MD15G1025100.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003654
Physical Location & Seq
Reverse (-)
1477220 .. 1478425
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1025100.v1.1.491

Sequence Viewer

Length: 969 bp
ATGCATTCGCTTTCGGAACAAAACGGAGCTATAACAGCCCAAAAAAGGGATTCACCATCACAAGATGCAGGGTTTTTGTTCTTGGGAGGCGAAATCACTGGCGTGGGAAGGGCAGTATTAGGAAGGCCATGGGGTCCCTATTCGACAGTCATCTTTGCCTATACTAAGATGTCAAATGCGATTCTCCCACAAGGTTGGGACAGTTGGGGCCGATCAACTGACTACTTAAGTAAGGTTTACTATGGTGAGTACAAATGTATTGGAGCTGGAGCCGTGACAGCAGAAAGAGTTGCGTGGGAACACAACGTTACGCCAAAAGAATTTACATCCTTCATGAACAAGGTTAGGGGCTTTGGAATTGGAAGCTGGCAAGGTTCAGATGGAGGAGGTTGGAAATATGGAGCTCCCCCTCGTGTCGGAAGAGATGGAGAAGGAAACAATGGAGGACGGCAACAAGGACCTCCTACTCCTATTGGAGATGGAGGGAAACAAGGATATCCTACTCCTATTGGAGGTGGAGGGCAACATGGACCTCCTCCTCCCGCAACTAGAGAAGGAGGACAATATGGAGCTCCCGCTCCCTTTGCAGTAGGATGGCAACATGGACCTCCTCCTTCTGTTGCGGGAGGAGGACAATATGGAGCTCCCCCTCCATTTGCAGGAGGATGGCAACAAGGACCTCCTACTCCGGTTGGGGGAGGAGGGCAACATGGACCACCTCCTCCTGTAGGAGGAGAACGAGAAAGAAACAATGGAGGAGGATGGAAACATGCAGCACCCATTCCCAATGGTGGAGGAGGAGGAGTTAGAAACAATGCAGGAGTAGGGCAACATGGAGTTCCTAATGTAGGAGGAGCCTCCATCGAGGGGTGGGCTGATGTGCAATGGAGTGGTTGTGAAAAAGGTGCTGCCCATCCAGGCCTTTGGGGATTGTCTCTTGTTTTGCTTCCCTTAATCCACTTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

323

Amino Acids

32.96

Weight (kDa)

8.82

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 4 - 101 1.7e-19 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 578
AciI CCGC 3 cut(s) 543, 576, 623
AclI AACGTT 1 cut(s) 306
AcsI RAATTY 1 cut(s) 320
AfaI GTAC 1 cut(s) 251
AflII CTTAAG 1 cut(s) 226
AjnI CCWGG 1 cut(s) 916
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 6 cut(s) 29, 266, 366, 404, 572, 644
AluI AGCT 6 cut(s) 29, 266, 366, 404, 572, 644
Alw21I GWGCWC 3 cut(s) 406, 574, 646
Alw26I GTCTC 1 cut(s) 939
AoxI GGCC 3 cut(s) 125, 208, 919
ApeKI GCWGC 2 cut(s) 773, 908
ApoI RAATTY 1 cut(s) 320
AspS9I GGNCC 7 cut(s) 134, 208, 458, 530, 605, 677, 713
AsuHPI GGTGA 2 cut(s) 45, 257
AvaII GGWCC 6 cut(s) 134, 458, 530, 605, 677, 713
BanII GRGCYC 3 cut(s) 406, 574, 646
BauI CACGAG 1 cut(s) 411
Bbv12I GWGCWC 3 cut(s) 406, 574, 646
BbvI GCAGC 2 cut(s) 785, 895
BccI CCATC 9 cut(s) 64, 374, 419, 473, 588, 660, 756, 869, 921
BceAI ACGGC 2 cut(s) 257, 464
BciT130I CCWGG 1 cut(s) 918
BcoDI GTCTC 1 cut(s) 939
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 726
BfrI CTTAAG 1 cut(s) 226
BisI GCNGC 2 cut(s) 774, 909
BlsI GCNGC 2 cut(s) 775, 910
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 6 cut(s) 134, 458, 530, 605, 677, 713
BmgT120I GGNCC 7 cut(s) 134, 208, 458, 530, 605, 677, 713
BmiI GGNNCC 5 cut(s) 135, 136, 209, 271, 856
BmrFI CCNGG 1 cut(s) 918
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 4 cut(s) 789, 819, 880, 910
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 890
BseBI CCWGG 1 cut(s) 918
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 5 cut(s) 326, 599, 671, 767, 913
BseMI GCAATG 1 cut(s) 890
BseNI ACTGG 1 cut(s) 103
BseXI GCAGC 2 cut(s) 785, 895
BshFI GGCC 3 cut(s) 127, 210, 921
BsiHKAI GWGCWC 3 cut(s) 406, 574, 646
BsiSI CCGG 1 cut(s) 689
BslFI GGGAC 2 cut(s) 120, 212
BsmAI GTCTC 1 cut(s) 939
BsmFI GGGAC 2 cut(s) 120, 212
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 3 cut(s) 127, 210, 921
Bsp1286I GDGCHC 3 cut(s) 406, 574, 646
Bsp143I GATC 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 128
BspACI CCGC 3 cut(s) 543, 576, 623
BspANI GGCC 3 cut(s) 127, 210, 921
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 5 cut(s) 135, 136, 209, 271, 856
BspTI CTTAAG 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 890
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 1 cut(s) 212
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 128
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 2 cut(s) 148, 203
Bst6I CTCTTC 1 cut(s) 415
BstAFI CTTAAG 1 cut(s) 226
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 1 cut(s) 128
BstENI CCTNNNNNAGG 4 cut(s) 510, 726, 729, 846
BstF5I GGATG 5 cut(s) 326, 599, 671, 767, 913
BstKTI GATC 1 cut(s) 215
BstMAI GTCTC 1 cut(s) 939
BstMBI GATC 1 cut(s) 212
BstMWI GCNNNNNNNGC 3 cut(s) 35, 278, 584
BstNI CCWGG 1 cut(s) 918
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 916
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 2 cut(s) 785, 895
BstXI CCANNNNNNTGG 2 cut(s) 195, 924
BsuRI GGCC 3 cut(s) 127, 210, 921
BtgI CCRYGG 1 cut(s) 128
BtsCI GGATG 5 cut(s) 326, 599, 671, 767, 913
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 1 cut(s) 333
Cfr13I GGNCC 7 cut(s) 134, 208, 458, 530, 605, 677, 713
Csp6I GTAC 1 cut(s) 250
CviAII CATG 7 cut(s) 129, 334, 527, 602, 710, 770, 833
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 214
DpnII GATC 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Ecl136II GAGCTC 3 cut(s) 404, 572, 644
Eco130I CCWWGG 1 cut(s) 128
Eco147I AGGCCT 1 cut(s) 921
Eco24I GRGCYC 3 cut(s) 406, 574, 646
Eco32I GATATC 1 cut(s) 497
Eco47I GGWCC 6 cut(s) 134, 458, 530, 605, 677, 713
Eco53kI GAGCTC 3 cut(s) 404, 572, 644
EcoICRI GAGCTC 3 cut(s) 404, 572, 644
EcoNI CCTNNNNNAGG 4 cut(s) 510, 726, 729, 846
EcoO109I RGGNCCY 3 cut(s) 134, 458, 677
EcoRII CCWGG 1 cut(s) 916
EcoRV GATATC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 406, 574, 646
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 7 cut(s) 132, 337, 530, 605, 713, 773, 836
FaqI GGGAC 2 cut(s) 120, 212
FatI CATG 7 cut(s) 128, 333, 526, 601, 709, 769, 832
FauI CCCGC 3 cut(s) 550, 583, 616
Fnu4HI GCNGC 2 cut(s) 774, 909
FokI GGATG 5 cut(s) 313, 606, 678, 774, 900
FriOI GRGCYC 3 cut(s) 406, 574, 646
Fsp4HI GCNGC 2 cut(s) 774, 909
FspBI CTAG 1 cut(s) 549
GluI GCNGC 2 cut(s) 774, 909
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 3 cut(s) 127, 210, 921
HapII CCGG 1 cut(s) 689
Hin1II CATG 7 cut(s) 132, 337, 530, 605, 713, 773, 836
HinfI GANTC 2 cut(s) 50, 181
HpaII CCGG 1 cut(s) 689
HphI GGTGA 2 cut(s) 45, 257
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 3 cut(s) 16, 379, 419
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 6 cut(s) 102, 117, 340, 425, 548, 624
HpyCH4III ACNGT 2 cut(s) 148, 203
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 7 cut(s) 4, 68, 587, 659, 773, 818, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 278, 584
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 7 cut(s) 132, 337, 530, 605, 713, 773, 836
KflI GGGWCCC 1 cut(s) 134
Kzo9I GATC 1 cut(s) 212
Lsp1109I GCAGC 2 cut(s) 785, 895
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 549
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 274, 307
MalI GATC 1 cut(s) 214
MbiI CCGCTC 1 cut(s) 578
MboI GATC 1 cut(s) 212
MboII GAAGA 1 cut(s) 432
MhlI GDGCHC 3 cut(s) 406, 574, 646
MluCI AATT 2 cut(s) 320, 357
MmeI TCCRAC 2 cut(s) 371, 397
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 227, 953
MslI CAYNNNNRTG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 226
MspI CCGG 1 cut(s) 689
MspR9I CCNGG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 918
MwoI GCNNNNNNNGC 3 cut(s) 35, 278, 584
NcoI CCATGG 1 cut(s) 128
NdeII GATC 1 cut(s) 212
NlaIII CATG 7 cut(s) 132, 337, 530, 605, 713, 773, 836
NlaIV GGNNCC 5 cut(s) 135, 136, 209, 271, 856
NmuCI GTSAC 1 cut(s) 274
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 773
OliI CACNNNNGTG 1 cut(s) 101
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 921
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 2 cut(s) 50, 181
PkrI GCNGC 2 cut(s) 775, 910
PpuMI RGGWCCY 3 cut(s) 134, 458, 677
Psp124BI GAGCTC 3 cut(s) 406, 574, 646
Psp1406I AACGTT 1 cut(s) 306
Psp5II RGGWCCY 3 cut(s) 134, 458, 677
Psp6I CCWGG 1 cut(s) 916
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 5 cut(s) 135, 136, 209, 271, 856
PspPI GGNCC 7 cut(s) 134, 208, 458, 530, 605, 677, 713
PspPPI RGGWCCY 3 cut(s) 134, 458, 677
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 101
SacI GAGCTC 3 cut(s) 406, 574, 646
SaqAI TTAA 2 cut(s) 227, 953
SatI GCNGC 2 cut(s) 774, 909
Sau3AI GATC 1 cut(s) 212
Sau96I GGNCC 7 cut(s) 134, 208, 458, 530, 605, 677, 713
ScrFI CCNGG 1 cut(s) 918
SduI GDGCHC 3 cut(s) 406, 574, 646
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 6 cut(s) 134, 458, 530, 605, 677, 713
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 2 cut(s) 320, 357
SseBI AGGCCT 1 cut(s) 921
SsiI CCGC 3 cut(s) 543, 576, 623
SspMI CTAG 1 cut(s) 549
SstI GAGCTC 3 cut(s) 406, 574, 646
StuI AGGCCT 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 916
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 2 cut(s) 148, 203
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 143, 864
TasI AATT 2 cut(s) 320, 357
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 2 cut(s) 50, 181
Tru1I TTAA 2 cut(s) 227, 953
Tru9I TTAA 2 cut(s) 227, 953
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 773, 908
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 322, 350
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 226
VpaK11BI GGWCC 6 cut(s) 134, 458, 530, 605, 677, 713
XagI CCTNNNNNAGG 4 cut(s) 510, 726, 729, 846
XapI RAATTY 1 cut(s) 320
XceI RCATGY 1 cut(s) 773
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.