Rh6DG413800

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003654
Physical Location & Seq
Reverse (-)
59870630 .. 59872369
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG413800.1

Sequence Viewer

Length: 1740 bp
ATGGCGCGTAGGGCTCTAGTTAGAATGATTGTCATAATTTTGTATGCTTTCGTCACTATTTCTCAAGCGGAAAATATAATAACAGTTGATCAATCCGGTAACGGTCATTACGGTAAAATACAAGATGCTATTGATTCTGTTCCATCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGAACTTACAAAGAAAGTATTACCATACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAGAAGCAAGTGATGTGATGATAACGTGGGATCAGAGAGGGGACATTGATAAAGTTGCAACATTTCATGTTTCGGCTTCCGACTTTGTAGCGCGCTACATAACAATTCAAAATACATTTGGGCCTGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAATAGCGTTTTATGACTGCTCAATTTTAAGTTATCAGGATACTCTATTTGACGAAATAGGCAGACACTACTACGGAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCGCAACGAAGAGAAGCGTCAACAGATATTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAAAGGCGAAAAAACATTTTTAGGAAGACCATGGGTGGGATCCTATGCTAGGGTCATATTTGCCTACACTAACATGCAAGATGTGATAGAACCACTAGGTTGGTCTCGTTATTCTGATGGATCCACAGAACAAGAATTAATGACAAACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAAAATTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAGGATTGGATAAAGTCTACGCCGGTAACAGCGTCGCCTCCACCTAAAAAGGAGACGCCGTCATTGCCTCCATCGGTACTGGATACACCGCCACCCTCACCACCACCTCCCATGAAGCCGTCTGCACCACCACCAGCACCGGTCATCCCGGCGCCTGTAATCCCGTCTCCACCACCACAAGCCCCGGTGGTACCAACTTCCCCACCACCGGTGATTCCATTAGTTTCTCCACCAGCACCGGCAGTGATTCCATCGTCGCCACCACTAGCACCCGTGGTGATTTTATCTCCGCCAGCACCAGCACCCGTGATCCCGTCGTCGCCACCACCGACAACGGCACCACCAGTGATTCCGTTGTTGCCCCCACCGGCACCGGTGGTGATTCCATCGTCGCCCCCACCAGCACCGGTGGCGATCCCGTCGTCGCCACCATCGACACCTGTGACCCCGTGGTTCCCGTGGTTCGCACCACCGGCACCGGTGATACCGACGTTGCCACCACTGGTAAATCCATTTTCACCACCACCGGTACCGATGATTCCGTTGTTGCCCCCACTGGCACCGGTGGTGATCTTCTCACCAGCGACACCCGTGATCTCGTGGTTCGCACCACCGCCACCATCACCACGAGAGAGGGTGATTATAACTCCGTGGTCGCCTCCACCAAGATACATGGACGGCAGAGCTCAAGAAGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGCATCCTCTTCAATCTTTAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTAACCTCGGCAGTTTCACTATACTTGTTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

579

Amino Acids

62.08

Weight (kDa)

6.99

Isoelectric Point (pI)

66.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 308 1.5e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
Acc65I GGTACC 2 cut(s) 1114, 1453
AccB1I GGYRCC 7 cut(s) 1075, 1114, 1261, 1294, 1399, 1453, 1483
AccI GTMKAC 1 cut(s) 941
AccII CGCG 3 cut(s) 7, 343, 868
AciI CCGC 5 cut(s) 68, 1013, 1214, 1538, 1686
AclWI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1228, 1333
AcsI RAATTY 2 cut(s) 872, 895
AcyI GRCGYC 2 cut(s) 980, 1076
AfaI GTAC 4 cut(s) 244, 1002, 1116, 1455
AfiI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
AgeI ACCGGT 7 cut(s) 1063, 1132, 1297, 1330, 1402, 1450, 1486
AgsI TTSAA 5 cut(s) 359, 503, 548, 820, 1665
AjnI CCWGG 2 cut(s) 181, 373
AluBI AGCT 1 cut(s) 1610
AluI AGCT 1 cut(s) 1610
Alw21I GWGCWC 1 cut(s) 1612
Alw26I GTCTC 4 cut(s) 406, 762, 971, 1095
AlwI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1228, 1333
Ama87I CYCGRG 1 cut(s) 1648
AoxI GGCC 2 cut(s) 371, 1734
ApoI RAATTY 2 cut(s) 872, 895
AseI ATTAAT 1 cut(s) 791
AsiGI ACCGGT 7 cut(s) 1063, 1132, 1297, 1330, 1402, 1450, 1486
Asp700I GAANNNNTTC 1 cut(s) 1679
Asp718I GGTACC 2 cut(s) 1114, 1453
AspLEI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1078
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 1073, 1109
AvaI CYCGRG 1 cut(s) 1648
BaeI ACNNNNGTAYC 2 cut(s) 1400, 1433
BamHI GGATCC 3 cut(s) 157, 692, 773
BanI GGYRCC 7 cut(s) 1075, 1114, 1261, 1294, 1399, 1453, 1483
BanII GRGCYC 2 cut(s) 16, 1612
BauI CACGAG 2 cut(s) 1522, 1551
BbsI GAAGAC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 1612
BccI CCATC 7 cut(s) 151, 764, 1003, 1183, 1318, 1363, 1552
BceAI ACGGC 4 cut(s) 967, 1027, 1275, 1618
BciT130I CCWGG 2 cut(s) 183, 375
BciVI GTATCC 2 cut(s) 445, 1000
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 1073, 1109
BcoDI GTCTC 4 cut(s) 406, 762, 971, 1095
BfaI CTAG 5 cut(s) 17, 702, 749, 833, 1190
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 2 cut(s) 534, 1079
BfuI GTATCC 2 cut(s) 445, 1000
BisI GCNGC 1 cut(s) 1687
BlsI GCNGC 1 cut(s) 1688
Bme1390I CCNGG 4 cut(s) 183, 375, 1073, 1109
BmeT110I CYCGRG 1 cut(s) 1648
BmgT120I GGNCC 1 cut(s) 371
BmrFI CCNGG 4 cut(s) 183, 375, 1073, 1109
BmsI GCATC 2 cut(s) 115, 1664
BpiI GAAGAC 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 48, 1596
BpuMI CCSGG 2 cut(s) 1073, 1109
BsaHI GRCGYC 2 cut(s) 980, 1076
BsaI GGTCTC 2 cut(s) 406, 762
BsaJI CCNNGG 8 cut(s) 683, 1107, 1197, 1373, 1382, 1574, 1647, 1710
BsaWI WCCGGW 8 cut(s) 95, 1063, 1132, 1297, 1330, 1402, 1450, 1486
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 612, 642, 968, 998
Bsc4I CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
Bse1I ACTGG 6 cut(s) 534, 655, 1008, 1268, 1431, 1485
Bse3DI GCAATG 1 cut(s) 986
BseBI CCWGG 2 cut(s) 183, 375
BseDI CCNNGG 8 cut(s) 683, 1107, 1197, 1373, 1382, 1574, 1647, 1710
BseGI GGATG 3 cut(s) 845, 1068, 1655
BseLI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
BseMI GCAATG 1 cut(s) 986
BseNI ACTGG 6 cut(s) 534, 655, 1008, 1268, 1431, 1485
BsePI GCGCGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 3 cut(s) 7, 343, 868
BshFI GGCC 2 cut(s) 373, 1736
BshNI GGYRCC 7 cut(s) 1075, 1114, 1261, 1294, 1399, 1453, 1483
BshTI ACCGGT 7 cut(s) 1063, 1132, 1297, 1330, 1402, 1450, 1486
BsiHKAI GWGCWC 1 cut(s) 1612
BsiHKCI CYCGRG 1 cut(s) 1648
BslFI GGGAC 2 cut(s) 305, 1708
BslI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
BsmAI GTCTC 4 cut(s) 406, 762, 971, 1095
BsmBI CGTCTC 2 cut(s) 971, 1095
BsmFI GGGAC 2 cut(s) 305, 1708
BsnI GGCC 2 cut(s) 373, 1736
Bso31I GGTCTC 2 cut(s) 406, 762
BsoBI CYCGRG 1 cut(s) 1648
Bsp1286I GDGCHC 2 cut(s) 16, 1612
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 5 cut(s) 68, 1013, 1214, 1538, 1686
BspANI GGCC 2 cut(s) 373, 1736
BspFNI CGCG 3 cut(s) 7, 343, 868
BspPI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1228, 1333
BspT107I GGYRCC 7 cut(s) 1075, 1114, 1261, 1294, 1399, 1453, 1483
BspTNI GGTCTC 2 cut(s) 406, 762
BsrDI GCAATG 1 cut(s) 986
BsrI ACTGG 6 cut(s) 534, 655, 1008, 1268, 1431, 1485
BssECI CCNNGG 8 cut(s) 683, 1107, 1197, 1373, 1382, 1574, 1647, 1710
BssHII GCGCGC 1 cut(s) 341
BssNI GRCGYC 2 cut(s) 980, 1076
BssSI CACGAG 2 cut(s) 1522, 1551
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 2 cut(s) 1522, 1551
Bst2UI CCWGG 2 cut(s) 183, 375
Bst4CI ACNGT 3 cut(s) 85, 104, 113
Bst6I CTCTTC 2 cut(s) 592, 1666
BstACI GRCGYC 2 cut(s) 980, 1076
BstC8I GCNNGC 3 cut(s) 343, 1218, 1701
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 5 cut(s) 683, 1197, 1373, 1382, 1574
BstENI CCTNNNNNAGG 2 cut(s) 573, 700
BstF5I GGATG 3 cut(s) 845, 1068, 1655
BstFNI CGCG 3 cut(s) 7, 343, 868
BstH2I RGCGCY 2 cut(s) 534, 1079
BstHHI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1078
BstMAI GTCTC 4 cut(s) 406, 762, 971, 1095
BstMWI GCNNNNNNNGC 4 cut(s) 11, 988, 1334, 1397
BstNI CCWGG 2 cut(s) 183, 375
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 4 cut(s) 181, 373, 1071, 1107
BstSFI CTRYAG 2 cut(s) 249, 570
BstUI CGCG 3 cut(s) 7, 343, 868
BstV2I GAAGAC 1 cut(s) 685
BstX2I RGATCY 3 cut(s) 157, 692, 773
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 3 cut(s) 157, 692, 773
BsuI GTATCC 2 cut(s) 445, 1000
BsuRI GGCC 2 cut(s) 373, 1736
BtgI CCRYGG 5 cut(s) 683, 1197, 1373, 1382, 1574
BtsCI GGATG 3 cut(s) 845, 1068, 1655
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 5 cut(s) 883, 1173, 1275, 1424, 1478
Cac8I GCNNGC 3 cut(s) 343, 1218, 1701
CfoI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1078
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 4 cut(s) 594, 945, 988, 1705
Csp6I GTAC 4 cut(s) 243, 1001, 1115, 1454
CspAI ACCGGT 7 cut(s) 1063, 1132, 1297, 1330, 1402, 1450, 1486
CviAII CATG 6 cut(s) 317, 684, 727, 1036, 1597, 1633
CviQI GTAC 4 cut(s) 243, 1001, 1115, 1454
DdeI CTNAG 1 cut(s) 395
DinI GGCGCC 1 cut(s) 1077
Eam1104I CTCTTC 2 cut(s) 592, 1666
EarI CTCTTC 2 cut(s) 592, 1666
EciI GGCGGA 1 cut(s) 1203
Ecl136II GAGCTC 1 cut(s) 1610
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 16, 1612
Eco31I GGTCTC 2 cut(s) 406, 762
Eco53kI GAGCTC 1 cut(s) 1610
Eco88I CYCGRG 1 cut(s) 1648
EcoICRI GAGCTC 1 cut(s) 1610
EcoNI CCTNNNNNAGG 2 cut(s) 573, 700
EcoRII CCWGG 2 cut(s) 181, 373
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 16, 1612
EgeI GGCGCC 1 cut(s) 1077
EheI GGCGCC 1 cut(s) 1077
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 2 cut(s) 971, 1095
FaeI CATG 6 cut(s) 320, 687, 730, 1039, 1600, 1636
FaqI GGGAC 2 cut(s) 305, 1708
FatI CATG 6 cut(s) 316, 683, 726, 1035, 1596, 1632
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 941
Fnu4HI GCNGC 1 cut(s) 1687
FokI GGATG 3 cut(s) 832, 1055, 1642
FriOI GRGCYC 2 cut(s) 16, 1612
Fsp4HI GCNGC 1 cut(s) 1687
FspBI CTAG 5 cut(s) 17, 702, 749, 833, 1190
GlaI GCGC 8 cut(s) 6, 342, 344, 393, 532, 591, 867, 1077
GluI GCNGC 1 cut(s) 1687
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 2 cut(s) 534, 1079
HaeIII GGCC 2 cut(s) 373, 1736
HgaI GACGC 4 cut(s) 594, 945, 988, 1705
HhaI GCGC 8 cut(s) 7, 343, 345, 394, 533, 592, 868, 1078
Hin1I GRCGYC 2 cut(s) 980, 1076
Hin1II CATG 6 cut(s) 320, 687, 730, 1039, 1600, 1636
Hin6I GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1076
HinP1I GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1076
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 6 cut(s) 134, 1138, 1171, 1273, 1306, 1462
Hpy166II GTNNAC 4 cut(s) 609, 812, 942, 1622
Hpy188I TCNGA 3 cut(s) 285, 331, 769
Hpy188III TCNNGA 3 cut(s) 215, 449, 1613
Hpy8I GTNNAC 4 cut(s) 609, 812, 942, 1622
Hpy99I CGWCG 9 cut(s) 830, 961, 1183, 1243, 1246, 1318, 1348, 1351, 1417
HpyAV CCTTC 2 cut(s) 497, 1610
HpyCH4III ACNGT 3 cut(s) 85, 104, 113
HpyCH4IV ACGT 2 cut(s) 275, 1415
HpyCH4V TGCA 4 cut(s) 308, 730, 893, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 988, 1334, 1397
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 275, 1415
Hsp92I GRCGYC 2 cut(s) 980, 1076
Hsp92II CATG 6 cut(s) 320, 687, 730, 1039, 1600, 1636
HspAI GCGC 8 cut(s) 5, 341, 343, 392, 531, 590, 866, 1076
KasI GGCGCC 1 cut(s) 1075
KpnI GGTACC 2 cut(s) 1118, 1457
Ksp22I TGATCA 1 cut(s) 88
LmnI GCTCC 1 cut(s) 836
LweI GCATC 2 cut(s) 115, 1664
MaeI CTAG 5 cut(s) 17, 702, 749, 833, 1190
MaeII ACGT 2 cut(s) 275, 1415
MaeIII GTNAC 6 cut(s) 52, 98, 494, 554, 949, 1366
MboII GAAGA 4 cut(s) 609, 690, 1489, 1653
MflI RGATCY 3 cut(s) 157, 692, 773
MhlI GDGCHC 2 cut(s) 16, 1612
Mly113I GGCGCC 1 cut(s) 1076
MmeI TCCRAC 2 cut(s) 354, 873
MroXI GAANNNNTTC 1 cut(s) 1679
MseI TTAA 6 cut(s) 236, 440, 636, 791, 858, 1671
MslI CAYNNNNRTG 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 1686
MspR9I CCNGG 4 cut(s) 183, 375, 1073, 1109
MvaI CCWGG 2 cut(s) 183, 375
MvnI CGCG 3 cut(s) 7, 343, 868
MwoI GCNNNNNNNGC 4 cut(s) 11, 988, 1334, 1397
NarI GGCGCC 1 cut(s) 1076
NciI CCSGG 2 cut(s) 1073, 1109
NcoI CCATGG 1 cut(s) 683
NlaIII CATG 6 cut(s) 320, 687, 730, 1039, 1600, 1636
NmeAIII GCCGAG 1 cut(s) 1691
NmuCI GTSAC 3 cut(s) 52, 554, 1366
NspI RCATGY 1 cut(s) 730
PauI GCGCGC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 1250, 1355
PdmI GAANNNNTTC 1 cut(s) 1679
PfeI GAWTC 6 cut(s) 134, 1138, 1171, 1273, 1306, 1462
PflFI GACNNNGTC 1 cut(s) 982
PinAI ACCGGT 7 cut(s) 1063, 1132, 1297, 1330, 1402, 1450, 1486
PkrI GCNGC 1 cut(s) 1688
PluTI GGCGCC 1 cut(s) 1079
PshBI ATTAAT 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1568
Psp124BI GAGCTC 1 cut(s) 1612
Psp6I CCWGG 2 cut(s) 181, 373
PspGI CCWGG 2 cut(s) 181, 373
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 3 cut(s) 157, 692, 773
PsyI GACNNNGTC 1 cut(s) 982
PteI GCGCGC 1 cut(s) 341
RsaI GTAC 4 cut(s) 244, 1002, 1116, 1455
RsaNI GTAC 4 cut(s) 243, 1001, 1115, 1454
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 1 cut(s) 1612
SaqAI TTAA 6 cut(s) 236, 440, 636, 791, 858, 1671
SatI GCNGC 1 cut(s) 1687
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 4 cut(s) 183, 375, 1073, 1109
SduI GDGCHC 2 cut(s) 16, 1612
SfaNI GCATC 2 cut(s) 115, 1664
SfcI CTRYAG 2 cut(s) 249, 570
SfoI GGCGCC 1 cut(s) 1077
SgrAI CRCCGGYG 5 cut(s) 1132, 1297, 1330, 1402, 1486
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 2 cut(s) 63, 1611
SmoI CTYRAG 2 cut(s) 63, 1611
SsiI CCGC 5 cut(s) 68, 1013, 1214, 1538, 1686
SspDI GGCGCC 1 cut(s) 1075
SspMI CTAG 5 cut(s) 17, 702, 749, 833, 1190
SstI GAGCTC 1 cut(s) 1612
StyD4I CCNGG 4 cut(s) 181, 373, 1071, 1107
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 3 cut(s) 85, 104, 113
TaiI ACGT 2 cut(s) 278, 1418
TaqI TCGA 2 cut(s) 173, 1358
TauI GCSGC 1 cut(s) 1689
TfiI GAWTC 6 cut(s) 134, 1138, 1171, 1273, 1306, 1462
Tru1I TTAA 6 cut(s) 236, 440, 636, 791, 858, 1671
Tru9I TTAA 6 cut(s) 236, 440, 636, 791, 858, 1671
TscAI CASTG 5 cut(s) 883, 1173, 1275, 1431, 1485
TseFI GTSAC 3 cut(s) 52, 554, 1366
Tsp45I GTSAC 3 cut(s) 52, 554, 1366
TspDTI ATGAA 3 cut(s) 217, 305, 1052
TspGWI ACGGA 5 cut(s) 501, 843, 1266, 1455, 1563
TspRI CASTG 5 cut(s) 883, 1173, 1275, 1431, 1485
Tth111I GACNNNGTC 1 cut(s) 982
VspI ATTAAT 1 cut(s) 791
XagI CCTNNNNNAGG 2 cut(s) 573, 700
XapI RAATTY 2 cut(s) 872, 895
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 1679
XspI CTAG 5 cut(s) 17, 702, 749, 833, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.