Rroxscaffold_1G00015260

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003654
Physical Location & Seq
Forward (+)
19038944 .. 19042369
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00015260.1

Sequence Viewer

Length: 1128 bp
ATGGTCGCCGCGCTCCTACACCGGGCAAACGACGTCGTTCAAGACACCGGTGTGGAGAAACTCAACCGGACGGCCGAAGTGGCCGGAGGAGATGTTGAGGTCGAGGGAGAGATCTCCTCGCTCCAACGGCGCAAGGAAGTTGGCATTGGCCTTCTTGCCATGGAGAGACCTAGCGCCGAAGGAACAAACACGAGCACTTCAGCGATTCTCATAACAGTTGACCAATCGGGCAAAGGTGATTACAAGAAAATACAAGAAGCCATTGATTCAGTGGCGTCCAACAACGCAGAGCTCGTGTTTATTAGGGTTAAGCCCGGAGTATATGAAGAAAAAGTTATGGTTCCGGCGGACAAGCCGTTTATAACAATAAGTGGCACAAAGGCAGCTGACACGGTTATAACATGGAACGATAGTGGGGACATATTCGAGTCTCCAACTTTCTCTGTCCTGGCCTCTGATTTCATAGGGCGTTACCTCACAATTCAGAATACATATGGAGCTGGTGTGAAAGCGGTGGCATTAAGGGTGTCTGGAGATAGAGCAGCATTCTATGGGTGCAGAATTTTGTCTCATCAAGACACCCTCCTTGATGATACTGGGAGGCACTACTACAACAACTGTTACATTGAAGGAGATGTAGACTTCATATGTGGAAATGCTGCTTCTTTCTTTGAGAAGTGCCATTTGCATTCTCTTTCGGTAGGAGCCGGGGCCATAACGGCCCAACGGAGAATGTCCCCGGCTGAGGACACTGGGTTCATGTTCTTCGGGTGCAAGATCACCGGTGTCAAAACAGCAATGTTGGGAAGGCCTTGGGGTACATATTCAAGAGTGGTCTTTGCCTTTACTTACATGACTAGCGCCATACTGCCACAAGGTTGGGACGATTGGGGAAACTCAGCCGACCAATTAAGAACCGTATACTATGGGCAGTATAAATGTTCTGGACGGGGAGCAACTACATCGAAACGAGTAGAATGGGCACGCGACCTTACGAGCGAAGAAGTCGAACCCTTCTTGACCAAGGACATGATTGATGCCAATGCTTGGTTAAGATATGTGTATCAAGTACATGGAGATTTAGCAGATAATGATTTCTTGGATTTGTTTGGAATTGATTTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

375

Amino Acids

41.21

Weight (kDa)

4.99

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 71 - 347 1.1e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 362, 398
AatII GACGTC 1 cut(s) 36
AccB7I CCANNNNNTGG 1 cut(s) 1047
AccI GTMKAC 2 cut(s) 639, 921
AccII CGCG 2 cut(s) 11, 987
AciI CCGC 3 cut(s) 9, 347, 512
AcoI YGGCCR 2 cut(s) 72, 81
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 1 cut(s) 183
AcyI GRCGYC 2 cut(s) 33, 275
AfaI GTAC 2 cut(s) 820, 1071
AfiI CCNNNNNNNGG 3 cut(s) 22, 745, 1047
AgeI ACCGGT 2 cut(s) 47, 782
AgsI TTSAA 4 cut(s) 41, 629, 828, 1123
AjnI CCWGG 1 cut(s) 447
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AleI CACNNNNGTG 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 3 cut(s) 292, 386, 500
AluI AGCT 3 cut(s) 292, 386, 500
Alw21I GWGCWC 2 cut(s) 197, 294
Alw26I GTCTC 3 cut(s) 160, 435, 573
AoxI GGCC 7 cut(s) 72, 81, 148, 450, 711, 720, 809
ApeKI GCWGC 3 cut(s) 383, 542, 659
ApoI RAATTY 1 cut(s) 561
AsiGI ACCGGT 2 cut(s) 47, 782
AspLEI GCGC 4 cut(s) 13, 132, 176, 863
AspS9I GGNCC 2 cut(s) 711, 721
AsuC2I CCSGG 4 cut(s) 23, 315, 709, 740
AsuHPI GGTGA 2 cut(s) 248, 772
BaeGI GKGCMC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 294
BauI CACGAG 2 cut(s) 190, 293
Bbv12I GWGCWC 2 cut(s) 197, 294
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 3 cut(s) 395, 554, 646
BceAI ACGGC 4 cut(s) 87, 143, 340, 735
BcgI CGANNNNNNTGC 2 cut(s) 945, 979
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 4 cut(s) 23, 315, 709, 740
BcoDI GTCTC 3 cut(s) 160, 435, 573
BfaI CTAG 2 cut(s) 171, 858
BfoI RGCGCY 2 cut(s) 177, 864
BglI GCCNNNNNGGC 2 cut(s) 80, 719
BglII AGATCT 1 cut(s) 111
BisI GCNGC 4 cut(s) 9, 384, 543, 660
BlsI GCNGC 4 cut(s) 10, 385, 544, 661
Bme1390I CCNGG 5 cut(s) 23, 315, 449, 709, 740
BmgT120I GGNCC 2 cut(s) 711, 721
BmiI GGNNCC 3 cut(s) 342, 706, 712
BmrFI CCNGG 5 cut(s) 23, 315, 449, 709, 740
BmrI ACTGGG 2 cut(s) 606, 762
BmsI GCATC 1 cut(s) 1027
BmuI ACTGGG 2 cut(s) 606, 762
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 744
BpuMI CCSGG 4 cut(s) 23, 315, 709, 740
BsaHI GRCGYC 2 cut(s) 33, 275
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 5 cut(s) 159, 708, 738, 812, 1023
BsaWI WCCGGW 3 cut(s) 47, 66, 782
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 745, 1047
Bse118I RCCGGY 2 cut(s) 47, 782
Bse1I ACTGG 2 cut(s) 601, 757
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 159, 708, 738, 812, 1023
BseLI CCNNNNNNNGG 3 cut(s) 22, 745, 1047
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 735, 912
BseNI ACTGG 2 cut(s) 601, 757
BseRI GAGGAG 2 cut(s) 102, 106
BseSI GKGCMC 1 cut(s) 985
BseX3I CGGCCG 1 cut(s) 72
BseXI GCAGC 3 cut(s) 395, 554, 646
BsgI GTGCAG 1 cut(s) 577
Bsh1236I CGCG 2 cut(s) 11, 987
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 7 cut(s) 74, 83, 150, 452, 713, 722, 811
BshTI ACCGGT 2 cut(s) 47, 782
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 197, 294
BsiSI CCGG 9 cut(s) 22, 48, 67, 84, 315, 344, 708, 740, 783
BslFI GGGAC 3 cut(s) 431, 721, 896
BslI CCNNNNNNNGG 3 cut(s) 22, 745, 1047
BsmAI GTCTC 3 cut(s) 160, 435, 573
BsmFI GGGAC 3 cut(s) 431, 721, 896
BsmI GAATGC 2 cut(s) 545, 688
BsnI GGCC 7 cut(s) 74, 83, 150, 452, 713, 722, 811
Bso31I GGTCTC 1 cut(s) 160
Bsp1286I GDGCHC 3 cut(s) 197, 294, 985
Bsp143I GATC 2 cut(s) 111, 777
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 3 cut(s) 9, 347, 512
BspANI GGCC 7 cut(s) 74, 83, 150, 452, 713, 722, 811
BspCNI CTCAG 2 cut(s) 736, 911
BspFNI CGCG 2 cut(s) 11, 987
BspLI GGNNCC 3 cut(s) 342, 706, 712
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 2 cut(s) 47, 782
BsrI ACTGG 2 cut(s) 601, 757
BssAI RCCGGY 2 cut(s) 47, 782
BssECI CCNNGG 5 cut(s) 159, 708, 738, 812, 1023
BssMI GATC 2 cut(s) 111, 777
BssNAI GTATAC 1 cut(s) 922
BssNI GRCGYC 2 cut(s) 33, 275
BssSI CACGAG 2 cut(s) 190, 293
BssT1I CCWWGG 3 cut(s) 159, 812, 1023
Bst1107I GTATAC 1 cut(s) 922
Bst2BI CACGAG 2 cut(s) 190, 293
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 4 cut(s) 217, 394, 620, 919
BstACI GRCGYC 2 cut(s) 33, 275
BstC8I GCNNGC 1 cut(s) 985
BstDEI CTNAG 2 cut(s) 744, 898
BstDSI CCRYGG 1 cut(s) 159
BstFNI CGCG 2 cut(s) 11, 987
BstH2I RGCGCY 2 cut(s) 177, 864
BstHHI GCGC 4 cut(s) 13, 132, 176, 863
BstKTI GATC 2 cut(s) 114, 780
BstMAI GTCTC 3 cut(s) 160, 435, 573
BstMBI GATC 2 cut(s) 111, 777
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 80, 127, 719
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 5 cut(s) 21, 313, 447, 707, 738
BstSLI GKGCMC 1 cut(s) 985
BstUI CGCG 2 cut(s) 11, 987
BstV1I GCAGC 3 cut(s) 395, 554, 646
BstX2I RGATCY 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 879
BstYI RGATCY 1 cut(s) 111
BstZ17I GTATAC 1 cut(s) 922
BstZI CGGCCG 1 cut(s) 72
BsuRI GGCC 7 cut(s) 74, 83, 150, 452, 713, 722, 811
BtgI CCRYGG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 276, 750
Cac8I GCNNGC 1 cut(s) 985
CfoI GCGC 4 cut(s) 13, 132, 176, 863
Cfr10I RCCGGY 2 cut(s) 47, 782
Cfr13I GGNCC 2 cut(s) 711, 721
CseI GACGC 1 cut(s) 264
Csp6I GTAC 2 cut(s) 819, 1070
CspAI ACCGGT 2 cut(s) 47, 782
CviAII CATG 6 cut(s) 160, 402, 760, 853, 1030, 1073
CviQI GTAC 2 cut(s) 819, 1070
DdeI CTNAG 2 cut(s) 744, 898
DpnI GATC 2 cut(s) 113, 779
DpnII GATC 2 cut(s) 111, 777
EaeI YGGCCR 2 cut(s) 72, 81
EagI CGGCCG 1 cut(s) 72
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 72
Eco130I CCWWGG 3 cut(s) 159, 812, 1023
Eco147I AGGCCT 1 cut(s) 811
Eco24I GRGCYC 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 160
Eco52I CGGCCG 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 3 cut(s) 159, 812, 1023
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 3 cut(s) 159, 812, 1023
FaeI CATG 6 cut(s) 163, 405, 763, 856, 1033, 1076
FaqI GGGAC 3 cut(s) 431, 721, 896
FatI CATG 6 cut(s) 159, 401, 759, 852, 1029, 1072
FauNDI CATATG 2 cut(s) 493, 647
FblI GTMKAC 2 cut(s) 639, 921
Fnu4HI GCNGC 4 cut(s) 9, 384, 543, 660
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 4 cut(s) 9, 384, 543, 660
FspBI CTAG 2 cut(s) 171, 858
GlaI GCGC 4 cut(s) 12, 131, 175, 862
GluI GCNGC 4 cut(s) 9, 384, 543, 660
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 2 cut(s) 177, 864
HaeIII GGCC 7 cut(s) 74, 83, 150, 452, 713, 722, 811
HapII CCGG 9 cut(s) 22, 48, 67, 84, 315, 344, 708, 740, 783
HgaI GACGC 1 cut(s) 264
HhaI GCGC 4 cut(s) 13, 132, 176, 863
Hin1I GRCGYC 2 cut(s) 33, 275
Hin1II CATG 6 cut(s) 163, 405, 763, 856, 1033, 1076
Hin6I GCGC 4 cut(s) 11, 130, 174, 861
HinP1I GCGC 4 cut(s) 11, 130, 174, 861
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 205, 266, 428
HpaII CCGG 9 cut(s) 22, 48, 67, 84, 315, 344, 708, 740, 783
HphI GGTGA 2 cut(s) 248, 772
Hpy166II GTNNAC 3 cut(s) 220, 640, 922
Hpy188I TCNGA 2 cut(s) 457, 486
Hpy188III TCNNGA 6 cut(s) 41, 531, 575, 828, 945, 1018
Hpy8I GTNNAC 3 cut(s) 220, 640, 922
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 5 cut(s) 161, 173, 623, 801, 1024
HpyCH4III ACNGT 4 cut(s) 217, 394, 620, 919
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 3 cut(s) 558, 688, 774
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 127, 719
HpyF3I CTNAG 2 cut(s) 744, 898
HpySE526I ACGT 1 cut(s) 33
Hsp92I GRCGYC 2 cut(s) 33, 275
Hsp92II CATG 6 cut(s) 163, 405, 763, 856, 1033, 1076
HspAI GCGC 4 cut(s) 11, 130, 174, 861
Kzo9I GATC 2 cut(s) 111, 777
LmnI GCTCC 5 cut(s) 18, 126, 497, 704, 953
Lsp1109I GCAGC 3 cut(s) 395, 554, 646
LweI GCATC 1 cut(s) 1027
MaeI CTAG 2 cut(s) 171, 858
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 470, 620
MalI GATC 2 cut(s) 113, 779
MboI GATC 2 cut(s) 111, 777
MboII GAAGA 3 cut(s) 338, 757, 1013
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 3 cut(s) 197, 294, 985
MluCI AATT 5 cut(s) 480, 561, 908, 1113, 1123
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 3 cut(s) 148, 303, 458
MnlI CCTC 9 cut(s) 80, 91, 97, 127, 463, 485, 593, 594, 739
MseI TTAA 4 cut(s) 309, 521, 911, 1052
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 9 cut(s) 22, 48, 67, 84, 315, 344, 708, 740, 783
MspR9I CCNGG 5 cut(s) 23, 315, 449, 709, 740
Mva1269I GAATGC 2 cut(s) 545, 688
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 2 cut(s) 11, 987
MwoI GCNNNNNNNGC 3 cut(s) 80, 127, 719
NciI CCSGG 4 cut(s) 23, 315, 709, 740
NcoI CCATGG 1 cut(s) 159
NdeI CATATG 2 cut(s) 493, 647
NdeII GATC 2 cut(s) 111, 777
NlaIII CATG 6 cut(s) 163, 405, 763, 856, 1033, 1076
NlaIV GGNNCC 3 cut(s) 342, 706, 712
OliI CACNNNNGTG 1 cut(s) 50
PceI AGGCCT 1 cut(s) 811
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 2 cut(s) 545, 688
PfeI GAWTC 2 cut(s) 205, 266
PflMI CCANNNNNTGG 1 cut(s) 1047
PinAI ACCGGT 2 cut(s) 47, 782
PkrI GCNGC 4 cut(s) 10, 385, 544, 661
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PsiI TTATAA 2 cut(s) 362, 398
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 3 cut(s) 342, 706, 712
PspPI GGNCC 2 cut(s) 711, 721
PsuI RGATCY 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 820, 1071
RsaNI GTAC 2 cut(s) 819, 1070
RseI CAYNNNNRTG 1 cut(s) 50
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 4 cut(s) 309, 521, 911, 1052
SatI GCNGC 4 cut(s) 9, 384, 543, 660
Sau3AI GATC 2 cut(s) 111, 777
Sau96I GGNCC 2 cut(s) 711, 721
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 5 cut(s) 23, 315, 449, 709, 740
SduI GDGCHC 3 cut(s) 197, 294, 985
SfaNI GCATC 1 cut(s) 1027
SfiI GGCCNNNNNGGCC 2 cut(s) 80, 719
SgrAI CRCCGGYG 2 cut(s) 47, 782
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 5 cut(s) 480, 561, 908, 1113, 1123
SseBI AGGCCT 1 cut(s) 811
SsiI CCGC 3 cut(s) 9, 347, 512
SspMI CTAG 2 cut(s) 171, 858
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 811
StyD4I CCNGG 5 cut(s) 21, 313, 447, 707, 738
StyI CCWWGG 3 cut(s) 159, 812, 1023
TaaI ACNGT 4 cut(s) 217, 394, 620, 919
TaiI ACGT 1 cut(s) 36
TaqI TCGA 4 cut(s) 102, 426, 965, 1008
TasI AATT 5 cut(s) 480, 561, 908, 1113, 1123
TatI WGTACW 1 cut(s) 1069
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 205, 266
Tru1I TTAA 4 cut(s) 309, 521, 911, 1052
Tru9I TTAA 4 cut(s) 309, 521, 911, 1052
TscAI CASTG 2 cut(s) 276, 757
TseI GCWGC 3 cut(s) 383, 542, 659
TspDTI ATGAA 4 cut(s) 339, 451, 634, 748
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 2 cut(s) 276, 757
Van91I CCANNNNNTGG 1 cut(s) 1047
XapI RAATTY 1 cut(s) 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XmiI GTMKAC 2 cut(s) 639, 921
XspI CTAG 2 cut(s) 171, 858
ZraI GACGTC 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.