MD08G1029800.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003654
Physical Location & Seq
Reverse (-)
2156404 .. 2158458
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1029800.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGTGGCTGTTTGTGGCACTTCTATGACCAAGTGCTTCTGTGTCATGGCTTCATTGTATGTTCTTCTAGCGATTTCTCAAGCAGAAGCAGACACAACTCAGGGGTCCACGGCAATACTAATAACCGTGGATCAGACTGGGAAAGGCGATTACGAGAAAATACAAGATGCCATCGACGCCGTGCCGTCGAATAACGTCGATCTTGTGTTCGTTAAGGTTAACCCTGGAGTCTACAAAGAAAAAATTACTGTCCCCGCAGACAAACCCTTTATTACAATCAGTGGCACAACGGCAGGTGGCACTGTTATTACGTGGAACGACAGTGGAAATATATTTGAATCTGCAACTTTCTCTGTGTTGGCTTCGGATTTTATAGGGCGCTACCTCACAATTCAGAACACTTATGGAAGGGGTGATAAAGCAGTGGCAGTAAGAGTCTCAGGAGATAGAGCAGCGTTTTATGTGTGCAGAATTTTGTCTTACCAAGATACCCTACTTGACGATACCGGCAGGCATTACTACAACAACTGTTATATTGAAGGAGCCACTGATTTTATATGTGGGAATGCTGCTTCCTTTTTTGAGTGCCATTTGCATTCGATTTCGGACGGAGGGGGTGCCATAACAGCCCAACGGAGGATGTCACCTTCAGAGGACACTGGGTTCATGTTCTTTGGGTGCAAGATCACTGGCGTCAAAACGGCAGTGTTAGGAAGGCCATGGGGTGTCTACTCCAGAGTCGTTTTTGCCTTCACTTACATGCCTAATGCCATACTCCCTCAGGGTTGGGACAACTGGGGACACTCAGCTGACCAATTAAGTTCTGTTTACTATGGACAATACGAATGTTCTGGACCTGGAGCTGTGACATCAAAAAGAGCTGACTGGGCACGAGTCCTAACGAGTGAAGAAGCTGCACCTTTCATAACAAAGGACTCCATTGATGCAAAAGCTTGGATTAGGGCAACGCCTAAGAGTTTCAAGAAGAAGGCATTTTCCACCAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

337

Amino Acids

36.49

Weight (kDa)

6.43

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 41 - 314 8.9e-66 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 617
AccI GTMKAC 2 cut(s) 231, 729
AciI CCGC 1 cut(s) 255
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 2 cut(s) 177, 693
AfiI CCNNNNNNNGG 1 cut(s) 636
AgsI TTSAA 3 cut(s) 338, 539, 982
AjnI CCWGG 2 cut(s) 223, 856
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
AluBI AGCT 5 cut(s) 809, 863, 881, 914, 953
AluI AGCT 5 cut(s) 809, 863, 881, 914, 953
Alw26I GTCTC 1 cut(s) 442
AlwI GGATC 1 cut(s) 138
AoxI GGCC 1 cut(s) 716
ApeKI GCWGC 3 cut(s) 452, 569, 914
ApoI RAATTY 1 cut(s) 471
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 105, 854
AsuHPI GGTGA 2 cut(s) 425, 636
AvaII GGWCC 2 cut(s) 105, 854
AxyI CCTNAGG 1 cut(s) 780
BaeGI GKGCMC 1 cut(s) 892
BanI GGYRCC 1 cut(s) 617
BauI CACGAG 1 cut(s) 891
BbvI GCAGC 3 cut(s) 464, 556, 901
BccI CCATC 1 cut(s) 179
BceAI ACGGC 5 cut(s) 126, 164, 169, 306, 717
BciT130I CCWGG 2 cut(s) 225, 858
BcoDI GTCTC 1 cut(s) 442
BfaI CTAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 453, 570, 915
BlsI GCNGC 3 cut(s) 454, 571, 916
Bme1390I CCNGG 2 cut(s) 225, 858
Bme18I GGWCC 2 cut(s) 105, 854
BmgT120I GGNCC 2 cut(s) 105, 854
BmiI GGNNCC 3 cut(s) 106, 544, 619
BmrFI CCNGG 2 cut(s) 225, 858
BmrI ACTGGG 4 cut(s) 147, 669, 805, 895
BmsI GCATC 2 cut(s) 157, 934
BmuI ACTGGG 4 cut(s) 147, 669, 805, 895
BpmI CTGGAG 3 cut(s) 246, 718, 879
BpuEI CTTGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 312
BsaHI GRCGYC 2 cut(s) 177, 693
BsaJI CCNNGG 4 cut(s) 108, 126, 223, 719
Bsc4I CCNNNNNNNGG 1 cut(s) 636
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 5 cut(s) 142, 664, 694, 800, 890
Bse21I CCTNAGG 1 cut(s) 780
BseBI CCWGG 2 cut(s) 225, 858
BseDI CCNNGG 4 cut(s) 108, 126, 223, 719
BseGI GGATG 1 cut(s) 645
BseLI CCNNNNNNNGG 1 cut(s) 636
BseMII CTCAG 4 cut(s) 113, 453, 794, 819
BseNI ACTGG 5 cut(s) 142, 664, 694, 800, 890
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 3 cut(s) 464, 556, 901
BsgI GTGCAG 2 cut(s) 487, 900
BshFI GGCC 1 cut(s) 718
BshNI GGYRCC 1 cut(s) 617
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 3 cut(s) 236, 803, 813
BslI CCNNNNNNNGG 1 cut(s) 636
BsmAI GTCTC 1 cut(s) 442
BsmFI GGGAC 3 cut(s) 236, 803, 813
BsmI GAATGC 2 cut(s) 571, 595
BsnI GGCC 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 892
Bsp143I GATC 3 cut(s) 130, 199, 684
Bsp19I CCATGG 1 cut(s) 719
BspACI CCGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 718
BspCNI CTCAG 4 cut(s) 112, 452, 793, 818
BspLI GGNNCC 3 cut(s) 106, 544, 619
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 5 cut(s) 142, 664, 694, 800, 890
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 4 cut(s) 108, 126, 223, 719
BssMI GATC 3 cut(s) 130, 199, 684
BssNI GRCGYC 2 cut(s) 177, 693
BssSI CACGAG 1 cut(s) 891
BssT1I CCWWGG 1 cut(s) 719
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 225, 858
Bst4CI ACNGT 5 cut(s) 127, 250, 304, 323, 530
BstACI GRCGYC 2 cut(s) 177, 693
BstBAI YACGTR 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 5 cut(s) 99, 439, 780, 805, 972
BstDSI CCRYGG 3 cut(s) 108, 126, 719
BstF5I GGATG 1 cut(s) 645
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 3 cut(s) 133, 202, 687
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 3 cut(s) 130, 199, 684
BstMWI GCNNNNNNNGC 3 cut(s) 176, 626, 887
BstNI CCWGG 2 cut(s) 225, 858
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 2 cut(s) 223, 856
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 3 cut(s) 464, 556, 901
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 1 cut(s) 718
BtgI CCRYGG 3 cut(s) 108, 126, 719
BtsCI GGATG 1 cut(s) 645
BtsI GCAGTG 2 cut(s) 429, 711
BtsIMutI CAGTG 8 cut(s) 286, 300, 328, 429, 546, 657, 687, 711
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 512
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 2 cut(s) 105, 854
CseI GACGC 2 cut(s) 185, 682
CviAII CATG 4 cut(s) 46, 667, 720, 760
DdeI CTNAG 5 cut(s) 99, 439, 780, 805, 972
DpnI GATC 3 cut(s) 132, 201, 686
DpnII GATC 3 cut(s) 130, 199, 684
Eco130I CCWWGG 1 cut(s) 719
Eco47I GGWCC 2 cut(s) 105, 854
Eco57I CTGAAG 1 cut(s) 633
Eco81I CCTNAGG 1 cut(s) 780
EcoRII CCWGG 2 cut(s) 223, 856
EcoT14I CCWWGG 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 4 cut(s) 49, 670, 723, 763
FaqI GGGAC 3 cut(s) 236, 803, 813
FatI CATG 4 cut(s) 45, 666, 719, 759
FauI CCCGC 1 cut(s) 262
FblI GTMKAC 2 cut(s) 231, 729
Fnu4HI GCNGC 3 cut(s) 453, 570, 915
FokI GGATG 1 cut(s) 652
Fsp4HI GCNGC 3 cut(s) 453, 570, 915
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 380
GluI GCNGC 3 cut(s) 453, 570, 915
GsuI CTGGAG 3 cut(s) 246, 718, 879
HaeII RGCGCY 1 cut(s) 382
HaeIII GGCC 1 cut(s) 718
HapII CCGG 1 cut(s) 507
HgaI GACGC 2 cut(s) 185, 682
HhaI GCGC 1 cut(s) 381
Hin1I GRCGYC 2 cut(s) 177, 693
Hin1II CATG 4 cut(s) 49, 670, 723, 763
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 6 cut(s) 228, 338, 435, 738, 894, 935
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 507
HphI GGTGA 2 cut(s) 425, 636
Hpy166II GTNNAC 5 cut(s) 108, 220, 232, 730, 829
Hpy188I TCNGA 5 cut(s) 135, 367, 396, 607, 652
Hpy188III TCNNGA 4 cut(s) 441, 735, 852, 982
Hpy8I GTNNAC 5 cut(s) 108, 220, 232, 730, 829
Hpy99I CGWCG 3 cut(s) 179, 190, 200
HpyAV CCTTC 6 cut(s) 402, 533, 657, 708, 760, 982
HpyCH4III ACNGT 5 cut(s) 127, 250, 304, 323, 530
HpyCH4IV ACGT 2 cut(s) 195, 311
HpyCH4V TGCA 6 cut(s) 344, 468, 595, 681, 917, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 626, 887
HpyF3I CTNAG 5 cut(s) 99, 439, 780, 805, 972
HpySE526I ACGT 2 cut(s) 195, 311
Hsp92I GRCGYC 2 cut(s) 177, 693
Hsp92II CATG 4 cut(s) 49, 670, 723, 763
HspAI GCGC 1 cut(s) 379
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 130, 199, 684
LmnI GCTCC 2 cut(s) 542, 860
Lsp1109I GCAGC 3 cut(s) 464, 556, 901
LweI GCATC 2 cut(s) 157, 934
MaeI CTAG 1 cut(s) 68
MaeII ACGT 2 cut(s) 195, 311
MaeIII GTNAC 2 cut(s) 642, 865
MalI GATC 3 cut(s) 132, 201, 686
MboI GATC 3 cut(s) 130, 199, 684
MboII GAAGA 3 cut(s) 56, 920, 997
MhlI GDGCHC 1 cut(s) 892
MluCI AATT 5 cut(s) 243, 390, 471, 815, 1006
MlyI GAGTC 5 cut(s) 237, 444, 747, 903, 929
MnlI CCTC 5 cut(s) 395, 605, 630, 646, 789
MseI TTAA 3 cut(s) 213, 219, 818
MslI CAYNNNNRTG 2 cut(s) 23, 758
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 2 cut(s) 225, 858
Mva1269I GAATGC 2 cut(s) 571, 595
MvaI CCWGG 2 cut(s) 225, 858
MwoI GCNNNNNNNGC 3 cut(s) 176, 626, 887
NcoI CCATGG 1 cut(s) 719
NdeII GATC 3 cut(s) 130, 199, 684
NlaIII CATG 4 cut(s) 49, 670, 723, 763
NlaIV GGNNCC 3 cut(s) 106, 544, 619
NmuCI GTSAC 2 cut(s) 642, 865
NspI RCATGY 1 cut(s) 763
PaqCI CACCTGC 1 cut(s) 284
PctI GAATGC 2 cut(s) 571, 595
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 3 cut(s) 454, 571, 916
PleI GAGTC 5 cut(s) 236, 443, 746, 902, 929
PpsI GAGTC 5 cut(s) 236, 443, 746, 902, 929
Ppu21I YACGTR 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 223, 856
PspGI CCWGG 2 cut(s) 223, 856
PspN4I GGNNCC 3 cut(s) 106, 544, 619
PspPI GGNCC 2 cut(s) 105, 854
PvuII CAGCTG 1 cut(s) 809
RseI CAYNNNNRTG 2 cut(s) 23, 758
SaqAI TTAA 3 cut(s) 213, 219, 818
SatI GCNGC 3 cut(s) 453, 570, 915
Sau3AI GATC 3 cut(s) 130, 199, 684
Sau96I GGNCC 2 cut(s) 105, 854
SchI GAGTC 5 cut(s) 237, 444, 747, 903, 929
ScrFI CCNGG 2 cut(s) 225, 858
SduI GDGCHC 1 cut(s) 892
SfaNI GCATC 2 cut(s) 157, 934
SinI GGWCC 2 cut(s) 105, 854
SmiMI CAYNNNNRTG 2 cut(s) 23, 758
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 5 cut(s) 243, 390, 471, 815, 1006
SsiI CCGC 1 cut(s) 255
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 223, 856
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 5 cut(s) 127, 250, 304, 323, 530
TaiI ACGT 2 cut(s) 198, 314
TaqI TCGA 4 cut(s) 174, 188, 198, 599
TasI AATT 5 cut(s) 243, 390, 471, 815, 1006
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 3 cut(s) 213, 219, 818
Tru9I TTAA 3 cut(s) 213, 219, 818
TscAI CASTG 8 cut(s) 286, 307, 328, 429, 553, 664, 694, 711
TseFI GTSAC 2 cut(s) 642, 865
TseI GCWGC 3 cut(s) 452, 569, 914
Tsp45I GTSAC 2 cut(s) 642, 865
TspDTI ATGAA 3 cut(s) 42, 655, 913
TspGWI ACGGA 2 cut(s) 624, 649
TspRI CASTG 8 cut(s) 286, 307, 328, 429, 553, 664, 694, 711
VpaK11BI GGWCC 2 cut(s) 105, 854
XapI RAATTY 1 cut(s) 471
XceI RCATGY 1 cut(s) 763
XmiI GTMKAC 2 cut(s) 231, 729
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.