Rh5AG433500

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003654
Physical Location & Seq
Reverse (-)
74545091 .. 74546456
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG433500.1

Sequence Viewer

Length: 1014 bp
ATGATTCGTGGTACTTTTATGATGAACTTGGTATTCATAGCTTTGTATGCTTTCATGGCTATTTCAAATTCTCAAGCCGAAGGAACAAACACGAGTACTTCAGCGATTCTCATAACAGTTGACCAATCGGGCAAAGGTGATTACAAGAAAATACAAGAAGCCATTGATTCAGTGGCGTCCAACAACGCAGAGCTCGTGTTTATTAGGGTTAAGCCCGGAGTATATGAAGAAAAAGTTATGGTTCCGGCGGACAAGCCGTTTATAACAATAAGTGGCACAAAGGCAGCTGACACGGTTATAACATGGAACGATAGTGGGGACATATTCGAGTCTCCAACTTTCTCTGTCCTGGCCTCTGATTTCATAGGGCGTTACCTCACAATTCAGAATACATATGGAGCTGGTGTGAAAGCGGTGGCATTACGGGTGTCTGGAGATAGAGCAGCATTCTATGGGTGCAGAATTTTGTCTCATCAAGACACCCTCCTAGATGATACTGGGAGGCACTACTACAACAACTGTTACATTGAAGGAGATGTAGACTTCATATGTGGAAATGCTGCTTCTTTCTTTGAGAAGTGCCATTTGCATTCTCTTTCGGTAGGAACCGGGGCCATAACGGCCCAACGGAGAATGTCCCCGGCTGAGGACACCGGGTTCATGTTCTTCGGGTGCAAGATCACCGGTGTCAAAACAGCAGTGTTGGGAAGGCCTTGGGGTACATATTCAAGAGTGGTCTTTGTCTTCACTTACATGACTAGCGCCATACTGCCACAAGGTTGGGACGATTGGGGAAACTCAGCCGACCAATTAAGAACCGTATACTATGGACAGTATAAATGTTCTGGACGGGGAGCAACTACATCGAAACGAGTAGAATGGGCACGCGACCTTACGAGCGAAGAAGTCGAACCCTTCTTGACTAAGGACATGGTTGATGCCAATGCTTGGTTAAGGTCAGCACCAACCAGTTTCAAGAAGAAAGCACTCAGTGCCAATGACACCAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

337

Amino Acids

37.1

Weight (kDa)

6.44

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 38 - 313 5.9e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 263, 299
AccB7I CCANNNNNTGG 1 cut(s) 948
AccI GTMKAC 2 cut(s) 540, 822
AccII CGCG 1 cut(s) 888
AciI CCGC 2 cut(s) 248, 413
AcsI RAATTY 2 cut(s) 67, 462
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 992
AfaI GTAC 3 cut(s) 13, 97, 721
AfiI CCNNNNNNNGG 2 cut(s) 646, 948
AgeI ACCGGT 1 cut(s) 683
AgsI TTSAA 4 cut(s) 66, 530, 729, 976
AjnI CCWGG 1 cut(s) 348
AloI GAACNNNNNNTCC 2 cut(s) 641, 673
AluBI AGCT 4 cut(s) 41, 193, 287, 401
AluI AGCT 4 cut(s) 41, 193, 287, 401
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 336, 474
AoxI GGCC 4 cut(s) 351, 612, 621, 710
ApeKI GCWGC 3 cut(s) 284, 443, 560
ApoI RAATTY 2 cut(s) 67, 462
AsiGI ACCGGT 1 cut(s) 683
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 2 cut(s) 612, 622
AsuC2I CCSGG 4 cut(s) 216, 610, 641, 655
AsuHPI GGTGA 2 cut(s) 149, 673
BaeGI GKGCMC 1 cut(s) 886
BanII GRGCYC 1 cut(s) 195
BauI CACGAG 2 cut(s) 91, 194
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 296, 455, 547
BceAI ACGGC 2 cut(s) 241, 636
BcgI CGANNNNNNTGC 2 cut(s) 846, 880
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 4 cut(s) 216, 610, 641, 655
BcoDI GTCTC 2 cut(s) 336, 474
BfaI CTAG 2 cut(s) 488, 759
BfoI RGCGCY 1 cut(s) 765
BglI GCCNNNNNGGC 1 cut(s) 620
BisI GCNGC 3 cut(s) 285, 444, 561
BlsI GCNGC 3 cut(s) 286, 445, 562
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 5 cut(s) 216, 350, 610, 641, 655
BmgT120I GGNCC 2 cut(s) 612, 622
BmiI GGNNCC 3 cut(s) 243, 607, 613
BmrFI CCNGG 5 cut(s) 216, 350, 610, 641, 655
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 1 cut(s) 928
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 1 cut(s) 736
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 1 cut(s) 645
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 4 cut(s) 216, 610, 641, 655
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 609, 639, 713
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 948
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 2 cut(s) 502, 969
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 3 cut(s) 609, 639, 713
BseLI CCNNNNNNNGG 2 cut(s) 646, 948
BseMII CTCAG 3 cut(s) 636, 813, 1003
BseNI ACTGG 2 cut(s) 502, 969
BseSI GKGCMC 1 cut(s) 886
BseXI GCAGC 3 cut(s) 296, 455, 547
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 888
BshFI GGCC 4 cut(s) 353, 614, 623, 712
BshTI ACCGGT 1 cut(s) 683
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 6 cut(s) 216, 245, 609, 641, 654, 684
BslFI GGGAC 3 cut(s) 332, 622, 797
BslI CCNNNNNNNGG 2 cut(s) 646, 948
BsmAI GTCTC 2 cut(s) 336, 474
BsmFI GGGAC 3 cut(s) 332, 622, 797
BsmI GAATGC 2 cut(s) 446, 589
BsnI GGCC 4 cut(s) 353, 614, 623, 712
Bsp1286I GDGCHC 2 cut(s) 195, 886
Bsp143I GATC 1 cut(s) 678
BspACI CCGC 2 cut(s) 248, 413
BspANI GGCC 4 cut(s) 353, 614, 623, 712
BspCNI CTCAG 3 cut(s) 637, 812, 1002
BspFNI CGCG 1 cut(s) 888
BspLI GGNNCC 3 cut(s) 243, 607, 613
BsrFI RCCGGY 1 cut(s) 683
BsrI ACTGG 2 cut(s) 502, 969
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 3 cut(s) 609, 639, 713
BssMI GATC 1 cut(s) 678
BssNAI GTATAC 1 cut(s) 823
BssNI GRCGYC 1 cut(s) 176
BssSI CACGAG 2 cut(s) 91, 194
BssT1I CCWWGG 1 cut(s) 713
Bst1107I GTATAC 1 cut(s) 823
Bst2BI CACGAG 2 cut(s) 91, 194
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 5 cut(s) 118, 295, 521, 820, 834
BstACI GRCGYC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 992
BstC8I GCNNGC 1 cut(s) 886
BstDEI CTNAG 4 cut(s) 645, 799, 924, 989
BstFNI CGCG 1 cut(s) 888
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 1 cut(s) 681
BstMAI GTCTC 2 cut(s) 336, 474
BstMBI GATC 1 cut(s) 678
BstMWI GCNNNNNNNGC 4 cut(s) 47, 56, 620, 992
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 5 cut(s) 214, 348, 608, 639, 653
BstSLI GKGCMC 1 cut(s) 886
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 3 cut(s) 296, 455, 547
BstV2I GAAGAC 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 780
BstZ17I GTATAC 1 cut(s) 823
BsuRI GGCC 4 cut(s) 353, 614, 623, 712
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 177, 705, 997
Cac8I GCNNGC 1 cut(s) 886
CfoI GCGC 1 cut(s) 764
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 2 cut(s) 612, 622
CseI GACGC 1 cut(s) 165
Csp6I GTAC 3 cut(s) 12, 96, 720
CspAI ACCGGT 1 cut(s) 683
CviAII CATG 5 cut(s) 55, 303, 661, 754, 931
CviQI GTAC 3 cut(s) 12, 96, 720
DdeI CTNAG 4 cut(s) 645, 799, 924, 989
DpnI GATC 1 cut(s) 680
DpnII GATC 1 cut(s) 678
DraIII CACNNNGTG 1 cut(s) 992
EciI GGCGGA 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 713
Eco147I AGGCCT 1 cut(s) 712
Eco24I GRGCYC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 5 cut(s) 58, 306, 664, 757, 934
FaqI GGGAC 3 cut(s) 332, 622, 797
FatI CATG 5 cut(s) 54, 302, 660, 753, 930
FauNDI CATATG 2 cut(s) 394, 548
FblI GTMKAC 2 cut(s) 540, 822
Fnu4HI GCNGC 3 cut(s) 285, 444, 561
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 285, 444, 561
FspBI CTAG 2 cut(s) 488, 759
GlaI GCGC 1 cut(s) 763
GluI GCNGC 3 cut(s) 285, 444, 561
GsuI CTGGAG 1 cut(s) 453
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 4 cut(s) 353, 614, 623, 712
HapII CCGG 6 cut(s) 216, 245, 609, 641, 654, 684
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 764
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 5 cut(s) 58, 306, 664, 757, 934
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 4 cut(s) 4, 106, 167, 329
HpaII CCGG 6 cut(s) 216, 245, 609, 641, 654, 684
HphI GGTGA 2 cut(s) 149, 673
Hpy166II GTNNAC 3 cut(s) 121, 541, 823
Hpy188I TCNGA 2 cut(s) 358, 387
Hpy188III TCNNGA 6 cut(s) 432, 476, 729, 846, 919, 976
Hpy8I GTNNAC 3 cut(s) 121, 541, 823
HpyAV CCTTC 4 cut(s) 74, 524, 702, 925
HpyCH4III ACNGT 5 cut(s) 118, 295, 521, 820, 834
HpyCH4V TGCA 3 cut(s) 459, 589, 675
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 56, 620, 992
HpyF3I CTNAG 4 cut(s) 645, 799, 924, 989
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 5 cut(s) 58, 306, 664, 757, 934
HspAI GCGC 1 cut(s) 762
Kzo9I GATC 1 cut(s) 678
LmnI GCTCC 2 cut(s) 398, 854
Lsp1109I GCAGC 3 cut(s) 296, 455, 547
LweI GCATC 1 cut(s) 928
MaeI CTAG 2 cut(s) 488, 759
MaeIII GTNAC 2 cut(s) 371, 521
MalI GATC 1 cut(s) 680
MboI GATC 1 cut(s) 678
MboII GAAGA 5 cut(s) 239, 658, 736, 914, 991
MhlI GDGCHC 2 cut(s) 195, 886
MluCI AATT 5 cut(s) 67, 381, 462, 809, 1009
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 2 cut(s) 204, 359
MnlI CCTC 5 cut(s) 364, 386, 494, 495, 640
MseI TTAA 4 cut(s) 210, 812, 953, 1012
MslI CAYNNNNRTG 1 cut(s) 752
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 6 cut(s) 216, 245, 609, 641, 654, 684
MspR9I CCNGG 5 cut(s) 216, 350, 610, 641, 655
Mva1269I GAATGC 2 cut(s) 446, 589
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 4 cut(s) 47, 56, 620, 992
NciI CCSGG 4 cut(s) 216, 610, 641, 655
NdeI CATATG 2 cut(s) 394, 548
NdeII GATC 1 cut(s) 678
NlaIII CATG 5 cut(s) 58, 306, 664, 757, 934
NlaIV GGNNCC 3 cut(s) 243, 607, 613
PceI AGGCCT 1 cut(s) 712
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 2 cut(s) 446, 589
PfeI GAWTC 3 cut(s) 4, 106, 167
PflMI CCANNNNNTGG 1 cut(s) 948
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 3 cut(s) 286, 445, 562
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PsiI TTATAA 2 cut(s) 263, 299
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 3 cut(s) 243, 607, 613
PspPI GGNCC 2 cut(s) 612, 622
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 3 cut(s) 13, 97, 721
RsaNI GTAC 3 cut(s) 12, 96, 720
RseI CAYNNNNRTG 1 cut(s) 752
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 4 cut(s) 210, 812, 953, 1012
SatI GCNGC 3 cut(s) 285, 444, 561
Sau3AI GATC 1 cut(s) 678
Sau96I GGNCC 2 cut(s) 612, 622
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 5 cut(s) 216, 350, 610, 641, 655
SduI GDGCHC 2 cut(s) 195, 886
SetI ASST 9 cut(s) 43, 139, 195, 289, 378, 403, 781, 894, 959
SfaNI GCATC 1 cut(s) 928
SfiI GGCCNNNNNGGCC 1 cut(s) 620
SgrAI CRCCGGYG 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 5 cut(s) 67, 381, 462, 809, 1009
SseBI AGGCCT 1 cut(s) 712
SsiI CCGC 2 cut(s) 248, 413
SspMI CTAG 2 cut(s) 488, 759
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 712
StyD4I CCNGG 5 cut(s) 214, 348, 608, 639, 653
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 5 cut(s) 118, 295, 521, 820, 834
TaqI TCGA 3 cut(s) 327, 866, 909
TasI AATT 5 cut(s) 67, 381, 462, 809, 1009
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 3 cut(s) 4, 106, 167
Tru1I TTAA 4 cut(s) 210, 812, 953, 1012
Tru9I TTAA 4 cut(s) 210, 812, 953, 1012
TscAI CASTG 3 cut(s) 177, 705, 997
TseI GCWGC 3 cut(s) 284, 443, 560
TspDTI ATGAA 7 cut(s) 25, 38, 43, 240, 352, 535, 649
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 3 cut(s) 177, 705, 997
Van91I CCANNNNNTGG 1 cut(s) 948
XapI RAATTY 2 cut(s) 67, 462
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 540, 822
XspI CTAG 2 cut(s) 488, 759
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.