Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006654

Physical Location & Seq

Genomic Coordinates Reverse (-)

4910906 .. 4913743

Gene Structure 2838 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1030400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGAACGGTTCAAAACCTTTCAAACCCATAGTCAACCAAGTCCTCGCCGCCATGCTCAAAAACCAGCCCTTCGATTCCAATCTCGCCGCCTCCGCCGCCGCCTCCGCCGCCGCCTCCGCCGCCGCCTCTCAGCCGTGGACTGCGGACTCGGTTTCCCAAGTCCTAATTTCCATACCCAGATTCTTCTTCCAGTCCCCCCGCTCCGTCGGCCGCCAAACCGGGTTCCGCCACCGCGCTCCATTGAAGCAACGCAACCTCAGGCAAGAATCTTACAAATTCCACAACAACGTGCTCGTTCTCGGCCCGGCAGCCCACAGAGATCTCGACAAAGTTCAGCTCGGGTCGGACAAAGCACTCGAATTCTACTACTGGGTCGAAACCCGTTTCAGGTTTGCCCACAACGAACGGACTTGCAGGGAGATGGCGATTGTTTTGGCCAGAGGGAATAGGTTGAAAGCCCTTTGGGATTTTCTCAAAGAAATGTCGAAGAGAGGGAGCGGCGGGCTTGTTACCACAGAGAGCGTTACCTGTTTGATAAAAGTCCTAGGAGAGGAAGGCTTGGTTAATGATGCATTGGCTGCTTTTTATAGAATGAAGCAGTTTCATTGCAAGCCGGATGTGTATGCTTATAACACCATTATTTATGCTCTTTGCAGAGTTGGGAATTTCAATAAGGCCCGGTTTTTGTTGGAGCAGATGGAGTTGCCGGGGTTTAGATGCCCGCCGGATGTGTTCACTTATACGATTTTGATTAGTTCTTATTGTAGGTATGGTTTAGAGACGGGGTGTAGGAAGGCCATCCGGAGGCGGTTGTGGGAAGCCAACCACATGTTTCGAAACATGCTTTTCAGTGGTTTTGTTCCTGATGTTGTCACTTACAATTCTTTGATCAATGGTTGTTGCAAAACTTACCGGATTGAGAGGGCGTTGGAGTTGTTTGAAGATATGAACAAAAGGGGTTGTACCCCCAATCGGGTTACTTATGATTCGTTTATTAGGTACTATGCTGCAGTTAATGAGATTGATAAGGCTGTTGAAATGCTATGGAAGATGCAAAATTTGAAACACGGAATGCCTACTTCAAGTTCGTATACTCCAATCATTCATGCCTTGTGTGAAGCAGGAAGGATCATAGATGCCCGAGATTTTCTTGTTGAGATGATTGATGGAGGCTCGATACCTAGAGAGTATACGTATAAATTAGTTTGTTATGCACTAAATTCAGCTGGAGAAGTCAATTTGCTTGACGATGATGTGCGTAAAACAATAAAAGACGGTATAGTGAGCAGGTATAGGCAGACAATGAAGGTGAAGCCAATGATGAGTCGCAAAGGATATGATAGCATGGTGGAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

453

Amino Acids

51.59

Weight (kDa)

9.4

Isoelectric Point (pI)

33.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	174 - 219	5.4e-09	PPR repeat family
PPR_1	PF12854	202 - 234	1.6e-09	PPR repeat
PPR_2	PF13041	205 - 256	2.6e-13	PPR repeat family
PPR_1	PF12854	240 - 258	2e-06	PPR repeat
PPR_2	PF13041	271 - 302	4.9e-08	PPR repeat family
PPR_3	PF13812	277 - 335	1.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	285 - 316	4.7e-12	PPR repeat
PPR_2	PF13041	288 - 334	6.8e-18	PPR repeat family
PPR	PF01535	291 - 321	1e-09	PPR repeat
PPR_long	PF17177	292 - 378	1.5e-08	Pentacotripeptide-repeat region of PRORP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77405
fragaria_vesca	FvH4_2g27550
malus_domestica	MD00G1030400.v1.1
prunus_persica	Prupe.1G492400_v2.0.a1
pyrus_communis	pycom15g30800
rosa_chinensis	RchiOBHm_Chr4g0420811 RchiOBHm_Chr4g0420821
rosa_laevigata	RLG00000007736 RLG00000011697
rosa_multiflora	Rmu_co8321563.1_g000001 Rmu_sc0005104.1_g000002 Rmu_sc0006997.1_g000002 Rmu_sc0006997.1_g000003
rosa_roxburghii	Rroxscaffold_5G00363250
rosa_rugosa	Rorug04G0166200 Rorug04G0166300 Rorug06G0261900
rosa_samantha	Rh4AG227600 Rh4BG230600 Rh4BG230700 Rh4BG230800 Rh4CG242600 Rh4CG242700 Rh6BG381400
rosa_wichuraiana	Rw4G019690 Rw6G032550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	630
Acc36I	ACCTGC	1 cut(s)	1278
AccBSI	CCGCTC	2 cut(s)	201, 498
AccI	GTMKAC	2 cut(s)	1091, 1190
AccII	CGCG	1 cut(s)	234
AccIII	TCCGGA	1 cut(s)	801
AclWI	GGATC	1 cut(s)	1136
AcoI	YGGCCR	2 cut(s)	208, 435
AcsI	RAATTY	5 cut(s)	275, 359, 664, 1057, 1219
AfaI	GTAC	2 cut(s)	964, 1001
AfiI	CCNNNNNNNGG	5 cut(s)	387, 550, 804, 972, 973
AflIII	ACRYGT	1 cut(s)	828
AgsI	TTSAA	9 cut(s)	12, 22, 244, 454, 670, 941, 1037, 1063, 1083
AluBI	AGCT	2 cut(s)	337, 1226
AluI	AGCT	2 cut(s)	337, 1226
Alw21I	GWGCWC	1 cut(s)	294
Alw26I	GTCTC	1 cut(s)	773
AlwI	GGATC	1 cut(s)	1136
Ama87I	CYCGRG	2 cut(s)	338, 1140
Aor13HI	TCCGGA	1 cut(s)	801
AoxI	GGCC	5 cut(s)	208, 301, 435, 675, 795
ApeKI	GCWGC	3 cut(s)	308, 578, 1007
ApoI	RAATTY	5 cut(s)	275, 359, 664, 1057, 1219
ArsI	GACNNNNNNTTYG	2 cut(s)	15, 47
AspA2I	CCTAGG	1 cut(s)	544
AspLEI	GCGC	1 cut(s)	236
AspS9I	GGNCC	2 cut(s)	302, 676
AsuC2I	CCSGG	4 cut(s)	220, 305, 679, 708
AsuHPI	GGTGA	1 cut(s)	1321
AsuII	TTCGAA	1 cut(s)	835
AvaI	CYCGRG	2 cut(s)	338, 1140
AvrII	CCTAGG	1 cut(s)	544
AxyI	CCTNAGG	1 cut(s)	257
BalI	TGGCCA	1 cut(s)	437
Bbv12I	GWGCWC	1 cut(s)	294
BbvI	GCAGC	3 cut(s)	320, 565, 994
BccI	CCATC	4 cut(s)	415, 691, 806, 1160
BceAI	ACGGC	1 cut(s)	118
BclI	TGATCA	1 cut(s)	888
BcnI	CCSGG	4 cut(s)	220, 305, 679, 708
BcoDI	GTCTC	1 cut(s)	773
BfaI	CTAG	2 cut(s)	545, 1182
BfmI	CTRYAG	1 cut(s)	1008
BfuAI	ACCTGC	1 cut(s)	1278
BglII	AGATCT	1 cut(s)	319
BlnI	CCTAGG	1 cut(s)	544
Bme1390I	CCNGG	4 cut(s)	220, 305, 679, 708
BmeT110I	CYCGRG	2 cut(s)	338, 1140
BmgT120I	GGNCC	2 cut(s)	302, 676
BmiI	GGNNCC	1 cut(s)	224
BmrFI	CCNGG	4 cut(s)	220, 305, 679, 708
BmrI	ACTGGG	1 cut(s)	379
BmsI	GCATC	4 cut(s)	559, 707, 1041, 1126
BmuI	ACTGGG	1 cut(s)	379
BpmI	CTGGAG	1 cut(s)	1248
Bpu14I	TTCGAA	1 cut(s)	835
BpuMI	CCSGG	4 cut(s)	220, 305, 679, 708
BsaAI	YACGTR	1 cut(s)	1194
BsaBI	GATNNNNATC	1 cut(s)	78
BsaJI	CCNNGG	3 cut(s)	134, 544, 707
BsaWI	WCCGGW	2 cut(s)	801, 912
Bsc4I	CCNNNNNNNGG	5 cut(s)	387, 550, 804, 972, 973
Bse1I	ACTGG	2 cut(s)	190, 374
Bse21I	CCTNAGG	1 cut(s)	257
Bse3DI	GCAATG	1 cut(s)	604
Bse8I	GATNNNNATC	1 cut(s)	78
BseAI	TCCGGA	1 cut(s)	801
BseDI	CCNNGG	3 cut(s)	134, 544, 707
BseGI	GGATG	3 cut(s)	622, 733, 798
BseJI	GATNNNNATC	1 cut(s)	78
BseLI	CCNNNNNNNGG	5 cut(s)	387, 550, 804, 972, 973
BseMI	GCAATG	1 cut(s)	604
BseMII	CTCAG	2 cut(s)	143, 271
BseNI	ACTGG	2 cut(s)	190, 374
BseX3I	CGGCCG	1 cut(s)	208
BseXI	GCAGC	3 cut(s)	320, 565, 994
Bsh1236I	CGCG	1 cut(s)	234
Bsh1285I	CGRYCG	1 cut(s)	211
BshFI	GGCC	5 cut(s)	210, 303, 437, 677, 797
BsiEI	CGRYCG	1 cut(s)	211
BsiHKAI	GWGCWC	1 cut(s)	294
BsiHKCI	CYCGRG	2 cut(s)	338, 1140
BsiSI	CCGG	8 cut(s)	219, 305, 614, 679, 707, 725, 802, 913
BslFI	GGGAC	1 cut(s)	178
BslI	CCNNNNNNNGG	5 cut(s)	387, 550, 804, 972, 973
BsmAI	GTCTC	1 cut(s)	773
BsmBI	CGTCTC	1 cut(s)	773
BsmFI	GGGAC	1 cut(s)	178
BsmI	GAATGC	1 cut(s)	1077
BsnI	GGCC	5 cut(s)	210, 303, 437, 677, 797
BsoBI	CYCGRG	2 cut(s)	338, 1140
Bsp119I	TTCGAA	1 cut(s)	835
Bsp1286I	GDGCHC	1 cut(s)	294
Bsp13I	TCCGGA	1 cut(s)	801
Bsp143I	GATC	3 cut(s)	319, 888, 1128
BspANI	GGCC	5 cut(s)	210, 303, 437, 677, 797
BspCNI	CTCAG	2 cut(s)	142, 270
BspEI	TCCGGA	1 cut(s)	801
BspFNI	CGCG	1 cut(s)	234
BspLI	GGNNCC	1 cut(s)	224
BspMAI	CTGCAG	1 cut(s)	1012
BspMI	ACCTGC	1 cut(s)	1278
BspPI	GGATC	1 cut(s)	1136
BspT104I	TTCGAA	1 cut(s)	835
BsrBI	CCGCTC	2 cut(s)	201, 498
BsrDI	GCAATG	1 cut(s)	604
BsrI	ACTGG	2 cut(s)	190, 374
BssECI	CCNNGG	3 cut(s)	134, 544, 707
BssMI	GATC	3 cut(s)	319, 888, 1128
BssNAI	GTATAC	2 cut(s)	1092, 1191
BssT1I	CCWWGG	1 cut(s)	544
Bst1107I	GTATAC	2 cut(s)	1092, 1191
Bst4CI	ACNGT	2 cut(s)	8, 1277
Bst6I	CTCTTC	1 cut(s)	482
BstAPI	GCANNNNNTGC	1 cut(s)	578
BstBAI	YACGTR	1 cut(s)	1194
BstBI	TTCGAA	1 cut(s)	835
BstC8I	GCNNGC	3 cut(s)	503, 611, 722
BstDEI	CTNAG	2 cut(s)	129, 257
BstDSI	CCRYGG	1 cut(s)	134
BstENI	CCTNNNNNAGG	1 cut(s)	548
BstF5I	GGATG	3 cut(s)	622, 733, 798
BstFNI	CGCG	1 cut(s)	234
BstHHI	GCGC	1 cut(s)	236
BstKTI	GATC	3 cut(s)	322, 891, 1131
BstMAI	GTCTC	1 cut(s)	773
BstMBI	GATC	3 cut(s)	319, 888, 1128
BstMCI	CGRYCG	1 cut(s)	211
BstMWI	GCNNNNNNNGC	8 cut(s)	92, 95, 104, 107, 116, 119, 207, 578
BstNSI	RCATGY	2 cut(s)	832, 844
BstSCI	CCNGG	4 cut(s)	218, 303, 677, 706
BstSFI	CTRYAG	1 cut(s)	1008
BstSNI	TACGTA	1 cut(s)	1194
BstUI	CGCG	1 cut(s)	234
BstV1I	GCAGC	3 cut(s)	320, 565, 994
BstX2I	RGATCY	1 cut(s)	319
BstYI	RGATCY	1 cut(s)	319
BstZ17I	GTATAC	2 cut(s)	1092, 1191
BstZI	CGGCCG	1 cut(s)	208
Bsu36I	CCTNAGG	1 cut(s)	257
BsuRI	GGCC	5 cut(s)	210, 303, 437, 677, 797
BtgI	CCRYGG	1 cut(s)	134
BtsCI	GGATG	3 cut(s)	622, 733, 798
BtsIMutI	CAGTG	1 cut(s)	856
BveI	ACCTGC	1 cut(s)	1278
Cac8I	GCNNGC	3 cut(s)	503, 611, 722
CfoI	GCGC	1 cut(s)	236
Cfr13I	GGNCC	2 cut(s)	302, 676
Csp6I	GTAC	2 cut(s)	963, 1000
CviAII	CATG	5 cut(s)	52, 829, 841, 1106, 1345
CviQI	GTAC	2 cut(s)	963, 1000
DdeI	CTNAG	2 cut(s)	129, 257
DpnI	GATC	3 cut(s)	321, 890, 1130
DpnII	GATC	3 cut(s)	319, 888, 1128
EaeI	YGGCCR	2 cut(s)	208, 435
EagI	CGGCCG	1 cut(s)	208
Eam1104I	CTCTTC	1 cut(s)	482
EarI	CTCTTC	1 cut(s)	482
EciI	GGCGGA	4 cut(s)	82, 94, 106, 215
EclXI	CGGCCG	1 cut(s)	208
Eco105I	TACGTA	1 cut(s)	1194
Eco130I	CCWWGG	1 cut(s)	544
Eco52I	CGGCCG	1 cut(s)	208
Eco81I	CCTNAGG	1 cut(s)	257
Eco88I	CYCGRG	2 cut(s)	338, 1140
EcoNI	CCTNNNNNAGG	1 cut(s)	548
EcoRI	GAATTC	1 cut(s)	359
EcoT14I	CCWWGG	1 cut(s)	544
EcoT22I	ATGCAT	1 cut(s)	574
ErhI	CCWWGG	1 cut(s)	544
Esp3I	CGTCTC	1 cut(s)	773
FaeI	CATG	5 cut(s)	55, 832, 844, 1109, 1348
FaqI	GGGAC	1 cut(s)	178
FatI	CATG	5 cut(s)	51, 828, 840, 1105, 1344
FauI	CCCGC	3 cut(s)	206, 494, 729
FbaI	TGATCA	1 cut(s)	888
FblI	GTMKAC	2 cut(s)	1091, 1190
FokI	GGATG	3 cut(s)	629, 740, 785
FspBI	CTAG	2 cut(s)	545, 1182
GlaI	GCGC	1 cut(s)	235
GsuI	CTGGAG	1 cut(s)	1248
HaeIII	GGCC	5 cut(s)	210, 303, 437, 677, 797
HapII	CCGG	8 cut(s)	219, 305, 614, 679, 707, 725, 802, 913
HhaI	GCGC	1 cut(s)	236
Hin1II	CATG	5 cut(s)	55, 832, 844, 1109, 1348
Hin6I	GCGC	1 cut(s)	234
HinP1I	GCGC	1 cut(s)	234
HincII	GTYRAC	1 cut(s)	34
HindII	GTYRAC	1 cut(s)	34
HinfI	GANTC	6 cut(s)	74, 146, 180, 266, 986, 1324
HpaII	CCGG	8 cut(s)	219, 305, 614, 679, 707, 725, 802, 913
HphI	GGTGA	1 cut(s)	1321
Hpy166II	GTNNAC	5 cut(s)	34, 138, 735, 1092, 1191
Hpy188I	TCNGA	1 cut(s)	346
Hpy188III	TCNNGA	3 cut(s)	323, 802, 863
Hpy8I	GTNNAC	5 cut(s)	34, 138, 735, 1092, 1191
Hpy99I	CGWCG	1 cut(s)	209
HpyAV	CCTTC	5 cut(s)	79, 548, 787, 1119, 1300
HpyCH4III	ACNGT	2 cut(s)	8, 1277
HpyCH4IV	ACGT	2 cut(s)	288, 1193
HpyCH4V	TGCA	8 cut(s)	414, 572, 609, 654, 903, 1010, 1054, 1214
HpyF10VI	GCNNNNNNNGC	8 cut(s)	92, 95, 104, 107, 116, 119, 207, 578
HpyF3I	CTNAG	2 cut(s)	129, 257
HpySE526I	ACGT	2 cut(s)	288, 1193
Hsp92II	CATG	5 cut(s)	55, 832, 844, 1109, 1348
HspAI	GCGC	1 cut(s)	234
Kpn2I	TCCGGA	1 cut(s)	801
Ksp22I	TGATCA	1 cut(s)	888
Kzo9I	GATC	3 cut(s)	319, 888, 1128
LmnI	GCTCC	4 cut(s)	206, 241, 495, 691
Lsp1109I	GCAGC	3 cut(s)	320, 565, 994
LweI	GCATC	4 cut(s)	559, 707, 1041, 1126
MaeI	CTAG	2 cut(s)	545, 1182
MaeII	ACGT	2 cut(s)	288, 1193
MaeIII	GTNAC	4 cut(s)	508, 523, 871, 976
MalI	GATC	3 cut(s)	321, 890, 1130
MbiI	CCGCTC	2 cut(s)	201, 498
MboI	GATC	3 cut(s)	319, 888, 1128
MboII	GAAGA	5 cut(s)	175, 178, 499, 953, 1060
MflI	RGATCY	1 cut(s)	319
MhlI	GDGCHC	1 cut(s)	294
MlsI	TGGCCA	1 cut(s)	437
MluCI	AATT	9 cut(s)	165, 275, 359, 664, 880, 1057, 1199, 1219, 1237
MluNI	TGGCCA	1 cut(s)	437
MlyI	GAGTC	2 cut(s)	140, 1333
MmeI	TCCRAC	3 cut(s)	324, 669, 909
Mox20I	TGGCCA	1 cut(s)	437
Mph1103I	ATGCAT	1 cut(s)	574
MroI	TCCGGA	1 cut(s)	801
MscI	TGGCCA	1 cut(s)	437
MseI	TTAA	2 cut(s)	564, 1014
Msp20I	TGGCCA	1 cut(s)	437
MspA1I	CMGCKG	1 cut(s)	1226
MspI	CCGG	8 cut(s)	219, 305, 614, 679, 707, 725, 802, 913
MspR9I	CCNGG	4 cut(s)	220, 305, 679, 708
Mva1269I	GAATGC	1 cut(s)	1077
MvnI	CGCG	1 cut(s)	234
MwoI	GCNNNNNNNGC	8 cut(s)	92, 95, 104, 107, 116, 119, 207, 578
NciI	CCSGG	4 cut(s)	220, 305, 679, 708
NdeII	GATC	3 cut(s)	319, 888, 1128
NlaIII	CATG	5 cut(s)	55, 832, 844, 1109, 1348
NlaIV	GGNNCC	1 cut(s)	224
NmeAIII	GCCGAG	1 cut(s)	279
NmuCI	GTSAC	1 cut(s)	871
NsiI	ATGCAT	1 cut(s)	574
NspI	RCATGY	2 cut(s)	832, 844
NspV	TTCGAA	1 cut(s)	835
PciI	ACATGT	1 cut(s)	828
PctI	GAATGC	1 cut(s)	1077
PfeI	GAWTC	4 cut(s)	74, 180, 266, 986
PleI	GAGTC	2 cut(s)	140, 1332
PpsI	GAGTC	2 cut(s)	140, 1332
Ppu21I	YACGTR	1 cut(s)	1194
PscI	ACATGT	1 cut(s)	828
PsiI	TTATAA	1 cut(s)	630
PspN4I	GGNNCC	1 cut(s)	224
PspPI	GGNCC	2 cut(s)	302, 676
PstI	CTGCAG	1 cut(s)	1012
PsuI	RGATCY	1 cut(s)	319
PvuII	CAGCTG	1 cut(s)	1226
RsaI	GTAC	2 cut(s)	964, 1001
RsaNI	GTAC	2 cut(s)	963, 1000
SaqAI	TTAA	2 cut(s)	564, 1014
Sau3AI	GATC	3 cut(s)	319, 888, 1128
Sau96I	GGNCC	2 cut(s)	302, 676
SchI	GAGTC	2 cut(s)	140, 1333
ScrFI	CCNGG	4 cut(s)	220, 305, 679, 708
SduI	GDGCHC	1 cut(s)	294
SfaNI	GCATC	4 cut(s)	559, 707, 1041, 1126
SfcI	CTRYAG	1 cut(s)	1008
SfuI	TTCGAA	1 cut(s)	835
SnaBI	TACGTA	1 cut(s)	1194
Sse9I	AATT	9 cut(s)	165, 275, 359, 664, 880, 1057, 1199, 1219, 1237
SspMI	CTAG	2 cut(s)	545, 1182
StyD4I	CCNGG	4 cut(s)	218, 303, 677, 706
StyI	CCWWGG	1 cut(s)	544
TaaI	ACNGT	2 cut(s)	8, 1277
TaiI	ACGT	2 cut(s)	291, 1196
TaqI	TCGA	7 cut(s)	72, 324, 357, 375, 485, 835, 1175
TasI	AATT	9 cut(s)	165, 275, 359, 664, 880, 1057, 1199, 1219, 1237
TfiI	GAWTC	4 cut(s)	74, 180, 266, 986
Tru1I	TTAA	2 cut(s)	564, 1014
Tru9I	TTAA	2 cut(s)	564, 1014
TscAI	CASTG	1 cut(s)	856
TseFI	GTSAC	1 cut(s)	871
TseI	GCWGC	3 cut(s)	308, 578, 1007
Tsp45I	GTSAC	1 cut(s)	871
TspDTI	ATGAA	6 cut(s)	17, 593, 608, 962, 1094, 1319
TspGWI	ACGGA	3 cut(s)	193, 421, 1083
TspRI	CASTG	1 cut(s)	856
XagI	CCTNNNNNAGG	1 cut(s)	548
XapI	RAATTY	5 cut(s)	275, 359, 664, 1057, 1219
XceI	RCATGY	2 cut(s)	832, 844
XmaJI	CCTAGG	1 cut(s)	544
XmiI	GTMKAC	2 cut(s)	1091, 1190
XspI	CTAG	2 cut(s)	545, 1182
Zsp2I	ATGCAT	1 cut(s)	574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.