AT4G06643

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005302
Physical Location & Seq
Forward (+)
3758957 .. 3759880
924 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G06643.1

Sequence Viewer

Length: 786 bp
ATGGGGAGGAAGACAAAGAGAAAACTGCTTAACCTAATCCTGCTGAATGTTCCCGGATTGGGCCATGCCCAAGAACGCTTTTCACCCCCCTGCAGCCCATTAGACAATCTATCTCAGCCCAAAAGGAAAGTTTTTGGTAGAAGCCCAACTTTCATAAGGCCCAGGTCTGTTCTTTCTTTTTTCTTGGCAAAACAATTTTTGACGAGCTCTTACTCGATAAAAGAGGGTAGTGGACGCCTTCTTAGTTATGTAGTGAGAGAGTTAGGTTGGTCTTGTCTTTTCTTTTCACAAAACCTGGTGATTTCAAACCTCTACGCCTTCACGCTTAATCATCCCCTCTCAATCATAAGGGGCTATGCATCTCAACTCTCTCGACCATTATTGGAGAAACGGCCTTGGTATGTGCAACTTAACTCTTTCTCAAGCCTTTTTACTTCAATAATCGATGGTCATGTTTCGGTTGGCCATGGACACCAATCTACCTTCGCATCGTTCATTTGGTTATGGCCTTGTGATTATCTATGGCGTTTCCCACAAACCTTCTTCACCCTTTGGCCATTATTTCCATCGAAATCCTCTTTGGAACTTTTCAAAAGCTCTTATGCGTCAACCGCACGAGCCTTCATTGACCACCAGCTGGTCCGGGAATTCTCGAAGCTCGTCTTCAAGCATGATTCCCTTTTGGCTTCAACAGATTCTCCAAGTCTTGCAAGCTTCTCAAAGTCGAACACCACTTTGAAAAACTCATGGAGCCCTTATCTTTATGTTCCTTGTAATTTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.87

Weight (kDa)

10.05

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 637
AciI CCGC 1 cut(s) 612
AcoI YGGCCR 2 cut(s) 463, 554
AcsI RAATTY 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 59, 637
AgsI TTSAA 6 cut(s) 306, 438, 592, 667, 690, 739
AjnI CCWGG 2 cut(s) 161, 294
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AluBI AGCT 5 cut(s) 207, 597, 637, 658, 714
AluI AGCT 5 cut(s) 207, 597, 637, 658, 714
Alw21I GWGCWC 1 cut(s) 209
AoxI GGCC 6 cut(s) 61, 158, 392, 463, 506, 554
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 647
AspS9I GGNCC 3 cut(s) 61, 159, 640
AsuC2I CCSGG 2 cut(s) 54, 644
AsuHPI GGTGA 3 cut(s) 75, 310, 538
AvaII GGWCC 1 cut(s) 640
BalI TGGCCA 2 cut(s) 465, 556
BanII GRGCYC 2 cut(s) 209, 755
BauI CACGAG 1 cut(s) 615
BbsI GAAGAC 2 cut(s) 17, 655
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 105
BccI CCATC 2 cut(s) 440, 574
BceAI ACGGC 1 cut(s) 407
BciT130I CCWGG 2 cut(s) 163, 296
BcnI CCSGG 2 cut(s) 54, 644
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme1390I CCNGG 4 cut(s) 54, 163, 296, 644
Bme18I GGWCC 1 cut(s) 640
BmgT120I GGNCC 3 cut(s) 61, 159, 640
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 4 cut(s) 54, 163, 296, 644
BmsI GCATC 2 cut(s) 368, 497
BpiI GAAGAC 2 cut(s) 17, 655
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 2 cut(s) 54, 644
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 161, 395, 466
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 637
BseBI CCWGG 2 cut(s) 163, 296
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 161, 395, 466
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 59, 637
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 105
BshFI GGCC 6 cut(s) 63, 160, 394, 465, 508, 556
BshVI ATCGAT 1 cut(s) 444
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 2 cut(s) 54, 643
BslI CCNNNNNNNGG 2 cut(s) 59, 637
BsnI GGCC 6 cut(s) 63, 160, 394, 465, 508, 556
Bsp1286I GDGCHC 2 cut(s) 209, 755
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 1 cut(s) 612
BspANI GGCC 6 cut(s) 63, 160, 394, 465, 508, 556
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 752
BspMAI CTGCAG 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 161, 395, 466
BssNI GRCGYC 1 cut(s) 235
BssSI CACGAG 1 cut(s) 615
BssT1I CCWWGG 2 cut(s) 395, 466
Bst2BI CACGAG 1 cut(s) 615
Bst2UI CCWGG 2 cut(s) 163, 296
BstACI GRCGYC 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 712
BstDEI CTNAG 2 cut(s) 114, 242
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 611
BstNI CCWGG 2 cut(s) 163, 296
BstSCI CCNGG 4 cut(s) 52, 161, 294, 642
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 2 cut(s) 17, 655
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 6 cut(s) 63, 160, 394, 465, 508, 556
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 712
Cfr13I GGNCC 3 cut(s) 61, 159, 640
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 2 cut(s) 243, 594
CsiI ACCWGGT 1 cut(s) 294
CviAII CATG 5 cut(s) 65, 452, 467, 671, 747
DdeI CTNAG 2 cut(s) 114, 242
EaeI YGGCCR 2 cut(s) 463, 554
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 395, 466
Eco24I GRGCYC 2 cut(s) 209, 755
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 207
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 161, 294
EcoT14I CCWWGG 2 cut(s) 395, 466
EcoT22I ATGCAT 1 cut(s) 361
EcoT38I GRGCYC 2 cut(s) 209, 755
ErhI CCWWGG 2 cut(s) 395, 466
FaeI CATG 5 cut(s) 68, 455, 470, 674, 750
FalI AAGNNNNNCTT 4 cut(s) 133, 165, 647, 679
FatI CATG 5 cut(s) 64, 451, 466, 670, 746
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 1 cut(s) 318
FriOI GRGCYC 2 cut(s) 209, 755
Fsp4HI GCNGC 1 cut(s) 94
GluI GCNGC 1 cut(s) 94
HaeIII GGCC 6 cut(s) 63, 160, 394, 465, 508, 556
HapII CCGG 2 cut(s) 54, 643
HgaI GACGC 2 cut(s) 243, 594
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 5 cut(s) 68, 455, 470, 674, 750
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 2 cut(s) 674, 695
HpaII CCGG 2 cut(s) 54, 643
HphI GGTGA 3 cut(s) 75, 310, 538
Hpy166II GTNNAC 2 cut(s) 233, 609
Hpy188III TCNNGA 2 cut(s) 372, 652
Hpy8I GTNNAC 2 cut(s) 233, 609
HpyAV CCTTC 5 cut(s) 248, 328, 493, 550, 631
HpyCH4V TGCA 4 cut(s) 93, 359, 406, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 611
HpyF3I CTNAG 2 cut(s) 114, 242
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 5 cut(s) 68, 455, 470, 674, 750
LmnI GCTCC 1 cut(s) 750
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 2 cut(s) 368, 497
MabI ACCWGGT 1 cut(s) 294
MboII GAAGA 3 cut(s) 22, 535, 655
MhlI GDGCHC 2 cut(s) 209, 755
MlsI TGGCCA 2 cut(s) 465, 556
MluCI AATT 3 cut(s) 194, 647, 775
MluNI TGGCCA 2 cut(s) 465, 556
MnlI CCTC 4 cut(s) 217, 320, 347, 586
Mox20I TGGCCA 2 cut(s) 465, 556
Mph1103I ATGCAT 1 cut(s) 361
MscI TGGCCA 2 cut(s) 465, 556
MseI TTAA 3 cut(s) 30, 327, 411
Msp20I TGGCCA 2 cut(s) 465, 556
MspA1I CMGCKG 1 cut(s) 637
MspI CCGG 2 cut(s) 54, 643
MspR9I CCNGG 4 cut(s) 54, 163, 296, 644
MvaI CCWGG 2 cut(s) 163, 296
MwoI GCNNNNNNNGC 1 cut(s) 611
NciI CCSGG 2 cut(s) 54, 644
NcoI CCATGG 1 cut(s) 466
NlaIII CATG 5 cut(s) 68, 455, 470, 674, 750
NlaIV GGNNCC 1 cut(s) 752
NsiI ATGCAT 1 cut(s) 361
PfeI GAWTC 2 cut(s) 674, 695
PflMI CCANNNNNTGG 1 cut(s) 637
PfoI TCCNGGA 2 cut(s) 52, 642
PkrI GCNGC 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 161, 294
PspGI CCWGG 2 cut(s) 161, 294
PspN4I GGNNCC 1 cut(s) 752
PspPI GGNCC 3 cut(s) 61, 159, 640
PstI CTGCAG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 637
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 3 cut(s) 30, 327, 411
SatI GCNGC 1 cut(s) 94
Sau96I GGNCC 3 cut(s) 61, 159, 640
ScrFI CCNGG 4 cut(s) 54, 163, 296, 644
SduI GDGCHC 2 cut(s) 209, 755
SexAI ACCWGGT 1 cut(s) 294
SfaNI GCATC 2 cut(s) 368, 497
SfcI CTRYAG 1 cut(s) 91
SinI GGWCC 1 cut(s) 640
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 3 cut(s) 194, 647, 775
SsiI CCGC 1 cut(s) 612
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 4 cut(s) 52, 161, 294, 642
StyI CCWWGG 2 cut(s) 395, 466
TaqI TCGA 6 cut(s) 215, 373, 444, 569, 653, 725
TasI AATT 3 cut(s) 194, 647, 775
TfiI GAWTC 2 cut(s) 674, 695
Tru1I TTAA 3 cut(s) 30, 327, 411
Tru9I TTAA 3 cut(s) 30, 327, 411
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 3 cut(s) 142, 484, 613
Van91I CCANNNNNTGG 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 640
XapI RAATTY 1 cut(s) 647
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.