AT5G49250

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005302
Physical Location & Seq
Forward (+)
19965343 .. 19965945
603 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49250.1

Sequence Viewer

Length: 603 bp
ATGCTTAGCCCATTACTTTCTTTGTGTCGATCTCCTCTGTTCTATGGTCATGGACTCCATGTGAAAGAGACATCAACATTGACGGTTTGGGTTTTCATTTACCTTATGCGAACCTTGGTTTTAGCTACCTTTAAGGCTTTATGGTATCCTTATAAAGAATTGAAGATTGTTGTGCTAAGTTCCTTGAATCGTTCTTATTGTTGTGGCATTTGTGGTATTGGATTATTCTTTTTCGTTGCTCATTTGCCACTAAGCAGCTCACTTTTCGTCTTCAAACACTGTACCCTCTTTATCATCTCCATCTTCCAGACCATATGCTTCATAGTCTTTGCCGCTATTGACCGGAGAGATCCTCATTCGAGTTGGTTTCTAAAGCCACCCTCACCTGACTATCGCACCATAGTCTCCCGAATCCTTGGTATCGTGTTACTGTGGGTAATGAAGCTTGCTCCGGCGAGATTCATATCATTGATCCGTTTGTCAATGTCACTATATCCTTCGACTGTGACTAATCCGTCGTCCTTCGAACTCTTCGAGGATGATCTCTCGATTTACCGTGACCTCTCATGGTGCATTGCGCTTCCTTGCCTTTTCCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

200

Amino Acids

22.99

Weight (kDa)

9.19

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
AasI GACNNNNNNGTC 1 cut(s) 514
AciI CCGC 1 cut(s) 333
AclWI GGATC 2 cut(s) 344, 466
AfaI GTAC 1 cut(s) 283
AgsI TTSAA 3 cut(s) 163, 187, 274
AluBI AGCT 3 cut(s) 125, 258, 445
AluI AGCT 3 cut(s) 125, 258, 445
Alw26I GTCTC 2 cut(s) 62, 409
AlwI GGATC 2 cut(s) 344, 466
ApeKI GCWGC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 580
AsuHPI GGTGA 1 cut(s) 375
AsuII TTCGAA 1 cut(s) 525
BbsI GAAGAC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 267
BccI CCATC 1 cut(s) 308
BciVI GTATCC 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 62, 409
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 2 cut(s) 256, 333
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 2 cut(s) 257, 334
BpiI GAAGAC 1 cut(s) 262
BplI GAGNNNNNCTC 2 cut(s) 337, 369
Bpu1102I GCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 114, 415
BsaWI WCCGGW 1 cut(s) 342
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 463
BseDI CCNNGG 2 cut(s) 114, 415
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 1 cut(s) 463
BseMI GCAATG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 267
BsiSI CCGG 2 cut(s) 343, 452
BsmAI GTCTC 2 cut(s) 62, 409
Bsp119I TTCGAA 1 cut(s) 525
Bsp143I GATC 4 cut(s) 29, 349, 471, 541
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 1 cut(s) 333
BspPI GGATC 2 cut(s) 344, 466
BspT104I TTCGAA 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 573
BssECI CCNNGG 2 cut(s) 114, 415
BssMI GATC 4 cut(s) 29, 349, 471, 541
BssT1I CCWWGG 2 cut(s) 114, 415
Bst4CI ACNGT 5 cut(s) 85, 281, 432, 505, 557
Bst6I CTCTTC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 525
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 5, 176, 251
BstF5I GGATG 1 cut(s) 544
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 4 cut(s) 32, 352, 474, 544
BstMAI GTCTC 2 cut(s) 62, 409
BstMBI GATC 4 cut(s) 29, 349, 471, 541
BstV1I GCAGC 1 cut(s) 267
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuI GTATCC 1 cut(s) 156
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 580
Csp6I GTAC 1 cut(s) 282
CviAII CATG 3 cut(s) 50, 59, 567
CviJI RGCY 6 cut(s) 9, 125, 137, 258, 376, 445
CviKI_1 RGCY 6 cut(s) 9, 125, 137, 258, 376, 445
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 3 cut(s) 5, 176, 251
DpnI GATC 4 cut(s) 31, 351, 473, 543
DpnII GATC 4 cut(s) 29, 349, 471, 541
DrdI GACNNNNNNGTC 1 cut(s) 514
DseDI GACNNNNNNGTC 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
Eco130I CCWWGG 2 cut(s) 114, 415
EcoT14I CCWWGG 2 cut(s) 114, 415
ErhI CCWWGG 2 cut(s) 114, 415
FaeI CATG 3 cut(s) 53, 62, 570
FatI CATG 3 cut(s) 49, 58, 566
FauNDI CATATG 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 256, 333
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 2 cut(s) 256, 333
GlaI GCGC 1 cut(s) 579
GluI GCNGC 2 cut(s) 256, 333
HapII CCGG 2 cut(s) 343, 452
HhaI GCGC 1 cut(s) 580
Hin1II CATG 3 cut(s) 53, 62, 570
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 4 cut(s) 54, 187, 411, 459
HpaII CCGG 2 cut(s) 343, 452
HphI GGTGA 1 cut(s) 375
Hpy188III TCNNGA 3 cut(s) 307, 408, 547
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 2 cut(s) 507, 532
HpyCH4III ACNGT 5 cut(s) 85, 281, 432, 505, 557
HpyCH4V TGCA 1 cut(s) 573
HpyF3I CTNAG 3 cut(s) 5, 176, 251
Hsp92II CATG 3 cut(s) 53, 62, 570
HspAI GCGC 1 cut(s) 578
Kzo9I GATC 4 cut(s) 29, 349, 471, 541
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 4 cut(s) 320, 356, 399, 465
Lsp1109I GCAGC 1 cut(s) 267
MaeIII GTNAC 4 cut(s) 426, 486, 505, 557
MalI GATC 4 cut(s) 31, 351, 473, 543
MboI GATC 4 cut(s) 29, 349, 471, 541
MboII GAAGA 4 cut(s) 175, 262, 295, 523
MflI RGATCY 1 cut(s) 349
MluCI AATT 1 cut(s) 158
MlyI GAGTC 1 cut(s) 48
MnlI CCTC 6 cut(s) 45, 296, 363, 391, 529, 572
MseI TTAA 1 cut(s) 132
MspI CCGG 2 cut(s) 343, 452
NdeI CATATG 1 cut(s) 314
NdeII GATC 4 cut(s) 29, 349, 471, 541
NlaIII CATG 3 cut(s) 53, 62, 570
NmuCI GTSAC 3 cut(s) 486, 505, 557
NspV TTCGAA 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PfeI GAWTC 3 cut(s) 187, 411, 459
PkrI GCNGC 2 cut(s) 257, 334
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PsiI TTATAA 1 cut(s) 153
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 2 cut(s) 256, 333
Sau3AI GATC 4 cut(s) 29, 349, 471, 541
SchI GAGTC 1 cut(s) 48
SetI ASST 8 cut(s) 105, 116, 127, 131, 260, 388, 447, 564
SfuI TTCGAA 1 cut(s) 525
Sse9I AATT 1 cut(s) 158
SsiI CCGC 1 cut(s) 333
StyI CCWWGG 2 cut(s) 114, 415
TaaI ACNGT 5 cut(s) 85, 281, 432, 505, 557
TaqI TCGA 6 cut(s) 28, 359, 500, 525, 534, 548
TasI AATT 1 cut(s) 158
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 3 cut(s) 187, 411, 459
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 284
TseFI GTSAC 3 cut(s) 486, 505, 557
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 3 cut(s) 486, 505, 557
TspDTI ATGAA 4 cut(s) 85, 310, 451, 455
TspGWI ACGGA 2 cut(s) 464, 504
TspRI CASTG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.