FvH4_2g39830

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003654
Physical Location & Seq
Forward (+)
28496820 .. 28498238
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39830.t1

Sequence Viewer

Length: 1419 bp
ATGGCTCGTGGTGCTCCAGTTAGCATGATTCTCATAATCTTGTTTGCTTTCATCTCTGTTTCTCATGCCGGAAATATCATAACGGTGGATCAGTCCGGTCACGGTGATTTCTACAAAATACAAGACGCTATCAATTCTATTCCGGCAAACAATAAGGAGGCTGTGACAATTTCGATCAATCCTGGAAAATACAAGGAGACTATCACCGTACCTCCAGACAAGCCATTCATAACATTAAGAGGTAGCACCACAAAACCAAGCGACGTGATAATTACGTGGGATCAAAGTGGCGACATTGTTACTGTTGCGACATTTCATGTTTTGGCTTCCGACTTCATAGCTCGCGACGTAACGTTTCAAAACACATTCGGGCATGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAGTATCGTTTTACGACTGCTCGATTATCGGTTACCAAGATACGCTATTCGACGAAACCGGCAGACATTACTACGAAAATTGTTACATTGAAGGAGTCGTTGATTTCATATTCGGAAGCGCCACCTCTTTCTTTCACAGTTGTCACATACATTCGGCAAGAGCAGGATTTATTACGGCGCAGCGAAGGGAAACGCCGACTGATAATGGAGGGTTCGTGTTCTTTAAGGGTTCGATTACGGGCGAAGGTGGAAAAACTTATTTAGGAAGACCATGGGTGGGTTCTTATGCTAGGGTCATCTTCGCCTACACTAACATGGAGAATGTGATAGAACCAGCAGGTTGGTCTGATGATGGAATCGGAGAAAAAGAATTGAAGGCGAAGTCGTTTTGTGGGCAGTTCAAATGCGACGGACCAGGAGCAACAACGTCCGAGAGGGTTCAATGGGCGCGAGAGTTCAGTGAGGAAGAAATTTCAAAGTTTGTGACGACAGATTTTATCAATGGAAAAGATTGGTTAAAGTCTAGTACTCCGGTAACAACTCCACCTCCGCCGTCTGCACCACCTCCCATGAAGCCATCTTCGCCACCACCGGCACCGGTGGTGATTCCATCGTCTCCTCCTCCGGCACCAGAGATGAAGCCATCTTCACCACCACGTCTCATGAAGCCATCTTCGCCCCCGCCGCCACCGGTGGTGATTCCCTCCTCGCCGCCACCAGCACCGGTGACACAGCTGTCGTCGTCACCACCACCGCCATTACCATTGAGGGTAATCATCACTCCGTTTTCGCCTCCATCAACATCGGTGAATAGTGGAGGGAAAGAAGGTGAACCTATCATCATGCTTTACTACTCAGGGGCTTCTTCACACTCTAAGCATCGTTTCAGCACCGGTGGGATACTGGCTCTAGCAGCAGGTTTGGTAGCCGCAGCAGGTTTGGGGGTATGGCTCTACAGAAGGCGCAGAACATTGAACGAGAAGCTGAACCGCTCCTGCTTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

473

Amino Acids

50.95

Weight (kDa)

8.08

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 306 2.8e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1147
Acc36I ACCTGC 3 cut(s) 740, 1319, 1337
AccB1I GGYRCC 2 cut(s) 1006, 1039
AccBSI CCGCTC 1 cut(s) 1404
AccII CGCG 2 cut(s) 345, 862
AciI CCGC 7 cut(s) 962, 1094, 1097, 1124, 1166, 1341, 1402
AclI AACGTT 1 cut(s) 353
AclWI GGATC 2 cut(s) 96, 288
AcsI RAATTY 1 cut(s) 882
AfaI GTAC 2 cut(s) 210, 940
AfiI CCNNNNNNNGG 1 cut(s) 689
AgeI ACCGGT 4 cut(s) 1009, 1102, 1135, 1304
AgsI TTSAA 7 cut(s) 359, 503, 787, 814, 854, 888, 1387
AjiI CACGTC 2 cut(s) 265, 1070
AjnI CCWGG 2 cut(s) 181, 826
AluBI AGCT 3 cut(s) 341, 1147, 1396
AluI AGCT 3 cut(s) 341, 1147, 1396
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 4 cut(s) 191, 406, 1032, 1076
AlwI GGATC 2 cut(s) 96, 288
ApeKI GCWGC 3 cut(s) 592, 1325, 1343
ApoI RAATTY 1 cut(s) 882
AsiGI ACCGGT 4 cut(s) 1009, 1102, 1135, 1304
AspLEI GCGC 5 cut(s) 394, 533, 592, 862, 1377
AspS9I GGNCC 1 cut(s) 824
AsuHPI GGTGA 9 cut(s) 116, 196, 1027, 1053, 1120, 1149, 1150, 1231, 1253
AvaII GGWCC 1 cut(s) 824
BanI GGYRCC 2 cut(s) 1006, 1039
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 604, 1337, 1355
BccI CCATC 6 cut(s) 758, 997, 1030, 1063, 1090, 1216
BceAI ACGGC 2 cut(s) 603, 949
BciT130I CCWGG 2 cut(s) 183, 828
BciVI GTATCC 1 cut(s) 1305
BcoDI GTCTC 4 cut(s) 191, 406, 1032, 1076
BfaI CTAG 4 cut(s) 702, 936, 1322, 1417
BfmI CTRYAG 1 cut(s) 1366
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 3 cut(s) 740, 1319, 1337
BfuI GTATCC 1 cut(s) 1305
BisI GCNGC 6 cut(s) 593, 1097, 1124, 1326, 1341, 1344
BlsI GCNGC 6 cut(s) 594, 1098, 1125, 1327, 1342, 1345
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 2 cut(s) 183, 828
Bme18I GGWCC 1 cut(s) 824
BmgBI CACGTC 2 cut(s) 265, 1070
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 2 cut(s) 1008, 1041
BmrFI CCNGG 2 cut(s) 183, 828
BmsI GCATC 1 cut(s) 1300
BpiI GAAGAC 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 683
BsaWI WCCGGW 6 cut(s) 95, 943, 1009, 1102, 1135, 1304
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 612, 642, 943, 973
Bsc4I CCNNNNNNNGG 1 cut(s) 689
Bse118I RCCGGY 6 cut(s) 470, 1003, 1009, 1102, 1135, 1304
Bse1I ACTGG 2 cut(s) 17, 1320
BseBI CCWGG 2 cut(s) 183, 828
BseDI CCNNGG 1 cut(s) 683
BseLI CCNNNNNNNGG 1 cut(s) 689
BseMII CTCAG 1 cut(s) 1281
BseNI ACTGG 2 cut(s) 17, 1320
BseRI GAGGAG 3 cut(s) 1020, 1023, 1108
BseXI GCAGC 3 cut(s) 604, 1337, 1355
BsgI GTGCAG 1 cut(s) 954
Bsh1236I CGCG 2 cut(s) 345, 862
BshNI GGYRCC 2 cut(s) 1006, 1039
BshTI ACCGGT 4 cut(s) 1009, 1102, 1135, 1304
BsiHKAI GWGCWC 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 689
BsmAI GTCTC 4 cut(s) 191, 406, 1032, 1076
BsmBI CGTCTC 2 cut(s) 1032, 1076
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 3 cut(s) 88, 174, 280
Bsp19I CCATGG 1 cut(s) 683
Bsp68I TCGCGA 1 cut(s) 345
BspACI CCGC 7 cut(s) 962, 1094, 1097, 1124, 1166, 1341, 1402
BspCNI CTCAG 1 cut(s) 1280
BspFNI CGCG 2 cut(s) 345, 862
BspHI TCATGA 1 cut(s) 1074
BspLI GGNNCC 2 cut(s) 1008, 1041
BspMI ACCTGC 3 cut(s) 740, 1319, 1337
BspPI GGATC 2 cut(s) 96, 288
BspT107I GGYRCC 2 cut(s) 1006, 1039
BspTNI GGTCTC 1 cut(s) 406
BsrBI CCGCTC 1 cut(s) 1404
BsrFI RCCGGY 6 cut(s) 470, 1003, 1009, 1102, 1135, 1304
BsrI ACTGG 2 cut(s) 17, 1320
BssAI RCCGGY 6 cut(s) 470, 1003, 1009, 1102, 1135, 1304
BssECI CCNNGG 1 cut(s) 683
BssMI GATC 3 cut(s) 88, 174, 280
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 183, 828
Bst4CI ACNGT 5 cut(s) 85, 104, 208, 304, 551
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 395, 1267, 1287
BstDSI CCRYGG 1 cut(s) 683
BstEII GGTNACC 1 cut(s) 443
BstFNI CGCG 2 cut(s) 345, 862
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 5 cut(s) 394, 533, 592, 862, 1377
BstKTI GATC 3 cut(s) 91, 177, 283
BstMAI GTCTC 4 cut(s) 191, 406, 1032, 1076
BstMBI GATC 3 cut(s) 88, 174, 280
BstMWI GCNNNNNNNGC 5 cut(s) 11, 994, 1087, 1096, 1325
BstNI CCWGG 2 cut(s) 183, 828
BstPI GGTNACC 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 181, 826
BstSFI CTRYAG 1 cut(s) 1366
BstUI CGCG 2 cut(s) 345, 862
BstV1I GCAGC 3 cut(s) 604, 1337, 1355
BstV2I GAAGAC 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 753
BsuI GTATCC 1 cut(s) 1305
BtgI CCRYGG 1 cut(s) 683
BtrI CACGTC 2 cut(s) 265, 1070
BtsIMutI CAGTG 1 cut(s) 877
BtuMI TCGCGA 1 cut(s) 345
BveI ACCTGC 3 cut(s) 740, 1319, 1337
Cac8I GCNNGC 1 cut(s) 343
CciI TCATGA 1 cut(s) 1074
CfoI GCGC 5 cut(s) 394, 533, 592, 862, 1377
Cfr10I RCCGGY 6 cut(s) 470, 1003, 1009, 1102, 1135, 1304
Cfr13I GGNCC 1 cut(s) 824
CseI GACGC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 209, 939
CspAI ACCGGT 4 cut(s) 1009, 1102, 1135, 1304
CviAII CATG 9 cut(s) 25, 65, 317, 374, 684, 727, 982, 1075, 1255
CviQI GTAC 2 cut(s) 209, 939
DdeI CTNAG 3 cut(s) 395, 1267, 1287
DpnI GATC 3 cut(s) 90, 176, 282
DpnII GATC 3 cut(s) 88, 174, 280
DrdI GACNNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 1147
EciI GGCGGA 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 683
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 824
Eco91I GGTNACC 1 cut(s) 443
EcoO65I GGTNACC 1 cut(s) 443
EcoRII CCWGG 2 cut(s) 181, 826
EcoT14I CCWWGG 1 cut(s) 683
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 2 cut(s) 1032, 1076
FaeI CATG 9 cut(s) 28, 68, 320, 377, 687, 730, 985, 1078, 1258
FatI CATG 9 cut(s) 24, 64, 316, 373, 683, 726, 981, 1074, 1254
FauI CCCGC 1 cut(s) 1101
Fnu4HI GCNGC 6 cut(s) 593, 1097, 1124, 1326, 1341, 1344
Fsp4HI GCNGC 6 cut(s) 593, 1097, 1124, 1326, 1341, 1344
FspBI CTAG 4 cut(s) 702, 936, 1322, 1417
GlaI GCGC 5 cut(s) 393, 532, 591, 861, 1376
GluI GCNGC 6 cut(s) 593, 1097, 1124, 1326, 1341, 1344
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 1 cut(s) 134
HhaI GCGC 5 cut(s) 394, 533, 592, 862, 1377
Hin1II CATG 9 cut(s) 28, 68, 320, 377, 687, 730, 985, 1078, 1258
Hin6I GCGC 5 cut(s) 392, 531, 590, 860, 1375
HinP1I GCGC 5 cut(s) 392, 531, 590, 860, 1375
HinfI GANTC 5 cut(s) 28, 507, 768, 1018, 1111
HphI GGTGA 9 cut(s) 116, 196, 1027, 1053, 1120, 1149, 1150, 1231, 1253
Hpy166II GTNNAC 1 cut(s) 1244
Hpy188I TCNGA 5 cut(s) 331, 527, 760, 773, 844
Hpy188III TCNNGA 3 cut(s) 215, 344, 1075
Hpy8I GTNNAC 1 cut(s) 1244
Hpy99I CGWCG 5 cut(s) 266, 350, 467, 824, 1156
HpyAV CCTTC 6 cut(s) 497, 591, 650, 781, 1232, 1365
HpyCH4III ACNGT 5 cut(s) 85, 104, 208, 304, 551
HpyCH4IV ACGT 6 cut(s) 264, 275, 348, 353, 839, 1069
HpyCH4V TGCA 1 cut(s) 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 994, 1087, 1096, 1325
HpyF3I CTNAG 3 cut(s) 395, 1267, 1287
HpySE526I ACGT 6 cut(s) 264, 275, 348, 353, 839, 1069
Hsp92II CATG 9 cut(s) 28, 68, 320, 377, 687, 730, 985, 1078, 1258
HspAI GCGC 5 cut(s) 392, 531, 590, 860, 1375
Kzo9I GATC 3 cut(s) 88, 174, 280
LmnI GCTCC 3 cut(s) 19, 830, 1409
Lsp1109I GCAGC 3 cut(s) 604, 1337, 1355
LweI GCATC 1 cut(s) 1300
MaeI CTAG 4 cut(s) 702, 936, 1322, 1417
MaeII ACGT 6 cut(s) 264, 275, 348, 353, 839, 1069
MalI GATC 3 cut(s) 90, 176, 282
MbiI CCGCTC 1 cut(s) 1404
MboI GATC 3 cut(s) 88, 174, 280
MboII GAAGA 7 cut(s) 690, 703, 890, 984, 1050, 1077, 1269
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 6 cut(s) 133, 168, 270, 490, 782, 882
MlyI GAGTC 1 cut(s) 516
MmeI TCCRAC 1 cut(s) 354
MseI TTAA 3 cut(s) 236, 636, 929
MslI CAYNNNNRTG 2 cut(s) 83, 725
MspA1I CMGCKG 1 cut(s) 1147
MspR9I CCNGG 2 cut(s) 183, 828
MvaI CCWGG 2 cut(s) 183, 828
MvnI CGCG 2 cut(s) 345, 862
MwoI GCNNNNNNNGC 5 cut(s) 11, 994, 1087, 1096, 1325
NcoI CCATGG 1 cut(s) 683
NdeII GATC 3 cut(s) 88, 174, 280
NlaIII CATG 9 cut(s) 28, 68, 320, 377, 687, 730, 985, 1078, 1258
NlaIV GGNNCC 2 cut(s) 1008, 1041
NmuCI GTSAC 6 cut(s) 98, 163, 554, 895, 1138, 1155
NruI TCGCGA 1 cut(s) 345
PagI TCATGA 1 cut(s) 1074
PfeI GAWTC 4 cut(s) 28, 768, 1018, 1111
PfoI TCCNGGA 1 cut(s) 181
PinAI ACCGGT 4 cut(s) 1009, 1102, 1135, 1304
PkrI GCNGC 6 cut(s) 594, 1098, 1125, 1327, 1342, 1345
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Ppu21I YACGTR 1 cut(s) 276
Psp1406I AACGTT 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 181, 826
PspEI GGTNACC 1 cut(s) 443
PspGI CCWGG 2 cut(s) 181, 826
PspN4I GGNNCC 2 cut(s) 1008, 1041
PspPI GGNCC 1 cut(s) 824
PvuII CAGCTG 1 cut(s) 1147
RruI TCGCGA 1 cut(s) 345
RsaI GTAC 2 cut(s) 210, 940
RsaNI GTAC 2 cut(s) 209, 939
RseI CAYNNNNRTG 2 cut(s) 83, 725
SaqAI TTAA 3 cut(s) 236, 636, 929
SatI GCNGC 6 cut(s) 593, 1097, 1124, 1326, 1341, 1344
Sau3AI GATC 3 cut(s) 88, 174, 280
Sau96I GGNCC 1 cut(s) 824
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 1 cut(s) 516
ScrFI CCNGG 2 cut(s) 183, 828
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 1 cut(s) 1300
SfcI CTRYAG 1 cut(s) 1366
SgrAI CRCCGGYG 4 cut(s) 1009, 1102, 1135, 1304
SinI GGWCC 1 cut(s) 824
SmiMI CAYNNNNRTG 2 cut(s) 83, 725
Sse9I AATT 6 cut(s) 133, 168, 270, 490, 782, 882
SsiI CCGC 7 cut(s) 962, 1094, 1097, 1124, 1166, 1341, 1402
SspMI CTAG 4 cut(s) 702, 936, 1322, 1417
StyD4I CCNGG 2 cut(s) 181, 826
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 5 cut(s) 85, 104, 208, 304, 551
TaiI ACGT 6 cut(s) 267, 278, 351, 356, 842, 1072
TaqI TCGA 4 cut(s) 173, 434, 462, 644
TasI AATT 6 cut(s) 133, 168, 270, 490, 782, 882
TatI WGTACW 1 cut(s) 938
TauI GCSGC 3 cut(s) 1099, 1126, 1343
TfiI GAWTC 4 cut(s) 28, 768, 1018, 1111
Tru1I TTAA 3 cut(s) 236, 636, 929
Tru9I TTAA 3 cut(s) 236, 636, 929
TscAI CASTG 1 cut(s) 877
TseFI GTSAC 6 cut(s) 98, 163, 554, 895, 1138, 1155
TseI GCWGC 3 cut(s) 592, 1325, 1343
Tsp45I GTSAC 6 cut(s) 98, 163, 554, 895, 1138, 1155
TspDTI ATGAA 8 cut(s) 40, 217, 305, 325, 508, 998, 1064, 1091
TspGWI ACGGA 2 cut(s) 837, 1185
TspRI CASTG 1 cut(s) 877
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 1 cut(s) 882
XspI CTAG 4 cut(s) 702, 936, 1322, 1417
ZrmI AGTACT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.