RchiOBHm_Chr6g0301011

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003654
Physical Location & Seq
Reverse (-)
61597007 .. 61598461
1455 bp
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UTR
Exon/CDS
Intron
PRQ27032

Sequence Viewer

Length: 1455 bp
ATGGCGCGTAGGGCTCTAGTTAGAATGATTGTCATAATTTTGTATGCTTTCGTCACTATTTCTCAAGCGGAAAATATAATAACAGTTGATCAATCCGGTAACGGTCATTACGGTAAGATACAAGATGCTATTGATTCTGTTCCGTCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGGACTTACAAAGAAAGTATTACCATACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAGAAGCAAGTGATGTGATGATTACGTGGGATCAGAGAGGGGACATTGATAAAGTTACAACATTTCATGTTTCGGCTTCTGACTTCGTAGCGCACTACATAACAATTCAAAATACATTCGGGCCTGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAATAGCGTTTTACGACTGCTCAATTTTAGGTTATCAGGATACTCTATTTGACGAAATAGGCAAACACTACTATGAAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCGCAACGAAGAGAAGCGTCAACAGATAGTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAAAGGCGAAAAAACATTTTTAGGAAGACCATGGGTGGGATCCTATGCTAGGGTCATATTTGCCTACTCTAACATGCAAGATGTGATAGAACCACTAGGTTGGTCCCGTGATTCTGATGGATCCACAGAACAAGAATTAATGACAAACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAAACTTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAGGATTGGATAAAGTCTACGCCGGTAACAACGTCGCCTCCACCTAAAAAGGAGACGCCGTCATTGCCTCCATCGGTACTGGATACACCACCACCAGCACCGGTCATCCCGGCACCTGTAATCCCGTCTCCACCACCACAAGCCCCGGTGGTACTAACTTCCCCACCACCGGTGATTCCATTAGTTTCCCCACCAGCACCGGTGGCGATCCCGTCGTCGACACCACTGGTAAATCCATTTTCACCACCACCGGTACCGATAATTCCGTTGTTGCCCCCACGGGCACCGGTGGTGATCTTCTCACCAGCGACACCCGTGATCTGGTGGTTCGCACCACCGCCACGAGAGAGGGTGATTATAACTCCGTGGTCGCCTCCACCAACATACATGGACGGCAGAGCTCAAGAAGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGCATCCTCTTCAATCTTTAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTAACCTCGGCAGTTTTACTATACTTGTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

484

Amino Acids

52.74

Weight (kDa)

5.84

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 308 8.5e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1283
Acc65I GGTACC 1 cut(s) 1177
AccB1I GGYRCC 3 cut(s) 1036, 1177, 1207
AccI GTMKAC 2 cut(s) 941, 1142
AccII CGCG 2 cut(s) 7, 868
AciI CCGC 3 cut(s) 68, 1262, 1401
AclWI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1126
AcsI RAATTY 1 cut(s) 895
AcuI CTGAAG 1 cut(s) 859
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 4 cut(s) 244, 1002, 1077, 1179
AfiI CCNNNNNNNGG 5 cut(s) 575, 689, 702, 1093, 1245
AgeI ACCGGT 5 cut(s) 1024, 1093, 1123, 1174, 1210
AgsI TTSAA 5 cut(s) 359, 503, 548, 820, 1380
AjnI CCWGG 2 cut(s) 181, 373
AluBI AGCT 1 cut(s) 1325
AluI AGCT 1 cut(s) 1325
Alw21I GWGCWC 1 cut(s) 1327
Alw26I GTCTC 3 cut(s) 406, 971, 1056
AlwI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1126
Ama87I CYCGRG 1 cut(s) 1363
AoxI GGCC 2 cut(s) 371, 1449
ApoI RAATTY 1 cut(s) 895
AseI ATTAAT 1 cut(s) 791
AsiGI ACCGGT 5 cut(s) 1024, 1093, 1123, 1174, 1210
Asp700I GAANNNNTTC 1 cut(s) 1394
Asp718I GGTACC 1 cut(s) 1177
AspLEI GCGC 6 cut(s) 7, 343, 394, 533, 592, 868
AspS9I GGNCC 2 cut(s) 371, 756
AsuC2I CCSGG 2 cut(s) 1034, 1070
AsuHPI GGTGA 6 cut(s) 1108, 1158, 1218, 1228, 1288, 1346
AvaI CYCGRG 1 cut(s) 1363
AvaII GGWCC 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 1210
BamHI GGATCC 3 cut(s) 157, 692, 773
BanI GGYRCC 3 cut(s) 1036, 1177, 1207
BanII GRGCYC 2 cut(s) 16, 1327
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 1327
BccI CCATC 2 cut(s) 764, 1003
BceAI ACGGC 2 cut(s) 967, 1333
BciT130I CCWGG 2 cut(s) 183, 375
BciVI GTATCC 2 cut(s) 445, 1000
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 1034, 1070
BcoDI GTCTC 3 cut(s) 406, 971, 1056
BfaI CTAG 4 cut(s) 17, 702, 749, 833
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 2 cut(s) 445, 1000
BisI GCNGC 1 cut(s) 1402
BlsI GCNGC 1 cut(s) 1403
Bme1390I CCNGG 4 cut(s) 183, 375, 1034, 1070
Bme18I GGWCC 1 cut(s) 756
BmeT110I CYCGRG 1 cut(s) 1363
BmgT120I GGNCC 2 cut(s) 371, 756
BmiI GGNNCC 7 cut(s) 159, 694, 758, 775, 1038, 1179, 1209
BmrFI CCNGG 4 cut(s) 183, 375, 1034, 1070
BmsI GCATC 2 cut(s) 115, 1379
BpiI GAAGAC 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 48, 1311
BpuMI CCSGG 2 cut(s) 1034, 1070
BsaAI YACGTR 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 980
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 7 cut(s) 182, 683, 1068, 1202, 1289, 1362, 1425
BsaWI WCCGGW 6 cut(s) 95, 1024, 1093, 1123, 1174, 1210
BsaXI ACNNNNNCTCC 8 cut(s) 196, 226, 612, 642, 946, 968, 976, 998
Bsc4I CCNNNNNNNGG 5 cut(s) 575, 689, 702, 1093, 1245
Bse118I RCCGGY 6 cut(s) 946, 1024, 1093, 1123, 1174, 1210
Bse1I ACTGG 4 cut(s) 534, 655, 1008, 1155
Bse3DI GCAATG 1 cut(s) 986
BseBI CCWGG 2 cut(s) 183, 375
BseDI CCNNGG 7 cut(s) 182, 683, 1068, 1202, 1289, 1362, 1425
BseGI GGATG 3 cut(s) 845, 1029, 1370
BseLI CCNNNNNNNGG 5 cut(s) 575, 689, 702, 1093, 1245
BseMI GCAATG 1 cut(s) 986
BseNI ACTGG 4 cut(s) 534, 655, 1008, 1155
BseSI GKGCMC 1 cut(s) 1210
Bsh1236I CGCG 2 cut(s) 7, 868
BshFI GGCC 2 cut(s) 373, 1451
BshNI GGYRCC 3 cut(s) 1036, 1177, 1207
BshTI ACCGGT 5 cut(s) 1024, 1093, 1123, 1174, 1210
BsiHKAI GWGCWC 1 cut(s) 1327
BsiHKCI CYCGRG 1 cut(s) 1363
BslFI GGGAC 4 cut(s) 199, 305, 742, 1423
BslI CCNNNNNNNGG 5 cut(s) 575, 689, 702, 1093, 1245
BsmAI GTCTC 3 cut(s) 406, 971, 1056
BsmBI CGTCTC 2 cut(s) 971, 1056
BsmFI GGGAC 4 cut(s) 199, 305, 742, 1423
BsnI GGCC 2 cut(s) 373, 1451
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 1 cut(s) 1363
Bsp1286I GDGCHC 3 cut(s) 16, 1210, 1327
Bsp143I GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 3 cut(s) 68, 1262, 1401
BspANI GGCC 2 cut(s) 373, 1451
BspFNI CGCG 2 cut(s) 7, 868
BspLI GGNNCC 7 cut(s) 159, 694, 758, 775, 1038, 1179, 1209
BspPI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1126
BspT107I GGYRCC 3 cut(s) 1036, 1177, 1207
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 986
BsrFI RCCGGY 6 cut(s) 946, 1024, 1093, 1123, 1174, 1210
BsrI ACTGG 4 cut(s) 534, 655, 1008, 1155
BssAI RCCGGY 6 cut(s) 946, 1024, 1093, 1123, 1174, 1210
BssECI CCNNGG 7 cut(s) 182, 683, 1068, 1202, 1289, 1362, 1425
BssMI GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
BssNI GRCGYC 1 cut(s) 980
BssSI CACGAG 1 cut(s) 1266
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 2 cut(s) 183, 375
Bst4CI ACNGT 3 cut(s) 85, 104, 113
Bst6I CTCTTC 2 cut(s) 592, 1381
BstACI GRCGYC 1 cut(s) 980
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 1416
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 3 cut(s) 683, 1202, 1289
BstENI CCTNNNNNAGG 2 cut(s) 573, 700
BstF5I GGATG 3 cut(s) 845, 1029, 1370
BstFNI CGCG 2 cut(s) 7, 868
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 6 cut(s) 7, 343, 394, 533, 592, 868
BstKTI GATC 9 cut(s) 91, 160, 177, 283, 695, 776, 1134, 1221, 1245
BstMAI GTCTC 3 cut(s) 406, 971, 1056
BstMBI GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
BstMWI GCNNNNNNNGC 3 cut(s) 11, 988, 1127
BstNI CCWGG 2 cut(s) 183, 375
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 4 cut(s) 181, 373, 1032, 1068
BstSFI CTRYAG 2 cut(s) 249, 570
BstSLI GKGCMC 1 cut(s) 1210
BstUI CGCG 2 cut(s) 7, 868
BstV2I GAAGAC 1 cut(s) 685
BstX2I RGATCY 3 cut(s) 157, 692, 773
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 3 cut(s) 157, 692, 773
BsuI GTATCC 2 cut(s) 445, 1000
BsuRI GGCC 2 cut(s) 373, 1451
BtgI CCRYGG 3 cut(s) 683, 1202, 1289
BtsCI GGATG 3 cut(s) 845, 1029, 1370
BtsIMutI CAGTG 2 cut(s) 883, 1148
Cac8I GCNNGC 1 cut(s) 1416
CfoI GCGC 6 cut(s) 7, 343, 394, 533, 592, 868
Cfr10I RCCGGY 6 cut(s) 946, 1024, 1093, 1123, 1174, 1210
Cfr13I GGNCC 2 cut(s) 371, 756
CseI GACGC 3 cut(s) 594, 988, 1420
Csp6I GTAC 4 cut(s) 243, 1001, 1076, 1178
CspAI ACCGGT 5 cut(s) 1024, 1093, 1123, 1174, 1210
CviAII CATG 5 cut(s) 317, 684, 727, 1312, 1348
CviJI RGCY 9 cut(s) 14, 181, 223, 326, 373, 1067, 1325, 1418, 1451
CviKI_1 RGCY 9 cut(s) 14, 181, 223, 326, 373, 1067, 1325, 1418, 1451
CviQI GTAC 4 cut(s) 243, 1001, 1076, 1178
DdeI CTNAG 1 cut(s) 395
DpnI GATC 9 cut(s) 90, 159, 176, 282, 694, 775, 1133, 1220, 1244
DpnII GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
Eam1104I CTCTTC 2 cut(s) 592, 1381
EarI CTCTTC 2 cut(s) 592, 1381
Ecl136II GAGCTC 1 cut(s) 1325
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 16, 1327
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 756
Eco53kI GAGCTC 1 cut(s) 1325
Eco57I CTGAAG 1 cut(s) 859
Eco88I CYCGRG 1 cut(s) 1363
EcoICRI GAGCTC 1 cut(s) 1325
EcoNI CCTNNNNNAGG 2 cut(s) 573, 700
EcoRII CCWGG 2 cut(s) 181, 373
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 16, 1327
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 2 cut(s) 971, 1056
FaeI CATG 5 cut(s) 320, 687, 730, 1315, 1351
FaqI GGGAC 4 cut(s) 199, 305, 742, 1423
FatI CATG 5 cut(s) 316, 683, 726, 1311, 1347
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 2 cut(s) 941, 1142
Fnu4HI GCNGC 1 cut(s) 1402
FokI GGATG 3 cut(s) 832, 1016, 1357
FriOI GRGCYC 2 cut(s) 16, 1327
Fsp4HI GCNGC 1 cut(s) 1402
FspBI CTAG 4 cut(s) 17, 702, 749, 833
GlaI GCGC 6 cut(s) 6, 342, 393, 532, 591, 867
GluI GCNGC 1 cut(s) 1402
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 2 cut(s) 373, 1451
HgaI GACGC 3 cut(s) 594, 988, 1420
HhaI GCGC 6 cut(s) 7, 343, 394, 533, 592, 868
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 5 cut(s) 320, 687, 730, 1315, 1351
Hin6I GCGC 6 cut(s) 5, 341, 392, 531, 590, 866
HinP1I GCGC 6 cut(s) 5, 341, 392, 531, 590, 866
HincII GTYRAC 2 cut(s) 609, 1143
HindII GTYRAC 2 cut(s) 609, 1143
HinfI GANTC 3 cut(s) 134, 764, 1099
HphI GGTGA 6 cut(s) 1108, 1158, 1218, 1228, 1288, 1346
Hpy166II GTNNAC 5 cut(s) 609, 812, 942, 1143, 1337
Hpy188I TCNGA 3 cut(s) 285, 331, 769
Hpy188III TCNNGA 3 cut(s) 215, 449, 1328
Hpy8I GTNNAC 5 cut(s) 609, 812, 942, 1143, 1337
Hpy99I CGWCG 4 cut(s) 830, 961, 1141, 1144
HpyAV CCTTC 2 cut(s) 497, 1325
HpyCH4III ACNGT 3 cut(s) 85, 104, 113
HpyCH4IV ACGT 2 cut(s) 275, 956
HpyCH4V TGCA 2 cut(s) 730, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 988, 1127
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 275, 956
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 5 cut(s) 320, 687, 730, 1315, 1351
HspAI GCGC 6 cut(s) 5, 341, 392, 531, 590, 866
KpnI GGTACC 1 cut(s) 1181
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
LmnI GCTCC 1 cut(s) 836
LweI GCATC 2 cut(s) 115, 1379
MaeI CTAG 4 cut(s) 17, 702, 749, 833
MaeII ACGT 2 cut(s) 275, 956
MaeIII GTNAC 6 cut(s) 52, 98, 304, 494, 554, 949
MalI GATC 9 cut(s) 90, 159, 176, 282, 694, 775, 1133, 1220, 1244
MboI GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
MboII GAAGA 4 cut(s) 609, 690, 1213, 1368
MflI RGATCY 3 cut(s) 157, 692, 773
MhlI GDGCHC 3 cut(s) 16, 1210, 1327
MmeI TCCRAC 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 1394
MseI TTAA 5 cut(s) 236, 636, 791, 858, 1386
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 1401
MspR9I CCNGG 4 cut(s) 183, 375, 1034, 1070
MvaI CCWGG 2 cut(s) 183, 375
MvnI CGCG 2 cut(s) 7, 868
MwoI GCNNNNNNNGC 3 cut(s) 11, 988, 1127
NciI CCSGG 2 cut(s) 1034, 1070
NcoI CCATGG 1 cut(s) 683
NdeII GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
NlaIII CATG 5 cut(s) 320, 687, 730, 1315, 1351
NlaIV GGNNCC 7 cut(s) 159, 694, 758, 775, 1038, 1179, 1209
NmeAIII GCCGAG 1 cut(s) 1406
NmuCI GTSAC 2 cut(s) 52, 554
NspI RCATGY 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 1394
PfeI GAWTC 3 cut(s) 134, 764, 1099
PflFI GACNNNGTC 1 cut(s) 982
PinAI ACCGGT 5 cut(s) 1024, 1093, 1123, 1174, 1210
PkrI GCNGC 1 cut(s) 1403
Ppu21I YACGTR 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1283
Psp124BI GAGCTC 1 cut(s) 1327
Psp6I CCWGG 2 cut(s) 181, 373
PspGI CCWGG 2 cut(s) 181, 373
PspN4I GGNNCC 7 cut(s) 159, 694, 758, 775, 1038, 1179, 1209
PspPI GGNCC 2 cut(s) 371, 756
PsuI RGATCY 3 cut(s) 157, 692, 773
PsyI GACNNNGTC 1 cut(s) 982
RsaI GTAC 4 cut(s) 244, 1002, 1077, 1179
RsaNI GTAC 4 cut(s) 243, 1001, 1076, 1178
RseI CAYNNNNRTG 1 cut(s) 483
SacI GAGCTC 1 cut(s) 1327
SalI GTCGAC 1 cut(s) 1141
SaqAI TTAA 5 cut(s) 236, 636, 791, 858, 1386
SatI GCNGC 1 cut(s) 1402
Sau3AI GATC 9 cut(s) 88, 157, 174, 280, 692, 773, 1131, 1218, 1242
Sau96I GGNCC 2 cut(s) 371, 756
ScrFI CCNGG 4 cut(s) 183, 375, 1034, 1070
SduI GDGCHC 3 cut(s) 16, 1210, 1327
SfaNI GCATC 2 cut(s) 115, 1379
SfcI CTRYAG 2 cut(s) 249, 570
SgrAI CRCCGGYG 3 cut(s) 1093, 1123, 1210
SinI GGWCC 1 cut(s) 756
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 2 cut(s) 63, 1326
SmoI CTYRAG 2 cut(s) 63, 1326
SsiI CCGC 3 cut(s) 68, 1262, 1401
SspMI CTAG 4 cut(s) 17, 702, 749, 833
SstI GAGCTC 1 cut(s) 1327
StyD4I CCNGG 4 cut(s) 181, 373, 1032, 1068
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 3 cut(s) 85, 104, 113
TaiI ACGT 2 cut(s) 278, 959
TaqI TCGA 2 cut(s) 173, 1142
TauI GCSGC 1 cut(s) 1404
TfiI GAWTC 3 cut(s) 134, 764, 1099
Tru1I TTAA 5 cut(s) 236, 636, 791, 858, 1386
Tru9I TTAA 5 cut(s) 236, 636, 791, 858, 1386
TscAI CASTG 2 cut(s) 883, 1155
TseFI GTSAC 2 cut(s) 52, 554
Tsp45I GTSAC 2 cut(s) 52, 554
TspDTI ATGAA 3 cut(s) 217, 305, 501
TspGWI ACGGA 4 cut(s) 132, 843, 1179, 1278
TspRI CASTG 2 cut(s) 883, 1155
Tth111I GACNNNGTC 1 cut(s) 982
VpaK11BI GGWCC 1 cut(s) 756
VspI ATTAAT 1 cut(s) 791
XagI CCTNNNNNAGG 2 cut(s) 573, 700
XapI RAATTY 1 cut(s) 895
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 941, 1142
XmnI GAANNNNTTC 1 cut(s) 1394
XspI CTAG 4 cut(s) 17, 702, 749, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.