Rh5CG472300

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003654
Physical Location & Seq
Reverse (-)
65813309 .. 65814651
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG472300.1

Sequence Viewer

Length: 1008 bp
ATGGTTCGTGGTACTTTTATGATGAACTTGGTATTCATAGCTTTGTATGCTTTCATGGCTATTTCAAATTCTCAAGCCGAAGGCACGAGTACTTCAGCGATTCTCATAACAGTTGACCAATCGGGCAAAGGTGATTACAAGAAAATACAAGAAGCCATTGATTCAGTGGCGTCGAACAACGCAGAGCTCGTGTTTATTAGGGTTAAGCCCGGAGTATATGTAGAAAAAGTTATGGTTCCGGCGGACAAGCCGTTTATAACATTAAGTGGCACAAAGGCAGCTGACACGGTTATAACATGGAACGATAGTGGGGACATATTCGAGTCTCCAACTTTCTCTGTCCTGGCCTCTGATTTCATAGGGCGTTACCTCACAATTCAGAATACATATGGAGCTGGTGTGAAAGCGGTGGCATTACGGGTGTCTGGAGATAGAGCAGCATTCTATGGGTGCAGAATTTTGTCTCATCAAGACACCCTCCTAGATGATACTGGGAGGCACTACTACAACAACTGTTACATTGAAGGAGATGTAGACTTCATATGTGGAAATGCTGCTTCTTTCTTTGAGAAGTGCCATTTGCATTCTCTTTCGGTAGGAACCGGGGCCATAACGGCCCAACGGAGAATGTCCCCAGCTGAGGACACCGGGTTCATGTTCTTCGGGTGCAAGATCACCGGTGTCAAAACAGCAGTGTTGGGAAGGCCTTGGGGTACATATTCAAGAGTGGTCTTTGCCTTCACTTACATGACTAGCGCCATACTGCCACAAGGTTGGGACGATTGGGGAAACTCAGCCGACCAATTAAGAACCGTATACTATGGGCAGTATAAATGTTCTGGACGGGGAGCAACTACATCGAAACGAGTAGAATGGGCACGCGACCTTACGAGCGAAGAAGTCGAACCCTTCTTGACTAAGGACATGATTGATGCCAATGCTTGGTTAAGGTCAGCACCAACCAGTTTCAAGAAGAAAGCACTCAGTGCCAATGACACCAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

335

Amino Acids

36.82

Weight (kDa)

6.89

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 36 - 312 2.1e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 257, 293
AccB7I CCANNNNNTGG 1 cut(s) 942
AccI GTMKAC 2 cut(s) 534, 816
AccII CGCG 1 cut(s) 882
AciI CCGC 2 cut(s) 242, 407
AcsI RAATTY 2 cut(s) 67, 456
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 170
AdeI CACNNNGTG 1 cut(s) 986
AfaI GTAC 3 cut(s) 13, 91, 715
AfiI CCNNNNNNNGG 2 cut(s) 640, 942
AgeI ACCGGT 1 cut(s) 677
AgsI TTSAA 4 cut(s) 66, 524, 723, 970
AjnI CCWGG 1 cut(s) 342
AloI GAACNNNNNNTCC 2 cut(s) 635, 667
AluBI AGCT 5 cut(s) 41, 187, 281, 395, 638
AluI AGCT 5 cut(s) 41, 187, 281, 395, 638
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 330, 468
AoxI GGCC 4 cut(s) 345, 606, 615, 704
ApeKI GCWGC 3 cut(s) 278, 437, 554
ApoI RAATTY 2 cut(s) 67, 456
AsiGI ACCGGT 1 cut(s) 677
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 2 cut(s) 606, 616
AsuC2I CCSGG 3 cut(s) 210, 604, 649
AsuHPI GGTGA 2 cut(s) 143, 667
BaeGI GKGCMC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 189
BauI CACGAG 2 cut(s) 85, 188
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 3 cut(s) 290, 449, 541
BceAI ACGGC 2 cut(s) 235, 630
BcgI CGANNNNNNTGC 2 cut(s) 840, 874
BciT130I CCWGG 1 cut(s) 344
BcnI CCSGG 3 cut(s) 210, 604, 649
BcoDI GTCTC 2 cut(s) 330, 468
BfaI CTAG 2 cut(s) 482, 753
BfoI RGCGCY 1 cut(s) 759
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 3 cut(s) 279, 438, 555
BlsI GCNGC 3 cut(s) 280, 439, 556
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 4 cut(s) 210, 344, 604, 649
BmgT120I GGNCC 2 cut(s) 606, 616
BmiI GGNNCC 3 cut(s) 237, 601, 607
BmrFI CCNGG 4 cut(s) 210, 344, 604, 649
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 922
BmuI ACTGGG 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 3 cut(s) 210, 604, 649
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 603, 707
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 640, 942
Bse118I RCCGGY 1 cut(s) 677
Bse1I ACTGG 2 cut(s) 496, 963
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 603, 707
BseLI CCNNNNNNNGG 2 cut(s) 640, 942
BseMII CTCAG 3 cut(s) 630, 807, 997
BseNI ACTGG 2 cut(s) 496, 963
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 3 cut(s) 290, 449, 541
BseYI CCCAGC 1 cut(s) 634
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 347, 608, 617, 706
BshTI ACCGGT 1 cut(s) 677
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 5 cut(s) 210, 239, 603, 648, 678
BslFI GGGAC 3 cut(s) 326, 616, 791
BslI CCNNNNNNNGG 2 cut(s) 640, 942
BsmAI GTCTC 2 cut(s) 330, 468
BsmFI GGGAC 3 cut(s) 326, 616, 791
BsmI GAATGC 2 cut(s) 440, 583
BsnI GGCC 4 cut(s) 347, 608, 617, 706
Bsp1286I GDGCHC 2 cut(s) 189, 880
Bsp143I GATC 1 cut(s) 672
BspACI CCGC 2 cut(s) 242, 407
BspANI GGCC 4 cut(s) 347, 608, 617, 706
BspCNI CTCAG 3 cut(s) 631, 806, 996
BspFNI CGCG 1 cut(s) 882
BspLI GGNNCC 3 cut(s) 237, 601, 607
BsrFI RCCGGY 1 cut(s) 677
BsrI ACTGG 2 cut(s) 496, 963
BssAI RCCGGY 1 cut(s) 677
BssECI CCNNGG 2 cut(s) 603, 707
BssMI GATC 1 cut(s) 672
BssNAI GTATAC 1 cut(s) 817
BssNI GRCGYC 1 cut(s) 170
BssSI CACGAG 2 cut(s) 85, 188
BssT1I CCWWGG 1 cut(s) 707
Bst1107I GTATAC 1 cut(s) 817
Bst2BI CACGAG 2 cut(s) 85, 188
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 4 cut(s) 112, 289, 515, 814
BstACI GRCGYC 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 1 cut(s) 880
BstDEI CTNAG 4 cut(s) 639, 793, 918, 983
BstFNI CGCG 1 cut(s) 882
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 1 cut(s) 675
BstMAI GTCTC 2 cut(s) 330, 468
BstMBI GATC 1 cut(s) 672
BstMWI GCNNNNNNNGC 4 cut(s) 47, 56, 614, 986
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 208, 342, 602, 647
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 3 cut(s) 290, 449, 541
BstXI CCANNNNNNTGG 1 cut(s) 774
BstZ17I GTATAC 1 cut(s) 817
BsuRI GGCC 4 cut(s) 347, 608, 617, 706
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 3 cut(s) 171, 699, 991
Cac8I GCNNGC 1 cut(s) 880
CfoI GCGC 1 cut(s) 758
Cfr10I RCCGGY 1 cut(s) 677
Cfr13I GGNCC 2 cut(s) 606, 616
CseI GACGC 1 cut(s) 159
Csp6I GTAC 3 cut(s) 12, 90, 714
CspAI ACCGGT 1 cut(s) 677
CviAII CATG 5 cut(s) 55, 297, 655, 748, 925
CviQI GTAC 3 cut(s) 12, 90, 714
DdeI CTNAG 4 cut(s) 639, 793, 918, 983
DpnI GATC 1 cut(s) 674
DpnII GATC 1 cut(s) 672
DraIII CACNNNGTG 1 cut(s) 986
EciI GGCGGA 1 cut(s) 257
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 707
Eco147I AGGCCT 1 cut(s) 706
Eco24I GRGCYC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 707
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 5 cut(s) 58, 300, 658, 751, 928
FaqI GGGAC 3 cut(s) 326, 616, 791
FatI CATG 5 cut(s) 54, 296, 654, 747, 924
FauNDI CATATG 2 cut(s) 388, 542
FblI GTMKAC 2 cut(s) 534, 816
Fnu4HI GCNGC 3 cut(s) 279, 438, 555
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 279, 438, 555
FspBI CTAG 2 cut(s) 482, 753
GlaI GCGC 1 cut(s) 757
GluI GCNGC 3 cut(s) 279, 438, 555
GsaI CCCAGC 1 cut(s) 638
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 759
HaeIII GGCC 4 cut(s) 347, 608, 617, 706
HapII CCGG 5 cut(s) 210, 239, 603, 648, 678
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 758
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 5 cut(s) 58, 300, 658, 751, 928
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 100, 161, 323
HpaII CCGG 5 cut(s) 210, 239, 603, 648, 678
HphI GGTGA 2 cut(s) 143, 667
Hpy166II GTNNAC 3 cut(s) 115, 535, 817
Hpy188I TCNGA 2 cut(s) 352, 381
Hpy188III TCNNGA 6 cut(s) 426, 470, 723, 840, 913, 970
Hpy8I GTNNAC 3 cut(s) 115, 535, 817
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 5 cut(s) 74, 518, 696, 748, 919
HpyCH4III ACNGT 4 cut(s) 112, 289, 515, 814
HpyCH4V TGCA 3 cut(s) 453, 583, 669
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 56, 614, 986
HpyF3I CTNAG 4 cut(s) 639, 793, 918, 983
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 5 cut(s) 58, 300, 658, 751, 928
HspAI GCGC 1 cut(s) 756
Kzo9I GATC 1 cut(s) 672
LmnI GCTCC 2 cut(s) 392, 848
Lsp1109I GCAGC 3 cut(s) 290, 449, 541
LweI GCATC 1 cut(s) 922
MaeI CTAG 2 cut(s) 482, 753
MaeIII GTNAC 2 cut(s) 365, 515
MalI GATC 1 cut(s) 674
MboI GATC 1 cut(s) 672
MboII GAAGA 3 cut(s) 652, 908, 985
MhlI GDGCHC 2 cut(s) 189, 880
MluCI AATT 5 cut(s) 67, 375, 456, 803, 1003
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 5 cut(s) 358, 380, 488, 489, 634
MseI TTAA 5 cut(s) 204, 263, 806, 947, 1006
MslI CAYNNNNRTG 1 cut(s) 746
MspA1I CMGCKG 2 cut(s) 281, 638
MspI CCGG 5 cut(s) 210, 239, 603, 648, 678
MspR9I CCNGG 4 cut(s) 210, 344, 604, 649
Mva1269I GAATGC 2 cut(s) 440, 583
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 4 cut(s) 47, 56, 614, 986
NciI CCSGG 3 cut(s) 210, 604, 649
NdeI CATATG 2 cut(s) 388, 542
NdeII GATC 1 cut(s) 672
NlaIII CATG 5 cut(s) 58, 300, 658, 751, 928
NlaIV GGNNCC 3 cut(s) 237, 601, 607
PceI AGGCCT 1 cut(s) 706
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 2 cut(s) 440, 583
PfeI GAWTC 2 cut(s) 100, 161
PflMI CCANNNNNTGG 1 cut(s) 942
PinAI ACCGGT 1 cut(s) 677
PkrI GCNGC 3 cut(s) 280, 439, 556
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PsiI TTATAA 2 cut(s) 257, 293
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 3 cut(s) 237, 601, 607
PspPI GGNCC 2 cut(s) 606, 616
PvuII CAGCTG 2 cut(s) 281, 638
RsaI GTAC 3 cut(s) 13, 91, 715
RsaNI GTAC 3 cut(s) 12, 90, 714
RseI CAYNNNNRTG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 5 cut(s) 204, 263, 806, 947, 1006
SatI GCNGC 3 cut(s) 279, 438, 555
Sau3AI GATC 1 cut(s) 672
Sau96I GGNCC 2 cut(s) 606, 616
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 4 cut(s) 210, 344, 604, 649
SduI GDGCHC 2 cut(s) 189, 880
SfaNI GCATC 1 cut(s) 922
SfiI GGCCNNNNNGGCC 1 cut(s) 614
SgrAI CRCCGGYG 1 cut(s) 677
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 5 cut(s) 67, 375, 456, 803, 1003
SseBI AGGCCT 1 cut(s) 706
SsiI CCGC 2 cut(s) 242, 407
SspMI CTAG 2 cut(s) 482, 753
SstI GAGCTC 1 cut(s) 189
StuI AGGCCT 1 cut(s) 706
StyD4I CCNGG 4 cut(s) 208, 342, 602, 647
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 4 cut(s) 112, 289, 515, 814
TaqI TCGA 4 cut(s) 173, 321, 860, 903
TasI AATT 5 cut(s) 67, 375, 456, 803, 1003
TatI WGTACW 1 cut(s) 89
TfiI GAWTC 2 cut(s) 100, 161
Tru1I TTAA 5 cut(s) 204, 263, 806, 947, 1006
Tru9I TTAA 5 cut(s) 204, 263, 806, 947, 1006
TscAI CASTG 3 cut(s) 171, 699, 991
TseI GCWGC 3 cut(s) 278, 437, 554
TspDTI ATGAA 6 cut(s) 25, 38, 43, 346, 529, 643
TspGWI ACGGA 1 cut(s) 637
TspRI CASTG 3 cut(s) 171, 699, 991
Van91I CCANNNNNTGG 1 cut(s) 942
XapI RAATTY 2 cut(s) 67, 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmiI GTMKAC 2 cut(s) 534, 816
XspI CTAG 2 cut(s) 482, 753
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.