Rh4AG227600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006654
Physical Location & Seq
Forward (+)
52871955 .. 52873263
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG227600.1

Sequence Viewer

Length: 348 bp
ATGGCTACCTCAACAGTCAAGTGCGCGGCTGCACTCCTTTGTTTACAAATGGAGGCAGCTCCAAGGGAAGGCACAGCTGGTCTACTAACTAATGTTCCAAAAGTTACTGCAGTGTTTGAGTCTTGGCATAGATGCATATCTCATTTGTTGGTGCAGCGAGTGCTACATGCGCTTGGTTATGCTTGTAGGCAGTATTTGTTACATGCAATGATATTGACATTGTCGATTCCTGAGATTCCAAGAATTGAGGGCATAGTTTCTGAGCTTAAAGGTTCTGGTGTACCTACTTTAGCCACTGCTGCTTTTCATGTGGCTGTGGAGTTTCAGCGGCTTCCTCGCTGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

115

Amino Acids

12.49

Weight (kDa)

8.74

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_RUK PF24970 7 - 114 1e-36 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 82
AccII CGCG 1 cut(s) 26
AciI CCGC 2 cut(s) 26, 328
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 1 cut(s) 68
AluBI AGCT 3 cut(s) 59, 77, 265
AluI AGCT 3 cut(s) 59, 77, 265
ApeKI GCWGC 5 cut(s) 29, 56, 154, 299, 339
AspLEI GCGC 2 cut(s) 26, 172
BbvI GCAGC 5 cut(s) 16, 68, 166, 286, 326
BfmI CTRYAG 1 cut(s) 108
BisI GCNGC 7 cut(s) 27, 30, 57, 155, 300, 329, 340
BlsI GCNGC 7 cut(s) 28, 31, 58, 156, 301, 330, 341
BmsI GCATC 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 62
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 62
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 222, 252
BseXI GCAGC 5 cut(s) 16, 68, 166, 286, 326
BsgI GTGCAG 2 cut(s) 15, 173
Bsh1236I CGCG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 68
BspACI CCGC 2 cut(s) 26, 328
BspCNI CTCAG 2 cut(s) 223, 253
BspFNI CGCG 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 213
BssECI CCNNGG 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 231, 261
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 2 cut(s) 26, 172
BstMWI GCNNNNNNNGC 3 cut(s) 160, 169, 299
BstNSI RCATGY 2 cut(s) 170, 206
BstSFI CTRYAG 1 cut(s) 108
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 5 cut(s) 16, 68, 166, 286, 326
BtsI GCAGTG 2 cut(s) 117, 294
BtsIMutI CAGTG 2 cut(s) 117, 294
CfoI GCGC 2 cut(s) 26, 172
Csp6I GTAC 1 cut(s) 281
CviAII CATG 3 cut(s) 167, 203, 308
CviJI RGCY 8 cut(s) 5, 29, 59, 77, 265, 293, 314, 331
CviKI_1 RGCY 8 cut(s) 5, 29, 59, 77, 265, 293, 314, 331
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 2 cut(s) 231, 261
Eco130I CCWWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 62
EcoT22I ATGCAT 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 3 cut(s) 170, 206, 311
FaiI YATR 7 cut(s) 129, 137, 168, 180, 204, 254, 309
FatI CATG 3 cut(s) 166, 202, 307
FblI GTMKAC 1 cut(s) 82
Fnu4HI GCNGC 7 cut(s) 27, 30, 57, 155, 300, 329, 340
Fsp4HI GCNGC 7 cut(s) 27, 30, 57, 155, 300, 329, 340
GlaI GCGC 2 cut(s) 25, 171
GluI GCNGC 7 cut(s) 27, 30, 57, 155, 300, 329, 340
HhaI GCGC 2 cut(s) 26, 172
Hin1II CATG 3 cut(s) 170, 206, 311
Hin6I GCGC 2 cut(s) 24, 170
HinP1I GCGC 2 cut(s) 24, 170
HinfI GANTC 3 cut(s) 119, 226, 235
Hpy166II GTNNAC 3 cut(s) 44, 83, 281
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 3 cut(s) 44, 83, 281
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 5 cut(s) 32, 110, 135, 154, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 169, 299
HpyF3I CTNAG 2 cut(s) 231, 261
Hsp92II CATG 3 cut(s) 170, 206, 311
HspAI GCGC 2 cut(s) 24, 170
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 3 cut(s) 63, 243, 261
Lsp1109I GCAGC 5 cut(s) 16, 68, 166, 286, 326
LweI GCATC 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 103, 198
MluCI AATT 1 cut(s) 243
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 4 cut(s) 19, 46, 241, 345
Mph1103I ATGCAT 1 cut(s) 137
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 77, 328
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 160, 169, 299
NlaIII CATG 3 cut(s) 170, 206, 311
NsiI ATGCAT 1 cut(s) 137
NspI RCATGY 2 cut(s) 170, 206
PfeI GAWTC 2 cut(s) 226, 235
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 7 cut(s) 28, 31, 58, 156, 301, 330, 341
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PstI CTGCAG 1 cut(s) 112
PsyI GACNNNGTC 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 7 cut(s) 27, 30, 57, 155, 300, 329, 340
SchI GAGTC 1 cut(s) 128
SetI ASST 6 cut(s) 11, 61, 79, 267, 274, 286
SfaNI GCATC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 108
Sse9I AATT 1 cut(s) 243
SsiI CCGC 2 cut(s) 26, 328
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 224
TasI AATT 1 cut(s) 243
TauI GCSGC 2 cut(s) 29, 331
TfiI GAWTC 2 cut(s) 226, 235
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 117, 301
TseI GCWGC 5 cut(s) 29, 56, 154, 299, 339
TspDTI ATGAA 1 cut(s) 296
TspRI CASTG 2 cut(s) 117, 301
Tth111I GACNNNGTC 1 cut(s) 220
XceI RCATGY 2 cut(s) 170, 206
XmiI GTMKAC 1 cut(s) 82
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.