AT2G06845

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005302
Physical Location & Seq
Forward (+)
2753443 .. 2756008
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G06845.1

Sequence Viewer

Length: 1227 bp
ATGGTTCCTTTAGATTGGTCTGATAGTTACCTTTGTCGAGTTCAGATGTTAAGTTTATTTTGGACCCTACCCGAGATCCTCTTCCCCACCTATTGCCTTTTGCCCTTTACAGTGAAGAAGATTGACATTATGATACCGCCTCTACGGAGTGGGTATTACCAAAGATTTTTCACCTCTCTACCTACCCATTCACCAATTATCGAGTTCAGTCATGTGTTAATATATTGCTTGGATAATTTGCAATCCTCTGTTTGTTTGGGAAAGTACGGCATTATGACCATGTCTTCAAGAGGTGGTTACCGTCTCTTTCTCAACCTCGTTAACCCGTCAGCCTCCTCCGTGGAGCACCTTTTCAAAAGCTCAGATGCATTGTTTGCAAGAGCTGCTTATGATCACTTGGTGGTCGAGGATTTCGCGAAGCTCGCCTTCATGCTTATATATTATACATTTAAACTTTCGGATGCGACTGGTCAACAACTCTTGGATAGACATATTGCCATTGATGCTTCTTGCTGTAGGTGTGGAGAGCCGGAGACTATTCTTCACATGTTGTTTCACTGCCAGTTTGCGCAGTTAGTGTGGAGTCCGACACCGTTTGCAAGACACTGGGCAAGTGTTGGAGCAGGGAGTGTTCGAGAAGGCTTGATCACCGGTTGTAAGCTTGCATGTCTACCGCCGACTGGAATTGCTTCGGGGCCTTTAGCTCCTTGGATCTGCTGGAGTCTATGGAAATCAAGGAACCAAAAAGTGTTTAGTACCAGGTTCTTCACCCCAGAAGAAACTTTGTTAAAGGCTATCACGAATGCTAAGGAGTGGCTGGCAGCTCAAGGCAAATCACCTGACGGATCATGGCGCAATGATCTAAAGGCAGCTGGCTTAGGGTGGACCTTCACCAATCCGGCTGGTCTTACCTCTCATCATTCTGCTCTTTGCAAGAATGTATCATCACCTTTCATGGCGGAATCTTTGGCCTGTGGAGCTGTTGTCTTGGAAGCGGTCAGGGCAGGGGCGGATAGCTTCCTCCTAGAATCTGACTATCAACAATTGGTCGTCGCAATCAATGCCCGATTAGTTTTATTGGAAGTTCATGGAATCATTTCATATATCTTCCTTAGCATTGGTAGATTTTCTCACTTCTTATGTCGCTTCATTCCTCTGTCAACTAATGTTGTGGCTGATTCACTTGCTAAACAATGTCTTAGTTTGTACGGACAAAACATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

408

Amino Acids

45.4

Weight (kDa)

8.11

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 155 - 194 1.4e-07 zinc-binding in reverse transcriptase
RVT_3 PF13456 280 - 399 7.8e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 570
AccI GTMKAC 1 cut(s) 670
AccII CGCG 1 cut(s) 416
AciI CCGC 5 cut(s) 137, 674, 959, 995, 1010
AclWI GGATC 3 cut(s) 70, 719, 853
AdeI CACNNNGTG 1 cut(s) 400
AfaI GTAC 3 cut(s) 266, 757, 1208
AfiI CCNNNNNNNGG 1 cut(s) 680
AflIII ACRYGT 1 cut(s) 546
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 2 cut(s) 288, 355
AjnI CCWGG 1 cut(s) 758
AluBI AGCT 9 cut(s) 360, 383, 421, 661, 704, 824, 872, 980, 1017
AluI AGCT 9 cut(s) 360, 383, 421, 661, 704, 824, 872, 980, 1017
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 308, 527
AlwI GGATC 3 cut(s) 70, 719, 853
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 2 cut(s) 695, 969
ApeKI GCWGC 3 cut(s) 383, 821, 869
ArsI GACNNNNNNTTYG 1 cut(s) 1203
AsiGI ACCGGT 1 cut(s) 650
Asp700I GAANNNNTTC 2 cut(s) 688, 1096
AspLEI GCGC 2 cut(s) 571, 855
AspS9I GGNCC 3 cut(s) 63, 695, 885
AsuHPI GGTGA 7 cut(s) 163, 183, 640, 760, 828, 883, 939
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 2 cut(s) 63, 885
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 370, 833, 881
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 760
BclI TGATCA 2 cut(s) 391, 645
BcoDI GTCTC 2 cut(s) 308, 527
BfaI CTAG 1 cut(s) 1025
BfmI CTRYAG 1 cut(s) 514
BisI GCNGC 3 cut(s) 384, 822, 870
BlsI GCNGC 3 cut(s) 385, 823, 871
Bme1390I CCNGG 1 cut(s) 760
Bme18I GGWCC 2 cut(s) 63, 885
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 3 cut(s) 63, 695, 885
BmiI GGNNCC 4 cut(s) 6, 65, 696, 740
BmrFI CCNGG 1 cut(s) 760
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 3 cut(s) 355, 451, 493
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 739
Bpu10I CCTNAGC 3 cut(s) 807, 877, 1112
BpuEI CTTGAG 1 cut(s) 810
BsaJI CCNNGG 2 cut(s) 339, 707
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 1 cut(s) 680
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 4 cut(s) 472, 562, 611, 685
Bse3DI GCAATG 1 cut(s) 862
BseBI CCWGG 1 cut(s) 760
BseDI CCNNGG 2 cut(s) 339, 707
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMI GCAATG 1 cut(s) 862
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 4 cut(s) 472, 562, 611, 685
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 3 cut(s) 370, 833, 881
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 697, 971
BshTI ACCGGT 1 cut(s) 650
BsiHKAI GWGCWC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 3 cut(s) 530, 651, 899
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 2 cut(s) 308, 527
BsmBI CGTCTC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 2 cut(s) 697, 971
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 6 cut(s) 75, 391, 645, 711, 845, 859
Bsp68I TCGCGA 1 cut(s) 416
BspACI CCGC 5 cut(s) 137, 674, 959, 995, 1010
BspANI GGCC 2 cut(s) 697, 971
BspCNI CTCAG 1 cut(s) 374
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 4 cut(s) 6, 65, 696, 740
BspPI GGATC 3 cut(s) 70, 719, 853
BsrDI GCAATG 1 cut(s) 862
BsrFI RCCGGY 1 cut(s) 650
BsrI ACTGG 4 cut(s) 472, 562, 611, 685
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 2 cut(s) 339, 707
BssMI GATC 6 cut(s) 75, 391, 645, 711, 845, 859
BssT1I CCWWGG 1 cut(s) 707
Bst2UI CCWGG 1 cut(s) 760
Bst4CI ACNGT 3 cut(s) 112, 302, 594
Bst6I CTCTTC 1 cut(s) 86
BstAPI GCANNNNNTGC 3 cut(s) 374, 383, 1061
BstC8I GCNNGC 4 cut(s) 423, 663, 819, 874
BstDEI CTNAG 5 cut(s) 361, 807, 877, 1112, 1199
BstDSI CCRYGG 1 cut(s) 339
BstEII GGTNACC 1 cut(s) 296
BstF5I GGATG 1 cut(s) 466
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 2 cut(s) 571, 855
BstKTI GATC 6 cut(s) 78, 394, 648, 714, 848, 862
BstMAI GTCTC 2 cut(s) 308, 527
BstMBI GATC 6 cut(s) 75, 391, 645, 711, 845, 859
BstMWI GCNNNNNNNGC 7 cut(s) 374, 383, 422, 503, 977, 1001, 1061
BstNI CCWGG 1 cut(s) 760
BstNSI RCATGY 2 cut(s) 550, 669
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 758
BstSFI CTRYAG 1 cut(s) 514
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 370, 833, 881
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 75, 711
BstYI RGATCY 2 cut(s) 75, 711
BsuRI GGCC 2 cut(s) 697, 971
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 466
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 3 cut(s) 117, 556, 604
BtuMI TCGCGA 1 cut(s) 416
Cac8I GCNNGC 4 cut(s) 423, 663, 819, 874
CfoI GCGC 2 cut(s) 571, 855
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 3 cut(s) 63, 695, 885
CsiI ACCWGGT 1 cut(s) 758
Csp6I GTAC 3 cut(s) 265, 756, 1207
CspAI ACCGGT 1 cut(s) 650
CviAII CATG 8 cut(s) 212, 280, 430, 547, 666, 849, 955, 1088
CviQI GTAC 3 cut(s) 265, 756, 1207
DdeI CTNAG 5 cut(s) 361, 807, 877, 1112, 1199
DpnI GATC 6 cut(s) 77, 393, 647, 713, 847, 861
DpnII GATC 6 cut(s) 75, 391, 645, 711, 845, 859
DraI TTTAAA 1 cut(s) 451
DraIII CACNNNGTG 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 2 cut(s) 974, 1025
Eco130I CCWWGG 1 cut(s) 707
Eco47I GGWCC 2 cut(s) 63, 885
Eco88I CYCGRG 1 cut(s) 71
Eco91I GGTNACC 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 695
EcoO65I GGTNACC 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 758
EcoT14I CCWWGG 1 cut(s) 707
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 707
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 8 cut(s) 215, 283, 433, 550, 669, 852, 958, 1091
FalI AAGNNNNNCTT 4 cut(s) 370, 402, 410, 442
FatI CATG 8 cut(s) 211, 279, 429, 546, 665, 848, 954, 1087
FbaI TGATCA 2 cut(s) 391, 645
FblI GTMKAC 1 cut(s) 670
Fnu4HI GCNGC 3 cut(s) 384, 822, 870
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 3 cut(s) 384, 822, 870
FspBI CTAG 1 cut(s) 1025
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 2 cut(s) 570, 854
GluI GCNGC 3 cut(s) 384, 822, 870
GsuI CTGGAG 1 cut(s) 739
HaeIII GGCC 2 cut(s) 697, 971
HapII CCGG 3 cut(s) 530, 651, 899
HhaI GCGC 2 cut(s) 571, 855
Hin1II CATG 8 cut(s) 215, 283, 433, 550, 669, 852, 958, 1091
Hin6I GCGC 2 cut(s) 569, 853
HinP1I GCGC 2 cut(s) 569, 853
HincII GTYRAC 3 cut(s) 322, 473, 1161
HindII GTYRAC 3 cut(s) 322, 473, 1161
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 6 cut(s) 583, 721, 962, 1028, 1092, 1178
HpaI GTTAAC 1 cut(s) 322
HpaII CCGG 3 cut(s) 530, 651, 899
HphI GGTGA 7 cut(s) 163, 183, 640, 760, 828, 883, 939
Hpy166II GTNNAC 5 cut(s) 322, 473, 671, 885, 1161
Hpy188I TCNGA 6 cut(s) 22, 45, 364, 460, 588, 1033
Hpy188III TCNNGA 4 cut(s) 288, 415, 635, 799
Hpy8I GTNNAC 5 cut(s) 322, 473, 671, 885, 1161
Hpy99I CGWCG 1 cut(s) 1055
HpyAV CCTTC 3 cut(s) 436, 632, 898
HpyCH4III ACNGT 3 cut(s) 112, 302, 594
HpyCH4V TGCA 6 cut(s) 241, 368, 377, 599, 665, 933
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 383, 422, 503, 977, 1001, 1061
HpyF3I CTNAG 5 cut(s) 361, 807, 877, 1112, 1199
Hsp92II CATG 8 cut(s) 215, 283, 433, 550, 669, 852, 958, 1091
HspAI GCGC 2 cut(s) 569, 853
Ksp22I TGATCA 2 cut(s) 391, 645
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 6 cut(s) 75, 391, 645, 711, 845, 859
LmnI GCTCC 4 cut(s) 343, 620, 709, 977
Lsp1109I GCAGC 3 cut(s) 370, 833, 881
LweI GCATC 3 cut(s) 355, 451, 493
MabI ACCWGGT 1 cut(s) 758
MaeI CTAG 1 cut(s) 1025
MaeIII GTNAC 2 cut(s) 26, 296
MalI GATC 6 cut(s) 77, 393, 647, 713, 847, 861
MboI GATC 6 cut(s) 75, 391, 645, 711, 845, 859
MboII GAAGA 8 cut(s) 73, 127, 130, 276, 533, 757, 788, 1099
MfeI CAATTG 1 cut(s) 1043
MflI RGATCY 2 cut(s) 75, 711
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 4 cut(s) 195, 235, 684, 1043
MlyI GAGTC 2 cut(s) 592, 730
MmeI TCCRAC 2 cut(s) 598, 611
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 2 cut(s) 688, 1096
MseI TTAA 5 cut(s) 50, 218, 321, 450, 788
MspA1I CMGCKG 1 cut(s) 872
MspI CCGG 3 cut(s) 530, 651, 899
MspR9I CCNGG 1 cut(s) 760
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 1 cut(s) 760
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 7 cut(s) 374, 383, 422, 503, 977, 1001, 1061
NdeII GATC 6 cut(s) 75, 391, 645, 711, 845, 859
NlaIII CATG 8 cut(s) 215, 283, 433, 550, 669, 852, 958, 1091
NlaIV GGNNCC 4 cut(s) 6, 65, 696, 740
NruI TCGCGA 1 cut(s) 416
NsbI TGCGCA 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 2 cut(s) 550, 669
PciI ACATGT 1 cut(s) 546
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 2 cut(s) 688, 1096
PfeI GAWTC 4 cut(s) 962, 1028, 1092, 1178
PflFI GACNNNGTC 1 cut(s) 280
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 3 cut(s) 385, 823, 871
PleI GAGTC 2 cut(s) 591, 729
PpsI GAGTC 2 cut(s) 591, 729
PscI ACATGT 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 758
PspEI GGTNACC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 758
PspN4I GGNNCC 4 cut(s) 6, 65, 696, 740
PspPI GGNCC 3 cut(s) 63, 695, 885
PsuI RGATCY 2 cut(s) 75, 711
PsyI GACNNNGTC 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 872
RruI TCGCGA 1 cut(s) 416
RsaI GTAC 3 cut(s) 266, 757, 1208
RsaNI GTAC 3 cut(s) 265, 756, 1207
SaqAI TTAA 5 cut(s) 50, 218, 321, 450, 788
SatI GCNGC 3 cut(s) 384, 822, 870
Sau3AI GATC 6 cut(s) 75, 391, 645, 711, 845, 859
Sau96I GGNCC 3 cut(s) 63, 695, 885
SchI GAGTC 2 cut(s) 592, 730
ScrFI CCNGG 1 cut(s) 760
SduI GDGCHC 1 cut(s) 348
SexAI ACCWGGT 1 cut(s) 758
SfaNI GCATC 3 cut(s) 355, 451, 493
SfcI CTRYAG 1 cut(s) 514
SinI GGWCC 2 cut(s) 63, 885
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 4 cut(s) 195, 235, 684, 1043
SsiI CCGC 5 cut(s) 137, 674, 959, 995, 1010
SspMI CTAG 1 cut(s) 1025
StyD4I CCNGG 1 cut(s) 758
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 3 cut(s) 112, 302, 594
TaqI TCGA 4 cut(s) 37, 201, 405, 634
TasI AATT 4 cut(s) 195, 235, 684, 1043
TfiI GAWTC 4 cut(s) 962, 1028, 1092, 1178
Tru1I TTAA 5 cut(s) 50, 218, 321, 450, 788
Tru9I TTAA 5 cut(s) 50, 218, 321, 450, 788
TscAI CASTG 3 cut(s) 117, 563, 611
TseI GCWGC 3 cut(s) 383, 821, 869
TspDTI ATGAA 5 cut(s) 418, 943, 1076, 1089, 1138
TspGWI ACGGA 4 cut(s) 160, 328, 858, 1224
TspRI CASTG 3 cut(s) 117, 563, 611
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 63, 885
XceI RCATGY 2 cut(s) 550, 669
XmiI GTMKAC 1 cut(s) 670
XmnI GAANNNNTTC 2 cut(s) 688, 1096
XspI CTAG 1 cut(s) 1025
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.