MD15G1025200.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003654
Physical Location & Seq
Reverse (-)
1478498 .. 1479106
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1025200.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCTAAATTTCTGACCCGTATGATCAAGTGCTTCTTCTCCATGGCCTTATTACTTCCGACTTTTAGGCATGCGCTCTCCCAAGCAGCTCAAGTGAACATACCCCCGGCGCCCCCAACGGTTTTTATAACCGTCGACCAGTCTGGCAGCAACGATTACACTAAAATACAAGACGCCATCGATGCTGTACCGTCCAACAATGCCCTGCCTGTGTATATTTGGGTTAAACCTGGCATCTACTACGAAAAGATTAATGTACCTTCAGACAAACCGTTTATAACAATAAGTGGATCGACAAGTTATGCAAGCGACACCGTGATAACGTGGAAGGAATCCGGCGAGATAAATGAAACCGCAGTCTTCACTTTATGGGCATCTGATTTCGTAGGGCGCAACTTCACAGTTCAGAATACGTATGGGACCGGTGGCAAAGCAGTTGCACTGAGAGTCTCGGGAGACAGAGTAGCATTCTATGGTTGCAGCATTATGTCTCATCAAGATGCCTTATTTGCCGATATAGGCAAGCAATACTACAAAGACTGCTACATTGAAGGAGACACTGATTTTATATTTGGAAGCGCTTCTTCTTTATTTGAGGTATGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

203

Amino Acids

22.26

Weight (kDa)

5.09

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 43 - 201 2.3e-36 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 128, 278
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 135
AciI CCGC 1 cut(s) 354
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 246
AcyI GRCGYC 2 cut(s) 110, 174
AfaI GTAC 2 cut(s) 189, 258
AfeI AGCGCT 1 cut(s) 580
AgeI ACCGGT 1 cut(s) 422
AgsI TTSAA 1 cut(s) 551
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
Alw26I GTCTC 4 cut(s) 450, 454, 495, 549
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 451
Aor51HI AGCGCT 1 cut(s) 580
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 86, 147, 480
ApoI RAATTY 1 cut(s) 8
AseI ATTAAT 1 cut(s) 252
AsiGI ACCGGT 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 580
AspLEI GCGC 4 cut(s) 76, 112, 393, 581
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 107
AvaI CYCGRG 1 cut(s) 451
AvaII GGWCC 1 cut(s) 420
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 98, 159, 492
BccI CCATC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 231
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 4 cut(s) 450, 454, 495, 549
BfoI RGCGCY 2 cut(s) 113, 582
BisI GCNGC 3 cut(s) 87, 148, 481
BlsI GCNGC 3 cut(s) 88, 149, 482
Bme1390I CCNGG 2 cut(s) 107, 231
Bme18I GGWCC 1 cut(s) 420
BmeT110I CYCGRG 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 111, 421
BmrFI CCNGG 2 cut(s) 107, 231
BmsI GCATC 4 cut(s) 172, 243, 383, 490
BpiI GAAGAC 1 cut(s) 352
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 414
BsaHI GRCGYC 2 cut(s) 110, 174
BsaJI CCNNGG 2 cut(s) 42, 105
BsaWI WCCGGW 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 42, 105
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 98, 159, 492
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 109
BshTI ACCGGT 1 cut(s) 422
BshVI ATCGAT 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 3 cut(s) 107, 336, 423
BslFI GGGAC 1 cut(s) 433
BsmAI GTCTC 4 cut(s) 450, 454, 495, 549
BsmFI GGGAC 1 cut(s) 433
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 451
Bsp143I GATC 2 cut(s) 24, 290
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 1 cut(s) 354
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 435
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 111, 421
BspPI GGATC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 42, 105
BssMI GATC 2 cut(s) 24, 290
BssNI GRCGYC 2 cut(s) 110, 174
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 6 cut(s) 121, 133, 192, 273, 316, 403
BstACI GRCGYC 2 cut(s) 110, 174
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 72, 307, 524
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 42
BstH2I RGCGCY 2 cut(s) 113, 582
BstHHI GCGC 4 cut(s) 76, 112, 393, 581
BstKTI GATC 2 cut(s) 27, 293
BstMAI GTCTC 4 cut(s) 450, 454, 495, 549
BstMBI GATC 2 cut(s) 24, 290
BstMWI GCNNNNNNNGC 2 cut(s) 182, 509
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 105, 229
BstSNI TACGTA 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 98, 159, 492
BstV2I GAAGAC 1 cut(s) 352
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 47
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 440, 558
Cac8I GCNNGC 3 cut(s) 72, 307, 524
CfoI GCGC 4 cut(s) 76, 112, 393, 581
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 420
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 188, 257
CspAI ACCGGT 1 cut(s) 422
CviAII CATG 2 cut(s) 43, 71
CviJI RGCY 3 cut(s) 5, 47, 89
CviKI_1 RGCY 3 cut(s) 5, 47, 89
CviQI GTAC 2 cut(s) 188, 257
DdeI CTNAG 1 cut(s) 443
DinI GGCGCC 1 cut(s) 111
DpnI GATC 2 cut(s) 26, 292
DpnII GATC 2 cut(s) 24, 290
Eco105I TACGTA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 420
Eco47III AGCGCT 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 42
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 2 cut(s) 46, 74
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 568, 600
FaqI GGGAC 1 cut(s) 433
FatI CATG 2 cut(s) 42, 70
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 87, 148, 481
Fsp4HI GCNGC 3 cut(s) 87, 148, 481
GlaI GCGC 4 cut(s) 75, 111, 392, 580
GluI GCNGC 3 cut(s) 87, 148, 481
HaeII RGCGCY 2 cut(s) 113, 582
HaeIII GGCC 1 cut(s) 47
HapII CCGG 3 cut(s) 107, 336, 423
HgaI GACGC 1 cut(s) 182
HhaI GCGC 4 cut(s) 76, 112, 393, 581
Hin1I GRCGYC 2 cut(s) 110, 174
Hin1II CATG 2 cut(s) 46, 74
Hin6I GCGC 4 cut(s) 74, 110, 391, 579
HinP1I GCGC 4 cut(s) 74, 110, 391, 579
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 2 cut(s) 332, 447
HpaII CCGG 3 cut(s) 107, 336, 423
Hpy166II GTNNAC 2 cut(s) 97, 136
Hpy188I TCNGA 5 cut(s) 15, 60, 265, 379, 408
Hpy188III TCNNGA 2 cut(s) 453, 497
Hpy8I GTNNAC 2 cut(s) 97, 136
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 3 cut(s) 270, 322, 545
HpyCH4III ACNGT 6 cut(s) 121, 133, 192, 273, 316, 403
HpyCH4IV ACGT 2 cut(s) 323, 413
HpyCH4V TGCA 3 cut(s) 305, 440, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 509
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 323, 413
Hsp92I GRCGYC 2 cut(s) 110, 174
Hsp92II CATG 2 cut(s) 46, 74
HspAI GCGC 4 cut(s) 74, 110, 391, 579
KasI GGCGCC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 2 cut(s) 24, 290
LpnPI CCDG 9 cut(s) 120, 129, 152, 216, 218, 222, 243, 349, 436
Lsp1109I GCAGC 3 cut(s) 98, 159, 492
LweI GCATC 4 cut(s) 172, 243, 383, 490
MaeII ACGT 2 cut(s) 323, 413
MalI GATC 2 cut(s) 26, 292
MboI GATC 2 cut(s) 24, 290
MboII GAAGA 3 cut(s) 28, 352, 576
MluCI AATT 1 cut(s) 8
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 2 cut(s) 83, 219
MnlI CCTC 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 2 cut(s) 225, 252
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 3 cut(s) 107, 336, 423
MspR9I CCNGG 2 cut(s) 107, 231
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 182, 509
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 107
NcoI CCATGG 1 cut(s) 42
NdeII GATC 2 cut(s) 24, 290
NlaIII CATG 2 cut(s) 46, 74
NlaIV GGNNCC 2 cut(s) 111, 421
NspI RCATGY 1 cut(s) 74
PaeI GCATGC 1 cut(s) 74
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 422
PkrI GCNGC 3 cut(s) 88, 149, 482
PleI GAGTC 1 cut(s) 455
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 455
Ppu21I YACGTR 1 cut(s) 414
PshBI ATTAAT 1 cut(s) 252
PsiI TTATAA 2 cut(s) 128, 278
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 111, 421
PspPI GGNCC 1 cut(s) 420
RsaI GTAC 2 cut(s) 189, 258
RsaNI GTAC 2 cut(s) 188, 257
RseI CAYNNNNRTG 1 cut(s) 498
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 225, 252
SatI GCNGC 3 cut(s) 87, 148, 481
Sau3AI GATC 2 cut(s) 24, 290
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 2 cut(s) 107, 231
SetI ASST 6 cut(s) 91, 232, 262, 326, 416, 600
SfaNI GCATC 4 cut(s) 172, 243, 383, 490
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
SnaBI TACGTA 1 cut(s) 414
SphI GCATGC 1 cut(s) 74
Sse9I AATT 1 cut(s) 8
SsiI CCGC 1 cut(s) 354
SspDI GGCGCC 1 cut(s) 109
StyD4I CCNGG 2 cut(s) 105, 229
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 6 cut(s) 121, 133, 192, 273, 316, 403
TaiI ACGT 2 cut(s) 326, 416
TaqI TCGA 3 cut(s) 135, 180, 293
TasI AATT 1 cut(s) 8
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 2 cut(s) 225, 252
Tru9I TTAA 2 cut(s) 225, 252
TscAI CASTG 2 cut(s) 447, 565
TseI GCWGC 3 cut(s) 86, 147, 480
TspDTI ATGAA 1 cut(s) 363
TspRI CASTG 2 cut(s) 447, 565
VpaK11BI GGWCC 1 cut(s) 420
VspI ATTAAT 1 cut(s) 252
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.