Rh6BG420800

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003654
Physical Location & Seq
Reverse (-)
64436184 .. 64437980
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG420800.1

Sequence Viewer

Length: 1797 bp
ATGGCGCGTAGGGCTCTAGTTAGAATGATTGTCATAATTTTGTATGTTTTCGTCACTATTTCTCAAGCGGAAAATATAATAACAGTTGATCAATCCGGTAACGGTCATTACGGTAAAATACAAGATGCTATTGATTCTGTTCCATCAAACAATAAGGATCCTTTGACTATTTCGATCAAGCCTGGAACTTACAAAGAAAGTATTACCATACCTCCAGACAAGCCATTCATAACATTAAGTGGTACTACTACAGAAGCAAGTGATGTGATGATAACGTGGGATCAGAGAGGGGACATTGATAAAGTTGCAACATTTCATGTTTCGGCTTCCGACTTCGTAGCGCGCTACATAACAATTCAAAATACATTCGGGCCTGGTCATCAAGCAGTTGCGCTAAGGGTCTCCGGCGATAGAATAGCGTTTTATGACTGCTCAATTTTAAGTTATCAGGATACTCTATTTGACGAAATAGGCAGACACTACTACGAAAATTGTTACATTGAAGGAGTTGTTGATTTTATATTTGGAAGCGCCAGTTCTTTCTTTCAAAATTGTCACATACATTCGCCTACAGGAGGATATATTACAGCGCAACGAAGAGAAGCGTCAACAGATATTGGAGGGTTTGTGTTCTTTAAGTGTCAAATTACTGGCAAAGGCGAAAAAACATTTTTAGGAAGACCATGGGTGGGATCCTATGCTAGGGTCATATTTGCCTACACTAACATGGAAGATGTGATAGAACCACTAGGTTGGTCTCGTTATTCTGATGGATCCACAGAACAAGAATTAATGACAAACTCATTTTGTGGACAATTCAAATGCGACGGACTAGGAGCAACAACATCCAACAGGGTTAAATGGGCGCGAAAATTCAGTGAGAAACAAATTGCAAATTTTTTGACAACAGACTTTATCAATGGAAAGGATTGGATAAAGTCTACGCCGGTAACAGCGTCGCCTCCACCTAAAAAGGAGACGCCGTCATTGCCTCCATCGGTACTGGATACACCGCCACCCTCACCACCACCTCCCATGAAGCCGTCTGCACCACCACCAGCACCGGTCATCCCGGCACCTGTAATCCCGTCTCCACCACCACAAGCCCCGGTGGTACCAACTTCCCCACCACCGGTGATTCCATTAGTTTCCCCACCAGCACCGGCGGTGATTCCATTAGTTTCCCCACCAGCACCCGTGGTGATTTTATCTCCGCCAGCACCAGCACCCGTGGTGATTTTATCTCCGCCAGCACCAGCACCCGTGATCCCGTCGTCGCCACCACCGACAACGGCACCACCAGTGATTCCGTTGTTGCCCCCACCGGCACCGGTGGTGATTCCATCGTCGCCCCCACCAGCACCAGTGGCGATCCCGTCGTCGCCACCATCGACACCCGTGACCCCGTGGTTCCCGTGGTTCGCACCACCGGCACTGGTGATACCGACGTTGCCACCACTGGTAAATCCATTTTCACCACCACCGGTACATCCATTTTCACCACCACCGGTACCGATGATTCCGTTGTTGCCCCCACGGGCACCGGTGGTGATCTTCTCACCAGGGACACCCGTGATCTCGTGGTTCGCACCACCGCCACCATCACCACGAGAGAGGGTGATTATAACTCCGTGGTCGCCTCCACCAACATACATGGACGGCAGAGCTCAAGAAGGTGAACCTATCATCATGTATTATTATTCCTCGGGGGCATCCTCTTCAATCTTTAAGAATTGTTTCAGCGGCATTGGGACGCTGGCTCTAACCTCGGCAGTTTCACTATACTTGTTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

598

Amino Acids

64.09

Weight (kDa)

6.4

Isoelectric Point (pI)

68.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 308 5.4e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1625
Acc65I GGTACC 2 cut(s) 1114, 1510
AccB1I GGYRCC 6 cut(s) 1075, 1114, 1294, 1327, 1510, 1540
AccI GTMKAC 1 cut(s) 941
AccII CGCG 3 cut(s) 7, 343, 868
AciI CCGC 7 cut(s) 68, 1013, 1166, 1214, 1247, 1595, 1743
AclWI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1261, 1366
AcsI RAATTY 2 cut(s) 872, 895
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 5 cut(s) 244, 1002, 1116, 1488, 1512
AfiI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
AgeI ACCGGT 6 cut(s) 1063, 1132, 1330, 1483, 1507, 1543
AgsI TTSAA 5 cut(s) 359, 503, 548, 820, 1722
AjnI CCWGG 3 cut(s) 181, 373, 1561
AluBI AGCT 1 cut(s) 1667
AluI AGCT 1 cut(s) 1667
Alw21I GWGCWC 1 cut(s) 1669
Alw26I GTCTC 4 cut(s) 406, 762, 971, 1095
AlwI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1261, 1366
Ama87I CYCGRG 1 cut(s) 1705
AoxI GGCC 2 cut(s) 371, 1791
ApoI RAATTY 2 cut(s) 872, 895
AseI ATTAAT 1 cut(s) 791
AsiGI ACCGGT 6 cut(s) 1063, 1132, 1330, 1483, 1507, 1543
Asp700I GAANNNNTTC 1 cut(s) 1736
Asp718I GGTACC 2 cut(s) 1114, 1510
AspLEI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 1073, 1109
AvaI CYCGRG 1 cut(s) 1705
BaeGI GKGCMC 1 cut(s) 1543
BaeI ACNNNNGTAYC 2 cut(s) 1433, 1466
BamHI GGATCC 3 cut(s) 157, 692, 773
BanI GGYRCC 6 cut(s) 1075, 1114, 1294, 1327, 1510, 1540
BanII GRGCYC 2 cut(s) 16, 1669
BauI CACGAG 2 cut(s) 1579, 1608
BbsI GAAGAC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 1669
BccI CCATC 6 cut(s) 151, 764, 1003, 1351, 1396, 1609
BceAI ACGGC 4 cut(s) 967, 1027, 1308, 1675
BciT130I CCWGG 3 cut(s) 183, 375, 1563
BciVI GTATCC 2 cut(s) 445, 1000
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 1073, 1109
BcoDI GTCTC 4 cut(s) 406, 762, 971, 1095
BfaI CTAG 4 cut(s) 17, 702, 749, 833
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 2 cut(s) 445, 1000
BisI GCNGC 1 cut(s) 1744
BlsI GCNGC 1 cut(s) 1745
Bme1390I CCNGG 5 cut(s) 183, 375, 1073, 1109, 1563
BmeT110I CYCGRG 1 cut(s) 1705
BmgT120I GGNCC 1 cut(s) 371
BmrFI CCNGG 5 cut(s) 183, 375, 1073, 1109, 1563
BmsI GCATC 2 cut(s) 115, 1721
BpiI GAAGAC 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 48, 1653
BpuMI CCSGG 2 cut(s) 1073, 1109
BsaHI GRCGYC 1 cut(s) 980
BsaI GGTCTC 2 cut(s) 406, 762
BsaWI WCCGGW 7 cut(s) 95, 1063, 1132, 1330, 1483, 1507, 1543
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 612, 642, 968, 998
Bsc4I CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
Bse1I ACTGG 7 cut(s) 534, 655, 1008, 1301, 1364, 1440, 1464
Bse3DI GCAATG 1 cut(s) 986
BseBI CCWGG 3 cut(s) 183, 375, 1563
BseGI GGATG 4 cut(s) 845, 1068, 1489, 1712
BseLI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
BseMI GCAATG 1 cut(s) 986
BseNI ACTGG 7 cut(s) 534, 655, 1008, 1301, 1364, 1440, 1464
BsePI GCGCGC 1 cut(s) 341
BseSI GKGCMC 1 cut(s) 1543
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 3 cut(s) 7, 343, 868
BshFI GGCC 2 cut(s) 373, 1793
BshNI GGYRCC 6 cut(s) 1075, 1114, 1294, 1327, 1510, 1540
BshTI ACCGGT 6 cut(s) 1063, 1132, 1330, 1483, 1507, 1543
BsiHKAI GWGCWC 1 cut(s) 1669
BsiHKCI CYCGRG 1 cut(s) 1705
BslFI GGGAC 3 cut(s) 305, 1579, 1765
BslI CCNNNNNNNGG 4 cut(s) 575, 689, 702, 1132
BsmAI GTCTC 4 cut(s) 406, 762, 971, 1095
BsmBI CGTCTC 2 cut(s) 971, 1095
BsmFI GGGAC 3 cut(s) 305, 1579, 1765
BsnI GGCC 2 cut(s) 373, 1793
Bso31I GGTCTC 2 cut(s) 406, 762
BsoBI CYCGRG 1 cut(s) 1705
Bsp1286I GDGCHC 3 cut(s) 16, 1543, 1669
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 7 cut(s) 68, 1013, 1166, 1214, 1247, 1595, 1743
BspANI GGCC 2 cut(s) 373, 1793
BspFNI CGCG 3 cut(s) 7, 343, 868
BspPI GGATC 9 cut(s) 152, 165, 288, 687, 700, 768, 781, 1261, 1366
BspT107I GGYRCC 6 cut(s) 1075, 1114, 1294, 1327, 1510, 1540
BspTNI GGTCTC 2 cut(s) 406, 762
BsrDI GCAATG 1 cut(s) 986
BsrI ACTGG 7 cut(s) 534, 655, 1008, 1301, 1364, 1440, 1464
BssHII GCGCGC 1 cut(s) 341
BssNI GRCGYC 1 cut(s) 980
BssSI CACGAG 2 cut(s) 1579, 1608
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 2 cut(s) 1579, 1608
Bst2UI CCWGG 3 cut(s) 183, 375, 1563
Bst4CI ACNGT 3 cut(s) 85, 104, 113
Bst6I CTCTTC 2 cut(s) 592, 1723
BstACI GRCGYC 1 cut(s) 980
BstC8I GCNNGC 4 cut(s) 343, 1218, 1251, 1758
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 7 cut(s) 683, 1197, 1230, 1406, 1415, 1535, 1631
BstENI CCTNNNNNAGG 2 cut(s) 573, 700
BstF5I GGATG 4 cut(s) 845, 1068, 1489, 1712
BstFNI CGCG 3 cut(s) 7, 343, 868
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
BstMAI GTCTC 4 cut(s) 406, 762, 971, 1095
BstMWI GCNNNNNNNGC 4 cut(s) 11, 988, 1367, 1430
BstNI CCWGG 3 cut(s) 183, 375, 1563
BstSCI CCNGG 5 cut(s) 181, 373, 1071, 1107, 1561
BstSFI CTRYAG 2 cut(s) 249, 570
BstSLI GKGCMC 1 cut(s) 1543
BstUI CGCG 3 cut(s) 7, 343, 868
BstV2I GAAGAC 1 cut(s) 685
BstX2I RGATCY 3 cut(s) 157, 692, 773
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 3 cut(s) 157, 692, 773
BsuI GTATCC 2 cut(s) 445, 1000
BsuRI GGCC 2 cut(s) 373, 1793
BtgI CCRYGG 7 cut(s) 683, 1197, 1230, 1406, 1415, 1535, 1631
BtsCI GGATG 4 cut(s) 845, 1068, 1489, 1712
BtsIMutI CAGTG 5 cut(s) 883, 1308, 1371, 1433, 1457
Cac8I GCNNGC 4 cut(s) 343, 1218, 1251, 1758
CfoI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 4 cut(s) 594, 945, 988, 1762
Csp6I GTAC 5 cut(s) 243, 1001, 1115, 1487, 1511
CspAI ACCGGT 6 cut(s) 1063, 1132, 1330, 1483, 1507, 1543
CviAII CATG 6 cut(s) 317, 684, 727, 1036, 1654, 1690
CviQI GTAC 5 cut(s) 243, 1001, 1115, 1487, 1511
DdeI CTNAG 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 592, 1723
EarI CTCTTC 2 cut(s) 592, 1723
EciI GGCGGA 2 cut(s) 1203, 1236
Ecl136II GAGCTC 1 cut(s) 1667
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 16, 1669
Eco31I GGTCTC 2 cut(s) 406, 762
Eco53kI GAGCTC 1 cut(s) 1667
Eco88I CYCGRG 1 cut(s) 1705
EcoICRI GAGCTC 1 cut(s) 1667
EcoNI CCTNNNNNAGG 2 cut(s) 573, 700
EcoRII CCWGG 3 cut(s) 181, 373, 1561
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 16, 1669
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 2 cut(s) 971, 1095
FaeI CATG 6 cut(s) 320, 687, 730, 1039, 1657, 1693
FaqI GGGAC 3 cut(s) 305, 1579, 1765
FatI CATG 6 cut(s) 316, 683, 726, 1035, 1653, 1689
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 941
Fnu4HI GCNGC 1 cut(s) 1744
FokI GGATG 4 cut(s) 832, 1055, 1476, 1699
FriOI GRGCYC 2 cut(s) 16, 1669
Fsp4HI GCNGC 1 cut(s) 1744
FspBI CTAG 4 cut(s) 17, 702, 749, 833
GlaI GCGC 7 cut(s) 6, 342, 344, 393, 532, 591, 867
GluI GCNGC 1 cut(s) 1744
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 2 cut(s) 373, 1793
HgaI GACGC 4 cut(s) 594, 945, 988, 1762
HhaI GCGC 7 cut(s) 7, 343, 345, 394, 533, 592, 868
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 6 cut(s) 320, 687, 730, 1039, 1657, 1693
Hin6I GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
HinP1I GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 6 cut(s) 134, 1138, 1171, 1306, 1339, 1519
Hpy166II GTNNAC 4 cut(s) 609, 812, 942, 1679
Hpy188I TCNGA 3 cut(s) 285, 331, 769
Hpy188III TCNNGA 3 cut(s) 215, 449, 1670
Hpy8I GTNNAC 4 cut(s) 609, 812, 942, 1679
Hpy99I CGWCG 8 cut(s) 830, 961, 1276, 1279, 1351, 1381, 1384, 1450
HpyAV CCTTC 2 cut(s) 497, 1667
HpyCH4III ACNGT 3 cut(s) 85, 104, 113
HpyCH4IV ACGT 2 cut(s) 275, 1448
HpyCH4V TGCA 3 cut(s) 308, 893, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 988, 1367, 1430
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 275, 1448
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 6 cut(s) 320, 687, 730, 1039, 1657, 1693
HspAI GCGC 7 cut(s) 5, 341, 343, 392, 531, 590, 866
KpnI GGTACC 2 cut(s) 1118, 1514
Ksp22I TGATCA 1 cut(s) 88
LmnI GCTCC 1 cut(s) 836
LweI GCATC 2 cut(s) 115, 1721
MaeI CTAG 4 cut(s) 17, 702, 749, 833
MaeII ACGT 2 cut(s) 275, 1448
MaeIII GTNAC 6 cut(s) 52, 98, 494, 554, 949, 1399
MboII GAAGA 5 cut(s) 609, 690, 743, 1546, 1710
MflI RGATCY 3 cut(s) 157, 692, 773
MhlI GDGCHC 3 cut(s) 16, 1543, 1669
MmeI TCCRAC 2 cut(s) 354, 873
MroXI GAANNNNTTC 1 cut(s) 1736
MseI TTAA 6 cut(s) 236, 440, 636, 791, 858, 1728
MslI CAYNNNNRTG 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 1743
MspR9I CCNGG 5 cut(s) 183, 375, 1073, 1109, 1563
MvaI CCWGG 3 cut(s) 183, 375, 1563
MvnI CGCG 3 cut(s) 7, 343, 868
MwoI GCNNNNNNNGC 4 cut(s) 11, 988, 1367, 1430
NciI CCSGG 2 cut(s) 1073, 1109
NcoI CCATGG 1 cut(s) 683
NlaIII CATG 6 cut(s) 320, 687, 730, 1039, 1657, 1693
NmeAIII GCCGAG 1 cut(s) 1748
NmuCI GTSAC 3 cut(s) 52, 554, 1399
PauI GCGCGC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 1283, 1388
PdmI GAANNNNTTC 1 cut(s) 1736
PfeI GAWTC 6 cut(s) 134, 1138, 1171, 1306, 1339, 1519
PflFI GACNNNGTC 1 cut(s) 982
PinAI ACCGGT 6 cut(s) 1063, 1132, 1330, 1483, 1507, 1543
PkrI GCNGC 1 cut(s) 1745
PshBI ATTAAT 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1625
Psp124BI GAGCTC 1 cut(s) 1669
Psp6I CCWGG 3 cut(s) 181, 373, 1561
PspGI CCWGG 3 cut(s) 181, 373, 1561
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 3 cut(s) 157, 692, 773
PsyI GACNNNGTC 1 cut(s) 982
PteI GCGCGC 1 cut(s) 341
RsaI GTAC 5 cut(s) 244, 1002, 1116, 1488, 1512
RsaNI GTAC 5 cut(s) 243, 1001, 1115, 1487, 1511
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 1 cut(s) 1669
SaqAI TTAA 6 cut(s) 236, 440, 636, 791, 858, 1728
SatI GCNGC 1 cut(s) 1744
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 5 cut(s) 183, 375, 1073, 1109, 1563
SduI GDGCHC 3 cut(s) 16, 1543, 1669
SfaNI GCATC 2 cut(s) 115, 1721
SfcI CTRYAG 2 cut(s) 249, 570
SgrAI CRCCGGYG 4 cut(s) 1132, 1162, 1330, 1543
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 2 cut(s) 63, 1668
SmoI CTYRAG 2 cut(s) 63, 1668
SsiI CCGC 7 cut(s) 68, 1013, 1166, 1214, 1247, 1595, 1743
SspMI CTAG 4 cut(s) 17, 702, 749, 833
SstI GAGCTC 1 cut(s) 1669
StyD4I CCNGG 5 cut(s) 181, 373, 1071, 1107, 1561
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 3 cut(s) 85, 104, 113
TaiI ACGT 2 cut(s) 278, 1451
TaqI TCGA 2 cut(s) 173, 1391
TauI GCSGC 1 cut(s) 1746
TfiI GAWTC 6 cut(s) 134, 1138, 1171, 1306, 1339, 1519
Tru1I TTAA 6 cut(s) 236, 440, 636, 791, 858, 1728
Tru9I TTAA 6 cut(s) 236, 440, 636, 791, 858, 1728
TscAI CASTG 5 cut(s) 883, 1308, 1371, 1440, 1464
TseFI GTSAC 3 cut(s) 52, 554, 1399
Tsp45I GTSAC 3 cut(s) 52, 554, 1399
TspDTI ATGAA 3 cut(s) 217, 305, 1052
TspGWI ACGGA 4 cut(s) 843, 1299, 1512, 1620
TspRI CASTG 5 cut(s) 883, 1308, 1371, 1440, 1464
Tth111I GACNNNGTC 1 cut(s) 982
VspI ATTAAT 1 cut(s) 791
XagI CCTNNNNNAGG 2 cut(s) 573, 700
XapI RAATTY 2 cut(s) 872, 895
XmiI GTMKAC 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 1736
XspI CTAG 4 cut(s) 17, 702, 749, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.