AT5G28919

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005302
Physical Location & Seq
Forward (+)
10940033 .. 10941771
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28919.1

Sequence Viewer

Length: 567 bp
ATGGATGAGAGCTCGGCCTCGACGTGGAAACTGTGGAGGCTTCAAATCACAAAAGAATCTTCTTCTGCAGACCGAGATTCATCACTCGCACCTCTCGATGTGGTCGTTTTAGTCTTAGAAGCCCTCAATTCGGTCACCAGAATGTTGCATTCAGTTGTGCCACCGCCGCTTTTGGTGAGGAAGAGAAGAACCTGGCTAAACCTAATGGGTCTTCGGTTTGGGCCAAGCCCAAGCCTCCTTTGCCCTCCCTCCTGTCCTTTACCCAAAGCTTTGAAGCCCACAAAGACTTCTGCTGACTTATGCCCACGTCTTCTAAGGCCCTGGTCTTGTTTTAATTCTATTCTGAACAGCCCAAATTTTCTGAAACTGTCATCGTTTAGGGATTGTTATGGACGCCTTCCTGTAATGGTGATGATGGATTTAGTTTGGATTAGTTGCTATCTTTCACAAAACCTGGTTGCTAGTTCTTATCGTGTGGTCCGTTTGCGTGATTGTCCGAGTCTGATCTCAAGTAGTTTGGAAGTAGTTTCTCTTTGGAAGTGTCCTAGATTGTTGTTGCCCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.16

Weight (kDa)

9.65

Isoelectric Point (pI)

57.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 164, 167
AcsI RAATTY 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 24, 130
AgsI TTSAA 2 cut(s) 44, 274
AjiI CACGTC 2 cut(s) 24, 308
AjnI CCWGG 3 cut(s) 191, 320, 453
AluBI AGCT 2 cut(s) 12, 269
AluI AGCT 2 cut(s) 12, 269
Alw21I GWGCWC 1 cut(s) 14
AoxI GGCC 3 cut(s) 15, 221, 317
ApoI RAATTY 1 cut(s) 355
AspS9I GGNCC 3 cut(s) 221, 318, 478
AsuHPI GGTGA 3 cut(s) 127, 187, 421
AvaII GGWCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 203, 302
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 1 cut(s) 409
BciT130I CCWGG 3 cut(s) 193, 322, 455
BfaI CTAG 2 cut(s) 462, 546
BfmI CTRYAG 1 cut(s) 66
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 3 cut(s) 193, 322, 455
Bme18I GGWCC 1 cut(s) 478
BmgBI CACGTC 2 cut(s) 24, 308
BmgT120I GGNCC 3 cut(s) 221, 318, 478
BmrFI CCNGG 3 cut(s) 193, 322, 455
BpiI GAAGAC 2 cut(s) 203, 302
BpuEI CTTGAG 1 cut(s) 493
BsaHI GRCGYC 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 130
BseBI CCWGG 3 cut(s) 193, 322, 455
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 24, 130
BshFI GGCC 3 cut(s) 17, 223, 319
BsiHKAI GWGCWC 1 cut(s) 14
BslI CCNNNNNNNGG 2 cut(s) 24, 130
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 3 cut(s) 17, 223, 319
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 1 cut(s) 504
BspACI CCGC 2 cut(s) 164, 167
BspANI GGCC 3 cut(s) 17, 223, 319
BspMAI CTGCAG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 1 cut(s) 504
BssNI GRCGYC 1 cut(s) 394
Bst2UI CCWGG 3 cut(s) 193, 322, 455
Bst4CI ACNGT 2 cut(s) 33, 369
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 115, 314
BstEII GGTNACC 1 cut(s) 133
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 1 cut(s) 507
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 2 cut(s) 166, 240
BstNI CCWGG 3 cut(s) 193, 322, 455
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 3 cut(s) 191, 320, 453
BstSFI CTRYAG 1 cut(s) 66
BstV2I GAAGAC 2 cut(s) 203, 302
BsuRI GGCC 3 cut(s) 17, 223, 319
BtrI CACGTC 2 cut(s) 24, 308
BtsCI GGATG 1 cut(s) 10
Cfr13I GGNCC 3 cut(s) 221, 318, 478
CseI GACGC 1 cut(s) 402
CsiI ACCWGGT 1 cut(s) 453
DdeI CTNAG 2 cut(s) 115, 314
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 133
EcoRII CCWGG 3 cut(s) 191, 320, 453
EcoT38I GRGCYC 1 cut(s) 14
FaiI YATR 2 cut(s) 301, 390
Fnu4HI GCNGC 1 cut(s) 167
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 1 cut(s) 167
FspBI CTAG 2 cut(s) 462, 546
GluI GCNGC 1 cut(s) 167
HaeIII GGCC 3 cut(s) 17, 223, 319
HgaI GACGC 1 cut(s) 402
Hin1I GRCGYC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 3 cut(s) 56, 77, 499
HphI GGTGA 3 cut(s) 127, 187, 421
Hpy188I TCNGA 4 cut(s) 345, 363, 498, 504
Hpy188III TCNNGA 1 cut(s) 95
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 2 cut(s) 33, 369
HpyCH4IV ACGT 2 cut(s) 23, 307
HpyCH4V TGCA 2 cut(s) 68, 148
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 240
HpyF3I CTNAG 2 cut(s) 115, 314
HpySE526I ACGT 2 cut(s) 23, 307
Hsp92I GRCGYC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 504
LpnPI CCDG 9 cut(s) 151, 178, 205, 265, 307, 334, 414, 440, 467
MabI ACCWGGT 1 cut(s) 453
MaeI CTAG 2 cut(s) 462, 546
MaeII ACGT 2 cut(s) 23, 307
MaeIII GTNAC 1 cut(s) 133
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MboII GAAGA 6 cut(s) 51, 54, 193, 198, 203, 302
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 3 cut(s) 127, 334, 355
MlyI GAGTC 1 cut(s) 508
MnlI CCTC 8 cut(s) 28, 30, 102, 134, 171, 245, 255, 259
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 140
MspR9I CCNGG 3 cut(s) 193, 322, 455
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 3 cut(s) 193, 322, 455
MwoI GCNNNNNNNGC 2 cut(s) 166, 240
NdeII GATC 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 133
PcsI WCGNNNNNNNCGW 4 cut(s) 20, 93, 102, 478
PctI GAATGC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 56, 77
PkrI GCNGC 1 cut(s) 168
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 3 cut(s) 191, 320, 453
PspEI GGTNACC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 191, 320, 453
PspPI GGNCC 3 cut(s) 221, 318, 478
PstI CTGCAG 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 140
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 3 cut(s) 221, 318, 478
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 3 cut(s) 193, 322, 455
SduI GDGCHC 1 cut(s) 14
SetI ASST 8 cut(s) 14, 26, 94, 194, 204, 271, 310, 456
SexAI ACCWGGT 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 66
SinI GGWCC 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 3 cut(s) 127, 334, 355
SsiI CCGC 2 cut(s) 164, 167
SspMI CTAG 2 cut(s) 462, 546
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 191, 320, 453
TaaI ACNGT 2 cut(s) 33, 369
TaiI ACGT 2 cut(s) 26, 310
TaqI TCGA 2 cut(s) 20, 96
TaqII GACCGA 2 cut(s) 87, 121
TasI AATT 3 cut(s) 127, 334, 355
TauI GCSGC 1 cut(s) 169
TfiI GAWTC 2 cut(s) 56, 77
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 69
TspGWI ACGGA 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 478
XapI RAATTY 1 cut(s) 355
XspI CTAG 2 cut(s) 462, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.