MD15G1025000.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003654
Physical Location & Seq
Reverse (-)
1474489 .. 1476347
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1025000.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGTGGCTGTTTGTGGTACTTCTATGAAGACCAAGTGCTTCTTTGTCATGGCTTCATTGTATGTTCTTCTGGCGCTTTCTCAAGCAGAAGCAGAAACAACTCAGGGGTCCACGGCAATTCTAATAACCGTGGATCAGTCTGGGAAAGGCGATTACGAGAAAATACAAGATGCCATCGACGCGGTGCCGTCGAATAACGTCGATCTTGTGTTCATTAGGGTTAAGCCTGGAGTCTACAAAGAAAAAATTACTGTCCCCGCGGACAAACCCTTTATTACAATAAGTGGCACAAAGGCAGTTGACACTATAATTACGTGGAACGACAGCGGAGATATATTTGAATCTGCAACTTTCTCTGTGTTGGCTTCGGATTTTATAGGGCGCTACCTCACAATTCAGAACACTTATGGAAGCGGTGGTAAAGCAGTGGCGCTGAGGGCCTCAGGGGATAGAGCAGCATTTTATGTGTGCAGAATTTTGTCTTACCAAGATACCCTACTTGACGATACCGGCAGGCACTACTACAACAACTGTTATATTGAAGGAGCCACTGATTTTATATGTGGAAATGGTGCTTCTTTTTTTGAGAAGTGCCATTTGCATTCGATTTCGAATGGAGGAGGAGCCATAACAGCCCAAAGGAGGATGTCACCTTCAGAGGACACTGGGTTCTTTTTCTTTGGGTGCAAGATCACTGGCGTCAAAACGGCAGTGTTAGGAAGACCATGGGGTGTCTATTCCAGAGTCATTTTTGCCTTCACTTACATGCCTAATATCATACTCCCTCAGGGTTGGAACGACTGGGGTAACTCAGCCGACCAATTAAGTTCTGTTTACTATGGACAATACAAATGTTCTGGACCTGGAGCTGTGACATCAAAAAGAGTTGACTGGTCACGAGTCCTAACGAGTGAAGAAGCTGCACCTTTCATAACTAAGGACTCCATTGACGGCAAAGCTTGGATTAGGGCAGCGCCTACCAGTTTCAAGAAGAAGGCATTTCCCCCCAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

339

Amino Acids

36.86

Weight (kDa)

8.31

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 42 - 318 2.2e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 234
AccII CGCG 2 cut(s) 182, 260
AciI CCGC 5 cut(s) 182, 258, 260, 327, 414
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 474
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 699
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 1 cut(s) 642
AgsI TTSAA 3 cut(s) 341, 542, 988
AjnI CCWGG 2 cut(s) 226, 862
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 3 cut(s) 869, 920, 959
AluI AGCT 3 cut(s) 869, 920, 959
AlwI GGATC 1 cut(s) 141
AoxI GGCC 1 cut(s) 438
ApeKI GCWGC 3 cut(s) 455, 920, 971
ApoI RAATTY 1 cut(s) 474
AspLEI GCGC 4 cut(s) 76, 384, 433, 976
AspS9I GGNCC 3 cut(s) 108, 438, 860
AsuHPI GGTGA 1 cut(s) 642
AsuII TTCGAA 1 cut(s) 611
AvaII GGWCC 2 cut(s) 108, 860
AxyI CCTNAGG 2 cut(s) 442, 786
BanI GGYRCC 1 cut(s) 184
BauI CACGAG 1 cut(s) 897
BbsI GAAGAC 2 cut(s) 35, 727
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 3 cut(s) 467, 907, 983
BccI CCATC 1 cut(s) 182
BceAI ACGGC 4 cut(s) 129, 172, 723, 967
BciT130I CCWGG 2 cut(s) 228, 864
BfoI RGCGCY 4 cut(s) 77, 385, 434, 977
BisI GCNGC 3 cut(s) 456, 921, 972
BlsI GCNGC 3 cut(s) 457, 922, 973
Bme1390I CCNGG 2 cut(s) 228, 864
Bme18I GGWCC 2 cut(s) 108, 860
BmgT120I GGNCC 3 cut(s) 108, 438, 860
BmiI GGNNCC 4 cut(s) 109, 186, 547, 625
BmrFI CCNGG 2 cut(s) 228, 864
BmrI ACTGGG 2 cut(s) 675, 811
BmsI GCATC 1 cut(s) 160
BmuI ACTGGG 2 cut(s) 675, 811
BpiI GAAGAC 2 cut(s) 35, 727
BpmI CTGGAG 2 cut(s) 249, 885
Bpu10I CCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 111, 129, 258, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 642
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 5 cut(s) 670, 700, 806, 896, 981
Bse21I CCTNAGG 2 cut(s) 442, 786
BseBI CCWGG 2 cut(s) 228, 864
BseDI CCNNGG 4 cut(s) 111, 129, 258, 725
BseGI GGATG 1 cut(s) 651
BseLI CCNNNNNNNGG 1 cut(s) 642
BseMII CTCAG 5 cut(s) 116, 425, 456, 800, 825
BseNI ACTGG 5 cut(s) 670, 700, 806, 896, 981
BseRI GAGGAG 2 cut(s) 633, 636
BseXI GCAGC 3 cut(s) 467, 907, 983
BsgI GTGCAG 2 cut(s) 490, 906
Bsh1236I CGCG 2 cut(s) 182, 260
BshFI GGCC 1 cut(s) 440
BshNI GGYRCC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 642
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 1 cut(s) 440
Bsp119I TTCGAA 1 cut(s) 611
Bsp143I GATC 3 cut(s) 133, 202, 690
Bsp19I CCATGG 1 cut(s) 725
BspACI CCGC 5 cut(s) 182, 258, 260, 327, 414
BspANI GGCC 1 cut(s) 440
BspCNI CTCAG 5 cut(s) 115, 426, 455, 799, 824
BspFNI CGCG 2 cut(s) 182, 260
BspLI GGNNCC 4 cut(s) 109, 186, 547, 625
BspPI GGATC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 611
BspT107I GGYRCC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 5 cut(s) 670, 700, 806, 896, 981
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 4 cut(s) 111, 129, 258, 725
BssMI GATC 3 cut(s) 133, 202, 690
BssNI GRCGYC 1 cut(s) 699
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 1 cut(s) 725
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 2 cut(s) 228, 864
Bst4CI ACNGT 3 cut(s) 130, 253, 533
BstACI GRCGYC 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 6 cut(s) 102, 434, 442, 786, 811, 936
BstDSI CCRYGG 4 cut(s) 111, 129, 258, 725
BstF5I GGATG 1 cut(s) 651
BstFNI CGCG 2 cut(s) 182, 260
BstH2I RGCGCY 4 cut(s) 77, 385, 434, 977
BstHHI GCGC 4 cut(s) 76, 384, 433, 976
BstKTI GATC 3 cut(s) 136, 205, 693
BstMBI GATC 3 cut(s) 133, 202, 690
BstMWI GCNNNNNNNGC 3 cut(s) 179, 437, 632
BstNI CCWGG 2 cut(s) 228, 864
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 2 cut(s) 226, 862
BstUI CGCG 2 cut(s) 182, 260
BstV1I GCAGC 3 cut(s) 467, 907, 983
BstV2I GAAGAC 2 cut(s) 35, 727
Bsu36I CCTNAGG 2 cut(s) 442, 786
BsuRI GGCC 1 cut(s) 440
BtgI CCRYGG 4 cut(s) 111, 129, 258, 725
BtsCI GGATG 1 cut(s) 651
BtsI GCAGTG 2 cut(s) 432, 717
BtsIMutI CAGTG 5 cut(s) 432, 549, 663, 693, 717
Cac8I GCNNGC 1 cut(s) 515
CfoI GCGC 4 cut(s) 76, 384, 433, 976
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 3 cut(s) 108, 438, 860
Cfr42I CCGCGG 1 cut(s) 261
CseI GACGC 2 cut(s) 188, 688
Csp6I GTAC 1 cut(s) 18
CviAII CATG 3 cut(s) 49, 726, 766
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 6 cut(s) 102, 434, 442, 786, 811, 936
DpnI GATC 3 cut(s) 135, 204, 692
DpnII GATC 3 cut(s) 133, 202, 690
Eco130I CCWWGG 1 cut(s) 725
Eco47I GGWCC 2 cut(s) 108, 860
Eco57I CTGAAG 1 cut(s) 639
Eco81I CCTNAGG 2 cut(s) 442, 786
EcoO109I RGGNCCY 1 cut(s) 438
EcoRII CCWGG 2 cut(s) 226, 862
EcoT14I CCWWGG 1 cut(s) 725
ErhI CCWWGG 1 cut(s) 725
FaeI CATG 3 cut(s) 52, 729, 769
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 239
FatI CATG 3 cut(s) 48, 725, 765
FauI CCCGC 1 cut(s) 265
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 456, 921, 972
FokI GGATG 1 cut(s) 658
Fsp4HI GCNGC 3 cut(s) 456, 921, 972
GlaI GCGC 4 cut(s) 75, 383, 432, 975
GluI GCNGC 3 cut(s) 456, 921, 972
GsuI CTGGAG 2 cut(s) 249, 885
HaeII RGCGCY 4 cut(s) 77, 385, 434, 977
HaeIII GGCC 1 cut(s) 440
HapII CCGG 1 cut(s) 510
HgaI GACGC 2 cut(s) 188, 688
HhaI GCGC 4 cut(s) 76, 384, 433, 976
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 3 cut(s) 52, 729, 769
Hin6I GCGC 4 cut(s) 74, 382, 431, 974
HinP1I GCGC 4 cut(s) 74, 382, 431, 974
HincII GTYRAC 2 cut(s) 301, 889
HindII GTYRAC 2 cut(s) 301, 889
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 5 cut(s) 231, 341, 744, 900, 941
HpaII CCGG 1 cut(s) 510
HphI GGTGA 1 cut(s) 642
Hpy166II GTNNAC 5 cut(s) 111, 235, 301, 835, 889
Hpy188I TCNGA 3 cut(s) 370, 399, 658
Hpy188III TCNNGA 4 cut(s) 741, 858, 897, 988
Hpy8I GTNNAC 5 cut(s) 111, 235, 301, 835, 889
Hpy99I CGWCG 3 cut(s) 182, 193, 203
HpyAV CCTTC 4 cut(s) 536, 663, 766, 988
HpyCH4III ACNGT 3 cut(s) 130, 253, 533
HpyCH4IV ACGT 2 cut(s) 198, 314
HpyCH4V TGCA 5 cut(s) 347, 471, 601, 687, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 437, 632
HpyF3I CTNAG 6 cut(s) 102, 434, 442, 786, 811, 936
HpySE526I ACGT 2 cut(s) 198, 314
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 3 cut(s) 52, 729, 769
HspAI GCGC 4 cut(s) 74, 382, 431, 974
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 3 cut(s) 133, 202, 690
LmnI GCTCC 3 cut(s) 545, 623, 866
Lsp1109I GCAGC 3 cut(s) 467, 907, 983
LweI GCATC 1 cut(s) 160
MaeII ACGT 2 cut(s) 198, 314
MaeIII GTNAC 4 cut(s) 648, 806, 871, 894
MalI GATC 3 cut(s) 135, 204, 692
MboI GATC 3 cut(s) 133, 202, 690
MboII GAAGA 5 cut(s) 40, 59, 732, 926, 1003
MluCI AATT 7 cut(s) 117, 246, 309, 393, 474, 821, 1012
MlyI GAGTC 4 cut(s) 240, 753, 909, 935
MmeI TCCRAC 1 cut(s) 773
MnlI CCTC 8 cut(s) 398, 429, 451, 611, 614, 636, 652, 795
MseI TTAA 2 cut(s) 222, 824
MslI CAYNNNNRTG 1 cut(s) 764
MspA1I CMGCKG 2 cut(s) 260, 327
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 2 cut(s) 228, 864
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 2 cut(s) 228, 864
MvnI CGCG 2 cut(s) 182, 260
MwoI GCNNNNNNNGC 3 cut(s) 179, 437, 632
NcoI CCATGG 1 cut(s) 725
NdeII GATC 3 cut(s) 133, 202, 690
NlaIII CATG 3 cut(s) 52, 729, 769
NlaIV GGNNCC 4 cut(s) 109, 186, 547, 625
NmuCI GTSAC 3 cut(s) 648, 871, 894
NspI RCATGY 1 cut(s) 769
NspV TTCGAA 1 cut(s) 611
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 3 cut(s) 457, 922, 973
PleI GAGTC 4 cut(s) 239, 752, 908, 935
PpsI GAGTC 4 cut(s) 239, 752, 908, 935
Ppu21I YACGTR 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 226, 862
PspGI CCWGG 2 cut(s) 226, 862
PspN4I GGNNCC 4 cut(s) 109, 186, 547, 625
PspPI GGNCC 3 cut(s) 108, 438, 860
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
RseI CAYNNNNRTG 1 cut(s) 764
SacII CCGCGG 1 cut(s) 261
SaqAI TTAA 2 cut(s) 222, 824
SatI GCNGC 3 cut(s) 456, 921, 972
Sau3AI GATC 3 cut(s) 133, 202, 690
Sau96I GGNCC 3 cut(s) 108, 438, 860
SchI GAGTC 4 cut(s) 240, 753, 909, 935
ScrFI CCNGG 2 cut(s) 228, 864
SetI ASST 9 cut(s) 201, 317, 390, 655, 865, 871, 922, 928, 961
SfaNI GCATC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 611
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 2 cut(s) 108, 860
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 7 cut(s) 117, 246, 309, 393, 474, 821, 1012
SsiI CCGC 5 cut(s) 182, 258, 260, 327, 414
StyD4I CCNGG 2 cut(s) 226, 862
StyI CCWWGG 1 cut(s) 725
TaaI ACNGT 3 cut(s) 130, 253, 533
TaiI ACGT 2 cut(s) 201, 317
TaqI TCGA 5 cut(s) 177, 191, 201, 605, 611
TasI AATT 7 cut(s) 117, 246, 309, 393, 474, 821, 1012
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 2 cut(s) 222, 824
Tru9I TTAA 2 cut(s) 222, 824
TscAI CASTG 5 cut(s) 432, 556, 670, 700, 717
TseFI GTSAC 3 cut(s) 648, 871, 894
TseI GCWGC 3 cut(s) 455, 920, 971
Tsp45I GTSAC 3 cut(s) 648, 871, 894
TspDTI ATGAA 4 cut(s) 41, 45, 202, 919
TspRI CASTG 5 cut(s) 432, 556, 670, 700, 717
VpaK11BI GGWCC 2 cut(s) 108, 860
XapI RAATTY 1 cut(s) 474
XceI RCATGY 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.