MD13G1062700.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003654
Physical Location & Seq
Reverse (-)
4365408 .. 4366445
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1062700.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCCGACGGTGTTACTTCTACGAAGTTCTTTCTCATTGCTCTTTTACTCTATACTGTCACCGCCGTTACCTCTCAAGTTGCAACCACAAATAATTATGCGTCTGACGAAGCCATTCTCATAACCGTGGACCAGTCCGGCAAAGGAGATTACTGGAAAATACAGGACGCCATTGATGCCGTGCCGTCGAATAATGCGGAGAATGTGTACATTGCAGTTAAACCTGGAACATACACTGAAAAAATTTGGGTGCCTGCAGATAAGCCATTTATTACGATAGCCGGCGCGGGGTCGGGCGAGACGATAATAACATGGAGTCAGGGCGGGGAGATATACGACACTCCAACCTTCACCGTCCTGGCTTCTGATTTTACAGGACGAACCCTCACAATTCAGAACACGTACGGGAAAGGAGCTCGAGCGGTGGCACTTAGGGCTTCCGGAGATAGGGCAGCATTCTACGATTGCAGTATCAAGTCGTATCAAGATACCCTACTTTCCGAAAACGGCAAGCAGTACTACAGAAACTGCTACATCGAAGGAGCCGTCGACTTCATATTCGGAAGCTCAGCCGCCCTTTTCCAGAACTGCCACTTGCACTCGACTGCGGATGAGGTCGGATTCATAACCGCCCACCGGAGGGACAACGCTTCAGAGAACTTGGGCTTCAGTTTCGTGGGGTGCAAGATAAGCGGCTTATCAAAGACAACCCTAGGACGGCCATGGAGCCCCTATTCCAGGGTTATCTTTGCCCTTACGCACATGTCGAATGTCGTTGCGCCGCAAGGTTGGGACGACTGGGGTCGGTCGCCCGACGAACTGAGTACTGTCTACTTCGGACAGTACAGATGTTGGGGAGCGGGAGCCGATACGTCGCAGAGAGTTAAGTGGGCCCGGGAACTTACGTTCCAAGAGGCTGCGCCGCTTATGACGAGTGGCTTCATTGGTGGCACATACTGGTTAAGGCAAGCTCCGACCAGGTTCAAGAGTATAAGTGCACTCCGTTCTTCAAATGTCACGGCTGCTGGAAATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

346

Amino Acids

37.64

Weight (kDa)

6.11

Isoelectric Point (pI)

26.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 41 - 315 3.6e-73 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AccBSI CCGCTC 2 cut(s) 422, 860
AccI GTMKAC 2 cut(s) 549, 831
AccII CGCG 1 cut(s) 287
AccIII TCCGGA 1 cut(s) 440
AcoI YGGCCR 2 cut(s) 3, 719
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 2 cut(s) 636, 652
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 5 cut(s) 209, 404, 518, 826, 845
AfiI CCNNNNNNNGG 7 cut(s) 288, 447, 636, 639, 640, 717, 738
AflIII ACRYGT 2 cut(s) 399, 762
AgsI TTSAA 2 cut(s) 985, 1011
AjnI CCWGG 4 cut(s) 223, 357, 737, 977
AloI GAACNNNNNNTCC 4 cut(s) 890, 891, 922, 923
AluBI AGCT 3 cut(s) 416, 567, 971
AluI AGCT 3 cut(s) 416, 567, 971
Alw21I GWGCWC 2 cut(s) 418, 1000
Alw26I GTCTC 1 cut(s) 293
Alw44I GTGCAC 1 cut(s) 996
AlwNI CAGNNNCTG 1 cut(s) 528
Ama87I CYCGRG 2 cut(s) 417, 894
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 3 cut(s) 3, 719, 891
ApaI GGGCCC 1 cut(s) 895
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 452, 917, 1022
ApoI RAATTY 1 cut(s) 243
ArsI GACNNNNNNTTYG 4 cut(s) 531, 542, 563, 574
Asp700I GAANNNNTTC 1 cut(s) 114
AspA2I CCTAGG 1 cut(s) 712
AspLEI GCGC 3 cut(s) 287, 781, 922
AspS9I GGNCC 3 cut(s) 130, 891, 892
AsuC2I CCSGG 2 cut(s) 895, 896
AsuHPI GGTGA 2 cut(s) 52, 343
AvaI CYCGRG 2 cut(s) 417, 894
AvaII GGWCC 1 cut(s) 130
AvrII CCTAGG 1 cut(s) 712
BaeGI GKGCMC 2 cut(s) 895, 1000
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 3 cut(s) 418, 731, 895
Bbv12I GWGCWC 2 cut(s) 418, 1000
BbvI GCAGC 3 cut(s) 464, 904, 1009
BceAI ACGGC 7 cut(s) 50, 164, 169, 523, 530, 734, 1035
BciT130I CCWGG 4 cut(s) 225, 359, 739, 979
BcnI CCSGG 2 cut(s) 895, 896
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 2 cut(s) 255, 520
BisI GCNGC 7 cut(s) 453, 573, 694, 782, 918, 923, 1023
BlnI CCTAGG 1 cut(s) 712
BlpI GCTNAGC 1 cut(s) 568
BlsI GCNGC 7 cut(s) 454, 574, 695, 783, 919, 924, 1024
BmcAI AGTACT 2 cut(s) 518, 826
Bme1390I CCNGG 6 cut(s) 225, 359, 739, 895, 896, 979
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 417, 894
BmgT120I GGNCC 3 cut(s) 130, 891, 892
BmiI GGNNCC 5 cut(s) 252, 544, 728, 865, 893
BmrFI CCNGG 6 cut(s) 225, 359, 739, 895, 896, 979
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 1 cut(s) 166
BmuI ACTGGG 1 cut(s) 808
BoxI GACNNNNGTC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 568
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 895, 896
BsaAI YACGTR 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 5 cut(s) 126, 712, 722, 738, 894
BsaWI WCCGGW 2 cut(s) 440, 636
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 7 cut(s) 288, 447, 636, 639, 640, 717, 738
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 4 cut(s) 133, 158, 803, 962
Bse3DI GCAATG 2 cut(s) 36, 210
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 4 cut(s) 225, 359, 739, 979
BseDI CCNNGG 5 cut(s) 126, 712, 722, 738, 894
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 7 cut(s) 288, 447, 636, 639, 640, 717, 738
BseMI GCAATG 2 cut(s) 36, 210
BseMII CTCAG 2 cut(s) 582, 812
BseNI ACTGG 4 cut(s) 133, 158, 803, 962
BseSI GKGCMC 2 cut(s) 895, 1000
BseXI GCAGC 3 cut(s) 464, 904, 1009
Bsh1236I CGCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 809
BshFI GGCC 3 cut(s) 5, 721, 893
BshNI GGYRCC 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 809
BsiHKAI GWGCWC 2 cut(s) 418, 1000
BsiHKCI CYCGRG 2 cut(s) 417, 894
BsiSI CCGG 5 cut(s) 138, 282, 441, 637, 895
BsiWI CGTACG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 656, 806
BslI CCNNNNNNNGG 7 cut(s) 288, 447, 636, 639, 640, 717, 738
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 2 cut(s) 656, 806
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 3 cut(s) 5, 721, 893
BsoBI CYCGRG 2 cut(s) 417, 894
Bsp120I GGGCCC 1 cut(s) 891
Bsp1286I GDGCHC 4 cut(s) 418, 731, 895, 1000
Bsp13I TCCGGA 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 207
Bsp1720I GCTNAGC 1 cut(s) 568
Bsp19I CCATGG 1 cut(s) 722
BspANI GGCC 3 cut(s) 5, 721, 893
BspCNI CTCAG 2 cut(s) 581, 813
BspEI TCCGGA 1 cut(s) 440
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 5 cut(s) 252, 544, 728, 865, 893
BspMAI CTGCAG 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 250
BsrBI CCGCTC 2 cut(s) 422, 860
BsrDI GCAATG 2 cut(s) 36, 210
BsrFI RCCGGY 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 4 cut(s) 133, 158, 803, 962
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 5 cut(s) 126, 712, 722, 738, 894
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 2 cut(s) 712, 722
Bst2UI CCWGG 4 cut(s) 225, 359, 739, 979
Bst4CI ACNGT 6 cut(s) 11, 58, 127, 355, 829, 843
BstACI GRCGYC 1 cut(s) 168
BstAUI TGTACA 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 255, 283, 512, 969
BstDEI CTNAG 3 cut(s) 431, 568, 821
BstDSI CCRYGG 2 cut(s) 126, 722
BstENI CCTNNNNNAGG 1 cut(s) 736
BstF5I GGATG 1 cut(s) 616
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 3 cut(s) 287, 781, 922
BstMAI GTCTC 1 cut(s) 293
BstMCI CGRYCG 1 cut(s) 809
BstMWI GCNNNNNNNGC 3 cut(s) 176, 434, 690
BstNI CCWGG 4 cut(s) 225, 359, 739, 979
BstNSI RCATGY 1 cut(s) 766
BstPAI GACNNNNGTC 1 cut(s) 801
BstSCI CCNGG 6 cut(s) 223, 357, 737, 893, 894, 977
BstSFI CTRYAG 2 cut(s) 255, 520
BstSLI GKGCMC 2 cut(s) 895, 1000
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 3 cut(s) 464, 904, 1009
BsuRI GGCC 3 cut(s) 5, 721, 893
BtgI CCRYGG 2 cut(s) 126, 722
BtsCI GGATG 1 cut(s) 616
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 4 cut(s) 255, 283, 512, 969
CaiI CAGNNNCTG 1 cut(s) 528
CfoI GCGC 3 cut(s) 287, 781, 922
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 3 cut(s) 130, 891, 892
Cfr9I CCCGGG 1 cut(s) 894
CseI GACGC 2 cut(s) 90, 176
CsiI ACCWGGT 1 cut(s) 977
Csp6I GTAC 5 cut(s) 208, 403, 517, 825, 844
CviAII CATG 3 cut(s) 312, 723, 763
CviQI GTAC 5 cut(s) 208, 403, 517, 825, 844
DdeI CTNAG 3 cut(s) 431, 568, 821
EaeI YGGCCR 2 cut(s) 3, 719
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 712, 722
Eco24I GRGCYC 3 cut(s) 418, 731, 895
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 636, 652
Eco88I CYCGRG 2 cut(s) 417, 894
EcoICRI GAGCTC 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 736
EcoRII CCWGG 4 cut(s) 223, 357, 737, 977
EcoT14I CCWWGG 2 cut(s) 712, 722
EcoT38I GRGCYC 3 cut(s) 418, 731, 895
ErhI CCWWGG 2 cut(s) 712, 722
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 3 cut(s) 315, 726, 766
FaqI GGGAC 2 cut(s) 656, 806
FatI CATG 3 cut(s) 311, 722, 762
FauI CCCGC 3 cut(s) 280, 317, 853
FblI GTMKAC 2 cut(s) 549, 831
Fnu4HI GCNGC 7 cut(s) 453, 573, 694, 782, 918, 923, 1023
FokI GGATG 1 cut(s) 623
FriOI GRGCYC 3 cut(s) 418, 731, 895
Fsp4HI GCNGC 7 cut(s) 453, 573, 694, 782, 918, 923, 1023
FspBI CTAG 1 cut(s) 713
GlaI GCGC 3 cut(s) 286, 780, 921
GluI GCNGC 7 cut(s) 453, 573, 694, 782, 918, 923, 1023
HaeIII GGCC 3 cut(s) 5, 721, 893
HapII CCGG 5 cut(s) 138, 282, 441, 637, 895
HgaI GACGC 2 cut(s) 90, 176
HhaI GCGC 3 cut(s) 287, 781, 922
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 3 cut(s) 315, 726, 766
Hin6I GCGC 3 cut(s) 285, 779, 920
HinP1I GCGC 3 cut(s) 285, 779, 920
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 2 cut(s) 316, 621
HpaII CCGG 5 cut(s) 138, 282, 441, 637, 895
HphI GGTGA 2 cut(s) 52, 343
Hpy166II GTNNAC 5 cut(s) 130, 208, 550, 832, 998
Hpy188I TCNGA 9 cut(s) 106, 367, 396, 502, 563, 620, 655, 839, 975
Hpy188III TCNNGA 4 cut(s) 441, 485, 583, 985
Hpy8I GTNNAC 5 cut(s) 130, 208, 550, 832, 998
Hpy99I CGWCG 5 cut(s) 11, 190, 551, 818, 877
HpyAV CCTTC 2 cut(s) 358, 533
HpyCH4III ACNGT 6 cut(s) 11, 58, 127, 355, 829, 843
HpyCH4IV ACGT 3 cut(s) 401, 872, 905
HpyCH4V TGCA 7 cut(s) 83, 215, 257, 468, 598, 684, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 434, 690
HpyF3I CTNAG 3 cut(s) 431, 568, 821
HpySE526I ACGT 3 cut(s) 401, 872, 905
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 3 cut(s) 315, 726, 766
HspAI GCGC 3 cut(s) 285, 779, 920
Kpn2I TCCGGA 1 cut(s) 440
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
LmnI GCTCC 6 cut(s) 413, 542, 726, 857, 863, 976
Lsp1109I GCAGC 3 cut(s) 464, 904, 1009
LweI GCATC 1 cut(s) 166
MabI ACCWGGT 1 cut(s) 977
MaeI CTAG 1 cut(s) 713
MaeII ACGT 3 cut(s) 401, 872, 905
MaeIII GTNAC 4 cut(s) 13, 58, 67, 1015
MbiI CCGCTC 2 cut(s) 422, 860
MboII GAAGA 1 cut(s) 999
MhlI GDGCHC 4 cut(s) 418, 731, 895, 1000
MluCI AATT 3 cut(s) 94, 243, 390
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 3 cut(s) 368, 598, 998
MnlI CCTC 5 cut(s) 82, 395, 607, 633, 907
MroI TCCGGA 1 cut(s) 440
MroNI GCCGGC 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 3 cut(s) 219, 885, 962
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 5 cut(s) 138, 282, 441, 637, 895
MspR9I CCNGG 6 cut(s) 225, 359, 739, 895, 896, 979
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 4 cut(s) 225, 359, 739, 979
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 176, 434, 690
NaeI GCCGGC 1 cut(s) 283
NciI CCSGG 2 cut(s) 895, 896
NcoI CCATGG 1 cut(s) 722
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 3 cut(s) 315, 726, 766
NlaIV GGNNCC 5 cut(s) 252, 544, 728, 865, 893
NmuCI GTSAC 2 cut(s) 58, 1015
NspI RCATGY 1 cut(s) 766
PaeR7I CTCGAG 1 cut(s) 417
PciI ACATGT 1 cut(s) 762
PcsI WCGNNNNNNNCGW 3 cut(s) 299, 543, 764
PctI GAATGC 1 cut(s) 455
PdiI GCCGGC 1 cut(s) 283
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 621
Pfl23II CGTACG 1 cut(s) 402
PkrI GCNGC 7 cut(s) 454, 574, 695, 783, 919, 924, 1024
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 402
PscI ACATGT 1 cut(s) 762
PshAI GACNNNNGTC 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 4 cut(s) 223, 357, 737, 977
PspGI CCWGG 4 cut(s) 223, 357, 737, 977
PspLI CGTACG 1 cut(s) 402
PspN4I GGNNCC 5 cut(s) 252, 544, 728, 865, 893
PspOMI GGGCCC 1 cut(s) 891
PspPI GGNCC 3 cut(s) 130, 891, 892
PspXI VCTCGAGB 1 cut(s) 417
PstI CTGCAG 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 528
RsaI GTAC 5 cut(s) 209, 404, 518, 826, 845
RsaNI GTAC 5 cut(s) 208, 403, 517, 825, 844
RseI CAYNNNNRTG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 418
SalI GTCGAC 1 cut(s) 548
SaqAI TTAA 3 cut(s) 219, 885, 962
SatI GCNGC 7 cut(s) 453, 573, 694, 782, 918, 923, 1023
Sau96I GGNCC 3 cut(s) 130, 891, 892
ScaI AGTACT 2 cut(s) 518, 826
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 6 cut(s) 225, 359, 739, 895, 896, 979
SduI GDGCHC 4 cut(s) 418, 731, 895, 1000
SexAI ACCWGGT 1 cut(s) 977
SfaNI GCATC 1 cut(s) 166
SfcI CTRYAG 2 cut(s) 255, 520
Sfr274I CTCGAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 417
SmaI CCCGGG 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 75, 417
SmoI CTYRAG 2 cut(s) 75, 417
Sse9I AATT 3 cut(s) 94, 243, 390
SspMI CTAG 1 cut(s) 713
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 6 cut(s) 223, 357, 737, 893, 894, 977
StyI CCWWGG 2 cut(s) 712, 722
TaaI ACNGT 6 cut(s) 11, 58, 127, 355, 829, 843
TaiI ACGT 3 cut(s) 404, 875, 908
TaqI TCGA 6 cut(s) 188, 418, 537, 549, 602, 767
TaqII GACCGA 1 cut(s) 795
TasI AATT 3 cut(s) 94, 243, 390
TatI WGTACW 4 cut(s) 207, 516, 824, 843
TauI GCSGC 4 cut(s) 575, 696, 784, 925
TfiI GAWTC 1 cut(s) 621
Tru1I TTAA 3 cut(s) 219, 885, 962
Tru9I TTAA 3 cut(s) 219, 885, 962
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 2 cut(s) 58, 1015
TseI GCWGC 3 cut(s) 452, 917, 1022
Tsp45I GTSAC 2 cut(s) 58, 1015
TspDTI ATGAA 3 cut(s) 544, 613, 931
TspGWI ACGGA 1 cut(s) 992
TspMI CCCGGG 1 cut(s) 894
TspRI CASTG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 996
VpaK11BI GGWCC 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 736
XapI RAATTY 1 cut(s) 243
XceI RCATGY 1 cut(s) 766
XhoI CTCGAG 1 cut(s) 417
XmaI CCCGGG 1 cut(s) 894
XmaJI CCTAGG 1 cut(s) 712
XmiI GTMKAC 2 cut(s) 549, 831
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 1 cut(s) 713
ZrmI AGTACT 2 cut(s) 518, 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.