FvH4_3g36820

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003654
Physical Location & Seq
Reverse (-)
31534462 .. 31536028
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36820.t2

Sequence Viewer

Length: 1014 bp
ATGGGTGGTACTTTTATGATCAACTTGGTTTTCATTGCTTTCTATGCTTTCATGGCTATTTCGGATTCTCAAGCCGAAGGAACAAACACGAGTGCTTCAGCGATTCTCATAACAGTTGATCAATCCGGCAAAGGTGACTACAAGAAAATACAAGAAGCCATTGATTCAGTGGCGTCCAACAACGCAGAGCTCGTGTTCATTAGGGTTAAGCCCGGAGTTTATGAAGAAAAAGTTACAGTTCCGGCGGACAAGCCGTTTATAACAATAAGTGGCACAAAGGCAGCTGACACGGTTATAACATGGAACGATAGCGGGGAGATTTTCGAGTCTCCAACGTTCTCTGTGTTGGCTTCTGATTTTGTAGGGCGTTACCTCACAATTCAGAATACATATGGATCAGGTGGAAAAGCTGTGGCATTAAGGGTGTCCGGGGATAGAGCAGCATTCTATGGCTGCAGAATTTTGTCTCGTCAAGACACCCTACTTGATGATACTGGGAGGCACTACTACAACAACTGTTACATTGAAGGAGATGTCGACTTCATATGTGGAAATGCTGCTTCTTTCTACGAGAAGTGCCATTTGCATTCTCTTTCGCTAGGAACCGGTGCCATAACGGCTCAACGAAGAATGTCTCCGGCTGAGGACACGGGGTTCACGTTCTTCGGGTGCAAGATCACCGGCGTCAAGACGGCTGTGTTGGGAAGGCCTTGGGGTACATATTCAAGAGTGGTGTTTGCCTTCAGTTACATGACTAGCGCCGTGCTACCACAAGGTTGGGACGATTGGGGAAACTCAGCCAACCAATTAAGCACCGTATACTATGGGCAGTATAAATGTTCTGGACGAGGAGCAACGACATCGAAAAGAGTAGAATGGGCACGAGACCTTACAAGCGAAGAAGTCGAACCGTTCTTGACAAAGGACTCGATTGATGCCAATGCTTGGTTAAGGTCAGCACCAACCAGTTTCAAGAAGAAGGCACTTATTAGTTCCAATGATACCAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

338

Amino Acids

36.81

Weight (kDa)

6.0

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 37 - 311 3.9e-68 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 260, 296
AccB1I GGYRCC 1 cut(s) 608
AccB7I CCANNNNNTGG 1 cut(s) 945
AccI GTMKAC 2 cut(s) 537, 819
AciI CCGC 2 cut(s) 245, 312
AclI AACGTT 1 cut(s) 335
AclWI GGATC 1 cut(s) 403
AcsI RAATTY 1 cut(s) 459
AcuI CTGAAG 2 cut(s) 81, 727
AcyI GRCGYC 2 cut(s) 173, 684
AfaI GTAC 2 cut(s) 10, 718
AfiI CCNNNNNNNGG 1 cut(s) 945
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 3 cut(s) 527, 726, 973
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 3 cut(s) 190, 284, 410
AluI AGCT 3 cut(s) 190, 284, 410
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 4 cut(s) 333, 471, 639, 879
AlwI GGATC 1 cut(s) 403
AoxI GGCC 1 cut(s) 707
ApeKI GCWGC 4 cut(s) 281, 440, 453, 557
ApoI RAATTY 1 cut(s) 459
AsiGI ACCGGT 1 cut(s) 605
AspLEI GCGC 1 cut(s) 761
AsuC2I CCSGG 2 cut(s) 213, 430
AsuHPI GGTGA 2 cut(s) 146, 670
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 3 cut(s) 88, 191, 882
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 4 cut(s) 293, 440, 452, 544
BceAI ACGGC 4 cut(s) 238, 633, 708, 746
BcgI CGANNNNNNTGC 2 cut(s) 843, 877
BclI TGATCA 2 cut(s) 18, 118
BcnI CCSGG 2 cut(s) 213, 430
BcoDI GTCTC 4 cut(s) 333, 471, 639, 879
BfaI CTAG 2 cut(s) 599, 756
BfmI CTRYAG 1 cut(s) 454
BfoI RGCGCY 1 cut(s) 762
BglI GCCNNNNNGGC 1 cut(s) 617
BisI GCNGC 4 cut(s) 282, 441, 454, 558
BlsI GCNGC 4 cut(s) 283, 442, 455, 559
Bme1390I CCNGG 2 cut(s) 213, 430
BmiI GGNNCC 2 cut(s) 604, 610
BmrFI CCNGG 2 cut(s) 213, 430
BmrI ACTGGG 1 cut(s) 504
BmsI GCATC 1 cut(s) 925
BmuI ACTGGG 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 54
BpuMI CCSGG 2 cut(s) 213, 430
BsaHI GRCGYC 2 cut(s) 173, 684
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 429, 710
BsaWI WCCGGW 1 cut(s) 605
Bsc4I CCNNNNNNNGG 1 cut(s) 945
Bse118I RCCGGY 2 cut(s) 605, 680
Bse1I ACTGG 2 cut(s) 499, 966
Bse3DI GCAATG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 429, 710
BseLI CCNNNNNNNGG 1 cut(s) 945
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 2 cut(s) 633, 810
BseNI ACTGG 2 cut(s) 499, 966
BseRI GAGGAG 1 cut(s) 864
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 4 cut(s) 293, 440, 452, 544
BshFI GGCC 1 cut(s) 709
BshNI GGYRCC 1 cut(s) 608
BshTI ACCGGT 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 7 cut(s) 126, 213, 242, 429, 606, 638, 681
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 1 cut(s) 945
BsmAI GTCTC 4 cut(s) 333, 471, 639, 879
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 2 cut(s) 443, 586
BsnI GGCC 1 cut(s) 709
Bso31I GGTCTC 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 192, 883
Bsp143I GATC 4 cut(s) 18, 118, 395, 675
BspACI CCGC 2 cut(s) 245, 312
BspANI GGCC 1 cut(s) 709
BspCNI CTCAG 2 cut(s) 634, 809
BspLI GGNNCC 2 cut(s) 604, 610
BspMAI CTGCAG 1 cut(s) 458
BspPI GGATC 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 608
BspTNI GGTCTC 1 cut(s) 879
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 2 cut(s) 605, 680
BsrI ACTGG 2 cut(s) 499, 966
BssAI RCCGGY 2 cut(s) 605, 680
BssECI CCNNGG 2 cut(s) 429, 710
BssMI GATC 4 cut(s) 18, 118, 395, 675
BssNAI GTATAC 1 cut(s) 820
BssNI GRCGYC 2 cut(s) 173, 684
BssSI CACGAG 3 cut(s) 88, 191, 882
BssT1I CCWWGG 1 cut(s) 710
Bst1107I GTATAC 1 cut(s) 820
Bst2BI CACGAG 3 cut(s) 88, 191, 882
Bst4CI ACNGT 6 cut(s) 115, 238, 292, 518, 817, 912
BstACI GRCGYC 2 cut(s) 173, 684
BstDEI CTNAG 2 cut(s) 642, 796
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 1 cut(s) 761
BstKTI GATC 4 cut(s) 21, 121, 398, 678
BstMAI GTCTC 4 cut(s) 333, 471, 639, 879
BstMBI GATC 4 cut(s) 18, 118, 395, 675
BstMWI GCNNNNNNNGC 3 cut(s) 44, 53, 617
BstSCI CCNGG 2 cut(s) 211, 428
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 883
BstV1I GCAGC 4 cut(s) 293, 440, 452, 544
BstXI CCANNNNNNTGG 1 cut(s) 777
BstZ17I GTATAC 1 cut(s) 820
BsuRI GGCC 1 cut(s) 709
BtsIMutI CAGTG 1 cut(s) 174
CfoI GCGC 1 cut(s) 761
Cfr10I RCCGGY 2 cut(s) 605, 680
CseI GACGC 2 cut(s) 162, 673
Csp6I GTAC 2 cut(s) 9, 717
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 3 cut(s) 52, 300, 751
CviQI GTAC 2 cut(s) 9, 717
DdeI CTNAG 2 cut(s) 642, 796
DpnI GATC 4 cut(s) 20, 120, 397, 677
DpnII GATC 4 cut(s) 18, 118, 395, 675
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 710
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 879
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 81, 727
EcoICRI GAGCTC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 710
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 3 cut(s) 55, 303, 754
FaqI GGGAC 1 cut(s) 794
FatI CATG 3 cut(s) 51, 299, 750
FauI CCCGC 1 cut(s) 305
FauNDI CATATG 2 cut(s) 391, 545
FbaI TGATCA 2 cut(s) 18, 118
FblI GTMKAC 2 cut(s) 537, 819
Fnu4HI GCNGC 4 cut(s) 282, 441, 454, 558
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 282, 441, 454, 558
FspBI CTAG 2 cut(s) 599, 756
GlaI GCGC 1 cut(s) 760
GluI GCNGC 4 cut(s) 282, 441, 454, 558
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 1 cut(s) 709
HapII CCGG 7 cut(s) 126, 213, 242, 429, 606, 638, 681
HgaI GACGC 2 cut(s) 162, 673
HhaI GCGC 1 cut(s) 761
Hin1I GRCGYC 2 cut(s) 173, 684
Hin1II CATG 3 cut(s) 55, 303, 754
Hin6I GCGC 1 cut(s) 759
HinP1I GCGC 1 cut(s) 759
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 5 cut(s) 65, 103, 164, 326, 926
HpaII CCGG 7 cut(s) 126, 213, 242, 429, 606, 638, 681
HphI GGTGA 2 cut(s) 146, 670
Hpy166II GTNNAC 3 cut(s) 538, 657, 820
Hpy188I TCNGA 3 cut(s) 64, 355, 384
Hpy188III TCNNGA 6 cut(s) 473, 688, 726, 843, 916, 973
Hpy8I GTNNAC 3 cut(s) 538, 657, 820
HpyAV CCTTC 5 cut(s) 71, 521, 699, 751, 973
HpyCH4III ACNGT 6 cut(s) 115, 238, 292, 518, 817, 912
HpyCH4IV ACGT 2 cut(s) 335, 659
HpyCH4V TGCA 3 cut(s) 456, 586, 672
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 53, 617
HpyF3I CTNAG 2 cut(s) 642, 796
HpySE526I ACGT 2 cut(s) 335, 659
Hsp92I GRCGYC 2 cut(s) 173, 684
Hsp92II CATG 3 cut(s) 55, 303, 754
HspAI GCGC 1 cut(s) 759
Ksp22I TGATCA 2 cut(s) 18, 118
Kzo9I GATC 4 cut(s) 18, 118, 395, 675
LmnI GCTCC 1 cut(s) 851
Lsp1109I GCAGC 4 cut(s) 293, 440, 452, 544
LweI GCATC 1 cut(s) 925
MaeI CTAG 2 cut(s) 599, 756
MaeII ACGT 2 cut(s) 335, 659
MaeIII GTNAC 5 cut(s) 134, 232, 368, 518, 746
MalI GATC 4 cut(s) 20, 120, 397, 677
MboI GATC 4 cut(s) 18, 118, 395, 675
MboII GAAGA 5 cut(s) 236, 639, 655, 911, 988
MhlI GDGCHC 2 cut(s) 192, 883
MluCI AATT 4 cut(s) 378, 459, 806, 1009
MlyI GAGTC 2 cut(s) 335, 920
MmeI TCCRAC 2 cut(s) 201, 356
MnlI CCTC 4 cut(s) 383, 492, 637, 842
MseI TTAA 5 cut(s) 207, 419, 809, 950, 1012
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 7 cut(s) 126, 213, 242, 429, 606, 638, 681
MspR9I CCNGG 2 cut(s) 213, 430
Mva1269I GAATGC 2 cut(s) 443, 586
MwoI GCNNNNNNNGC 3 cut(s) 44, 53, 617
NciI CCSGG 2 cut(s) 213, 430
NdeI CATATG 2 cut(s) 391, 545
NdeII GATC 4 cut(s) 18, 118, 395, 675
NlaIII CATG 3 cut(s) 55, 303, 754
NlaIV GGNNCC 2 cut(s) 604, 610
NmuCI GTSAC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 709
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 656
PctI GAATGC 2 cut(s) 443, 586
PfeI GAWTC 3 cut(s) 65, 103, 164
PflMI CCANNNNNTGG 1 cut(s) 945
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 4 cut(s) 283, 442, 455, 559
PleI GAGTC 2 cut(s) 334, 920
PpsI GAGTC 2 cut(s) 334, 920
PsiI TTATAA 2 cut(s) 260, 296
Psp124BI GAGCTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 604, 610
PstI CTGCAG 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 10, 718
RsaNI GTAC 2 cut(s) 9, 717
SacI GAGCTC 1 cut(s) 192
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 5 cut(s) 207, 419, 809, 950, 1012
SatI GCNGC 4 cut(s) 282, 441, 454, 558
Sau3AI GATC 4 cut(s) 18, 118, 395, 675
SchI GAGTC 2 cut(s) 335, 920
ScrFI CCNGG 2 cut(s) 213, 430
SduI GDGCHC 2 cut(s) 192, 883
SfaNI GCATC 1 cut(s) 925
SfcI CTRYAG 1 cut(s) 454
SgrAI CRCCGGYG 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 4 cut(s) 378, 459, 806, 1009
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 2 cut(s) 245, 312
SspMI CTAG 2 cut(s) 599, 756
SstI GAGCTC 1 cut(s) 192
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 211, 428
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 6 cut(s) 115, 238, 292, 518, 817, 912
TaiI ACGT 2 cut(s) 338, 662
TaqI TCGA 5 cut(s) 324, 537, 863, 906, 929
TasI AATT 4 cut(s) 378, 459, 806, 1009
TfiI GAWTC 3 cut(s) 65, 103, 164
Tru1I TTAA 5 cut(s) 207, 419, 809, 950, 1012
Tru9I TTAA 5 cut(s) 207, 419, 809, 950, 1012
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 4 cut(s) 281, 440, 453, 557
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 5 cut(s) 22, 40, 187, 237, 532
TspRI CASTG 1 cut(s) 174
Van91I CCANNNNNTGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 459
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 537, 819
XspI CTAG 2 cut(s) 599, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.