AT3G01345

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005302
Physical Location & Seq
Forward (+)
129138 .. 130749
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01345.1

Sequence Viewer

Length: 1401 bp
ATGTTTCGCTTGTCTTTTCTCCCTTTGTATCCAAAGATTACCTTGACCACCATACCTCTGAACACGTTCACTTCTTGTGCTAGTTCTCCCCTTATCTTCTTTGCGACCTCCGTGGTTTATCCCTCTCCTCGGCTCCCCTTGAAGTCAATACCGGCATCTCCCTATTCCCCTCTGGATCGGAGCTTTGCTTTGACGCCGGAGCTGTTGAAGAGCTTTACCTTGGAAGATCCAACTTCTGCAGACCGAGCCTCATCGCAAACAAACTTCGTTTGGTTAGATCTTGTCTCTGACGCCCTCAATTCGGCAGCTGTAAACCCTAAGTCCGTTTTGCCGTCGCCGTCGTCGTTGGTGACAAAGAGAAGATGCGCTTGGCGGCTAAACCTAATGCTGCCTCAGGGTTTTCGCATTGGGCCCAACCCAAGCCCCTTCTGTCCTCCCCCTAACCCATTACCTAAACTATTAAAGCCCATGAAGCCTTCTGCTGGGTTAAGCCCATATCCTCTAAGGCCCAGGTCTGTTTTCAAATCTGTTTTACAAAGCCCAAATTTTTTGATGTTGTTATCATTTAGGGAGAGTAATGGACGCTTTCCTGTGATGGTGATGATGGATTTAGCTTGGATTAGTAGCTCTCTCTCACAAAACCTAGTTGATAGTCTATCTCGTTTGGTCCGTTTTCTTCAGTGTTTGAGTCTAGTCTTGAATCGTTTGGAGCTATTTTCTCTTTGGAAGTGTCCTAGAGTGTTGCTGCCCACACCAAGTATCGTTAATTTACTATCGAGTTCCCTCCTTTTAGCACCCTCTACCTCTGTTTGGGCTGGTAATGGACGCCTTGCTATCAGAATAGTGGTTTGTTTAGGTGGGAATGGTAGTTACCATTGTCGAGATAAGATGTTAAGTTTTCTTTGGACCCTATCCGAGACTCCTTTACCCACCCATAGGCAAATGGCCTTTAGAGTTAAGAGGATCGGCATTATGACCCTGTCTCTACCGAGTAGGTGTTACCGAAGCCTTGAAAACTCTCAACTTATCCACTCACCATTTATCGCGCTCATTCATGTCATGTTAATCTATTGCTTGGATAATTTGCAATCTCTTGACATGTTGAAAAAGTACGGCATTATGACCATGTCTTCAAGAAGTGGTTACCGTCTTTTTCTCAACCTCGTTAACCCGTCAGCCTCCTCAACGGAGCACTTCTTCAAAAGCTCGGATGCATTGTTTGCAAGAGCTTCTTTTGACCACAAGATGGTCGAGGATTTCGCGAAGCCCGCCTTCATGGTCGAATCTGCCAAGGCCTCAACAGATTTTTCAATCATTAGAAAACTCATGAAGCTTCTATCCTTATTTTCCTGTAATTTCTTATTTTCATTGTGTATGAACTTGTCTCTTTTGAGATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

466

Amino Acids

52.2

Weight (kDa)

10.01

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1246
AccII CGCG 2 cut(s) 1046, 1262
AciI CCGC 2 cut(s) 373, 1269
AclWI GGATC 3 cut(s) 183, 221, 971
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 1 cut(s) 662
AcyI GRCGYC 3 cut(s) 194, 291, 826
AfaI GTAC 1 cut(s) 1112
AfiI CCNNNNNNNGG 6 cut(s) 129, 301, 482, 810, 936, 1246
AflIII ACRYGT 2 cut(s) 63, 1098
AgsI TTSAA 9 cut(s) 142, 208, 523, 700, 1013, 1105, 1134, 1201, 1311
AjnI CCWGG 1 cut(s) 509
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
Alw21I GWGCWC 1 cut(s) 1194
Alw26I GTCTC 4 cut(s) 289, 911, 987, 1389
AlwI GGATC 3 cut(s) 183, 221, 971
AoxI GGCC 4 cut(s) 410, 506, 945, 1293
ApaI GGGCCC 1 cut(s) 414
ApeKI GCWGC 3 cut(s) 305, 388, 745
ApoI RAATTY 1 cut(s) 544
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 2 cut(s) 368, 1048
AspS9I GGNCC 5 cut(s) 410, 411, 507, 667, 906
AsuHPI GGTGA 3 cut(s) 361, 610, 1026
AvaII GGWCC 2 cut(s) 667, 906
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 1 cut(s) 414
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 1122
Bbv12I GWGCWC 1 cut(s) 1194
BbvI GCAGC 3 cut(s) 317, 375, 732
BccI CCATC 3 cut(s) 589, 598, 1240
BceAI ACGGC 3 cut(s) 316, 322, 1129
BciT130I CCWGG 1 cut(s) 511
BciVI GTATCC 1 cut(s) 39
BcoDI GTCTC 4 cut(s) 289, 911, 987, 1389
BfaI CTAG 4 cut(s) 81, 644, 692, 735
BfmI CTRYAG 1 cut(s) 237
BfuI GTATCC 1 cut(s) 39
BglII AGATCT 1 cut(s) 277
BisI GCNGC 4 cut(s) 306, 374, 389, 746
BlsI GCNGC 4 cut(s) 307, 375, 390, 747
Bme1390I CCNGG 1 cut(s) 511
Bme18I GGWCC 2 cut(s) 667, 906
BmgT120I GGNCC 5 cut(s) 410, 411, 507, 667, 906
BmiI GGNNCC 3 cut(s) 134, 412, 908
BmrFI CCNGG 1 cut(s) 511
BmsI GCATC 3 cut(s) 164, 353, 1201
BpiI GAAGAC 1 cut(s) 1122
BsaHI GRCGYC 3 cut(s) 194, 291, 826
BsaJI CCNNGG 5 cut(s) 111, 128, 219, 509, 1290
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 301, 482, 810, 936, 1246
Bse118I RCCGGY 1 cut(s) 151
Bse21I CCTNAGG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 511
BseDI CCNNGG 5 cut(s) 111, 128, 219, 509, 1290
BseGI GGATG 1 cut(s) 1216
BseLI CCNNNNNNNGG 6 cut(s) 129, 301, 482, 810, 936, 1246
BseMII CTCAG 1 cut(s) 407
BseRI GAGGAG 2 cut(s) 117, 1171
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 3 cut(s) 317, 375, 732
BseYI CCCAGC 1 cut(s) 482
Bsh1236I CGCG 2 cut(s) 1046, 1262
BshFI GGCC 4 cut(s) 412, 508, 947, 1295
BsiHKAI GWGCWC 1 cut(s) 1194
BsiSI CCGG 2 cut(s) 152, 197
BslI CCNNNNNNNGG 6 cut(s) 129, 301, 482, 810, 936, 1246
BsmAI GTCTC 4 cut(s) 289, 911, 987, 1389
BsnI GGCC 4 cut(s) 412, 508, 947, 1295
Bsp120I GGGCCC 1 cut(s) 410
Bsp1286I GDGCHC 2 cut(s) 414, 1194
Bsp143I GATC 4 cut(s) 175, 226, 277, 963
Bsp68I TCGCGA 1 cut(s) 1262
BspACI CCGC 2 cut(s) 373, 1269
BspANI GGCC 4 cut(s) 412, 508, 947, 1295
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 2 cut(s) 1046, 1262
BspHI TCATGA 1 cut(s) 1326
BspLI GGNNCC 3 cut(s) 134, 412, 908
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 3 cut(s) 183, 221, 971
BspQI GCTCTTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 5 cut(s) 111, 128, 219, 509, 1290
BssMI GATC 4 cut(s) 175, 226, 277, 963
BssNI GRCGYC 3 cut(s) 194, 291, 826
BssT1I CCWWGG 2 cut(s) 219, 1290
Bst2UI CCWGG 1 cut(s) 511
Bst4CI ACNGT 1 cut(s) 1148
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 3 cut(s) 194, 291, 826
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstC8I GCNNGC 1 cut(s) 1269
BstDEI CTNAG 3 cut(s) 318, 393, 503
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 1142
BstF5I GGATG 1 cut(s) 1216
BstFNI CGCG 2 cut(s) 1046, 1262
BstHHI GCGC 2 cut(s) 368, 1048
BstKTI GATC 4 cut(s) 178, 229, 280, 966
BstMAI GTCTC 4 cut(s) 289, 911, 987, 1389
BstMBI GATC 4 cut(s) 175, 226, 277, 963
BstMWI GCNNNNNNNGC 4 cut(s) 245, 472, 1220, 1268
BstNI CCWGG 1 cut(s) 511
BstNSI RCATGY 1 cut(s) 1102
BstPI GGTNACC 1 cut(s) 1142
BstSCI CCNGG 1 cut(s) 509
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 414
BstUI CGCG 2 cut(s) 1046, 1262
BstV1I GCAGC 3 cut(s) 317, 375, 732
BstV2I GAAGAC 1 cut(s) 1122
BstX2I RGATCY 2 cut(s) 226, 277
BstYI RGATCY 2 cut(s) 226, 277
Bsu36I CCTNAGG 1 cut(s) 393
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 4 cut(s) 412, 508, 947, 1295
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 1216
BtsIMutI CAGTG 1 cut(s) 686
BtuMI TCGCGA 1 cut(s) 1262
Cac8I GCNNGC 1 cut(s) 1269
CciI TCATGA 1 cut(s) 1326
CfoI GCGC 2 cut(s) 368, 1048
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 5 cut(s) 410, 411, 507, 667, 906
CseI GACGC 4 cut(s) 202, 299, 591, 834
Csp6I GTAC 1 cut(s) 1111
CviAII CATG 7 cut(s) 469, 1055, 1060, 1099, 1126, 1276, 1327
CviQI GTAC 1 cut(s) 1111
DdeI CTNAG 3 cut(s) 318, 393, 503
DpnI GATC 4 cut(s) 177, 228, 279, 965
DpnII GATC 4 cut(s) 175, 226, 277, 963
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 219, 1290
Eco147I AGGCCT 1 cut(s) 1295
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 667, 906
Eco57I CTGAAG 1 cut(s) 662
Eco81I CCTNAGG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 1142
EcoO65I GGTNACC 1 cut(s) 1142
EcoRII CCWGG 1 cut(s) 509
EcoT14I CCWWGG 2 cut(s) 219, 1290
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 219, 1290
FaeI CATG 7 cut(s) 472, 1058, 1063, 1102, 1129, 1279, 1330
FalI AAGNNNNNCTT 8 cut(s) 26, 58, 352, 384, 1216, 1248, 1256, 1288
FatI CATG 7 cut(s) 468, 1054, 1059, 1098, 1125, 1275, 1326
FauI CCCGC 1 cut(s) 1276
Fnu4HI GCNGC 4 cut(s) 306, 374, 389, 746
FokI GGATG 1 cut(s) 1223
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 4 cut(s) 306, 374, 389, 746
FspBI CTAG 4 cut(s) 81, 644, 692, 735
GlaI GCGC 2 cut(s) 367, 1047
GluI GCNGC 4 cut(s) 306, 374, 389, 746
GsaI CCCAGC 1 cut(s) 486
HaeIII GGCC 4 cut(s) 412, 508, 947, 1295
HapII CCGG 2 cut(s) 152, 197
HgaI GACGC 4 cut(s) 202, 299, 591, 834
HhaI GCGC 2 cut(s) 368, 1048
Hin1I GRCGYC 3 cut(s) 194, 291, 826
Hin1II CATG 7 cut(s) 472, 1058, 1063, 1102, 1129, 1279, 1330
Hin6I GCGC 2 cut(s) 366, 1046
HinP1I GCGC 2 cut(s) 366, 1046
HincII GTYRAC 1 cut(s) 1168
HindII GTYRAC 1 cut(s) 1168
HindIII AAGCTT 1 cut(s) 1331
HinfI GANTC 4 cut(s) 688, 700, 919, 1283
HpaI GTTAAC 1 cut(s) 1168
HpaII CCGG 2 cut(s) 152, 197
HphI GGTGA 3 cut(s) 361, 610, 1026
Hpy166II GTNNAC 3 cut(s) 69, 313, 1168
Hpy188I TCNGA 6 cut(s) 60, 180, 289, 839, 916, 1210
Hpy188III TCNNGA 7 cut(s) 173, 697, 881, 1094, 1134, 1261, 1327
Hpy8I GTNNAC 3 cut(s) 69, 313, 1168
Hpy99I CGWCG 3 cut(s) 337, 343, 346
HpyAV CCTTC 3 cut(s) 436, 486, 1282
HpyCH4III ACNGT 1 cut(s) 1148
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 4 cut(s) 239, 1087, 1214, 1223
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 472, 1220, 1268
HpyF3I CTNAG 3 cut(s) 318, 393, 503
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 3 cut(s) 194, 291, 826
Hsp92II CATG 7 cut(s) 472, 1058, 1063, 1102, 1129, 1279, 1330
HspAI GCGC 2 cut(s) 366, 1046
KspAI GTTAAC 1 cut(s) 1168
Kzo9I GATC 4 cut(s) 175, 226, 277, 963
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 5 cut(s) 138, 180, 199, 709, 1189
Lsp1109I GCAGC 3 cut(s) 317, 375, 732
LweI GCATC 3 cut(s) 164, 353, 1201
MaeI CTAG 4 cut(s) 81, 644, 692, 735
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 4 cut(s) 349, 869, 998, 1142
MalI GATC 4 cut(s) 177, 228, 279, 965
MboI GATC 4 cut(s) 175, 226, 277, 963
MboII GAAGA 7 cut(s) 88, 220, 236, 372, 668, 1122, 1189
MflI RGATCY 2 cut(s) 226, 277
MhlI GDGCHC 2 cut(s) 414, 1194
MluCI AATT 5 cut(s) 298, 544, 766, 1081, 1354
MlyI GAGTC 2 cut(s) 697, 913
MmeI TCCRAC 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 1216
MroXI GAANNNNTTC 1 cut(s) 65
MseI TTAA 8 cut(s) 461, 488, 765, 893, 957, 1064, 1167, 1399
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 152, 197
MspR9I CCNGG 1 cut(s) 511
MvaI CCWGG 1 cut(s) 511
MvnI CGCG 2 cut(s) 1046, 1262
MwoI GCNNNNNNNGC 4 cut(s) 245, 472, 1220, 1268
NdeII GATC 4 cut(s) 175, 226, 277, 963
NlaIII CATG 7 cut(s) 472, 1058, 1063, 1102, 1129, 1279, 1330
NlaIV GGNNCC 3 cut(s) 134, 412, 908
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 349
NruI TCGCGA 1 cut(s) 1262
NsiI ATGCAT 1 cut(s) 1216
NspI RCATGY 1 cut(s) 1102
PagI TCATGA 1 cut(s) 1326
PceI AGGCCT 1 cut(s) 1295
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 1 cut(s) 203
PcsI WCGNNNNNNNCGW 2 cut(s) 341, 667
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 700, 1283
PflFI GACNNNGTC 2 cut(s) 979, 1126
PflMI CCANNNNNTGG 1 cut(s) 1246
PkrI GCNGC 4 cut(s) 307, 375, 390, 747
PleI GAGTC 2 cut(s) 696, 913
PpsI GAGTC 2 cut(s) 696, 913
PscI ACATGT 1 cut(s) 1098
Psp6I CCWGG 1 cut(s) 509
PspEI GGTNACC 1 cut(s) 1142
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 509
PspN4I GGNNCC 3 cut(s) 134, 412, 908
PspOMI GGGCCC 1 cut(s) 410
PspPI GGNCC 5 cut(s) 410, 411, 507, 667, 906
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 226, 277
PsyI GACNNNGTC 2 cut(s) 979, 1126
PvuII CAGCTG 1 cut(s) 308
RruI TCGCGA 1 cut(s) 1262
RsaI GTAC 1 cut(s) 1112
RsaNI GTAC 1 cut(s) 1111
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 8 cut(s) 461, 488, 765, 893, 957, 1064, 1167, 1399
SatI GCNGC 4 cut(s) 306, 374, 389, 746
Sau3AI GATC 4 cut(s) 175, 226, 277, 963
Sau96I GGNCC 5 cut(s) 410, 411, 507, 667, 906
SchI GAGTC 2 cut(s) 697, 913
ScrFI CCNGG 1 cut(s) 511
SduI GDGCHC 2 cut(s) 414, 1194
SfaNI GCATC 3 cut(s) 164, 353, 1201
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 2 cut(s) 667, 906
Sse9I AATT 5 cut(s) 298, 544, 766, 1081, 1354
SseBI AGGCCT 1 cut(s) 1295
SsiI CCGC 2 cut(s) 373, 1269
SspMI CTAG 4 cut(s) 81, 644, 692, 735
StuI AGGCCT 1 cut(s) 1295
StyD4I CCNGG 1 cut(s) 509
StyI CCWWGG 2 cut(s) 219, 1290
TaaI ACNGT 1 cut(s) 1148
TaiI ACGT 1 cut(s) 68
TaqI TCGA 4 cut(s) 776, 880, 1251, 1281
TaqII GACCGA 1 cut(s) 258
TasI AATT 5 cut(s) 298, 544, 766, 1081, 1354
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 2 cut(s) 700, 1283
Tru1I TTAA 8 cut(s) 461, 488, 765, 893, 957, 1064, 1167, 1399
Tru9I TTAA 8 cut(s) 461, 488, 765, 893, 957, 1064, 1167, 1399
TscAI CASTG 1 cut(s) 686
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 305, 388, 745
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 6 cut(s) 485, 1043, 1264, 1343, 1356, 1391
TspGWI ACGGA 4 cut(s) 100, 313, 659, 1202
TspRI CASTG 1 cut(s) 686
Tth111I GACNNNGTC 2 cut(s) 979, 1126
Van91I CCANNNNNTGG 1 cut(s) 1246
VpaK11BI GGWCC 2 cut(s) 667, 906
XapI RAATTY 1 cut(s) 544
XceI RCATGY 1 cut(s) 1102
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 4 cut(s) 81, 644, 692, 735
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.