RLG00000011285

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003654
Physical Location & Seq
Forward (+)
8242734 .. 8245704
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011285

Sequence Viewer

Length: 1212 bp
ATGGCGCGTAGGGCTCTAGTTAGAATGATTGTCATAATTTTGTATGCTTTCGTCACTATTTCTCAAGCGGAAAATATAATAACAGTTGATCAATCCGGTAACGGTCATTACCGTAAAATACAAGATGCTATTGATTCTGTTCCGTCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGAACTTACAAAGAAAGTATTACCGTACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAGAAGCAAGTGATGTGTTGATAACGTGGGATCAGAGAGGGGACATTGATAAAGTTGCAACATTTCATGTTTCGGGTTCCGACTTCGTAGCGCGCTACATAACAATTCAAAATACATTCGGGCCTGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAATAGCGTTTTATGACTGCTCAATATTAGGTTATCAGGATACTCTATTTGACGAAAAAGGCAGACACTACTACGAAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCGCAACGAAGACAAGCGTCAACAGATAGTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAAAGGCGAAAAAACATTTTTAGGAAGACCATGGGAGGGATCCTATGCTAGGGTCATATTTGCCTACACTTACATGGAAGATGTGATAGAACCACTAGGTTGGTCTCGTTATTCTGATGGATCCACAGAACAAGAATTAATGACAAACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAAAATTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAAGATTGGATAAAGTCTATACCGGTAACAACGGCACCACCAGTGATTCCGTTGTTGGCCCCACCAGCACCGGTGGTGATCTTGCCACCAGTGACACCATCACACATGGACGGCAGAGCGCAAGAGGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGCATCCTCTTCAATCTTTAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTAACCTCGGCAATTTGCTTAACTGAGGTTGTGGATGGAATTGGATCAATTAGTCGGTCTCCTATGCCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

404

Amino Acids

44.31

Weight (kDa)

6.0

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 308 2.5e-67 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 958
AccII CGCG 3 cut(s) 7, 343, 868
AciI CCGC 2 cut(s) 68, 1119
AclWI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1189
AcsI RAATTY 2 cut(s) 872, 895
AfaI GTAC 2 cut(s) 210, 244
AfiI CCNNNNNNNGG 3 cut(s) 575, 689, 702
AgeI ACCGGT 2 cut(s) 946, 994
AgsI TTSAA 5 cut(s) 359, 503, 548, 820, 1098
AjnI CCWGG 2 cut(s) 181, 373
Alw26I GTCTC 3 cut(s) 406, 762, 1200
AlwI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1189
Ama87I CYCGRG 1 cut(s) 1081
AoxI GGCC 2 cut(s) 371, 981
ApoI RAATTY 2 cut(s) 872, 895
AseI ATTAAT 1 cut(s) 791
AsiGI ACCGGT 2 cut(s) 946, 994
Asp700I GAANNNNTTC 1 cut(s) 1112
AspLEI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1045
AspS9I GGNCC 2 cut(s) 371, 982
AsuHPI GGTGA 2 cut(s) 1012, 1064
AvaI CYCGRG 1 cut(s) 1081
BamHI GGATCC 3 cut(s) 157, 692, 773
BanI GGYRCC 1 cut(s) 958
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 604, 685
BccI CCATC 3 cut(s) 764, 1030, 1166
BceAI ACGGC 2 cut(s) 972, 1051
BciT130I CCWGG 2 cut(s) 183, 375
BciVI GTATCC 1 cut(s) 445
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 3 cut(s) 406, 762, 1200
BfaI CTAG 4 cut(s) 17, 702, 749, 833
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 1 cut(s) 1120
BlsI GCNGC 1 cut(s) 1121
Bme1390I CCNGG 2 cut(s) 183, 375
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 2 cut(s) 371, 982
BmiI GGNNCC 6 cut(s) 159, 328, 694, 775, 960, 984
BmrFI CCNGG 2 cut(s) 183, 375
BmsI GCATC 2 cut(s) 115, 1097
BoxI GACNNNNGTC 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 604, 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 48
BsaI GGTCTC 3 cut(s) 406, 762, 1200
BsaJI CCNNGG 3 cut(s) 683, 1080, 1143
BsaWI WCCGGW 3 cut(s) 95, 946, 994
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 612, 642
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 689, 702
Bse118I RCCGGY 2 cut(s) 946, 994
Bse1I ACTGG 5 cut(s) 534, 655, 965, 1013, 1205
BseBI CCWGG 2 cut(s) 183, 375
BseDI CCNNGG 3 cut(s) 683, 1080, 1143
BseGI GGATG 3 cut(s) 845, 1088, 1177
BseLI CCNNNNNNNGG 3 cut(s) 575, 689, 702
BseMII CTCAG 1 cut(s) 1152
BseNI ACTGG 5 cut(s) 534, 655, 965, 1013, 1205
BsePI GCGCGC 1 cut(s) 341
Bsh1236I CGCG 3 cut(s) 7, 343, 868
BshFI GGCC 2 cut(s) 373, 983
BshNI GGYRCC 1 cut(s) 958
BshTI ACCGGT 2 cut(s) 946, 994
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 4 cut(s) 96, 405, 947, 995
BslFI GGGAC 2 cut(s) 305, 1141
BslI CCNNNNNNNGG 3 cut(s) 575, 689, 702
BsmAI GTCTC 3 cut(s) 406, 762, 1200
BsmFI GGGAC 2 cut(s) 305, 1141
BsnI GGCC 2 cut(s) 373, 983
Bso31I GGTCTC 3 cut(s) 406, 762, 1200
BsoBI CYCGRG 1 cut(s) 1081
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 2 cut(s) 68, 1119
BspANI GGCC 2 cut(s) 373, 983
BspCNI CTCAG 1 cut(s) 1153
BspFNI CGCG 3 cut(s) 7, 343, 868
BspLI GGNNCC 6 cut(s) 159, 328, 694, 775, 960, 984
BspPI GGATC 8 cut(s) 152, 165, 288, 687, 700, 768, 781, 1189
BspT107I GGYRCC 1 cut(s) 958
BspTNI GGTCTC 3 cut(s) 406, 762, 1200
BsrFI RCCGGY 2 cut(s) 946, 994
BsrI ACTGG 5 cut(s) 534, 655, 965, 1013, 1205
BssAI RCCGGY 2 cut(s) 946, 994
BssECI CCNNGG 3 cut(s) 683, 1080, 1143
BssHII GCGCGC 1 cut(s) 341
BssMI GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
BssT1I CCWWGG 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 183, 375
Bst4CI ACNGT 4 cut(s) 85, 104, 113, 208
Bst6I CTCTTC 1 cut(s) 1099
BstC8I GCNNGC 2 cut(s) 343, 1134
BstDEI CTNAG 2 cut(s) 395, 1161
BstDSI CCRYGG 1 cut(s) 683
BstENI CCTNNNNNAGG 2 cut(s) 573, 700
BstF5I GGATG 3 cut(s) 845, 1088, 1177
BstFNI CGCG 3 cut(s) 7, 343, 868
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1045
BstKTI GATC 8 cut(s) 91, 160, 177, 283, 695, 776, 1005, 1184
BstMAI GTCTC 3 cut(s) 406, 762, 1200
BstMBI GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
BstMWI GCNNNNNNNGC 2 cut(s) 11, 989
BstNI CCWGG 2 cut(s) 183, 375
BstPAI GACNNNNGTC 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 181, 373
BstSFI CTRYAG 2 cut(s) 249, 570
BstUI CGCG 3 cut(s) 7, 343, 868
BstV2I GAAGAC 2 cut(s) 604, 685
BstX2I RGATCY 3 cut(s) 157, 692, 773
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 3 cut(s) 157, 692, 773
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 2 cut(s) 373, 983
BtgI CCRYGG 1 cut(s) 683
BtsCI GGATG 3 cut(s) 845, 1088, 1177
BtsIMutI CAGTG 3 cut(s) 883, 972, 1020
Cac8I GCNNGC 2 cut(s) 343, 1134
CfoI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1045
Cfr10I RCCGGY 2 cut(s) 946, 994
Cfr13I GGNCC 2 cut(s) 371, 982
CseI GACGC 2 cut(s) 594, 1138
Csp6I GTAC 2 cut(s) 209, 243
CspAI ACCGGT 2 cut(s) 946, 994
CviAII CATG 5 cut(s) 317, 684, 727, 1030, 1066
CviJI RGCY 6 cut(s) 14, 181, 223, 373, 983, 1136
CviKI_1 RGCY 6 cut(s) 14, 181, 223, 373, 983, 1136
CviQI GTAC 2 cut(s) 209, 243
DdeI CTNAG 2 cut(s) 395, 1161
DpnI GATC 8 cut(s) 90, 159, 176, 282, 694, 775, 1004, 1183
DpnII GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
Eam1104I CTCTTC 1 cut(s) 1099
EarI CTCTTC 1 cut(s) 1099
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 3 cut(s) 406, 762, 1200
Eco88I CYCGRG 1 cut(s) 1081
EcoNI CCTNNNNNAGG 2 cut(s) 573, 700
EcoRII CCWGG 2 cut(s) 181, 373
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 5 cut(s) 320, 687, 730, 1033, 1069
FaqI GGGAC 2 cut(s) 305, 1141
FatI CATG 5 cut(s) 316, 683, 726, 1029, 1065
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 1120
FokI GGATG 3 cut(s) 832, 1075, 1184
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 1 cut(s) 1120
FspBI CTAG 4 cut(s) 17, 702, 749, 833
GlaI GCGC 8 cut(s) 6, 342, 344, 393, 532, 591, 867, 1044
GluI GCNGC 1 cut(s) 1120
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 2 cut(s) 373, 983
HapII CCGG 4 cut(s) 96, 405, 947, 995
HgaI GACGC 2 cut(s) 594, 1138
HhaI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1045
Hin1II CATG 5 cut(s) 320, 687, 730, 1033, 1069
Hin6I GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1043
HinP1I GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1043
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 2 cut(s) 134, 970
HpaII CCGG 4 cut(s) 96, 405, 947, 995
HphI GGTGA 2 cut(s) 1012, 1064
Hpy166II GTNNAC 3 cut(s) 609, 812, 1055
Hpy188I TCNGA 4 cut(s) 285, 331, 769, 1211
Hpy188III TCNNGA 2 cut(s) 215, 449
Hpy8I GTNNAC 3 cut(s) 609, 812, 1055
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 4 cut(s) 85, 104, 113, 208
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 2 cut(s) 308, 893
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 989
HpyF3I CTNAG 2 cut(s) 395, 1161
HpySE526I ACGT 1 cut(s) 275
Hsp92II CATG 5 cut(s) 320, 687, 730, 1033, 1069
HspAI GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1043
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
LmnI GCTCC 1 cut(s) 836
LweI GCATC 2 cut(s) 115, 1097
MaeI CTAG 4 cut(s) 17, 702, 749, 833
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 6 cut(s) 52, 98, 494, 554, 949, 1015
MalI GATC 8 cut(s) 90, 159, 176, 282, 694, 775, 1004, 1183
MboI GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
MboII GAAGA 4 cut(s) 609, 690, 743, 1086
MflI RGATCY 3 cut(s) 157, 692, 773
MhlI GDGCHC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 354, 873
MroXI GAANNNNTTC 1 cut(s) 1112
MseI TTAA 6 cut(s) 236, 636, 791, 858, 1104, 1157
MslI CAYNNNNRTG 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 1119
MspI CCGG 4 cut(s) 96, 405, 947, 995
MspR9I CCNGG 2 cut(s) 183, 375
MvaI CCWGG 2 cut(s) 183, 375
MvnI CGCG 3 cut(s) 7, 343, 868
MwoI GCNNNNNNNGC 2 cut(s) 11, 989
NcoI CCATGG 1 cut(s) 683
NdeII GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
NlaIII CATG 5 cut(s) 320, 687, 730, 1033, 1069
NlaIV GGNNCC 6 cut(s) 159, 328, 694, 775, 960, 984
NmeAIII GCCGAG 1 cut(s) 1124
NmuCI GTSAC 3 cut(s) 52, 554, 1015
PauI GCGCGC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 1112
PfeI GAWTC 2 cut(s) 134, 970
PinAI ACCGGT 2 cut(s) 946, 994
PkrI GCNGC 1 cut(s) 1121
PshAI GACNNNNGTC 1 cut(s) 604
PshBI ATTAAT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 181, 373
PspGI CCWGG 2 cut(s) 181, 373
PspN4I GGNNCC 6 cut(s) 159, 328, 694, 775, 960, 984
PspPI GGNCC 2 cut(s) 371, 982
PsuI RGATCY 3 cut(s) 157, 692, 773
PteI GCGCGC 1 cut(s) 341
RsaI GTAC 2 cut(s) 210, 244
RsaNI GTAC 2 cut(s) 209, 243
RseI CAYNNNNRTG 1 cut(s) 725
SaqAI TTAA 6 cut(s) 236, 636, 791, 858, 1104, 1157
SatI GCNGC 1 cut(s) 1120
Sau3AI GATC 8 cut(s) 88, 157, 174, 280, 692, 773, 1002, 1181
Sau96I GGNCC 2 cut(s) 371, 982
ScrFI CCNGG 2 cut(s) 183, 375
SduI GDGCHC 1 cut(s) 16
SetI ASST 7 cut(s) 214, 278, 445, 754, 1060, 1145, 1167
SfaNI GCATC 2 cut(s) 115, 1097
SfcI CTRYAG 2 cut(s) 249, 570
SgrAI CRCCGGYG 1 cut(s) 994
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
SsiI CCGC 2 cut(s) 68, 1119
SspI AATATT 1 cut(s) 438
SspMI CTAG 4 cut(s) 17, 702, 749, 833
StyD4I CCNGG 2 cut(s) 181, 373
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 4 cut(s) 85, 104, 113, 208
TaiI ACGT 1 cut(s) 278
TaqI TCGA 1 cut(s) 173
TaqII GACCGA 1 cut(s) 1182
TauI GCSGC 1 cut(s) 1122
TfiI GAWTC 2 cut(s) 134, 970
Tru1I TTAA 6 cut(s) 236, 636, 791, 858, 1104, 1157
Tru9I TTAA 6 cut(s) 236, 636, 791, 858, 1104, 1157
TscAI CASTG 3 cut(s) 883, 972, 1020
TseFI GTSAC 3 cut(s) 52, 554, 1015
Tsp45I GTSAC 3 cut(s) 52, 554, 1015
TspDTI ATGAA 2 cut(s) 217, 305
TspGWI ACGGA 3 cut(s) 132, 843, 963
TspRI CASTG 3 cut(s) 883, 972, 1020
VspI ATTAAT 1 cut(s) 791
XagI CCTNNNNNAGG 2 cut(s) 573, 700
XapI RAATTY 2 cut(s) 872, 895
XmnI GAANNNNTTC 1 cut(s) 1112
XspI CTAG 4 cut(s) 17, 702, 749, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.