVI GTATCC 1 cut(s) 253
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 2 cut(s) 211, 428
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 217
BfuI GTATCC 1 cut(s) 253
BisI GCNGC 2 cut(s) 171, 462
BlnI CCTAGG 1 cut(s) 136
BlsI GCNGC 2 cut(s) 172, 463
Bme1390I CCNGG 2 cut(s) 211, 428
BmgT120I GGNCC 2 cut(s) 37, 208
BmrFI CCNGG 2 cut(s) 211, 428
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 239, 495
BmuI ACTGGG 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 211, 428
BsaJI CCNNGG 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 426, 427
Bse1I ACTGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 2 cut(s) 423, 510
BseLI CCNNNNNNNGG 3 cut(s) 79, 426, 427
BseNI ACTGG 1 cut(s) 243
BseXI GCAGC 1 cut(s) 448
BshFI GGCC 2 cut(s) 38, 209
BsiSI CCGG 3 cut(s) 211, 257, 427
BslI CCNNNNNNNGG 3 cut(s) 79, 426, 427
BsnI GGCC 2 cut(s) 38, 209
Bsp143I GATC 1 cut(s) 342
BspACI CCGC 2 cut(s) 170, 254
BspANI GGCC 2 cut(s) 38, 209
BspMI ACCTGC 1 cut(s) 217
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 113
Bst6I CTCTTC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 254
BstF5I GGATG 2 cut(s) 423, 510
BstKTI GATC 1 cut(s) 345
BstMBI GATC 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 170, 232
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 2 cut(s) 209, 426
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 448
BstV2I GAAGAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 2 cut(s) 38, 209
BtsCI GGATG 2 cut(s) 423, 510
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 37, 208
Csp6I GTAC 2 cut(s) 273, 417
CviAII CATG 3 cut(s) 61, 521, 560
CviQI GTAC 2 cut(s) 273, 417
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 3 cut(s) 64, 524, 563
FatI CATG 3 cut(s) 60, 520, 559
FauI CCCGC 1 cut(s) 261
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 171, 462
FokI GGATG 2 cut(s) 410, 517
Fsp4HI GCNGC 2 cut(s) 171, 462
FspBI CTAG 1 cut(s) 137
GluI GCNGC 2 cut(s) 171, 462
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 38, 209
HapII CCGG 3 cut(s) 211, 257, 427
Hin1II CATG 3 cut(s) 64, 524, 563
HinfI GANTC 3 cut(s) 25, 440, 517
HpaII CCGG 3 cut(s) 211, 257, 427
HphI GGTGA 2 cut(s) 100, 111
Hpy188III TCNNGA 1 cut(s) 317
HpyAV CCTTC 4 cut(s) 28, 199, 495, 573
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 3 cut(s) 357, 508, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 232
Hsp92II CATG 3 cut(s) 64, 524, 563
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 223, 232
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 2 cut(s) 239, 495
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 3 cut(s) 102, 325, 430
MalI GATC 1 cut(s) 344
MboI GATC 1 cut(s) 342
MboII GAAGA 3 cut(s) 155, 415, 418
MluCI AATT 2 cut(s) 196, 598
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 9 cut(s) 50, 73, 79, 107, 299, 369, 388, 402, 565
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 5 cut(s) 156, 201, 245, 468, 497
MspI CCGG 3 cut(s) 211, 257, 427
MspR9I CCNGG 2 cut(s) 211, 428
MwoI GCNNNNNNNGC 2 cut(s) 170, 232
NciI CCSGG 2 cut(s) 211, 428
NdeII GATC 1 cut(s) 342
NlaIII CATG 3 cut(s) 64, 524, 563
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 510
NspI RCATGY 1 cut(s) 64
PaqCI CACCTGC 1 cut(s) 217
PciI ACATGT 1 cut(s) 60
PfeI GAWTC 2 cut(s) 440, 517
PflFI GACNNNGTC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 172, 463
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PscI ACATGT 1 cut(s) 60
PspPI GGNCC 2 cut(s) 37, 208
PsyI GACNNNGTC 1 cut(s) 111
RsaI GTAC 2 cut(s) 274, 418
RsaNI GTAC 2 cut(s) 273, 417
SaqAI TTAA 5 cut(s) 156, 201, 245, 468, 497
SatI GCNGC 2 cut(s) 171, 462
Sau3AI GATC 1 cut(s) 342
Sau96I GGNCC 2 cut(s) 37, 208
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 211, 428
SetI ASST 5 cut(s) 90, 124, 231, 237, 290
SfaNI GCATC 2 cut(s) 239, 495
SfcI CTRYAG 1 cut(s) 178
Sse9I AATT 2 cut(s) 196, 598
SsiI CCGC 2 cut(s) 170, 254
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 209, 426
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 536
TasI AATT 2 cut(s) 196, 598
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 440, 517
Tru1I TTAA 5 cut(s) 156, 201, 245, 468, 497
Tru9I TTAA 5 cut(s) 156, 201, 245, 468, 497
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 7 cut(s) 143, 174, 200, 416, 432, 531, 548
Tth111I GACNNNGTC 1 cut(s) 111
XapI RAATTY 2 cut(s) 196, 598
XceI RCATGY 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 136
XspI CTAG 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.