MD15G1014700.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003654
Physical Location & Seq
Forward (+)
862632 .. 864047
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1014700.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGCTTGTGGTGCTGCTAATTTCACGTGTTTATTCGTCATGGCAATACTAATGCAAGCTTTCATGGTCGTGCTCTCTCAACCAGTCGAAGCAGAAGCAATCTCGAGGTCCTCGAAAAGATTCATCACCGTCGACAAATCCGGCAACGGCGATTACACCAAAATACAGTACGCAATCGACGCCGTGCCGTCAGGTAATGCTCAGCTTGTGTTTATTTGGGTCAAGCCTGGTGTGTACCATGAAAAAATAGTTGTACCTTCGGACAAACCCTATATAACAGTAAGTGGCACGGGTACTGCACATGAGACTGTAATAATATGGAACGAATGCGGAGAGATTTTTGATGTTGCCGTCTTTACCGTGTTGGCTACCGATTTTATAGGCCGCTTTATCACCATTCAGAATACATATGGAATTGGTGCGAAAGCAGTGGCGCTTAGAGTCTCGGCCGATAGAGCAGCATTCTACGCATGTAGAATTTTGTCTCGGCAAGATGCCTTGTTCGACCACGTCGGCAGGCACTACTACGGACACTGCTACATTGAAGGCGACACCGATTTAATTTTCGGAAACGCTGCTTCTTTCTTCGAGAAGTGTCATTTGCATTCGCTTTCGGAGCACAAAGGAGCGATCACAGCACAACGAAGGTATGCGCCTTCCGATCAATCGGGGTTCACCTTCTTGGCATGCGTCGTCACCGGCGTCAAAACGGCACTACTAGGACGGCCGTGGGGTCCCTATTCGACAGTCGTCTTTGCGTATACTTACATGTCAAACGTGATTCTTCCAGAAGGGTGGGACGGATGGAGACTAGCACCCAACAACTTAAGTCATGTTTACTATGGGCAATACAAATGCTATGGACCGGGAGCCGAGACATCACAAAGAGTTAAATGGTCACAAAGCCTTACAAGCGAACAAGTTACACCCTTCCTTGATCAAGCGATTGCCCTTCAATCCGAAAGCTGGCAAGATCGTTGGTCTCTCCTTCATCCTTACGGAGCAGAGCAAGGAAGCGTGGACGGTAAGAACAATATGGACCAAATAGGAGAGGATCCCAGCCCCATCGGAGAGGACGTGGGGGTTGGTTGGGACCCTGTGGAATGGAGTGGGGCGGAGGGGATGTCGATCAGCCCAGCGGAGGATATGTCGAGCTGCGAGAGTCCTAGGTCTCCCGGGATTTTTCTTTTTCTTCTTTTTGGTTTATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

405

Amino Acids

44.43

Weight (kDa)

5.2

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 42 - 302 4.5e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 132, 761
AciI CCGC 4 cut(s) 330, 385, 1115, 1139
AclWI GGATC 2 cut(s) 1049, 1062
AcoI YGGCCR 2 cut(s) 447, 725
AcsI RAATTY 1 cut(s) 477
AcvI CACGTG 1 cut(s) 27
AcyI GRCGYC 2 cut(s) 180, 702
AfaI GTAC 4 cut(s) 170, 236, 255, 295
AfiI CCNNNNNNNGG 2 cut(s) 966, 1141
AflII CTTAAG 1 cut(s) 826
AflIII ACRYGT 2 cut(s) 26, 768
AgsI TTSAA 2 cut(s) 545, 956
AjiI CACGTC 2 cut(s) 511, 1078
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 4 cut(s) 59, 205, 966, 1155
AluI AGCT 4 cut(s) 59, 205, 966, 1155
Alw21I GWGCWC 2 cut(s) 75, 621
Alw26I GTCTC 7 cut(s) 299, 448, 489, 802, 869, 987, 1176
AlwI GGATC 2 cut(s) 1049, 1062
Ama87I CYCGRG 2 cut(s) 103, 1175
AoxI GGCC 3 cut(s) 382, 447, 725
ApeKI GCWGC 4 cut(s) 14, 458, 575, 1155
ApoI RAATTY 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 119
AspA2I CCTAGG 1 cut(s) 1166
AspLEI GCGC 2 cut(s) 436, 655
AspS9I GGNCC 5 cut(s) 108, 734, 863, 1039, 1093
AsuC2I CCSGG 3 cut(s) 867, 1176, 1177
AsuHPI GGTGA 4 cut(s) 118, 385, 667, 688
AvaI CYCGRG 2 cut(s) 103, 1175
AvaII GGWCC 5 cut(s) 108, 734, 863, 1039, 1093
AvrII CCTAGG 1 cut(s) 1166
BamHI GGATCC 1 cut(s) 1054
BbrPI CACGTG 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 75, 621
BbvI GCAGC 3 cut(s) 470, 562, 1142
BccI CCATC 2 cut(s) 798, 1073
BceAI ACGGC 7 cut(s) 163, 167, 172, 335, 712, 726, 740
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 937
BcnI CCSGG 3 cut(s) 867, 1176, 1177
BcoDI GTCTC 7 cut(s) 299, 448, 489, 802, 869, 987, 1176
BfaI CTAG 3 cut(s) 719, 812, 1167
BfoI RGCGCY 1 cut(s) 437
BfrI CTTAAG 1 cut(s) 826
BisI GCNGC 5 cut(s) 15, 385, 459, 576, 1156
BlnI CCTAGG 1 cut(s) 1166
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 5 cut(s) 16, 386, 460, 577, 1157
Bme1390I CCNGG 4 cut(s) 228, 867, 1176, 1177
Bme18I GGWCC 5 cut(s) 108, 734, 863, 1039, 1093
BmeT110I CYCGRG 2 cut(s) 103, 1175
BmgBI CACGTC 2 cut(s) 511, 1078
BmgT120I GGNCC 5 cut(s) 108, 734, 863, 1039, 1093
BmiI GGNNCC 6 cut(s) 735, 736, 871, 1056, 1094, 1095
BmrFI CCNGG 4 cut(s) 228, 867, 1176, 1177
BmsI GCATC 1 cut(s) 484
BoxI GACNNNNGTC 1 cut(s) 749
Bpu1102I GCTNAGC 1 cut(s) 201
BpuMI CCSGG 3 cut(s) 867, 1176, 1177
BsaAI YACGTR 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 1127
BsaHI GRCGYC 2 cut(s) 180, 702
BsaI GGTCTC 2 cut(s) 987, 1176
BsaJI CCNNGG 3 cut(s) 728, 1166, 1175
BsaXI ACNNNNNCTCC 6 cut(s) 1062, 1092, 1109, 1133, 1139, 1163
Bsc4I CCNNNNNNNGG 2 cut(s) 966, 1141
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 1127
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 728, 1166, 1175
BseGI GGATG 3 cut(s) 809, 991, 1128
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 2 cut(s) 966, 1141
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 83
BseX3I CGGCCG 2 cut(s) 447, 725
BseXI GCAGC 3 cut(s) 470, 562, 1142
BseYI CCCAGC 2 cut(s) 1058, 1135
BsgI GTGCAG 1 cut(s) 282
Bsh1285I CGRYCG 2 cut(s) 450, 728
BshFI GGCC 3 cut(s) 384, 449, 727
BsiEI CGRYCG 2 cut(s) 450, 728
BsiHKAI GWGCWC 2 cut(s) 75, 621
BsiHKCI CYCGRG 2 cut(s) 103, 1175
BsiSI CCGG 4 cut(s) 141, 699, 866, 1176
BslFI GGGAC 3 cut(s) 720, 812, 1106
BslI CCNNNNNNNGG 2 cut(s) 966, 1141
BsmAI GTCTC 7 cut(s) 299, 448, 489, 802, 869, 987, 1176
BsmFI GGGAC 3 cut(s) 720, 812, 1106
BsmI GAATGC 3 cut(s) 332, 461, 604
BsnI GGCC 3 cut(s) 384, 449, 727
Bso31I GGTCTC 2 cut(s) 987, 1176
BsoBI CYCGRG 2 cut(s) 103, 1175
Bsp1286I GDGCHC 2 cut(s) 75, 621
Bsp143I GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 4 cut(s) 330, 385, 1115, 1139
BspANI GGCC 3 cut(s) 384, 449, 727
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 6 cut(s) 735, 736, 871, 1056, 1094, 1095
BspPI GGATC 2 cut(s) 1049, 1062
BspTI CTTAAG 1 cut(s) 826
BspTNI GGTCTC 2 cut(s) 987, 1176
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 3 cut(s) 728, 1166, 1175
BssMI GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
BssNAI GTATAC 1 cut(s) 762
BssNI GRCGYC 2 cut(s) 180, 702
BssT1I CCWWGG 1 cut(s) 1166
Bst1107I GTATAC 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 7 cut(s) 130, 168, 280, 310, 361, 748, 1025
BstACI GRCGYC 2 cut(s) 180, 702
BstAFI CTTAAG 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 27
BstC8I GCNNGC 4 cut(s) 57, 518, 688, 968
BstDEI CTNAG 2 cut(s) 201, 437
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 3 cut(s) 809, 991, 1128
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 2 cut(s) 436, 655
BstKTI GATC 6 cut(s) 633, 664, 940, 976, 1057, 1131
BstMAI GTCTC 7 cut(s) 299, 448, 489, 802, 869, 987, 1176
BstMBI GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
BstMCI CGRYCG 2 cut(s) 450, 728
BstMWI GCNNNNNNNGC 7 cut(s) 11, 179, 455, 467, 616, 635, 912
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 3 cut(s) 474, 690, 772
BstPAI GACNNNNGTC 1 cut(s) 749
BstSCI CCNGG 4 cut(s) 226, 865, 1174, 1175
BstV1I GCAGC 3 cut(s) 470, 562, 1142
BstX2I RGATCY 1 cut(s) 1054
BstXI CCANNNNNNTGG 1 cut(s) 795
BstYI RGATCY 1 cut(s) 1054
BstZ17I GTATAC 1 cut(s) 762
BstZI CGGCCG 2 cut(s) 447, 725
BsuRI GGCC 3 cut(s) 384, 449, 727
BtgI CCRYGG 1 cut(s) 728
BtrI CACGTC 2 cut(s) 511, 1078
BtsCI GGATG 3 cut(s) 809, 991, 1128
BtsI GCAGTG 2 cut(s) 435, 532
BtsIMutI CAGTG 2 cut(s) 435, 532
Cac8I GCNNGC 4 cut(s) 57, 518, 688, 968
CfoI GCGC 2 cut(s) 436, 655
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 5 cut(s) 108, 734, 863, 1039, 1093
Cfr9I CCCGGG 1 cut(s) 1175
CseI GACGC 3 cut(s) 188, 679, 691
Csp6I GTAC 4 cut(s) 169, 235, 254, 294
CviAII CATG 8 cut(s) 40, 64, 239, 302, 471, 687, 769, 833
CviQI GTAC 4 cut(s) 169, 235, 254, 294
DdeI CTNAG 2 cut(s) 201, 437
DpnI GATC 6 cut(s) 632, 663, 939, 975, 1056, 1130
DpnII GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
EaeI YGGCCR 2 cut(s) 447, 725
EagI CGGCCG 2 cut(s) 447, 725
EciI GGCGGA 1 cut(s) 1130
EclXI CGGCCG 2 cut(s) 447, 725
Eco130I CCWWGG 1 cut(s) 1166
Eco31I GGTCTC 2 cut(s) 987, 1176
Eco47I GGWCC 5 cut(s) 108, 734, 863, 1039, 1093
Eco52I CGGCCG 2 cut(s) 447, 725
Eco72I CACGTG 1 cut(s) 27
Eco88I CYCGRG 2 cut(s) 103, 1175
EcoO109I RGGNCCY 3 cut(s) 108, 734, 1093
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 1166
ErhI CCWWGG 1 cut(s) 1166
FaeI CATG 8 cut(s) 43, 67, 242, 305, 474, 690, 772, 836
FaqI GGGAC 3 cut(s) 720, 812, 1106
FatI CATG 8 cut(s) 39, 63, 238, 301, 470, 686, 768, 832
FauNDI CATATG 1 cut(s) 409
FbaI TGATCA 1 cut(s) 937
FblI GTMKAC 2 cut(s) 132, 761
Fnu4HI GCNGC 5 cut(s) 15, 385, 459, 576, 1156
FokI GGATG 3 cut(s) 816, 978, 1135
Fsp4HI GCNGC 5 cut(s) 15, 385, 459, 576, 1156
FspBI CTAG 3 cut(s) 719, 812, 1167
GlaI GCGC 2 cut(s) 435, 654
GluI GCNGC 5 cut(s) 15, 385, 459, 576, 1156
GsaI CCCAGC 2 cut(s) 1062, 1139
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 3 cut(s) 384, 449, 727
HapII CCGG 4 cut(s) 141, 699, 866, 1176
HgaI GACGC 3 cut(s) 188, 679, 691
HhaI GCGC 2 cut(s) 436, 655
Hin1I GRCGYC 2 cut(s) 180, 702
Hin1II CATG 8 cut(s) 43, 67, 242, 305, 474, 690, 772, 836
Hin6I GCGC 2 cut(s) 434, 653
HinP1I GCGC 2 cut(s) 434, 653
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 4 cut(s) 120, 441, 781, 1162
HpaII CCGG 4 cut(s) 141, 699, 866, 1176
HphI GGTGA 4 cut(s) 118, 385, 667, 688
Hpy166II GTNNAC 6 cut(s) 133, 235, 675, 762, 838, 1021
Hpy188I TCNGA 7 cut(s) 262, 402, 569, 616, 661, 961, 1070
Hpy188III TCNNGA 3 cut(s) 103, 589, 788
Hpy8I GTNNAC 6 cut(s) 133, 235, 675, 762, 838, 1021
Hpy99I CGWCG 4 cut(s) 134, 182, 515, 695
HpyAV CCTTC 9 cut(s) 267, 539, 639, 666, 688, 785, 940, 962, 998
HpyCH4III ACNGT 7 cut(s) 130, 168, 280, 310, 361, 748, 1025
HpyCH4IV ACGT 4 cut(s) 26, 510, 777, 1077
HpyCH4V TGCA 3 cut(s) 55, 299, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 179, 455, 467, 616, 635, 912
HpyF3I CTNAG 2 cut(s) 201, 437
HpySE526I ACGT 4 cut(s) 26, 510, 777, 1077
Hsp92I GRCGYC 2 cut(s) 180, 702
Hsp92II CATG 8 cut(s) 43, 67, 242, 305, 474, 690, 772, 836
HspAI GCGC 2 cut(s) 434, 653
KflI GGGWCCC 2 cut(s) 734, 1093
Ksp22I TGATCA 1 cut(s) 937
Kzo9I GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
LmnI GCTCC 4 cut(s) 616, 626, 869, 1001
Lsp1109I GCAGC 3 cut(s) 470, 562, 1142
LweI GCATC 1 cut(s) 484
MaeI CTAG 3 cut(s) 719, 812, 1167
MaeII ACGT 4 cut(s) 26, 510, 777, 1077
MaeIII GTNAC 3 cut(s) 694, 897, 922
MalI GATC 6 cut(s) 632, 663, 939, 975, 1056, 1130
MboI GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
MboII GAAGA 3 cut(s) 577, 776, 1184
MflI RGATCY 1 cut(s) 1054
MhlI GDGCHC 2 cut(s) 75, 621
MluCI AATT 4 cut(s) 19, 414, 477, 561
MlyI GAGTC 2 cut(s) 450, 1171
MnlI CCTC 6 cut(s) 99, 121, 1045, 1066, 1111, 1135
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 3 cut(s) 560, 827, 891
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 1139
MspCI CTTAAG 1 cut(s) 826
MspI CCGG 4 cut(s) 141, 699, 866, 1176
MspR9I CCNGG 4 cut(s) 228, 867, 1176, 1177
Mva1269I GAATGC 3 cut(s) 332, 461, 604
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 7 cut(s) 11, 179, 455, 467, 616, 635, 912
NciI CCSGG 3 cut(s) 867, 1176, 1177
NdeI CATATG 1 cut(s) 409
NdeII GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
NlaIII CATG 8 cut(s) 43, 67, 242, 305, 474, 690, 772, 836
NlaIV GGNNCC 6 cut(s) 735, 736, 871, 1056, 1094, 1095
NmeAIII GCCGAG 3 cut(s) 425, 466, 898
NmuCI GTSAC 2 cut(s) 694, 897
NspI RCATGY 3 cut(s) 474, 690, 772
PaeI GCATGC 1 cut(s) 690
PaeR7I CTCGAG 1 cut(s) 103
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 3 cut(s) 110, 699, 1074
PctI GAATGC 3 cut(s) 332, 461, 604
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 120, 781
PflFI GACNNNGTC 1 cut(s) 509
PkrI GCNGC 5 cut(s) 16, 386, 460, 577, 1157
PleI GAGTC 2 cut(s) 449, 1170
PmaCI CACGTG 1 cut(s) 27
PmlI CACGTG 1 cut(s) 27
PpsI GAGTC 2 cut(s) 449, 1170
Ppu21I YACGTR 1 cut(s) 27
PpuMI RGGWCCY 3 cut(s) 108, 734, 1093
PscI ACATGT 1 cut(s) 768
PshAI GACNNNNGTC 1 cut(s) 749
Psp5II RGGWCCY 3 cut(s) 108, 734, 1093
Psp6I CCWGG 1 cut(s) 226
PspCI CACGTG 1 cut(s) 27
PspFI CCCAGC 2 cut(s) 1058, 1135
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 6 cut(s) 735, 736, 871, 1056, 1094, 1095
PspPI GGNCC 5 cut(s) 108, 734, 863, 1039, 1093
PspPPI RGGWCCY 3 cut(s) 108, 734, 1093
PsuI RGATCY 1 cut(s) 1054
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 4 cut(s) 170, 236, 255, 295
RsaNI GTAC 4 cut(s) 169, 235, 254, 294
RseI CAYNNNNRTG 1 cut(s) 68
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 560, 827, 891
SatI GCNGC 5 cut(s) 15, 385, 459, 576, 1156
Sau3AI GATC 6 cut(s) 630, 661, 937, 973, 1054, 1128
Sau96I GGNCC 5 cut(s) 108, 734, 863, 1039, 1093
SchI GAGTC 2 cut(s) 450, 1171
ScrFI CCNGG 4 cut(s) 228, 867, 1176, 1177
SduI GDGCHC 2 cut(s) 75, 621
SfaNI GCATC 1 cut(s) 484
Sfr274I CTCGAG 1 cut(s) 103
SgrAI CRCCGGYG 1 cut(s) 698
SinI GGWCC 5 cut(s) 108, 734, 863, 1039, 1093
SlaI CTCGAG 1 cut(s) 103
SmaI CCCGGG 1 cut(s) 1177
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 2 cut(s) 103, 826
SmoI CTYRAG 2 cut(s) 103, 826
SphI GCATGC 1 cut(s) 690
Sse9I AATT 4 cut(s) 19, 414, 477, 561
SsiI CCGC 4 cut(s) 330, 385, 1115, 1139
SspMI CTAG 3 cut(s) 719, 812, 1167
StyD4I CCNGG 4 cut(s) 226, 865, 1174, 1175
StyI CCWWGG 1 cut(s) 1166
TaaI ACNGT 7 cut(s) 130, 168, 280, 310, 361, 748, 1025
TaiI ACGT 4 cut(s) 29, 513, 780, 1080
TasI AATT 4 cut(s) 19, 414, 477, 561
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 2 cut(s) 120, 781
Tru1I TTAA 3 cut(s) 560, 827, 891
Tru9I TTAA 3 cut(s) 560, 827, 891
TscAI CASTG 2 cut(s) 435, 539
TseFI GTSAC 2 cut(s) 694, 897
TseI GCWGC 4 cut(s) 14, 458, 575, 1155
Tsp45I GTSAC 2 cut(s) 694, 897
TspDTI ATGAA 4 cut(s) 52, 112, 255, 980
TspGWI ACGGA 3 cut(s) 543, 816, 1014
TspMI CCCGGG 1 cut(s) 1175
TspRI CASTG 2 cut(s) 435, 539
Tth111I GACNNNGTC 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 826
VpaK11BI GGWCC 5 cut(s) 108, 734, 863, 1039, 1093
XapI RAATTY 1 cut(s) 477
XceI RCATGY 3 cut(s) 474, 690, 772
XhoI CTCGAG 1 cut(s) 103
XmaI CCCGGG 1 cut(s) 1175
XmaJI CCTAGG 1 cut(s) 1166
XmiI GTMKAC 2 cut(s) 132, 761
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 3 cut(s) 719, 812, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.