AT4G08874

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005302
Physical Location & Seq
Reverse (-)
5673259 .. 5673809
551 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08874.2

Sequence Viewer

Length: 435 bp
ATGATCGATTACTCTCGGAAAACCTCCGGATTCAAATCTCGAACTGCACTCCTACTTGACCTAAAGGGTCCTCGGCGTCCAAGATCTTCCATCGTGGAGAGCACCTCGGCTCCGCCCTTCTCCTTTATGGAGAGAAACCGCTCAAATCTCTTAGTCGCTGCGGGGCTCGCACCGGCGCGTCATGAGGCTAAGAACCCCTTTCTAACGCCACAAAATCTATTGATAATGGCTATTTATGAAATTCCCGAATCTTTCGTGGAAGTGTCTTTGACACATCCTAGTCTCGCTTGTGATATGTTGCTAGTCAGCCTCTGTTTCCGAGATTCTAGGGACCTATCCTTTAAGCTTTTTGTTTGTCTTATCCATCTCTTGTATGTTTGCAAGATTATGCTTCAAAACTTTGTATCCTATGTACCCCTCTTTGGGTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.3

Weight (kDa)

9.02

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 141
AccII CGCG 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 26
AciI CCGC 3 cut(s) 113, 139, 161
AcsI RAATTY 1 cut(s) 240
AcyI GRCGYC 1 cut(s) 76
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 2 cut(s) 422, 423
AgsI TTSAA 2 cut(s) 34, 395
AluBI AGCT 1 cut(s) 346
AluI AGCT 1 cut(s) 346
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 287
AlwNI CAGNNNCTG 1 cut(s) 312
Aor13HI TCCGGA 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 240
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 68, 331
AvaII GGWCC 2 cut(s) 68, 331
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 145
BccI CCATC 2 cut(s) 98, 372
BciVI GTATCC 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 3 cut(s) 279, 302, 327
BfuI GTATCC 1 cut(s) 415
BglII AGATCT 1 cut(s) 83
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 2 cut(s) 68, 331
BmgT120I GGNCC 2 cut(s) 68, 331
BmiI GGNNCC 3 cut(s) 69, 111, 332
BplI GAGNNNNNCTC 2 cut(s) 89, 121
Bsa29I ATCGAT 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 71, 105
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 423
Bse118I RCCGGY 1 cut(s) 172
Bse8I GATNNNNATC 1 cut(s) 34
BseAI TCCGGA 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 2 cut(s) 71, 105
BseGI GGATG 1 cut(s) 274
BseJI GATNNNNATC 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 422, 423
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 178
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 2 cut(s) 27, 173
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 422, 423
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 104, 168
Bsp13I TCCGGA 1 cut(s) 26
Bsp143I GATC 2 cut(s) 3, 83
BspACI CCGC 3 cut(s) 113, 139, 161
BspDI ATCGAT 1 cut(s) 6
BspEI TCCGGA 1 cut(s) 26
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 69, 111, 332
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 71, 105
BssMI GATC 2 cut(s) 3, 83
BssNI GRCGYC 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 2 cut(s) 151, 189
BstF5I GGATG 1 cut(s) 274
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 2 cut(s) 6, 86
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 3, 83
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
Bsu15I ATCGAT 1 cut(s) 6
BsuI GTATCC 1 cut(s) 415
BsuTUI ATCGAT 1 cut(s) 6
BtsCI GGATG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 312
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 68, 331
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 2 cut(s) 65, 167
Csp6I GTAC 1 cut(s) 413
CviAII CATG 1 cut(s) 182
CviJI RGCY 6 cut(s) 110, 166, 188, 230, 309, 346
CviKI_1 RGCY 6 cut(s) 110, 166, 188, 230, 309, 346
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 2 cut(s) 151, 189
DpnI GATC 2 cut(s) 5, 85
DpnII GATC 2 cut(s) 3, 83
EciI GGCGGA 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 68, 331
EcoO109I RGGNCCY 2 cut(s) 68, 331
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 1 cut(s) 185
FaiI YATR 7 cut(s) 128, 183, 237, 296, 375, 389, 411
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 181
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 261
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 3 cut(s) 279, 302, 327
GlaI GCGC 1 cut(s) 177
GluI GCNGC 1 cut(s) 159
HapII CCGG 2 cut(s) 27, 173
HgaI GACGC 2 cut(s) 65, 167
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 76
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 3 cut(s) 30, 248, 323
HpaII CCGG 2 cut(s) 27, 173
Hpy188I TCNGA 2 cut(s) 18, 320
Hpy188III TCNNGA 4 cut(s) 27, 39, 182, 245
HpyAV CCTTC 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 47, 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 2 cut(s) 151, 189
Hsp92I GRCGYC 1 cut(s) 76
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 1 cut(s) 176
Kpn2I TCCGGA 1 cut(s) 26
Kzo9I GATC 2 cut(s) 3, 83
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 2 cut(s) 40, 186
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 3 cut(s) 279, 302, 327
MalI GATC 2 cut(s) 5, 85
MbiI CCGCTC 1 cut(s) 141
MboI GATC 2 cut(s) 3, 83
MboII GAAGA 1 cut(s) 78
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 2 cut(s) 104, 168
MluCI AATT 1 cut(s) 240
MnlI CCTC 6 cut(s) 34, 81, 115, 178, 320, 428
MroI TCCGGA 1 cut(s) 26
MseI TTAA 1 cut(s) 342
MspI CCGG 2 cut(s) 27, 173
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 2 cut(s) 3, 83
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 3 cut(s) 69, 111, 332
NmeAIII GCCGAG 2 cut(s) 52, 86
PagI TCATGA 1 cut(s) 181
PfeI GAWTC 3 cut(s) 30, 248, 323
PkrI GCNGC 1 cut(s) 160
PpuMI RGGWCCY 2 cut(s) 68, 331
Psp5II RGGWCCY 2 cut(s) 68, 331
PspN4I GGNNCC 3 cut(s) 69, 111, 332
PspPI GGNCC 2 cut(s) 68, 331
PspPPI RGGWCCY 2 cut(s) 68, 331
PstNI CAGNNNCTG 1 cut(s) 312
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 3, 83
Sau96I GGNCC 2 cut(s) 68, 331
SduI GDGCHC 2 cut(s) 104, 168
SetI ASST 5 cut(s) 26, 63, 107, 336, 348
SgrAI CRCCGGYG 1 cut(s) 172
SinI GGWCC 2 cut(s) 68, 331
Sse9I AATT 1 cut(s) 240
SsiI CCGC 3 cut(s) 113, 139, 161
SspMI CTAG 3 cut(s) 279, 302, 327
TaqI TCGA 2 cut(s) 6, 40
TasI AATT 1 cut(s) 240
TfiI GAWTC 3 cut(s) 30, 248, 323
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 252
VpaK11BI GGWCC 2 cut(s) 68, 331
XapI RAATTY 1 cut(s) 240
XspI CTAG 3 cut(s) 279, 302, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.