Rroxscaffold_7G00167260

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003654
Physical Location & Seq
Forward (+)
8463608 .. 8465418
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167260.1

Sequence Viewer

Length: 1743 bp
ATGGCGCGTATGGCTCTAGTTAGAATGATTAACGGTCATTACGGTAAAATACAAGATGCTATTGATTCTATTCCGTCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGAACTTACAAAGAAAGTATAACCATACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAAAAGCAAGTGATGTGATGGTAACGTGGGATCGTAGAGGGGACATCGATAAAGTTGCAACATTTCATGTTTCGCTTCGATTTCGTAGCGCGCTACGTAACAATTCAAAATACATTCGGCCTGGTCATCAAGCAATTGCGCTAAGGGTCTCCGGTGATAGAATAGCATTTTATGACTGCTCAATTTTAGGTTATCAGGATACTCTATTTGATGAAATAGGCAGACACTACTACGAAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCACAACGGAGAGAAGCGTCAACAGACAGTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAAAGGCGAAAAAACATTTTTAGGAAGACCATGGGAGGGATCCTATGCTAGGGTCATATTTGCCTACACTAACATGGAAGATGTGATAGAACCACTAGGTTGGTCTCGTTATTCTGATGGATCCACAGAACAAGAACTAATGACAGACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAAAATTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAAGATTGGATAAAGTCTACGCCGGTAACAGCGTCGCCTCCACCATCACCTCAACCTAAAAAAGAGACGCCGTCATTGCCTCCATCGGTACCGGATACACCGCCACCCTCACCACCACCTCCCATGAAGCCGTCTGCACATCCACCAGCACCGGTCTTGCCACCCTCACCACCACCCCCCATGAAGCCGTCTGCACCACCACCAGCAGCGGTCATCCCGGCGGCACCTGTAATCCCGTCTCCACCACCACAAGCCCCGGTGGTACCAACTTCCCCACCACCGGTGATTCCATTAGTTTCCCCACCAGCACCGGCAGTGATTCCATCGTCGCCACCACTAGCACCCGTGGTGATTTTATCGCCGCCAGCACCAGCACCAGCACCCGTCATCCCGTCGTCGCCACCACCGACAACGGCACCACCAGTGATTCCGTTGTTGCCCCCACCGACACCGGTGGTGATTCCATCGCCGCCCCCACCAGCACCGGCGGCGATCCCGTCGTCGCCACCATCGACACCCGTGACCCCGTGGTTCCCGTGGTTCGCTCCACCGGCACCGGTGATACCGACGTTGCCACCACTGGTAAATCCATTTTCACCACCACCGGTACCGATGATTCCGTTGTTGCCCCCACGGGCACCGGTGGTGATCTTGCCACCAGCGACACCCGTGATCTCGTGGTTCGCACCACCGCCACCATCACCGCCGAGGGTGATTATAACTCCGTGGTTGCCTCCACCAAGATACATGGACGGCAGAGCTCAAGAAGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGAATCCTCTTCGATCTTTAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTAACCTCGGCAGTTTTACTGTACTTGTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

580

Amino Acids

62.22

Weight (kDa)

8.8

Isoelectric Point (pI)

73.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 11 - 284 6e-54 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1571
Acc65I GGTACC 3 cut(s) 940, 1114, 1459
AccB1I GGYRCC 7 cut(s) 940, 1075, 1114, 1267, 1405, 1459, 1489
AccI GTMKAC 1 cut(s) 869
AccII CGCG 3 cut(s) 7, 272, 796
AciI CCGC 9 cut(s) 953, 1061, 1073, 1214, 1322, 1340, 1544, 1556, 1689
AclWI GGATC 8 cut(s) 83, 96, 219, 615, 628, 696, 709, 1339
AcsI RAATTY 2 cut(s) 800, 823
AcyI GRCGYC 1 cut(s) 920
AfaI GTAC 5 cut(s) 175, 942, 1116, 1461, 1730
AfiI CCNNNNNNNGG 4 cut(s) 503, 617, 630, 1132
AgeI ACCGGT 6 cut(s) 1003, 1132, 1303, 1408, 1456, 1492
AgsI TTSAA 4 cut(s) 288, 431, 476, 748
AjnI CCWGG 2 cut(s) 112, 301
AluBI AGCT 1 cut(s) 1613
AluI AGCT 1 cut(s) 1613
Alw21I GWGCWC 1 cut(s) 1615
Alw26I GTCTC 4 cut(s) 334, 690, 911, 1095
AlwI GGATC 8 cut(s) 83, 96, 219, 615, 628, 696, 709, 1339
Ama87I CYCGRG 1 cut(s) 1651
AoxI GGCC 2 cut(s) 299, 1737
ApeKI GCWGC 1 cut(s) 1058
ApoI RAATTY 2 cut(s) 800, 823
AsiGI ACCGGT 6 cut(s) 1003, 1132, 1303, 1408, 1456, 1492
Asp700I GAANNNNTTC 1 cut(s) 1682
Asp718I GGTACC 3 cut(s) 940, 1114, 1459
AspLEI GCGC 6 cut(s) 7, 272, 274, 322, 461, 796
AsuC2I CCSGG 2 cut(s) 1070, 1109
AvaI CYCGRG 1 cut(s) 1651
BaeGI GKGCMC 1 cut(s) 1492
BaeI ACNNNNGTAYC 2 cut(s) 1406, 1439
BamHI GGATCC 3 cut(s) 88, 620, 701
BanI GGYRCC 7 cut(s) 940, 1075, 1114, 1267, 1405, 1459, 1489
BanII GRGCYC 1 cut(s) 1615
BauI CACGAG 1 cut(s) 1528
BbsI GAAGAC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 1615
BbvI GCAGC 1 cut(s) 1070
BccI CCATC 8 cut(s) 193, 692, 904, 943, 1183, 1324, 1369, 1558
BceAI ACGGC 5 cut(s) 907, 967, 1024, 1281, 1621
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciT130I CCWGG 2 cut(s) 114, 303
BciVI GTATCC 2 cut(s) 373, 940
BcnI CCSGG 2 cut(s) 1070, 1109
BcoDI GTCTC 4 cut(s) 334, 690, 911, 1095
BfaI CTAG 5 cut(s) 17, 630, 677, 761, 1190
BfmI CTRYAG 1 cut(s) 498
BfoI RGCGCY 1 cut(s) 462
BfuI GTATCC 2 cut(s) 373, 940
BisI GCNGC 6 cut(s) 1059, 1074, 1214, 1322, 1341, 1690
BlsI GCNGC 6 cut(s) 1060, 1075, 1215, 1323, 1342, 1691
Bme1390I CCNGG 4 cut(s) 114, 303, 1070, 1109
BmeT110I CYCGRG 1 cut(s) 1651
BmrFI CCNGG 4 cut(s) 114, 303, 1070, 1109
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 613
BpmI CTGGAG 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 1599
BpuMI CCSGG 2 cut(s) 1070, 1109
Bsa29I ATCGAT 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 920
BsaI GGTCTC 2 cut(s) 334, 690
BsaWI WCCGGW 8 cut(s) 332, 943, 1003, 1132, 1303, 1408, 1456, 1492
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 540, 570
Bsc4I CCNNNNNNNGG 4 cut(s) 503, 617, 630, 1132
Bse1I ACTGG 4 cut(s) 462, 583, 1274, 1437
Bse3DI GCAATG 1 cut(s) 926
BseBI CCWGG 2 cut(s) 114, 303
BseCI ATCGAT 1 cut(s) 228
BseGI GGATG 4 cut(s) 773, 991, 1065, 1239
BseLI CCNNNNNNNGG 4 cut(s) 503, 617, 630, 1132
BseMI GCAATG 1 cut(s) 926
BseNI ACTGG 4 cut(s) 462, 583, 1274, 1437
BsePI GCGCGC 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 1492
BseXI GCAGC 1 cut(s) 1070
BsgI GTGCAG 2 cut(s) 972, 1029
Bsh1236I CGCG 3 cut(s) 7, 272, 796
BshFI GGCC 2 cut(s) 301, 1739
BshNI GGYRCC 7 cut(s) 940, 1075, 1114, 1267, 1405, 1459, 1489
BshTI ACCGGT 6 cut(s) 1003, 1132, 1303, 1408, 1456, 1492
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 1615
BsiHKCI CYCGRG 1 cut(s) 1651
BslFI GGGAC 2 cut(s) 236, 1711
BslI CCNNNNNNNGG 4 cut(s) 503, 617, 630, 1132
BsmAI GTCTC 4 cut(s) 334, 690, 911, 1095
BsmBI CGTCTC 2 cut(s) 911, 1095
BsmFI GGGAC 2 cut(s) 236, 1711
BsnI GGCC 2 cut(s) 301, 1739
Bso31I GGTCTC 2 cut(s) 334, 690
BsoBI CYCGRG 1 cut(s) 1651
Bsp1286I GDGCHC 2 cut(s) 1492, 1615
Bsp143I GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
Bsp19I CCATGG 1 cut(s) 611
BspACI CCGC 9 cut(s) 953, 1061, 1073, 1214, 1322, 1340, 1544, 1556, 1689
BspANI GGCC 2 cut(s) 301, 1739
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 3 cut(s) 7, 272, 796
BspPI GGATC 8 cut(s) 83, 96, 219, 615, 628, 696, 709, 1339
BspT107I GGYRCC 7 cut(s) 940, 1075, 1114, 1267, 1405, 1459, 1489
BspTNI GGTCTC 2 cut(s) 334, 690
BsrDI GCAATG 1 cut(s) 926
BsrI ACTGG 4 cut(s) 462, 583, 1274, 1437
BssHII GCGCGC 1 cut(s) 270
BssMI GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
BssNI GRCGYC 1 cut(s) 920
BssSI CACGAG 1 cut(s) 1528
BssT1I CCWWGG 1 cut(s) 611
Bst2BI CACGAG 1 cut(s) 1528
Bst2UI CCWGG 2 cut(s) 114, 303
Bst4CI ACNGT 4 cut(s) 35, 44, 545, 1728
Bst6I CTCTTC 1 cut(s) 1669
BstACI GRCGYC 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 272, 1218, 1704
BstDEI CTNAG 1 cut(s) 323
BstDSI CCRYGG 6 cut(s) 611, 1197, 1379, 1388, 1484, 1577
BstENI CCTNNNNNAGG 2 cut(s) 501, 628
BstF5I GGATG 4 cut(s) 773, 991, 1065, 1239
BstFNI CGCG 3 cut(s) 7, 272, 796
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 6 cut(s) 7, 272, 274, 322, 461, 796
BstKTI GATC 9 cut(s) 91, 108, 214, 623, 704, 1347, 1503, 1527, 1671
BstMAI GTCTC 4 cut(s) 334, 690, 911, 1095
BstMBI GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
BstMWI GCNNNNNNNGC 4 cut(s) 11, 928, 1340, 1403
BstNI CCWGG 2 cut(s) 114, 303
BstSCI CCNGG 4 cut(s) 112, 301, 1068, 1107
BstSFI CTRYAG 1 cut(s) 498
BstSLI GKGCMC 1 cut(s) 1492
BstSNI TACGTA 1 cut(s) 278
BstUI CGCG 3 cut(s) 7, 272, 796
BstV1I GCAGC 1 cut(s) 1070
BstV2I GAAGAC 1 cut(s) 613
BstX2I RGATCY 3 cut(s) 88, 620, 701
BstXI CCANNNNNNTGG 1 cut(s) 681
BstYI RGATCY 3 cut(s) 88, 620, 701
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 2 cut(s) 373, 940
BsuRI GGCC 2 cut(s) 301, 1739
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 6 cut(s) 611, 1197, 1379, 1388, 1484, 1577
BtgZI GCGATG 1 cut(s) 1302
BtsCI GGATG 4 cut(s) 773, 991, 1065, 1239
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 5 cut(s) 550, 811, 1173, 1281, 1430
Cac8I GCNNGC 3 cut(s) 272, 1218, 1704
CfoI GCGC 6 cut(s) 7, 272, 274, 322, 461, 796
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 4 cut(s) 522, 873, 928, 1708
Csp6I GTAC 5 cut(s) 174, 941, 1115, 1460, 1729
CspAI ACCGGT 6 cut(s) 1003, 1132, 1303, 1408, 1456, 1492
CviAII CATG 7 cut(s) 248, 612, 655, 976, 1033, 1600, 1636
CviQI GTAC 5 cut(s) 174, 941, 1115, 1460, 1729
DdeI CTNAG 1 cut(s) 323
DpnI GATC 9 cut(s) 90, 107, 213, 622, 703, 1346, 1502, 1526, 1670
DpnII GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
Eam1104I CTCTTC 1 cut(s) 1669
EarI CTCTTC 1 cut(s) 1669
Ecl136II GAGCTC 1 cut(s) 1613
Eco105I TACGTA 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 1615
Eco31I GGTCTC 2 cut(s) 334, 690
Eco53kI GAGCTC 1 cut(s) 1613
Eco88I CYCGRG 1 cut(s) 1651
EcoICRI GAGCTC 1 cut(s) 1613
EcoNI CCTNNNNNAGG 2 cut(s) 501, 628
EcoRII CCWGG 2 cut(s) 112, 301
EcoT14I CCWWGG 1 cut(s) 611
EcoT38I GRGCYC 1 cut(s) 1615
ErhI CCWWGG 1 cut(s) 611
Esp3I CGTCTC 2 cut(s) 911, 1095
FaeI CATG 7 cut(s) 251, 615, 658, 979, 1036, 1603, 1639
FaqI GGGAC 2 cut(s) 236, 1711
FatI CATG 7 cut(s) 247, 611, 654, 975, 1032, 1599, 1635
FblI GTMKAC 1 cut(s) 869
Fnu4HI GCNGC 6 cut(s) 1059, 1074, 1214, 1322, 1341, 1690
FokI GGATG 4 cut(s) 760, 978, 1052, 1226
FriOI GRGCYC 1 cut(s) 1615
Fsp4HI GCNGC 6 cut(s) 1059, 1074, 1214, 1322, 1341, 1690
FspBI CTAG 5 cut(s) 17, 630, 677, 761, 1190
GlaI GCGC 6 cut(s) 6, 271, 273, 321, 460, 795
GluI GCNGC 6 cut(s) 1059, 1074, 1214, 1322, 1341, 1690
GsuI CTGGAG 1 cut(s) 129
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 2 cut(s) 301, 1739
HgaI GACGC 4 cut(s) 522, 873, 928, 1708
HhaI GCGC 6 cut(s) 7, 272, 274, 322, 461, 796
Hin1I GRCGYC 1 cut(s) 920
Hin1II CATG 7 cut(s) 251, 615, 658, 979, 1036, 1603, 1639
Hin6I GCGC 6 cut(s) 5, 270, 272, 320, 459, 794
HinP1I GCGC 6 cut(s) 5, 270, 272, 320, 459, 794
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HinfI GANTC 8 cut(s) 65, 728, 1138, 1171, 1279, 1312, 1468, 1658
Hpy166II GTNNAC 4 cut(s) 537, 740, 870, 1625
Hpy188I TCNGA 1 cut(s) 697
Hpy188III TCNNGA 3 cut(s) 146, 377, 1616
Hpy8I GTNNAC 4 cut(s) 537, 740, 870, 1625
Hpy99I CGWCG 8 cut(s) 758, 889, 1183, 1249, 1252, 1354, 1357, 1423
HpyAV CCTTC 2 cut(s) 425, 1613
HpyCH4III ACNGT 4 cut(s) 35, 44, 545, 1728
HpyCH4IV ACGT 3 cut(s) 206, 277, 1421
HpyCH4V TGCA 4 cut(s) 239, 821, 989, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 928, 1340, 1403
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 3 cut(s) 206, 277, 1421
Hsp92I GRCGYC 1 cut(s) 920
Hsp92II CATG 7 cut(s) 251, 615, 658, 979, 1036, 1603, 1639
HspAI GCGC 6 cut(s) 5, 270, 272, 320, 459, 794
KpnI GGTACC 3 cut(s) 944, 1118, 1463
Kzo9I GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
LmnI GCTCC 2 cut(s) 764, 1402
Lsp1109I GCAGC 1 cut(s) 1070
LweI GCATC 1 cut(s) 46
MaeI CTAG 5 cut(s) 17, 630, 677, 761, 1190
MaeII ACGT 3 cut(s) 206, 277, 1421
MaeIII GTNAC 6 cut(s) 202, 278, 422, 482, 877, 1372
MalI GATC 9 cut(s) 90, 107, 213, 622, 703, 1346, 1502, 1526, 1670
MboI GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
MboII GAAGA 3 cut(s) 618, 671, 1656
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 3 cut(s) 88, 620, 701
MhlI GDGCHC 2 cut(s) 1492, 1615
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 1 cut(s) 801
MroXI GAANNNNTTC 1 cut(s) 1682
MseI TTAA 5 cut(s) 30, 167, 564, 786, 1674
MslI CAYNNNNRTG 1 cut(s) 653
MspA1I CMGCKG 2 cut(s) 1061, 1689
MspR9I CCNGG 4 cut(s) 114, 303, 1070, 1109
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 2 cut(s) 114, 303
MvnI CGCG 3 cut(s) 7, 272, 796
MwoI GCNNNNNNNGC 4 cut(s) 11, 928, 1340, 1403
NciI CCSGG 2 cut(s) 1070, 1109
NcoI CCATGG 1 cut(s) 611
NdeII GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
NlaIII CATG 7 cut(s) 251, 615, 658, 979, 1036, 1603, 1639
NmeAIII GCCGAG 2 cut(s) 1584, 1694
NmuCI GTSAC 2 cut(s) 482, 1372
PauI GCGCGC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 2 cut(s) 1256, 1361
PdmI GAANNNNTTC 1 cut(s) 1682
PfeI GAWTC 7 cut(s) 65, 1138, 1171, 1279, 1312, 1468, 1658
PflFI GACNNNGTC 1 cut(s) 922
PinAI ACCGGT 6 cut(s) 1003, 1132, 1303, 1408, 1456, 1492
PkrI GCNGC 6 cut(s) 1060, 1075, 1215, 1323, 1342, 1691
PleI GAGTC 1 cut(s) 722
PpsI GAGTC 1 cut(s) 722
Ppu21I YACGTR 1 cut(s) 278
PsiI TTATAA 1 cut(s) 1571
Psp124BI GAGCTC 1 cut(s) 1615
Psp6I CCWGG 2 cut(s) 112, 301
PspGI CCWGG 2 cut(s) 112, 301
PsuI RGATCY 3 cut(s) 88, 620, 701
PsyI GACNNNGTC 1 cut(s) 922
PteI GCGCGC 1 cut(s) 270
RsaI GTAC 5 cut(s) 175, 942, 1116, 1461, 1730
RsaNI GTAC 5 cut(s) 174, 941, 1115, 1460, 1729
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 1615
SaqAI TTAA 5 cut(s) 30, 167, 564, 786, 1674
SatI GCNGC 6 cut(s) 1059, 1074, 1214, 1322, 1341, 1690
Sau3AI GATC 9 cut(s) 88, 105, 211, 620, 701, 1344, 1500, 1524, 1668
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 4 cut(s) 114, 303, 1070, 1109
SduI GDGCHC 2 cut(s) 1492, 1615
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 498
SgrAI CRCCGGYG 5 cut(s) 1132, 1303, 1336, 1408, 1492
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 1614
SmoI CTYRAG 1 cut(s) 1614
SnaBI TACGTA 1 cut(s) 278
SsiI CCGC 9 cut(s) 953, 1061, 1073, 1214, 1322, 1340, 1544, 1556, 1689
SspMI CTAG 5 cut(s) 17, 630, 677, 761, 1190
SstI GAGCTC 1 cut(s) 1615
StyD4I CCNGG 4 cut(s) 112, 301, 1068, 1107
StyI CCWWGG 1 cut(s) 611
TaaI ACNGT 4 cut(s) 35, 44, 545, 1728
TaiI ACGT 3 cut(s) 209, 280, 1424
TaqI TCGA 5 cut(s) 104, 228, 259, 1364, 1667
TatI WGTACW 1 cut(s) 1728
TauI GCSGC 5 cut(s) 1076, 1216, 1324, 1343, 1692
TfiI GAWTC 7 cut(s) 65, 1138, 1171, 1279, 1312, 1468, 1658
Tru1I TTAA 5 cut(s) 30, 167, 564, 786, 1674
Tru9I TTAA 5 cut(s) 30, 167, 564, 786, 1674
TscAI CASTG 5 cut(s) 550, 811, 1173, 1281, 1437
TseFI GTSAC 2 cut(s) 482, 1372
TseI GCWGC 1 cut(s) 1058
Tsp45I GTSAC 2 cut(s) 482, 1372
TspDTI ATGAA 5 cut(s) 148, 236, 408, 992, 1049
TspGWI ACGGA 6 cut(s) 63, 538, 771, 1272, 1461, 1566
TspRI CASTG 5 cut(s) 550, 811, 1173, 1281, 1437
Tth111I GACNNNGTC 1 cut(s) 922
XagI CCTNNNNNAGG 2 cut(s) 501, 628
XapI RAATTY 2 cut(s) 800, 823
XmiI GTMKAC 1 cut(s) 869
XmnI GAANNNNTTC 1 cut(s) 1682
XspI CTAG 5 cut(s) 17, 630, 677, 761, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.