AT2G21610

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003654
Physical Location & Seq
Reverse (-)
9245038 .. 9247052
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21610.1

Sequence Viewer

Length: 1059 bp
ATGGGACTTTATAAAACGAAGTCCAAACGTTCAATTGCTAACTATCATCATATCATAATCATCAATATTTTCATCCTCTCTTCAATCACATCTTCTTCCATGGCTTCTTCTTCTTCTCCATCTTCCATTGATTTTTCGACTGCAATTCTAATAAGAGTTGATCAATCGGGCAAAGGAGACTTCAGTAAGATTCAAGAAGCAATTGAATCTATCCCTCCCAATCTAAACAACTCTCAACTCTATTTCATTTGGGTTAAGCCTGGAATTTACCGAGAAAAAGTGGTGATACCTGCGGAGAAACCCTACATAACATTGAGTGGGACACAAGCTTCCAACACTTTTCTCATATGGAGCGATGGCGAAGACATTTTGGAATCACCCACTCTCACTATCTTTGCATCCGATTTTGTATGCCGATTTCTCACTATTCAGAATAAGTTTGGAACGGCGGGACGTGCGGTTGCACTGAGGGTGGCGGCGGATAAGGCAGCGTTCTACGGATGCGTGATAACGTCGTACCAAGACACTCTCCTTGACGACAATGGAAACCATTACTTCAAGAACTGCTATATTGAAGGAGCCACCGATTTCATTTGTGGAAGCGCTTCTTCTCTATATGAAAGGTGTCATTTACACTCGTTGTCGCCGAATAATGGGTCTATAACGGCGCAAATGAGAACGTCGGCCACAGAGAAATCAGGCTTTACATTCTTGGGATGCAAGTTAACTGGATCAGGTTCCACATTTTTGGGAAGACCATGGGGAGCTTATTCTAGGGTTGTCTTTGCTTATTCCTTCTTCTCCAATGTCGTCGCACCTCAAGGATGGAACCAATGGGGAGATTCAACCAAAGAAAATACTGTATATTATGGTGAATACAAATGTTATGGTCCCGGAGCAGACAGGGAACAAAGAGTAGAATGGTCAAAACAGTTATCGGATGAAGAAGCTACCGTCTTCTTGAGCAAGGACTTTATCGGCGGCAAAGACTGGCTCCGACCTGCCCCTTCTCACTTCAAGAATGCTCCCAAACAAACCCAAAATAAAGAAATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

352

Amino Acids

39.06

Weight (kDa)

8.06

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 53 - 328 1.1e-66 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
Acc36I ACCTGC 2 cut(s) 298, 1009
AciI CCGC 6 cut(s) 293, 449, 458, 476, 479, 981
AclI AACGTT 1 cut(s) 28
AclWI GGATC 1 cut(s) 739
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 166
AfaI GTAC 1 cut(s) 518
AfeI AGCGCT 1 cut(s) 604
AfiI CCNNNNNNNGG 1 cut(s) 653
AgsI TTSAA 8 cut(s) 33, 84, 194, 206, 559, 575, 846, 1018
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 3 cut(s) 329, 767, 950
AluI AGCT 3 cut(s) 329, 767, 950
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 739
Aor51HI AGCGCT 1 cut(s) 604
AoxI GGCC 1 cut(s) 684
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 264
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AseI ATTAAT 1 cut(s) 1053
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 605, 670
AspS9I GGNCC 1 cut(s) 890
AsuC2I CCSGG 1 cut(s) 894
AsuHPI GGTGA 3 cut(s) 295, 369, 884
AvaII GGWCC 1 cut(s) 890
BbsI GAAGAC 3 cut(s) 369, 760, 949
BbvI GCAGC 1 cut(s) 500
BccI CCATC 3 cut(s) 127, 350, 819
BceAI ACGGC 2 cut(s) 462, 681
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 1 cut(s) 894
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 774
BfoI RGCGCY 1 cut(s) 606
BfuAI ACCTGC 2 cut(s) 298, 1009
BisI GCNGC 3 cut(s) 477, 489, 982
BlsI GCNGC 3 cut(s) 478, 490, 983
Bme1390I CCNGG 2 cut(s) 261, 894
Bme18I GGWCC 1 cut(s) 890
BmgBI CACGTC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 890
BmiI GGNNCC 5 cut(s) 580, 739, 830, 892, 995
BmrFI CCNGG 2 cut(s) 261, 894
BmsI GCATC 3 cut(s) 407, 491, 707
BpiI GAAGAC 3 cut(s) 369, 760, 949
BpuEI CTTGAG 2 cut(s) 804, 982
BpuMI CCSGG 1 cut(s) 894
BsaJI CCNNGG 2 cut(s) 99, 758
Bsc4I CCNNNNNNNGG 1 cut(s) 653
Bse1I ACTGG 2 cut(s) 733, 995
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 99, 758
BseGI GGATG 6 cut(s) 72, 398, 506, 722, 830, 946
BseLI CCNNNNNNNGG 1 cut(s) 653
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 2 cut(s) 733, 995
BseXI GCAGC 1 cut(s) 500
BshFI GGCC 1 cut(s) 686
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 4 cut(s) 18, 334, 465, 876
BslI CCNNNNNNNGG 1 cut(s) 653
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 4 cut(s) 18, 334, 465, 876
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 1 cut(s) 686
Bsp143I GATC 2 cut(s) 160, 731
Bsp19I CCATGG 2 cut(s) 99, 758
BspACI CCGC 6 cut(s) 293, 449, 458, 476, 479, 981
BspANI GGCC 1 cut(s) 686
BspCNI CTCAG 1 cut(s) 459
BspLI GGNNCC 5 cut(s) 580, 739, 830, 892, 995
BspMI ACCTGC 2 cut(s) 298, 1009
BspPI GGATC 1 cut(s) 739
BsrI ACTGG 2 cut(s) 733, 995
BssECI CCNNGG 2 cut(s) 99, 758
BssMI GATC 2 cut(s) 160, 731
BssT1I CCWWGG 2 cut(s) 99, 758
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 3 cut(s) 862, 933, 955
Bst6I CTCTTC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 467
BstDSI CCRYGG 2 cut(s) 99, 758
BstF5I GGATG 6 cut(s) 72, 398, 506, 722, 830, 946
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 2 cut(s) 605, 670
BstKTI GATC 2 cut(s) 163, 734
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 2 cut(s) 160, 731
BstMWI GCNNNNNNNGC 2 cut(s) 455, 485
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 2 cut(s) 259, 892
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 3 cut(s) 369, 760, 949
BstXI CCANNNNNNTGG 1 cut(s) 748
BsuRI GGCC 1 cut(s) 686
BtgI CCRYGG 2 cut(s) 99, 758
BtgZI GCGATG 1 cut(s) 369
BtrI CACGTC 1 cut(s) 455
BtsCI GGATG 6 cut(s) 72, 398, 506, 722, 830, 946
BtsIMutI CAGTG 1 cut(s) 464
BveI ACCTGC 2 cut(s) 298, 1009
CfoI GCGC 2 cut(s) 605, 670
Cfr13I GGNCC 1 cut(s) 890
Csp6I GTAC 1 cut(s) 517
CviAII CATG 2 cut(s) 100, 759
CviJI RGCY 9 cut(s) 104, 259, 329, 581, 686, 702, 767, 950, 994
CviKI_1 RGCY 9 cut(s) 104, 259, 329, 581, 686, 702, 767, 950, 994
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 1 cut(s) 467
DpnI GATC 2 cut(s) 162, 733
DpnII GATC 2 cut(s) 160, 731
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
EciI GGCGGA 1 cut(s) 494
Eco130I CCWWGG 2 cut(s) 99, 758
Eco47I GGWCC 1 cut(s) 890
Eco47III AGCGCT 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 99, 758
ErhI CCWWGG 2 cut(s) 99, 758
FaeI CATG 2 cut(s) 103, 762
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 4 cut(s) 18, 334, 465, 876
FatI CATG 2 cut(s) 99, 758
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 1 cut(s) 347
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 3 cut(s) 477, 489, 982
FokI GGATG 6 cut(s) 59, 385, 513, 729, 837, 953
Fsp4HI GCNGC 3 cut(s) 477, 489, 982
FspBI CTAG 1 cut(s) 774
GlaI GCGC 2 cut(s) 604, 669
GluI GCNGC 3 cut(s) 477, 489, 982
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 1 cut(s) 686
HapII CCGG 1 cut(s) 894
HhaI GCGC 2 cut(s) 605, 670
Hin1II CATG 2 cut(s) 103, 762
Hin6I GCGC 2 cut(s) 603, 668
HinP1I GCGC 2 cut(s) 603, 668
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 4 cut(s) 190, 206, 374, 842
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 1 cut(s) 894
HphI GGTGA 3 cut(s) 295, 369, 884
Hpy166II GTNNAC 1 cut(s) 726
Hpy188I TCNGA 4 cut(s) 403, 432, 940, 998
Hpy188III TCNNGA 4 cut(s) 194, 559, 961, 1018
Hpy8I GTNNAC 1 cut(s) 726
Hpy99I CGWCG 3 cut(s) 517, 685, 815
HpyAV CCTTC 3 cut(s) 569, 805, 1017
HpyCH4III ACNGT 3 cut(s) 862, 933, 955
HpyCH4IV ACGT 4 cut(s) 28, 454, 512, 680
HpyCH4V TGCA 4 cut(s) 143, 398, 464, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 485
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 4 cut(s) 28, 454, 512, 680
Hsp92II CATG 2 cut(s) 103, 762
HspAI GCGC 2 cut(s) 603, 668
Ksp22I TGATCA 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 2 cut(s) 160, 731
LmnI GCTCC 6 cut(s) 351, 578, 764, 896, 999, 1030
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 3 cut(s) 407, 491, 707
MaeI CTAG 1 cut(s) 774
MaeII ACGT 4 cut(s) 28, 454, 512, 680
MalI GATC 2 cut(s) 162, 733
MboI GATC 2 cut(s) 160, 731
MfeI CAATTG 2 cut(s) 33, 201
MluCI AATT 6 cut(s) 33, 144, 201, 264, 1050, 1054
MmeI TCCRAC 2 cut(s) 357, 1021
MnlI CCTC 4 cut(s) 86, 225, 462, 828
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 255, 725, 1053, 1057
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 2 cut(s) 261, 894
MunI CAATTG 2 cut(s) 33, 201
Mva1269I GAATGC 1 cut(s) 1027
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 455, 485
NciI CCSGG 1 cut(s) 894
NcoI CCATGG 2 cut(s) 99, 758
NdeI CATATG 1 cut(s) 347
NdeII GATC 2 cut(s) 160, 731
NlaIII CATG 2 cut(s) 103, 762
NlaIV GGNNCC 5 cut(s) 580, 739, 830, 892, 995
PacI TTAATTAA 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 1 cut(s) 644
PctI GAATGC 1 cut(s) 1027
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 4 cut(s) 190, 206, 374, 842
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 3 cut(s) 478, 490, 983
PshBI ATTAAT 1 cut(s) 1053
PsiI TTATAA 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 5 cut(s) 580, 739, 830, 892, 995
PspPI GGNCC 1 cut(s) 890
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
SaqAI TTAA 4 cut(s) 255, 725, 1053, 1057
SatI GCNGC 3 cut(s) 477, 489, 982
Sau3AI GATC 2 cut(s) 160, 731
Sau96I GGNCC 1 cut(s) 890
ScrFI CCNGG 2 cut(s) 261, 894
SfaNI GCATC 3 cut(s) 407, 491, 707
SinI GGWCC 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 819, 961
SmoI CTYRAG 2 cut(s) 819, 961
Sse9I AATT 6 cut(s) 33, 144, 201, 264, 1050, 1054
SsiI CCGC 6 cut(s) 293, 449, 458, 476, 479, 981
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 774
StyD4I CCNGG 2 cut(s) 259, 892
StyI CCWWGG 2 cut(s) 99, 758
TaaI ACNGT 3 cut(s) 862, 933, 955
TaiI ACGT 4 cut(s) 31, 457, 515, 683
TaqI TCGA 1 cut(s) 137
TasI AATT 6 cut(s) 33, 144, 201, 264, 1050, 1054
TauI GCSGC 2 cut(s) 479, 984
TfiI GAWTC 4 cut(s) 190, 206, 374, 842
Tru1I TTAA 4 cut(s) 255, 725, 1053, 1057
Tru9I TTAA 4 cut(s) 255, 725, 1053, 1057
TscAI CASTG 1 cut(s) 471
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 5 cut(s) 61, 235, 580, 633, 957
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 890
VspI ATTAAT 1 cut(s) 1053
XapI RAATTY 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.