AT3G24542

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005302
Physical Location & Seq
Forward (+)
8956257 .. 8957667
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24542.1

Sequence Viewer

Length: 462 bp
ATGGTACCGTCACTCGTCGATCTTGAGTCTCTGGACCACTTTTGTGCCACAACTTCCGGTGAAGTTTCATTCCACTCTGATCTTCAACTCACCGAAGTCCTGCCGCCACAGTCACCAACTCTTCATCGCAGCCACAGAGACACTAGGTGCGGCATTATGATCCCGTCACCAAGGTGCGGTGATTACCGCAGCTTTCTCACCTTCCAATCTCCGTCTCCTTTGTTCACTGAGTTCTCTCTTATGAAATCTATCAATTTCAGTGATAAATTCCTTATGCTCATGAAAACCTTGAAGCTTAGCGATTTTTTTGAGTTTGACTACAGACCCTTACTCCTTCTCAACGTCATTATGTCTTGTGACAATGACCAATTTTCCACTTCTCGGATCGACCGAAGTTGTGTCTCCGATTTACCTTCTTCTCGTATGTGGCATTGTGTTCTACAACTTTTGTCTCCGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.51

Weight (kDa)

5.64

Isoelectric Point (pI)

64.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 4 cut(s) 104, 150, 177, 187
AclWI GGATC 2 cut(s) 154, 392
AcsI RAATTY 1 cut(s) 266
AdeI CACNNNGTG 1 cut(s) 147
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 2 cut(s) 176, 381
AgsI TTSAA 2 cut(s) 86, 292
AleI CACNNNNGTG 2 cut(s) 42, 172
AluBI AGCT 2 cut(s) 192, 295
AluI AGCT 2 cut(s) 192, 295
Alw26I GTCTC 5 cut(s) 33, 132, 219, 406, 456
AlwI GGATC 2 cut(s) 154, 392
ApeKI GCWGC 2 cut(s) 129, 189
ApoI RAATTY 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 4
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 6 cut(s) 71, 82, 105, 159, 190, 191
AvaII GGWCC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 141, 201
BcoDI GTCTC 5 cut(s) 33, 132, 219, 406, 456
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 319
BisI GCNGC 4 cut(s) 104, 130, 151, 190
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 4 cut(s) 105, 131, 152, 191
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 6
Bpu1102I GCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 170
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 381
BseDI CCNNGG 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 176, 381
BseMII CTCAG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 141, 201
Bsh1285I CGRYCG 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 391
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 176, 381
BsmAI GTCTC 5 cut(s) 33, 132, 219, 406, 456
BsmBI CGTCTC 1 cut(s) 219
Bsp143I GATC 4 cut(s) 19, 79, 159, 384
Bsp1720I GCTNAGC 1 cut(s) 296
BspACI CCGC 4 cut(s) 104, 150, 177, 187
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 154, 392
BspT107I GGYRCC 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 4 cut(s) 19, 79, 159, 384
BssT1I CCWWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 9, 111
Bst6I CTCTTC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 228, 296
BstKTI GATC 4 cut(s) 22, 82, 162, 387
BstMAI GTCTC 5 cut(s) 33, 132, 219, 406, 456
BstMBI GATC 4 cut(s) 19, 79, 159, 384
BstMCI CGRYCG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 141, 201
BtgZI GCGATG 1 cut(s) 110
BtsIMutI CAGTG 2 cut(s) 225, 265
CciI TCATGA 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 1 cut(s) 5
CviAII CATG 1 cut(s) 280
CviJI RGCY 3 cut(s) 132, 192, 295
CviKI_1 RGCY 3 cut(s) 132, 192, 295
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 2 cut(s) 228, 296
DpnI GATC 4 cut(s) 21, 81, 161, 386
DpnII GATC 4 cut(s) 19, 79, 159, 384
DraIII CACNNNGTG 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 1 cut(s) 283
FaiI YATR 6 cut(s) 158, 242, 275, 281, 350, 425
FatI CATG 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 104, 130, 151, 190
Fsp4HI GCNGC 4 cut(s) 104, 130, 151, 190
FspBI CTAG 1 cut(s) 144
GluI GCNGC 4 cut(s) 104, 130, 151, 190
HapII CCGG 1 cut(s) 57
Hin1II CATG 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 26
HpaII CCGG 1 cut(s) 57
HphI GGTGA 6 cut(s) 71, 82, 105, 159, 190, 191
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 4 cut(s) 79, 384, 406, 456
Hpy188III TCNNGA 3 cut(s) 23, 32, 280
Hpy8I GTNNAC 1 cut(s) 225
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 3 cut(s) 211, 344, 423
HpyCH4III ACNGT 2 cut(s) 9, 111
HpyCH4IV ACGT 1 cut(s) 342
HpyF3I CTNAG 2 cut(s) 228, 296
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 1 cut(s) 283
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 4 cut(s) 19, 79, 159, 384
LpnPI CCDG 3 cut(s) 17, 70, 113
Lsp1109I GCAGC 2 cut(s) 141, 201
MaeI CTAG 1 cut(s) 144
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 4 cut(s) 9, 111, 165, 356
MalI GATC 4 cut(s) 21, 81, 161, 386
MboI GATC 4 cut(s) 19, 79, 159, 384
MboII GAAGA 3 cut(s) 74, 113, 408
MluCI AATT 3 cut(s) 253, 266, 368
MlyI GAGTC 1 cut(s) 35
MslI CAYNNNNRTG 2 cut(s) 42, 172
MspI CCGG 1 cut(s) 57
NdeII GATC 4 cut(s) 19, 79, 159, 384
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 4 cut(s) 9, 111, 165, 356
OliI CACNNNNGTG 2 cut(s) 42, 172
PagI TCATGA 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PkrI GCNGC 4 cut(s) 105, 131, 152, 191
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 34
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
RseI CAYNNNNRTG 2 cut(s) 42, 172
SatI GCNGC 4 cut(s) 104, 130, 151, 190
Sau3AI GATC 4 cut(s) 19, 79, 159, 384
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 1 cut(s) 35
SetI ASST 8 cut(s) 149, 176, 194, 203, 290, 297, 345, 415
SfcI CTRYAG 1 cut(s) 319
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 42, 172
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 3 cut(s) 253, 266, 368
SsiI CCGC 4 cut(s) 104, 150, 177, 187
SspMI CTAG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 9, 111
TaiI ACGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 18, 387
TaqII GACCGA 1 cut(s) 405
TasI AATT 3 cut(s) 253, 266, 368
TauI GCSGC 2 cut(s) 106, 153
TscAI CASTG 2 cut(s) 232, 265
TseFI GTSAC 4 cut(s) 9, 111, 165, 356
TseI GCWGC 2 cut(s) 129, 189
Tsp45I GTSAC 4 cut(s) 9, 111, 165, 356
TspDTI ATGAA 4 cut(s) 57, 113, 257, 296
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 2 cut(s) 232, 265
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 1 cut(s) 266
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.