Rroxscaffold_5G00363250

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006654
Physical Location & Seq
Forward (+)
44398174 .. 44401907
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363250.1

Sequence Viewer

Length: 1344 bp
ATGAAGCACTCAAAACCTCTGAACCGCAATTACACACCTCTAGTGAACCAAGTCCTGACAGCCATGCTCAAAAACCAACCTTTTGTCTCAGTTTCAGCCACCACAACTCAACCATGGACCTCCGACTCAGTCTCCCAAGTCCTAACCTCCATCCCCAGATTCTTCTTCCAGTCCCCTCGCTCCATAGGTCGCCAGACCGGCTTCCGGCACCGCGCTCCGTTAAAGCAACGCCATCTCAGGCAAGAAACATATGAATTCCACAACGACATGCTCATTCTTGGCCCAGCTGCCCACAGAGACCTCAGCAAGGTCAATCTGGGAGTGGACAAAGCCCTCGATTTTTACTACTGGGTCGAAACCCACTTCGGGTTCAACCACAATGAGCGAACTTGCAGAGACATGGCTGTCGTTTTGGCTAAAGGGAATCGGTTGAAGGCCCTCTGGGATTTTCTGAAAGACATGTCAAAGAGAGGGAGTAGTGAGAGCCCTCTTGTAACGACACAGTCCATCACCTGTTTGATGAAATGCCTAGGAGAAGAGGGATTGGTTAATGAAGCATTGGCGGCTTTCTATAGGATGAAGCAGTTTCATTGCAGACCGGATGTGTATGCTTACAACACTATTATTTACGCGCTTTGCAGAGTTGGGAATTTCAAGAAGGCCCGGTTTTTGCTGGAGCAGATGGAGCTGCCGGGTTTTAGATGCCCGCCGGATACATTCACTTACACGATTTTGATAAGCTCTTATTGTAGATATGGGTTGGAGACTGGGTGCAGGAAGGCCACTAGGAGGAGGTTGTGGGAAGCAAACCACATGTTTCGGAATATGCTCTTTAGAGGTTTTGTTCCTGATGTTGTTACTTACAATGCTTTGATCAATGGTTGTTGCAAGACTTACAGGATAGAGAGGGCATTGGAGTTGTTTGAGGATATGAAGAAGAAGGATTGTACCCCCAACCGGGTTACTTATGATTCGTTTATTAGATACTATGCTGCTGTTAATGAGATTGACAAGGGTGTGGATATGTTAAGAAGGATGCAGAATATGAATCATGGATTGGCCACTTCAAGTTCGTATACTCCAATCATTCATGCCATGTGTGAAGCAGGAAGGGTTACAGAGGCTTGGGGTTTTCTTGTTGAGTTGGTTGATGGAGGGTCAATACCTAGAGAATATACTTACAAATTAGTTTGCAATGCAATGAGTTCAGCTGGAGAGGTCAATTTACTTGATGATAGCCTGCGAAAAAGAATAGAAGATGGTATGGAGAGGAGATATAAGCACATAATGAAGGTGAAGCCAATAATGACTCGCAAAGGGTATGATAGCATGGTGGAAACATGA

Protein Analysis

447

Amino Acids

51.73

Weight (kDa)

9.36

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 214 6.9e-09 PPR repeat family
PPR_1 PF12854 197 - 229 6.3e-09 PPR repeat
PPR_2 PF13041 200 - 251 4.9e-13 PPR repeat family
PPR_1 PF12854 235 - 253 7.4e-06 PPR repeat
PPR_2 PF13041 264 - 297 1.9e-08 PPR repeat family
PPR_3 PF13812 272 - 330 6e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 279 - 312 2.9e-12 PPR repeat
PPR_2 PF13041 283 - 328 1e-17 PPR repeat family
PPR PF01535 286 - 316 1.2e-09 PPR repeat
PPR_long PF17177 286 - 396 3.6e-08 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 1076
AccII CGCG 2 cut(s) 213, 632
AciI CCGC 4 cut(s) 25, 211, 563, 707
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 2 cut(s) 254, 649
AfaI GTAC 1 cut(s) 949
AfiI CCNNNNNNNGG 6 cut(s) 204, 307, 366, 789, 957, 958
AflIII ACRYGT 2 cut(s) 459, 813
AgsI TTSAA 4 cut(s) 373, 433, 655, 1068
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
AluBI AGCT 4 cut(s) 287, 688, 741, 1211
AluI AGCT 4 cut(s) 287, 688, 741, 1211
Alw26I GTCTC 5 cut(s) 91, 136, 291, 390, 758
AoxI GGCC 5 cut(s) 280, 435, 660, 780, 1059
ApeKI GCWGC 3 cut(s) 287, 688, 992
ApoI RAATTY 2 cut(s) 254, 649
AspA2I CCTAGG 1 cut(s) 529
AspLEI GCGC 2 cut(s) 215, 634
AspS9I GGNCC 4 cut(s) 117, 281, 436, 661
AsuC2I CCSGG 3 cut(s) 664, 693, 959
AsuHPI GGTGA 2 cut(s) 502, 1306
AvaII GGWCC 1 cut(s) 117
AvrII CCTAGG 1 cut(s) 529
BalI TGGCCA 1 cut(s) 1061
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 488
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 3 cut(s) 274, 675, 979
BccI CCATC 6 cut(s) 158, 240, 515, 676, 1145, 1253
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 1 cut(s) 873
BcnI CCSGG 3 cut(s) 664, 693, 959
BcoDI GTCTC 5 cut(s) 91, 136, 291, 390, 758
BfaI CTAG 4 cut(s) 41, 530, 786, 1167
BfmI CTRYAG 1 cut(s) 571
BfuI GTATCC 1 cut(s) 706
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 4 cut(s) 288, 564, 689, 993
BlnI CCTAGG 1 cut(s) 529
BlsI GCNGC 4 cut(s) 289, 565, 690, 994
Bme1390I CCNGG 3 cut(s) 664, 693, 959
Bme18I GGWCC 1 cut(s) 117
BmgT120I GGNCC 4 cut(s) 117, 281, 436, 661
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 3 cut(s) 664, 693, 959
BmrI ACTGGG 2 cut(s) 358, 777
BmsI GCATC 2 cut(s) 692, 1026
BmuI ACTGGG 2 cut(s) 358, 777
BpmI CTGGAG 2 cut(s) 695, 1233
Bpu10I CCTNAGC 1 cut(s) 302
BpuMI CCSGG 3 cut(s) 664, 693, 959
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 113, 529
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 755, 785
Bsc4I CCNNNNNNNGG 6 cut(s) 204, 307, 366, 789, 957, 958
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 169, 353, 772
Bse3DI GCAATG 3 cut(s) 589, 1201, 1206
BseDI CCNNGG 2 cut(s) 113, 529
BseGI GGATG 4 cut(s) 150, 582, 607, 1041
BseLI CCNNNNNNNGG 6 cut(s) 204, 307, 366, 789, 957, 958
BseMI GCAATG 3 cut(s) 589, 1201, 1206
BseMII CTCAG 4 cut(s) 102, 141, 250, 316
BseNI ACTGG 3 cut(s) 169, 353, 772
BseRI GAGGAG 2 cut(s) 805, 1285
BseXI GCAGC 3 cut(s) 274, 675, 979
BseYI CCCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 793
Bsh1236I CGCG 2 cut(s) 213, 632
BshFI GGCC 5 cut(s) 282, 437, 662, 782, 1061
BshNI GGYRCC 1 cut(s) 207
BsiSI CCGG 7 cut(s) 198, 205, 599, 664, 692, 710, 958
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 6 cut(s) 204, 307, 366, 789, 957, 958
BsmAI GTCTC 5 cut(s) 91, 136, 291, 390, 758
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 5 cut(s) 282, 437, 662, 782, 1061
Bso31I GGTCTC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 4 cut(s) 25, 211, 563, 707
BspANI GGCC 5 cut(s) 282, 437, 662, 782, 1061
BspCNI CTCAG 4 cut(s) 101, 140, 249, 315
BspFNI CGCG 2 cut(s) 213, 632
BspLI GGNNCC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 3 cut(s) 589, 1201, 1206
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 169, 353, 772
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 113, 529
BssMI GATC 1 cut(s) 873
BssNAI GTATAC 1 cut(s) 1077
BssT1I CCWWGG 2 cut(s) 113, 529
Bst1107I GTATAC 1 cut(s) 1077
Bst4CI ACNGT 1 cut(s) 504
Bst6I CTCTTC 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 707, 1241
BstDEI CTNAG 4 cut(s) 88, 127, 236, 302
BstDSI CCRYGG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 4 cut(s) 150, 582, 607, 1041
BstFNI CGCG 2 cut(s) 213, 632
BstHHI GCGC 2 cut(s) 215, 634
BstKTI GATC 1 cut(s) 876
BstMAI GTCTC 5 cut(s) 91, 136, 291, 390, 758
BstMBI GATC 1 cut(s) 873
BstMWI GCNNNNNNNGC 3 cut(s) 198, 563, 685
BstNSI RCATGY 3 cut(s) 271, 463, 817
BstSCI CCNGG 3 cut(s) 662, 691, 957
BstSFI CTRYAG 1 cut(s) 571
BstUI CGCG 2 cut(s) 213, 632
BstV1I GCAGC 3 cut(s) 274, 675, 979
BstZ17I GTATAC 1 cut(s) 1077
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 5 cut(s) 282, 437, 662, 782, 1061
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 4 cut(s) 150, 582, 607, 1041
Cac8I GCNNGC 2 cut(s) 707, 1241
CfoI GCGC 2 cut(s) 215, 634
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 4 cut(s) 117, 281, 436, 661
Csp6I GTAC 1 cut(s) 948
CviQI GTAC 1 cut(s) 948
DdeI CTNAG 4 cut(s) 88, 127, 236, 302
DpnI GATC 1 cut(s) 875
DpnII GATC 1 cut(s) 873
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 1 cut(s) 1059
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 113, 529
Eco24I GRGCYC 1 cut(s) 488
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 117
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 254
EcoT14I CCWWGG 2 cut(s) 113, 529
EcoT38I GRGCYC 1 cut(s) 488
ErhI CCWWGG 2 cut(s) 113, 529
FaqI GGGAC 1 cut(s) 157
FauI CCCGC 1 cut(s) 714
FauNDI CATATG 1 cut(s) 250
FbaI TGATCA 1 cut(s) 873
FblI GTMKAC 1 cut(s) 1076
Fnu4HI GCNGC 4 cut(s) 288, 564, 689, 993
FokI GGATG 4 cut(s) 137, 589, 614, 1048
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 288, 564, 689, 993
FspBI CTAG 4 cut(s) 41, 530, 786, 1167
GlaI GCGC 2 cut(s) 214, 633
GluI GCNGC 4 cut(s) 288, 564, 689, 993
GsaI CCCAGC 1 cut(s) 287
GsuI CTGGAG 2 cut(s) 695, 1233
HaeIII GGCC 5 cut(s) 282, 437, 662, 782, 1061
HapII CCGG 7 cut(s) 198, 205, 599, 664, 692, 710, 958
HhaI GCGC 2 cut(s) 215, 634
Hin6I GCGC 2 cut(s) 213, 632
HinP1I GCGC 2 cut(s) 213, 632
HinfI GANTC 6 cut(s) 125, 159, 424, 971, 1048, 1309
HpaII CCGG 7 cut(s) 198, 205, 599, 664, 692, 710, 958
HphI GGTGA 2 cut(s) 502, 1306
Hpy166II GTNNAC 3 cut(s) 46, 325, 1077
Hpy188I TCNGA 4 cut(s) 21, 124, 453, 822
Hpy188III TCNNGA 3 cut(s) 55, 655, 848
Hpy8I GTNNAC 3 cut(s) 46, 325, 1077
HpyAV CCTTC 7 cut(s) 427, 652, 772, 934, 1026, 1104, 1285
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4V TGCA 8 cut(s) 393, 594, 639, 774, 888, 1039, 1194, 1199
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 563, 685
HpyF3I CTNAG 4 cut(s) 88, 127, 236, 302
HspAI GCGC 2 cut(s) 213, 632
Ksp22I TGATCA 1 cut(s) 873
Kzo9I GATC 1 cut(s) 873
LmnI GCTCC 4 cut(s) 185, 220, 676, 685
Lsp1109I GCAGC 3 cut(s) 274, 675, 979
LweI GCATC 2 cut(s) 692, 1026
MaeI CTAG 4 cut(s) 41, 530, 786, 1167
MaeIII GTNAC 4 cut(s) 493, 856, 961, 1114
MalI GATC 1 cut(s) 875
MboI GATC 1 cut(s) 873
MboII GAAGA 6 cut(s) 154, 157, 548, 946, 949, 1268
MhlI GDGCHC 1 cut(s) 488
MlsI TGGCCA 1 cut(s) 1061
MluCI AATT 5 cut(s) 28, 254, 649, 1184, 1222
MluNI TGGCCA 1 cut(s) 1061
MlyI GAGTC 2 cut(s) 119, 1303
MmeI TCCRAC 2 cut(s) 147, 741
Mox20I TGGCCA 1 cut(s) 1061
MscI TGGCCA 1 cut(s) 1061
MseI TTAA 4 cut(s) 221, 549, 999, 1028
Msp20I TGGCCA 1 cut(s) 1061
MspA1I CMGCKG 2 cut(s) 287, 1211
MspI CCGG 7 cut(s) 198, 205, 599, 664, 692, 710, 958
MspR9I CCNGG 3 cut(s) 664, 693, 959
MvnI CGCG 2 cut(s) 213, 632
MwoI GCNNNNNNNGC 3 cut(s) 198, 563, 685
NciI CCSGG 3 cut(s) 664, 693, 959
NcoI CCATGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 250
NdeII GATC 1 cut(s) 873
NlaIV GGNNCC 1 cut(s) 209
NspI RCATGY 3 cut(s) 271, 463, 817
PciI ACATGT 2 cut(s) 459, 813
PfeI GAWTC 4 cut(s) 159, 424, 971, 1048
PflFI GACNNNGTC 2 cut(s) 128, 502
PkrI GCNGC 4 cut(s) 289, 565, 690, 994
PleI GAGTC 2 cut(s) 119, 1303
PpsI GAGTC 2 cut(s) 119, 1303
PscI ACATGT 2 cut(s) 459, 813
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 4 cut(s) 117, 281, 436, 661
PsrI GAACNNNNNNTAC 2 cut(s) 14, 46
PsyI GACNNNGTC 2 cut(s) 128, 502
PvuII CAGCTG 2 cut(s) 287, 1211
RsaI GTAC 1 cut(s) 949
RsaNI GTAC 1 cut(s) 948
SaqAI TTAA 4 cut(s) 221, 549, 999, 1028
SatI GCNGC 4 cut(s) 288, 564, 689, 993
Sau3AI GATC 1 cut(s) 873
Sau96I GGNCC 4 cut(s) 117, 281, 436, 661
SchI GAGTC 2 cut(s) 119, 1303
ScrFI CCNGG 3 cut(s) 664, 693, 959
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 2 cut(s) 692, 1026
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 1 cut(s) 117
Sse9I AATT 5 cut(s) 28, 254, 649, 1184, 1222
SsiI CCGC 4 cut(s) 25, 211, 563, 707
SspMI CTAG 4 cut(s) 41, 530, 786, 1167
StyD4I CCNGG 3 cut(s) 662, 691, 957
StyI CCWWGG 2 cut(s) 113, 529
TaaI ACNGT 1 cut(s) 504
TaqI TCGA 2 cut(s) 336, 354
TasI AATT 5 cut(s) 28, 254, 649, 1184, 1222
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 4 cut(s) 159, 424, 971, 1048
Tru1I TTAA 4 cut(s) 221, 549, 999, 1028
Tru9I TTAA 4 cut(s) 221, 549, 999, 1028
TseI GCWGC 3 cut(s) 287, 688, 992
TspGWI ACGGA 1 cut(s) 207
Tth111I GACNNNGTC 2 cut(s) 128, 502
VpaK11BI GGWCC 1 cut(s) 117
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 254, 649
XceI RCATGY 3 cut(s) 271, 463, 817
XmaJI CCTAGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 1076
XspI CTAG 4 cut(s) 41, 530, 786, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.