AT5G35760

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005302
Physical Location & Seq
Forward (+)
13934108 .. 13935088
981 bp
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UTR
Exon/CDS
Intron
AT5G35760.1

Sequence Viewer

Length: 534 bp
ATGTCCAAAAGAGTGAAGAAGAACGCCATTATGATCCCATCTCCACGGAGCGGTGGTTACCGAAGCTTCTTCAACTCTCTACCTCCTTACCCGCCAATTACCAAGCTCCTTCAAGGTATCTTCAAGTATACGGATAAGTTGCAAATCTGTGAAAGCTTGGCAAAGAGCGGCGTTATGATCTACCCTTTATTTTCTCCCGTGGAGCACCTTTTCAAAGGCTCATATGCATTGTTTGCAATAGCCGTTTATGACCACACGTTGGTCGAGGATTTCGCGAAGCCCGTCTTCATGGTCGAATCCGCCAAAGCCTCAACAGATTTTTCAAACCTTGTAAACTTTTTTAAAAGTGAACACCTCTTTGCAAAACTCATGAAGCCTCTATCTTCATTTTCTTGCAAAGCAATCCGACTAAAATCAAGGAGATGTGGAGGGGAGAAGCTTCCGCCTGATACCAACGGTAAGAGCTCCACATGCGGTGTAGCGATGCAGAGTAGAAAAGGGAGATCTACGGCGAAGAACGAGAGAGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

177

Amino Acids

19.77

Weight (kDa)

9.89

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AccBSI CCGCTC 2 cut(s) 51, 168
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 275
AciI CCGC 6 cut(s) 51, 92, 168, 300, 443, 474
AclWI GGATC 1 cut(s) 28
AfiI CCNNNNNNNGG 2 cut(s) 50, 259
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 5 cut(s) 73, 113, 124, 214, 324
AluBI AGCT 5 cut(s) 66, 106, 156, 439, 465
AluI AGCT 5 cut(s) 66, 106, 156, 439, 465
Alw21I GWGCWC 2 cut(s) 207, 467
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 207, 467
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 227, 525
BcoDI GTCTC 1 cut(s) 519
BglII AGATCT 1 cut(s) 503
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
BmsI GCATC 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 277
BsaJI CCNNGG 2 cut(s) 44, 198
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 259
BseDI CCNNGG 2 cut(s) 44, 198
BseLI CCNNNNNNNGG 2 cut(s) 50, 259
Bsh1236I CGCG 1 cut(s) 275
BsiHKAI GWGCWC 2 cut(s) 207, 467
BslI CCNNNNNNNGG 2 cut(s) 50, 259
BsmAI GTCTC 1 cut(s) 519
Bsp1286I GDGCHC 2 cut(s) 207, 467
Bsp143I GATC 3 cut(s) 33, 177, 503
Bsp68I TCGCGA 1 cut(s) 275
BspACI CCGC 6 cut(s) 51, 92, 168, 300, 443, 474
BspFNI CGCG 1 cut(s) 275
BspHI TCATGA 1 cut(s) 369
BspPI GGATC 1 cut(s) 28
BsrBI CCGCTC 2 cut(s) 51, 168
BssECI CCNNGG 2 cut(s) 44, 198
BssMI GATC 3 cut(s) 33, 177, 503
BssNAI GTATAC 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 233
BstDSI CCRYGG 2 cut(s) 44, 198
BstEII GGTNACC 1 cut(s) 56
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 3 cut(s) 36, 180, 506
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 3 cut(s) 33, 177, 503
BstMWI GCNNNNNNNGC 2 cut(s) 233, 471
BstNSI RCATGY 1 cut(s) 474
BstPI GGTNACC 1 cut(s) 56
BstUI CGCG 1 cut(s) 275
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BstZ17I GTATAC 1 cut(s) 129
BtgI CCRYGG 2 cut(s) 44, 198
BtgZI GCGATG 1 cut(s) 497
BtuMI TCGCGA 1 cut(s) 275
CciI TCATGA 1 cut(s) 369
CviAII CATG 3 cut(s) 289, 370, 471
DpnI GATC 3 cut(s) 35, 179, 505
DpnII GATC 3 cut(s) 33, 177, 503
DraI TTTAAA 1 cut(s) 343
EciI GGCGGA 2 cut(s) 289, 432
Ecl136II GAGCTC 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 465
Eco91I GGTNACC 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 465
EcoO65I GGTNACC 1 cut(s) 56
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 467
FaeI CATG 3 cut(s) 292, 373, 474
FaiI YATR 9 cut(s) 32, 129, 176, 223, 225, 249, 290, 371, 472
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 3 cut(s) 288, 369, 470
FauI CCCGC 1 cut(s) 99
FauNDI CATATG 1 cut(s) 223
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 169
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 292, 373, 474
HindIII AAGCTT 3 cut(s) 64, 154, 437
HinfI GANTC 1 cut(s) 296
Hpy166II GTNNAC 3 cut(s) 129, 334, 350
Hpy188I TCNGA 1 cut(s) 407
Hpy188III TCNNGA 2 cut(s) 274, 370
Hpy8I GTNNAC 3 cut(s) 129, 334, 350
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 1 cut(s) 458
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 6 cut(s) 142, 227, 236, 362, 396, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 471
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 3 cut(s) 292, 373, 474
Kzo9I GATC 3 cut(s) 33, 177, 503
LmnI GCTCC 4 cut(s) 48, 111, 202, 470
LpnPI CCDG 1 cut(s) 459
LweI GCATC 1 cut(s) 474
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 56
MalI GATC 3 cut(s) 35, 179, 505
MbiI CCGCTC 2 cut(s) 51, 168
MboI GATC 3 cut(s) 33, 177, 503
MboII GAAGA 7 cut(s) 28, 31, 61, 112, 277, 375, 526
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 2 cut(s) 207, 467
MluCI AATT 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 6 cut(s) 93, 259, 319, 365, 387, 422
Mph1103I ATGCAT 1 cut(s) 229
MseI TTAA 1 cut(s) 342
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 233, 471
NdeI CATATG 1 cut(s) 223
NdeII GATC 3 cut(s) 33, 177, 503
NlaIII CATG 3 cut(s) 292, 373, 474
NruI TCGCGA 1 cut(s) 275
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 1 cut(s) 474
PagI TCATGA 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 1 cut(s) 170
Psp124BI GAGCTC 1 cut(s) 467
PspEI GGTNACC 1 cut(s) 56
PsuI RGATCY 1 cut(s) 503
RruI TCGCGA 1 cut(s) 275
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 3 cut(s) 33, 177, 503
SduI GDGCHC 2 cut(s) 207, 467
SfaNI GCATC 1 cut(s) 474
Sse9I AATT 1 cut(s) 96
SsiI CCGC 6 cut(s) 51, 92, 168, 300, 443, 474
SstI GAGCTC 1 cut(s) 467
TaaI ACNGT 1 cut(s) 458
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 264, 294
TasI AATT 1 cut(s) 96
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TspDTI ATGAA 3 cut(s) 277, 375, 386
TspGWI ACGGA 2 cut(s) 61, 146
Van91I CCANNNNNTGG 1 cut(s) 259
XceI RCATGY 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.