RchiOBHm_Chr4g0420811

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006654
Physical Location & Seq
Forward (+)
46518467 .. 46519888
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39052

Sequence Viewer

Length: 1356 bp
ATGAAGCACTCAAAACCTCTGAACCGCAATTACACACCACTAGTGAACCAAGTCCTGACGGCCATGCTCAAAAACCAACCTTTTGACCCAAACCCCTCAGTTTCAGCCACCACAACTCAACCATGGACCTCCGACTCAGTCTCCCAAGTCCTAACCTCCATCCCCAGATTCTTCTTCCAGTCCCCTCGCTCCATAGGTCGCCAAGCCGGCTTCCGGCACCGCGCTCCGTTAAAGCAACGCCATCTCAGGCGAGAAACATATGAATTCCACAACGACGTGCTCATTCTTGGCCCAGCAGCTGCCCACAGAGACCTCAGCAAGGTCAATCTGGGAGTGGACAGAGCCCTCGATTTTTACTACTGGGTCGAAACCCATTTCGGGTTCAACCACAATGAGCGAACTTGCAGAGACATGGCTGTCGTTTTGGCTAAAGGGAATCGGTTGAAGGCCCTCTGGGATTTTCTGAAAGACATGTCGAAGAGAGGGGGTAGTGAAAGCCCTCTTGTAACGACACAGTCGATCACCTGTTTGATGAAATGCCTAGGAGAAGAGGGATTGGTCAACGAAGCATTGGCGGCTTTCTATAGGATGAAGCAGTTTCATTGCAAACCGGATGCGTATGCTTACAACACTATTATTTACGCGCTTTGCAGAGTTGGGGATTTCAAGAAGGCTCGGTTTTTGCTGGAGCAGATGGAGCTGCCGGGTTTTCGATGCCCGCCGGATACATTCACTTACACGATTTTGATAAGCTCGTATTGTAGATATGGGTTGGAGACTGGGTGCAGGAAGGCCACTAGGAGGAGGTTGTGGGAAGCAAACCACATGTTTCGGAATATGCTCTTTAGAGGTTTTGTTCCTGATGTTGTGACTTACAATGCTTTGATCAATGGTTGTTGCAAGACTTACAGGATAGAGAGGGCGTTGGAGTTGTTTGGGGATATGAAGAAGAGGGATTGTACCCCCAACCGGGTTACTTATGATTCGTTTATTAGATGCTATGCTGCTGTTAATGAGATTGACAAGGCTGTGGATATGTTAAGAAGGATGCAGAATATGAATCATGGATTGGCCACTTCAAGTTCGTATACTCCAATCATTCATGCCCTGTGTGAAGCAGGAAGGGTTACAGAGGCTTGGGGTTTTCTTGTTGAGTTGGTTGATGGAGGGTCAATACCTAGAGAATATACTTACAAATTAGTTTGCAATGCAATGAGTTCAGCTGGAGAGGTCAATTTACTTGATGATGGCCTGCGAAAAAGAATAGAAGATGGTATGCAGAGGAGATATAAGCACATAATGAAGGTGAAGCCAATAATGACTCGCAAAGGGTATGATAGCATGGTGGAAACATGA

Protein Analysis

451

Amino Acids

51.89

Weight (kDa)

9.39

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 173 - 218 4.7e-09 PPR repeat family
PPR_1 PF12854 201 - 233 9.1e-09 PPR repeat
PPR_2 PF13041 208 - 255 3.7e-11 PPR repeat family
PPR_1 PF12854 239 - 257 7.4e-06 PPR repeat
PPR_2 PF13041 268 - 301 1.9e-08 PPR repeat family
PPR_3 PF13812 276 - 334 4.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 283 - 316 2e-11 PPR repeat
PPR_2 PF13041 287 - 335 7.1e-18 PPR repeat family
PPR PF01535 290 - 320 3.4e-09 PPR repeat
PPR_long PF17177 290 - 395 2.5e-08 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 1088
AccII CGCG 2 cut(s) 222, 644
AciI CCGC 4 cut(s) 25, 220, 575, 719
AcoI YGGCCR 2 cut(s) 60, 1071
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 1 cut(s) 961
AfiI CCNNNNNNNGG 6 cut(s) 213, 319, 378, 801, 969, 970
AflIII ACRYGT 2 cut(s) 471, 825
AgsI TTSAA 4 cut(s) 385, 445, 667, 1080
AhlI ACTAGT 1 cut(s) 40
AjiI CACGTC 1 cut(s) 277
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
AluBI AGCT 4 cut(s) 299, 700, 753, 1223
AluI AGCT 4 cut(s) 299, 700, 753, 1223
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 4 cut(s) 145, 303, 402, 770
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 6 cut(s) 60, 289, 447, 792, 1071, 1249
ApeKI GCWGC 4 cut(s) 296, 299, 700, 1004
ApoI RAATTY 1 cut(s) 263
AspA2I CCTAGG 1 cut(s) 541
AspLEI GCGC 2 cut(s) 224, 646
AspS9I GGNCC 3 cut(s) 126, 290, 448
AsuC2I CCSGG 2 cut(s) 705, 971
AsuHPI GGTGA 2 cut(s) 514, 1318
AvaII GGWCC 1 cut(s) 126
AvrII CCTAGG 1 cut(s) 541
BalI TGGCCA 1 cut(s) 1073
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 286, 308, 687, 991
BccI CCATC 6 cut(s) 167, 249, 688, 1157, 1241, 1265
BceAI ACGGC 1 cut(s) 75
BciVI GTATCC 1 cut(s) 718
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 2 cut(s) 705, 971
BcoDI GTCTC 4 cut(s) 145, 303, 402, 770
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 542, 798, 1179
BfmI CTRYAG 1 cut(s) 583
BfuI GTATCC 1 cut(s) 718
BglI GCCNNNNNGGC 1 cut(s) 207
BisI GCNGC 5 cut(s) 297, 300, 576, 701, 1005
BlnI CCTAGG 1 cut(s) 541
BlsI GCNGC 5 cut(s) 298, 301, 577, 702, 1006
Bme1390I CCNGG 2 cut(s) 705, 971
Bme18I GGWCC 1 cut(s) 126
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 3 cut(s) 126, 290, 448
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 705, 971
BmrI ACTGGG 2 cut(s) 370, 789
BmsI GCATC 4 cut(s) 604, 704, 986, 1038
BmuI ACTGGG 2 cut(s) 370, 789
BpmI CTGGAG 2 cut(s) 707, 1245
Bpu10I CCTNAGC 1 cut(s) 314
BpuMI CCSGG 2 cut(s) 705, 971
BsaI GGTCTC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 122, 541
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 767, 797
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 319, 378, 801, 969, 970
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 178, 365, 784
Bse3DI GCAATG 3 cut(s) 601, 1213, 1218
BseDI CCNNGG 2 cut(s) 122, 541
BseGI GGATG 4 cut(s) 159, 594, 619, 1053
BseLI CCNNNNNNNGG 6 cut(s) 213, 319, 378, 801, 969, 970
BseMI GCAATG 3 cut(s) 601, 1213, 1218
BseMII CTCAG 4 cut(s) 111, 150, 259, 328
BseNI ACTGG 3 cut(s) 178, 365, 784
BseRI GAGGAG 2 cut(s) 817, 1297
BseXI GCAGC 4 cut(s) 286, 308, 687, 991
BseYI CCCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 805
Bsh1236I CGCG 2 cut(s) 222, 644
BshFI GGCC 6 cut(s) 62, 291, 449, 794, 1073, 1251
BshNI GGYRCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 6 cut(s) 207, 214, 611, 704, 722, 970
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 6 cut(s) 213, 319, 378, 801, 969, 970
BsmAI GTCTC 4 cut(s) 145, 303, 402, 770
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 6 cut(s) 62, 291, 449, 794, 1073, 1251
Bso31I GGTCTC 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 282, 346
Bsp143I GATC 2 cut(s) 519, 885
Bsp19I CCATGG 1 cut(s) 122
BspACI CCGC 4 cut(s) 25, 220, 575, 719
BspANI GGCC 6 cut(s) 62, 291, 449, 794, 1073, 1251
BspCNI CTCAG 4 cut(s) 110, 149, 258, 327
BspFNI CGCG 2 cut(s) 222, 644
BspLI GGNNCC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 303
BsrDI GCAATG 3 cut(s) 601, 1213, 1218
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 178, 365, 784
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 122, 541
BssMI GATC 2 cut(s) 519, 885
BssNAI GTATAC 1 cut(s) 1089
BssT1I CCWWGG 2 cut(s) 122, 541
Bst1107I GTATAC 1 cut(s) 1089
Bst4CI ACNGT 1 cut(s) 516
Bst6I CTCTTC 3 cut(s) 473, 543, 944
BstC8I GCNNGC 3 cut(s) 208, 719, 1253
BstDEI CTNAG 4 cut(s) 97, 136, 245, 314
BstDSI CCRYGG 1 cut(s) 122
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 4 cut(s) 159, 594, 619, 1053
BstFNI CGCG 2 cut(s) 222, 644
BstHHI GCGC 2 cut(s) 224, 646
BstKTI GATC 2 cut(s) 522, 888
BstMAI GTCTC 4 cut(s) 145, 303, 402, 770
BstMBI GATC 2 cut(s) 519, 885
BstMWI GCNNNNNNNGC 3 cut(s) 207, 575, 697
BstNSI RCATGY 2 cut(s) 475, 829
BstSCI CCNGG 2 cut(s) 703, 969
BstSFI CTRYAG 1 cut(s) 583
BstUI CGCG 2 cut(s) 222, 644
BstV1I GCAGC 4 cut(s) 286, 308, 687, 991
BstZ17I GTATAC 1 cut(s) 1089
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 6 cut(s) 62, 291, 449, 794, 1073, 1251
BtgI CCRYGG 1 cut(s) 122
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 4 cut(s) 159, 594, 619, 1053
Cac8I GCNNGC 3 cut(s) 208, 719, 1253
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 2 cut(s) 224, 646
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 126, 290, 448
Csp6I GTAC 1 cut(s) 960
CviAII CATG 9 cut(s) 64, 123, 412, 472, 826, 1064, 1103, 1342, 1353
CviQI GTAC 1 cut(s) 960
DdeI CTNAG 4 cut(s) 97, 136, 245, 314
DpnI GATC 2 cut(s) 521, 887
DpnII GATC 2 cut(s) 519, 885
DrdI GACNNNNNNGTC 1 cut(s) 416
DseDI GACNNNNNNGTC 1 cut(s) 416
EaeI YGGCCR 2 cut(s) 60, 1071
Eam1104I CTCTTC 3 cut(s) 473, 543, 944
EarI CTCTTC 3 cut(s) 473, 543, 944
Eco130I CCWWGG 2 cut(s) 122, 541
Eco24I GRGCYC 1 cut(s) 346
Eco31I GGTCTC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 126
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 263
EcoT14I CCWWGG 2 cut(s) 122, 541
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 2 cut(s) 122, 541
FaeI CATG 9 cut(s) 67, 126, 415, 475, 829, 1067, 1106, 1345, 1356
FaqI GGGAC 1 cut(s) 166
FatI CATG 9 cut(s) 63, 122, 411, 471, 825, 1063, 1102, 1341, 1352
FauI CCCGC 1 cut(s) 726
FauNDI CATATG 1 cut(s) 259
FbaI TGATCA 1 cut(s) 885
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 5 cut(s) 297, 300, 576, 701, 1005
FokI GGATG 4 cut(s) 146, 601, 626, 1060
FriOI GRGCYC 1 cut(s) 346
Fsp4HI GCNGC 5 cut(s) 297, 300, 576, 701, 1005
FspBI CTAG 4 cut(s) 41, 542, 798, 1179
GlaI GCGC 2 cut(s) 223, 645
GluI GCNGC 5 cut(s) 297, 300, 576, 701, 1005
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 2 cut(s) 707, 1245
HaeIII GGCC 6 cut(s) 62, 291, 449, 794, 1073, 1251
HapII CCGG 6 cut(s) 207, 214, 611, 704, 722, 970
HhaI GCGC 2 cut(s) 224, 646
Hin1II CATG 9 cut(s) 67, 126, 415, 475, 829, 1067, 1106, 1345, 1356
Hin6I GCGC 2 cut(s) 222, 644
HinP1I GCGC 2 cut(s) 222, 644
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 6 cut(s) 134, 168, 436, 983, 1060, 1321
HpaII CCGG 6 cut(s) 207, 214, 611, 704, 722, 970
HphI GGTGA 2 cut(s) 514, 1318
Hpy166II GTNNAC 4 cut(s) 46, 337, 562, 1089
Hpy188I TCNGA 4 cut(s) 21, 133, 465, 834
Hpy188III TCNNGA 3 cut(s) 55, 667, 860
Hpy8I GTNNAC 4 cut(s) 46, 337, 562, 1089
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 6 cut(s) 439, 664, 784, 1038, 1116, 1297
HpyCH4III ACNGT 1 cut(s) 516
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 9 cut(s) 405, 606, 651, 786, 900, 1051, 1206, 1211, 1279
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 575, 697
HpyF3I CTNAG 4 cut(s) 97, 136, 245, 314
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 9 cut(s) 67, 126, 415, 475, 829, 1067, 1106, 1345, 1356
HspAI GCGC 2 cut(s) 222, 644
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 2 cut(s) 519, 885
LmnI GCTCC 4 cut(s) 194, 229, 688, 697
Lsp1109I GCAGC 4 cut(s) 286, 308, 687, 991
LweI GCATC 4 cut(s) 604, 704, 986, 1038
MaeI CTAG 4 cut(s) 41, 542, 798, 1179
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 4 cut(s) 505, 868, 973, 1126
MalI GATC 2 cut(s) 521, 887
MboI GATC 2 cut(s) 519, 885
MboII GAAGA 7 cut(s) 163, 166, 490, 560, 958, 961, 1280
MhlI GDGCHC 2 cut(s) 282, 346
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 4 cut(s) 28, 263, 1196, 1234
MluNI TGGCCA 1 cut(s) 1073
MlyI GAGTC 2 cut(s) 128, 1315
MmeI TCCRAC 3 cut(s) 156, 753, 906
Mox20I TGGCCA 1 cut(s) 1073
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 3 cut(s) 230, 1011, 1040
Msp20I TGGCCA 1 cut(s) 1073
MspA1I CMGCKG 2 cut(s) 299, 1223
MspI CCGG 6 cut(s) 207, 214, 611, 704, 722, 970
MspR9I CCNGG 2 cut(s) 705, 971
MvnI CGCG 2 cut(s) 222, 644
MwoI GCNNNNNNNGC 3 cut(s) 207, 575, 697
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 2 cut(s) 705, 971
NcoI CCATGG 1 cut(s) 122
NdeI CATATG 1 cut(s) 259
NdeII GATC 2 cut(s) 519, 885
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 9 cut(s) 67, 126, 415, 475, 829, 1067, 1106, 1345, 1356
NlaIV GGNNCC 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 868
NspI RCATGY 2 cut(s) 475, 829
PciI ACATGT 2 cut(s) 471, 825
PcsI WCGNNNNNNNCGW 1 cut(s) 515
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 4 cut(s) 168, 436, 983, 1060
PflFI GACNNNGTC 2 cut(s) 137, 514
PkrI GCNGC 5 cut(s) 298, 301, 577, 702, 1006
PleI GAGTC 2 cut(s) 128, 1315
PpsI GAGTC 2 cut(s) 128, 1315
PscI ACATGT 2 cut(s) 471, 825
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 3 cut(s) 126, 290, 448
PsrI GAACNNNNNNTAC 2 cut(s) 14, 46
PstNI CAGNNNCTG 1 cut(s) 299
PsyI GACNNNGTC 2 cut(s) 137, 514
PvuII CAGCTG 2 cut(s) 299, 1223
RsaI GTAC 1 cut(s) 961
RsaNI GTAC 1 cut(s) 960
SaqAI TTAA 3 cut(s) 230, 1011, 1040
SatI GCNGC 5 cut(s) 297, 300, 576, 701, 1005
Sau3AI GATC 2 cut(s) 519, 885
Sau96I GGNCC 3 cut(s) 126, 290, 448
SchI GAGTC 2 cut(s) 128, 1315
ScrFI CCNGG 2 cut(s) 705, 971
SduI GDGCHC 2 cut(s) 282, 346
SfaNI GCATC 4 cut(s) 604, 704, 986, 1038
SfcI CTRYAG 1 cut(s) 583
SinI GGWCC 1 cut(s) 126
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 4 cut(s) 28, 263, 1196, 1234
SsiI CCGC 4 cut(s) 25, 220, 575, 719
SspMI CTAG 4 cut(s) 41, 542, 798, 1179
StyD4I CCNGG 2 cut(s) 703, 969
StyI CCWWGG 2 cut(s) 122, 541
TaaI ACNGT 1 cut(s) 516
TaiI ACGT 1 cut(s) 279
TaqI TCGA 5 cut(s) 348, 366, 476, 518, 712
TasI AATT 4 cut(s) 28, 263, 1196, 1234
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 4 cut(s) 168, 436, 983, 1060
Tru1I TTAA 3 cut(s) 230, 1011, 1040
Tru9I TTAA 3 cut(s) 230, 1011, 1040
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 4 cut(s) 296, 299, 700, 1004
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 9 cut(s) 17, 276, 548, 590, 605, 959, 1073, 1091, 1316
TspGWI ACGGA 1 cut(s) 216
Tth111I GACNNNGTC 2 cut(s) 137, 514
VpaK11BI GGWCC 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 1 cut(s) 263
XceI RCATGY 2 cut(s) 475, 829
XmaJI CCTAGG 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 1088
XspI CTAG 4 cut(s) 41, 542, 798, 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.