Rh6AG413900

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003654
Physical Location & Seq
Reverse (-)
60934097 .. 60935723
1627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG413900.1

Sequence Viewer

Length: 1287 bp
ATGGCGCGTAGGGCTCTAGTTAGAATGATTGTCATAATTTTGTATGCTTTCGTCGCTATTTCTCAAGCGGAAAATATAATAACAGTTGATCAATCCGGTAACGGTCATTACGGTAAAATACAAGATGCTATTGATTCTGTTCCGTCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGGACTTACAAAGAAAGTATTACCATACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAGAAGCAAGTGATGTGATGATAACGTGGGATCAGAGAGGGGACATTGATAAAGTTACAACATTTCATGTTTCGGCTTCCGACTTTGTAGCGCGCTACATAACAATTCAAAATACATTCGGGCCTGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAATAGCGTTTTATGACTGCTCAATTTTAGGTTATCAGGATACTCTATTTGACGAAATAGGCAAACACTACTATGAAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCGCAACGAAGAGAAGCGTCAACAGATAGTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAGAGGCGAAAAAACATTTTTAGGAAGACCATGGGAGGGATCCTATGCTAGGGTCATATTTGCCTACACTAACATGCAAGATGTGATAGAACCACTAGGTTGGTCTCGTTATTCTGATGGATCCACAGAACAAGAATTAATGACAAACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAGAATTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAGGATTGGATAAAGTCTACGCCGGTAACAACGTCGTCTCCACCTAAAAAGGAGACGCCGTCATTGCCTCCATCGGTACTGGATACACCGCCACCTCCACCACCGGTCATCCCGGCACCTGTAATCCCGTCTCCACCACCACAAGCCCCGGTGATTCCATTAGTTTCCCCACCAGCACCGGCAGTGATTATAACTCCGTGGTCGCCTCCACCAACATACGTGGACGGCAGAGCTCAAGAAGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGCATCCTCTTCAATCTTGAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTTACCTCGGCAGTTTTACTATACTTGTCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

428

Amino Acids

46.75

Weight (kDa)

5.46

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 308 5e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1115
AccB1I GGYRCC 1 cut(s) 1039
AccI GTMKAC 1 cut(s) 941
AccII CGCG 3 cut(s) 7, 343, 868
AciI CCGC 3 cut(s) 68, 1013, 1233
AclWI GGATC 7 cut(s) 152, 165, 288, 687, 700, 768, 781
AcsI RAATTY 2 cut(s) 872, 895
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 2 cut(s) 244, 1002
AfiI CCNNNNNNNGG 3 cut(s) 575, 689, 702
AgeI ACCGGT 1 cut(s) 1027
AgsI TTSAA 6 cut(s) 359, 503, 548, 820, 1212, 1219
AjnI CCWGG 2 cut(s) 181, 373
AluBI AGCT 1 cut(s) 1157
AluI AGCT 1 cut(s) 1157
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 5 cut(s) 406, 762, 966, 971, 1059
AlwI GGATC 7 cut(s) 152, 165, 288, 687, 700, 768, 781
Ama87I CYCGRG 1 cut(s) 1195
AoxI GGCC 2 cut(s) 371, 1281
ApoI RAATTY 2 cut(s) 872, 895
AseI ATTAAT 1 cut(s) 791
AsiGI ACCGGT 1 cut(s) 1027
Asp700I GAANNNNTTC 1 cut(s) 1226
AspLEI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 1037, 1073
AsuHPI GGTGA 2 cut(s) 1087, 1178
AvaI CYCGRG 1 cut(s) 1195
BamHI GGATCC 3 cut(s) 157, 692, 773
BanI GGYRCC 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 16, 1159
BbsI GAAGAC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 1159
BccI CCATC 2 cut(s) 764, 1003
BceAI ACGGC 2 cut(s) 967, 1165
BciT130I CCWGG 2 cut(s) 183, 375
BciVI GTATCC 2 cut(s) 445, 1000
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 1037, 1073
BcoDI GTCTC 5 cut(s) 406, 762, 966, 971, 1059
BfaI CTAG 4 cut(s) 17, 702, 749, 833
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 2 cut(s) 445, 1000
BisI GCNGC 1 cut(s) 1234
BlsI GCNGC 1 cut(s) 1235
Bme1390I CCNGG 4 cut(s) 183, 375, 1037, 1073
BmeT110I CYCGRG 1 cut(s) 1195
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 4 cut(s) 159, 694, 775, 1041
BmrFI CCNGG 4 cut(s) 183, 375, 1037, 1073
BmsI GCATC 2 cut(s) 115, 1211
BpiI GAAGAC 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 48, 1143
BpuMI CCSGG 2 cut(s) 1037, 1073
BsaAI YACGTR 1 cut(s) 1144
BsaHI GRCGYC 1 cut(s) 980
BsaI GGTCTC 2 cut(s) 406, 762
BsaJI CCNNGG 6 cut(s) 182, 683, 1071, 1121, 1194, 1257
BsaWI WCCGGW 2 cut(s) 95, 1027
BsaXI ACNNNNNCTCC 8 cut(s) 196, 226, 612, 642, 946, 968, 976, 998
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 689, 702
Bse118I RCCGGY 3 cut(s) 946, 1027, 1102
Bse1I ACTGG 3 cut(s) 534, 655, 1008
Bse3DI GCAATG 1 cut(s) 986
BseBI CCWGG 2 cut(s) 183, 375
BseDI CCNNGG 6 cut(s) 182, 683, 1071, 1121, 1194, 1257
BseGI GGATG 3 cut(s) 845, 1032, 1202
BseLI CCNNNNNNNGG 3 cut(s) 575, 689, 702
BseMI GCAATG 1 cut(s) 986
BseNI ACTGG 3 cut(s) 534, 655, 1008
BsePI GCGCGC 1 cut(s) 341
Bsh1236I CGCG 3 cut(s) 7, 343, 868
BshFI GGCC 2 cut(s) 373, 1283
BshNI GGYRCC 1 cut(s) 1039
BshTI ACCGGT 1 cut(s) 1027
BsiHKAI GWGCWC 1 cut(s) 1159
BsiHKCI CYCGRG 1 cut(s) 1195
BsiSI CCGG 7 cut(s) 96, 405, 947, 1028, 1037, 1073, 1103
BslFI GGGAC 3 cut(s) 199, 305, 1255
BslI CCNNNNNNNGG 3 cut(s) 575, 689, 702
BsmAI GTCTC 5 cut(s) 406, 762, 966, 971, 1059
BsmBI CGTCTC 3 cut(s) 966, 971, 1059
BsmFI GGGAC 3 cut(s) 199, 305, 1255
BsnI GGCC 2 cut(s) 373, 1283
Bso31I GGTCTC 2 cut(s) 406, 762
BsoBI CYCGRG 1 cut(s) 1195
Bsp1286I GDGCHC 2 cut(s) 16, 1159
Bsp143I GATC 6 cut(s) 88, 157, 174, 280, 692, 773
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 3 cut(s) 68, 1013, 1233
BspANI GGCC 2 cut(s) 373, 1283
BspFNI CGCG 3 cut(s) 7, 343, 868
BspLI GGNNCC 4 cut(s) 159, 694, 775, 1041
BspPI GGATC 7 cut(s) 152, 165, 288, 687, 700, 768, 781
BspT107I GGYRCC 1 cut(s) 1039
BspTNI GGTCTC 2 cut(s) 406, 762
BsrDI GCAATG 1 cut(s) 986
BsrFI RCCGGY 3 cut(s) 946, 1027, 1102
BsrI ACTGG 3 cut(s) 534, 655, 1008
BssAI RCCGGY 3 cut(s) 946, 1027, 1102
BssECI CCNNGG 6 cut(s) 182, 683, 1071, 1121, 1194, 1257
BssHII GCGCGC 1 cut(s) 341
BssMI GATC 6 cut(s) 88, 157, 174, 280, 692, 773
BssNI GRCGYC 1 cut(s) 980
BssT1I CCWWGG 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 183, 375
Bst4CI ACNGT 3 cut(s) 85, 104, 113
Bst6I CTCTTC 2 cut(s) 592, 1213
BstACI GRCGYC 1 cut(s) 980
BstBAI YACGTR 1 cut(s) 1144
BstC8I GCNNGC 2 cut(s) 343, 1248
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 2 cut(s) 683, 1121
BstENI CCTNNNNNAGG 2 cut(s) 573, 700
BstF5I GGATG 3 cut(s) 845, 1032, 1202
BstFNI CGCG 3 cut(s) 7, 343, 868
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
BstKTI GATC 6 cut(s) 91, 160, 177, 283, 695, 776
BstMAI GTCTC 5 cut(s) 406, 762, 966, 971, 1059
BstMBI GATC 6 cut(s) 88, 157, 174, 280, 692, 773
BstMWI GCNNNNNNNGC 3 cut(s) 11, 53, 988
BstNI CCWGG 2 cut(s) 183, 375
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 4 cut(s) 181, 373, 1035, 1071
BstSFI CTRYAG 2 cut(s) 249, 570
BstUI CGCG 3 cut(s) 7, 343, 868
BstV2I GAAGAC 1 cut(s) 685
BstX2I RGATCY 3 cut(s) 157, 692, 773
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 3 cut(s) 157, 692, 773
BsuI GTATCC 2 cut(s) 445, 1000
BsuRI GGCC 2 cut(s) 373, 1283
BtgI CCRYGG 2 cut(s) 683, 1121
BtsCI GGATG 3 cut(s) 845, 1032, 1202
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 2 cut(s) 883, 1113
Cac8I GCNNGC 2 cut(s) 343, 1248
CfoI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
Cfr10I RCCGGY 3 cut(s) 946, 1027, 1102
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 3 cut(s) 594, 988, 1252
Csp6I GTAC 2 cut(s) 243, 1001
CspAI ACCGGT 1 cut(s) 1027
CspCI CAANNNNNGTGG 2 cut(s) 1125, 1160
CviAII CATG 4 cut(s) 317, 684, 727, 1180
CviJI RGCY 9 cut(s) 14, 181, 223, 326, 373, 1070, 1157, 1250, 1283
CviKI_1 RGCY 9 cut(s) 14, 181, 223, 326, 373, 1070, 1157, 1250, 1283
CviQI GTAC 2 cut(s) 243, 1001
DdeI CTNAG 1 cut(s) 395
DpnI GATC 6 cut(s) 90, 159, 176, 282, 694, 775
DpnII GATC 6 cut(s) 88, 157, 174, 280, 692, 773
Eam1104I CTCTTC 2 cut(s) 592, 1213
EarI CTCTTC 2 cut(s) 592, 1213
Ecl136II GAGCTC 1 cut(s) 1157
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 16, 1159
Eco31I GGTCTC 2 cut(s) 406, 762
Eco53kI GAGCTC 1 cut(s) 1157
Eco88I CYCGRG 1 cut(s) 1195
EcoICRI GAGCTC 1 cut(s) 1157
EcoNI CCTNNNNNAGG 2 cut(s) 573, 700
EcoRI GAATTC 1 cut(s) 872
EcoRII CCWGG 2 cut(s) 181, 373
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 16, 1159
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 3 cut(s) 966, 971, 1059
FaeI CATG 4 cut(s) 320, 687, 730, 1183
FaqI GGGAC 3 cut(s) 199, 305, 1255
FatI CATG 4 cut(s) 316, 683, 726, 1179
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 941
Fnu4HI GCNGC 1 cut(s) 1234
FokI GGATG 3 cut(s) 832, 1019, 1189
FriOI GRGCYC 2 cut(s) 16, 1159
Fsp4HI GCNGC 1 cut(s) 1234
FspBI CTAG 4 cut(s) 17, 702, 749, 833
GlaI GCGC 7 cut(s) 6, 342, 344, 393, 532, 591, 867
GluI GCNGC 1 cut(s) 1234
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 2 cut(s) 373, 1283
HapII CCGG 7 cut(s) 96, 405, 947, 1028, 1037, 1073, 1103
HgaI GACGC 3 cut(s) 594, 988, 1252
HhaI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 4 cut(s) 320, 687, 730, 1183
Hin6I GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
HinP1I GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 2 cut(s) 134, 1078
HpaII CCGG 7 cut(s) 96, 405, 947, 1028, 1037, 1073, 1103
HphI GGTGA 2 cut(s) 1087, 1178
Hpy166II GTNNAC 5 cut(s) 609, 812, 942, 1147, 1169
Hpy188I TCNGA 3 cut(s) 285, 331, 769
Hpy188III TCNNGA 4 cut(s) 215, 449, 1160, 1216
Hpy8I GTNNAC 5 cut(s) 609, 812, 942, 1147, 1169
Hpy99I CGWCG 3 cut(s) 56, 830, 961
HpyAV CCTTC 2 cut(s) 497, 1157
HpyCH4III ACNGT 3 cut(s) 85, 104, 113
HpyCH4IV ACGT 3 cut(s) 275, 956, 1143
HpyCH4V TGCA 2 cut(s) 730, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 53, 988
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 3 cut(s) 275, 956, 1143
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 4 cut(s) 320, 687, 730, 1183
HspAI GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 88, 157, 174, 280, 692, 773
LmnI GCTCC 1 cut(s) 836
LweI GCATC 2 cut(s) 115, 1211
MaeI CTAG 4 cut(s) 17, 702, 749, 833
MaeII ACGT 3 cut(s) 275, 956, 1143
MaeIII GTNAC 5 cut(s) 98, 304, 494, 554, 949
MalI GATC 6 cut(s) 90, 159, 176, 282, 694, 775
MboI GATC 6 cut(s) 88, 157, 174, 280, 692, 773
MboII GAAGA 4 cut(s) 609, 690, 1200, 1231
MflI RGATCY 3 cut(s) 157, 692, 773
MhlI GDGCHC 2 cut(s) 16, 1159
MmeI TCCRAC 2 cut(s) 354, 873
MroXI GAANNNNTTC 1 cut(s) 1226
MseI TTAA 4 cut(s) 236, 636, 791, 858
MslI CAYNNNNRTG 2 cut(s) 483, 725
MspA1I CMGCKG 1 cut(s) 1233
MspI CCGG 7 cut(s) 96, 405, 947, 1028, 1037, 1073, 1103
MspR9I CCNGG 4 cut(s) 183, 375, 1037, 1073
MvaI CCWGG 2 cut(s) 183, 375
MvnI CGCG 3 cut(s) 7, 343, 868
MwoI GCNNNNNNNGC 3 cut(s) 11, 53, 988
NciI CCSGG 2 cut(s) 1037, 1073
NcoI CCATGG 1 cut(s) 683
NdeII GATC 6 cut(s) 88, 157, 174, 280, 692, 773
NlaIII CATG 4 cut(s) 320, 687, 730, 1183
NlaIV GGNNCC 4 cut(s) 159, 694, 775, 1041
NmeAIII GCCGAG 1 cut(s) 1238
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 1 cut(s) 730
PauI GCGCGC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 1226
PfeI GAWTC 2 cut(s) 134, 1078
PflFI GACNNNGTC 1 cut(s) 982
PinAI ACCGGT 1 cut(s) 1027
PkrI GCNGC 1 cut(s) 1235
Ppu21I YACGTR 1 cut(s) 1144
PshBI ATTAAT 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1115
Psp124BI GAGCTC 1 cut(s) 1159
Psp6I CCWGG 2 cut(s) 181, 373
PspGI CCWGG 2 cut(s) 181, 373
PspN4I GGNNCC 4 cut(s) 159, 694, 775, 1041
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 3 cut(s) 157, 692, 773
PsyI GACNNNGTC 1 cut(s) 982
PteI GCGCGC 1 cut(s) 341
RsaI GTAC 2 cut(s) 244, 1002
RsaNI GTAC 2 cut(s) 243, 1001
RseI CAYNNNNRTG 2 cut(s) 483, 725
SacI GAGCTC 1 cut(s) 1159
SaqAI TTAA 4 cut(s) 236, 636, 791, 858
SatI GCNGC 1 cut(s) 1234
Sau3AI GATC 6 cut(s) 88, 157, 174, 280, 692, 773
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 4 cut(s) 183, 375, 1037, 1073
SduI GDGCHC 2 cut(s) 16, 1159
SfaNI GCATC 2 cut(s) 115, 1211
SfcI CTRYAG 2 cut(s) 249, 570
SmiMI CAYNNNNRTG 2 cut(s) 483, 725
SmlI CTYRAG 2 cut(s) 63, 1158
SmoI CTYRAG 2 cut(s) 63, 1158
SsiI CCGC 3 cut(s) 68, 1013, 1233
SspMI CTAG 4 cut(s) 17, 702, 749, 833
SstI GAGCTC 1 cut(s) 1159
StyD4I CCNGG 4 cut(s) 181, 373, 1035, 1071
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 3 cut(s) 85, 104, 113
TaiI ACGT 3 cut(s) 278, 959, 1146
TaqI TCGA 1 cut(s) 173
TauI GCSGC 1 cut(s) 1236
TfiI GAWTC 2 cut(s) 134, 1078
Tru1I TTAA 4 cut(s) 236, 636, 791, 858
Tru9I TTAA 4 cut(s) 236, 636, 791, 858
TscAI CASTG 2 cut(s) 883, 1113
TseFI GTSAC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 3 cut(s) 217, 305, 501
TspGWI ACGGA 3 cut(s) 132, 843, 1110
TspRI CASTG 2 cut(s) 883, 1113
Tth111I GACNNNGTC 1 cut(s) 982
VspI ATTAAT 1 cut(s) 791
XagI CCTNNNNNAGG 2 cut(s) 573, 700
XapI RAATTY 2 cut(s) 872, 895
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 1226
XspI CTAG 4 cut(s) 17, 702, 749, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.