Rw4G019690

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006654
Physical Location & Seq
Forward (+)
43155000 .. 43156663
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019690.1

Sequence Viewer

Length: 1320 bp
ATGAAGCACTCAAAACCTCTAGTGAACCAAGTCCTGACAGCCATGCTCAAAAACCAACCTTTTGACCCAAACCCCTCAGTTTCAGCCACCACAACTCAACCATGGACCTCCGACTCAGTCTCCCAAGTCCTGACCTCCATCCCCAGATTCTTCTTCCAGTCCCCTCGCTCCATAGGTCGCCAAACCGGCTTCCGGCACCGCGCTCCGTTAAAGCAACGCCATCTCAGGCAAGAAACATATGAATTCCACAACGACGGAGCTGCCCACAGAGACCTCAGCAAGGTCAATCTGGGAGTGGACAAAGCCCTCGATTTTTACTACTGGGTCGAAACCCATTTCGGGTTCCACCACAATGAGCGAACTTGCAGAGACATGGCTGTCGTTCTGGCTAAAGGGAATCGGTTGAAGGCCCTCTGGGATTTTCTGAAAGACATGTCGAAGAGAGGGAGTGGTGAAAGCCCTCCTCTTGTAACGACACAGTCCATCACCTGTTTGATGAAATGCCTAGGAGAAGAGGGATTGGTCAATGAAGCATTGGCGGCTTTCTATAGGATGAAGCAGTTTCATTGCAAACCGGATGTGTATGCTTACAACACTATTATTTACGCGCTTTGCAGGGTTGGGAATTTCAAGAAGGCTCGGTTTTTGCTGGAGCAGATGGAGCTGCCGGGTTTTAGATGCCCGCCGGATACATTCACTTACACGATTTTGATAAGCTCTTATTGTAGATATGGGTTGGAGACTGGGTGCAGGAAGGCCACTAGGAGGAGGTTGTGGGAAGCAAACCACATGTTTCGGAATATGCTCTTTAGAGGGTTTGTTCCTGATGTTGTTACTTACAATGCTTTGATCAATGGTTGTTGCAAGACTTACAGGATAGAGAGGGCGTTGGAGTTGTTTGGGGATATGAAGAAGAGGGATTGTACCCCCAACCGCGTTACTTATGATTCGTTTATTAGATACTTTGCTGCTGTTAATGAGATTGACAAGGCTGTGGATATGTTAAGAAGGATGCAGAATATGAATCATGGATTGGCCACTTCAAGTTCGTATACTCCAATCATTCATGCCATGTGTGAAGCAGGAAGGGTTGCAGAGGCTTGGGGTTTTCTTGTTGAGTTGGTTGATGGAGGGTCAATACCTAGAGAATATACTTACAAATTAGTTTGCAATGCAATGAGTTCAGCTGGAGAGGTCAGTTTACTTGATGATGGCCTGCGAAAAAGAATAGAAGATGGTATGCAGAGGAGATATAAGCACATAATGAAGGTGAAGCCAATAATGACTCGCAAAGGGTATGATAGCATGGTGGAAACATGA

Protein Analysis

439

Amino Acids

50.55

Weight (kDa)

9.38

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 206 2.8e-09 PPR repeat family
PPR_1 PF12854 189 - 221 1.6e-09 PPR repeat
PPR_2 PF13041 192 - 243 4.7e-13 PPR repeat family
PPR_1 PF12854 227 - 245 7.2e-06 PPR repeat
PPR_2 PF13041 256 - 289 1.9e-08 PPR repeat family
PPR_3 PF13812 264 - 322 7.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 271 - 304 1.9e-11 PPR repeat
PPR_2 PF13041 275 - 322 2.3e-17 PPR repeat family
PPR_long PF17177 277 - 372 4.9e-08 Pentacotripeptide-repeat region of PRORP
PPR PF01535 278 - 308 3.3e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 1052
AccII CGCG 3 cut(s) 201, 608, 936
AciI CCGC 4 cut(s) 199, 539, 683, 934
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 2 cut(s) 242, 625
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 4 cut(s) 192, 280, 339, 765
AflIII ACRYGT 2 cut(s) 432, 789
AgsI TTSAA 3 cut(s) 406, 631, 1044
AjuI GAANNNNNNNTTGG 2 cut(s) 1016, 1048
AluBI AGCT 4 cut(s) 260, 664, 717, 1187
AluI AGCT 4 cut(s) 260, 664, 717, 1187
Alw26I GTCTC 4 cut(s) 124, 264, 363, 734
AoxI GGCC 4 cut(s) 408, 756, 1035, 1213
ApeKI GCWGC 3 cut(s) 260, 664, 968
ApoI RAATTY 2 cut(s) 242, 625
AspA2I CCTAGG 1 cut(s) 505
AspLEI GCGC 2 cut(s) 203, 610
AspS9I GGNCC 2 cut(s) 105, 409
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 3 cut(s) 464, 478, 1282
AvaII GGWCC 1 cut(s) 105
AvrII CCTAGG 1 cut(s) 505
BalI TGGCCA 1 cut(s) 1037
BanI GGYRCC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 3 cut(s) 247, 651, 955
BccI CCATC 7 cut(s) 146, 228, 491, 652, 1121, 1205, 1229
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 4 cut(s) 124, 264, 363, 734
BfaI CTAG 4 cut(s) 20, 506, 762, 1143
BfmI CTRYAG 1 cut(s) 547
BfuI GTATCC 1 cut(s) 682
BglI GCCNNNNNGGC 1 cut(s) 186
BisI GCNGC 4 cut(s) 261, 540, 665, 969
BlnI CCTAGG 1 cut(s) 505
BlsI GCNGC 4 cut(s) 262, 541, 666, 970
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 105, 409
BmiI GGNNCC 2 cut(s) 197, 344
BmrFI CCNGG 1 cut(s) 669
BmrI ACTGGG 2 cut(s) 331, 753
BmsI GCATC 2 cut(s) 668, 1002
BmuI ACTGGG 2 cut(s) 331, 753
BpmI CTGGAG 2 cut(s) 671, 1209
Bpu10I CCTNAGC 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 669
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 101, 505
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 731, 761
Bsc4I CCNNNNNNNGG 4 cut(s) 192, 280, 339, 765
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 157, 326, 748
Bse3DI GCAATG 3 cut(s) 565, 1177, 1182
BseDI CCNNGG 2 cut(s) 101, 505
BseGI GGATG 4 cut(s) 138, 558, 583, 1017
BseLI CCNNNNNNNGG 4 cut(s) 192, 280, 339, 765
BseMI GCAATG 3 cut(s) 565, 1177, 1182
BseMII CTCAG 4 cut(s) 90, 129, 238, 289
BseNI ACTGG 3 cut(s) 157, 326, 748
BseRI GAGGAG 3 cut(s) 453, 781, 1261
BseXI GCAGC 3 cut(s) 247, 651, 955
BsgI GTGCAG 1 cut(s) 769
Bsh1236I CGCG 3 cut(s) 201, 608, 936
BshFI GGCC 4 cut(s) 410, 758, 1037, 1215
BshNI GGYRCC 1 cut(s) 195
BsiSI CCGG 5 cut(s) 186, 193, 575, 668, 686
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 4 cut(s) 192, 280, 339, 765
BsmAI GTCTC 4 cut(s) 124, 264, 363, 734
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 4 cut(s) 410, 758, 1037, 1215
Bso31I GGTCTC 1 cut(s) 264
Bsp143I GATC 1 cut(s) 849
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 4 cut(s) 199, 539, 683, 934
BspANI GGCC 4 cut(s) 410, 758, 1037, 1215
BspCNI CTCAG 4 cut(s) 89, 128, 237, 288
BspFNI CGCG 3 cut(s) 201, 608, 936
BspLI GGNNCC 2 cut(s) 197, 344
BspT107I GGYRCC 1 cut(s) 195
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 3 cut(s) 565, 1177, 1182
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 3 cut(s) 157, 326, 748
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 101, 505
BssMI GATC 1 cut(s) 849
BssNAI GTATAC 1 cut(s) 1053
BssT1I CCWWGG 2 cut(s) 101, 505
Bst1107I GTATAC 1 cut(s) 1053
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 3 cut(s) 434, 507, 908
BstC8I GCNNGC 2 cut(s) 683, 1217
BstDEI CTNAG 4 cut(s) 76, 115, 224, 275
BstDSI CCRYGG 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 278
BstF5I GGATG 4 cut(s) 138, 558, 583, 1017
BstFNI CGCG 3 cut(s) 201, 608, 936
BstHHI GCGC 2 cut(s) 203, 610
BstKTI GATC 1 cut(s) 852
BstMAI GTCTC 4 cut(s) 124, 264, 363, 734
BstMBI GATC 1 cut(s) 849
BstMWI GCNNNNNNNGC 3 cut(s) 186, 539, 661
BstNSI RCATGY 2 cut(s) 436, 793
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 547
BstUI CGCG 3 cut(s) 201, 608, 936
BstV1I GCAGC 3 cut(s) 247, 651, 955
BstZ17I GTATAC 1 cut(s) 1053
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 4 cut(s) 410, 758, 1037, 1215
BtgI CCRYGG 1 cut(s) 101
BtsCI GGATG 4 cut(s) 138, 558, 583, 1017
Cac8I GCNNGC 2 cut(s) 683, 1217
CfoI GCGC 2 cut(s) 203, 610
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 105, 409
Csp6I GTAC 1 cut(s) 924
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 4 cut(s) 76, 115, 224, 275
DpnI GATC 1 cut(s) 851
DpnII GATC 1 cut(s) 849
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 3 cut(s) 434, 507, 908
EarI CTCTTC 3 cut(s) 434, 507, 908
Eco130I CCWWGG 2 cut(s) 101, 505
Eco31I GGTCTC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 105
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 409
EcoRI GAATTC 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 101, 505
ErhI CCWWGG 2 cut(s) 101, 505
FaqI GGGAC 1 cut(s) 145
FauI CCCGC 1 cut(s) 690
FauNDI CATATG 1 cut(s) 238
FbaI TGATCA 1 cut(s) 849
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 4 cut(s) 261, 540, 665, 969
FokI GGATG 4 cut(s) 125, 565, 590, 1024
Fsp4HI GCNGC 4 cut(s) 261, 540, 665, 969
FspBI CTAG 4 cut(s) 20, 506, 762, 1143
GlaI GCGC 2 cut(s) 202, 609
GluI GCNGC 4 cut(s) 261, 540, 665, 969
GsuI CTGGAG 2 cut(s) 671, 1209
HaeIII GGCC 4 cut(s) 410, 758, 1037, 1215
HapII CCGG 5 cut(s) 186, 193, 575, 668, 686
HhaI GCGC 2 cut(s) 203, 610
Hin6I GCGC 2 cut(s) 201, 608
HinP1I GCGC 2 cut(s) 201, 608
HinfI GANTC 6 cut(s) 113, 147, 397, 947, 1024, 1285
HpaII CCGG 5 cut(s) 186, 193, 575, 668, 686
HphI GGTGA 3 cut(s) 464, 478, 1282
Hpy166II GTNNAC 4 cut(s) 25, 298, 1053, 1202
Hpy188I TCNGA 3 cut(s) 112, 426, 798
Hpy188III TCNNGA 4 cut(s) 34, 130, 631, 824
Hpy8I GTNNAC 4 cut(s) 25, 298, 1053, 1202
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 6 cut(s) 400, 628, 748, 1002, 1080, 1261
HpyCH4III ACNGT 1 cut(s) 480
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 539, 661
HpyF3I CTNAG 4 cut(s) 76, 115, 224, 275
HspAI GCGC 2 cut(s) 201, 608
Ksp22I TGATCA 1 cut(s) 849
Kzo9I GATC 1 cut(s) 849
LmnI GCTCC 5 cut(s) 173, 208, 257, 652, 661
Lsp1109I GCAGC 3 cut(s) 247, 651, 955
LweI GCATC 2 cut(s) 668, 1002
MaeI CTAG 4 cut(s) 20, 506, 762, 1143
MaeIII GTNAC 3 cut(s) 469, 832, 937
MalI GATC 1 cut(s) 851
MboI GATC 1 cut(s) 849
MboII GAAGA 7 cut(s) 142, 145, 451, 524, 922, 925, 1244
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 3 cut(s) 242, 625, 1160
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 2 cut(s) 107, 1279
MmeI TCCRAC 3 cut(s) 135, 717, 870
Mox20I TGGCCA 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 3 cut(s) 209, 975, 1004
MslI CAYNNNNRTG 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 1 cut(s) 1187
MspI CCGG 5 cut(s) 186, 193, 575, 668, 686
MspR9I CCNGG 1 cut(s) 669
MvnI CGCG 3 cut(s) 201, 608, 936
MwoI GCNNNNNNNGC 3 cut(s) 186, 539, 661
NciI CCSGG 1 cut(s) 669
NcoI CCATGG 1 cut(s) 101
NdeI CATATG 1 cut(s) 238
NdeII GATC 1 cut(s) 849
NlaIV GGNNCC 2 cut(s) 197, 344
NspI RCATGY 2 cut(s) 436, 793
PciI ACATGT 2 cut(s) 432, 789
PfeI GAWTC 4 cut(s) 147, 397, 947, 1024
PflFI GACNNNGTC 2 cut(s) 116, 478
PkrI GCNGC 4 cut(s) 262, 541, 666, 970
PleI GAGTC 2 cut(s) 107, 1279
PpsI GAGTC 2 cut(s) 107, 1279
PscI ACATGT 2 cut(s) 432, 789
PspN4I GGNNCC 2 cut(s) 197, 344
PspPI GGNCC 2 cut(s) 105, 409
PsyI GACNNNGTC 2 cut(s) 116, 478
PvuII CAGCTG 1 cut(s) 1187
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 3 cut(s) 209, 975, 1004
SatI GCNGC 4 cut(s) 261, 540, 665, 969
Sau3AI GATC 1 cut(s) 849
Sau96I GGNCC 2 cut(s) 105, 409
SchI GAGTC 2 cut(s) 107, 1279
ScrFI CCNGG 1 cut(s) 669
SfaNI GCATC 2 cut(s) 668, 1002
SfcI CTRYAG 1 cut(s) 547
SinI GGWCC 1 cut(s) 105
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 3 cut(s) 242, 625, 1160
SsiI CCGC 4 cut(s) 199, 539, 683, 934
SspMI CTAG 4 cut(s) 20, 506, 762, 1143
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 2 cut(s) 101, 505
TaaI ACNGT 1 cut(s) 480
TaqI TCGA 3 cut(s) 309, 327, 437
TasI AATT 3 cut(s) 242, 625, 1160
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 4 cut(s) 147, 397, 947, 1024
Tru1I TTAA 3 cut(s) 209, 975, 1004
Tru9I TTAA 3 cut(s) 209, 975, 1004
TseI GCWGC 3 cut(s) 260, 664, 968
TspGWI ACGGA 2 cut(s) 195, 270
Tth111I GACNNNGTC 2 cut(s) 116, 478
VpaK11BI GGWCC 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 278
XapI RAATTY 2 cut(s) 242, 625
XceI RCATGY 2 cut(s) 436, 793
XmaJI CCTAGG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 1052
XspI CTAG 4 cut(s) 20, 506, 762, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.