AT2G27210

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002717
Physical Location & Seq
Forward (+)
11629777 .. 11636803
7027 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27210.1

Sequence Viewer

Length: 3021 bp
ATGGATTTGGATTCTTCAATGGTACCAGAGAATGATCAAGATCCTATTGCTACGTCTGAGAATCAATCTCCTATGGAGGAGAAAGAAGAAGCGTCTGAGCAACAAACCGGATCCGAATCCGAATCGGCGTCTTTGACTCCTTCTCTTCCTCCTCCTTCGCAGCAACAACAACAACAGCAGCAACAGCCGCAGGTAACGGCGGTGGTTGGTCCGAGATGTGCGCCAACGTATTCGGTTGTGAATGCTATAATCGAGAAGAAAGAAGATGGTCCTGGTCCTAGATGTGGACATACGCTGACGGCGGTTCCTGCTGTTGGTGAAGAAGGGACGTCTTCTTATATTGGCCCGAGACTGATCTTGTTTGGTGGTGCTACTGCTCTTGAGGGTAATTCTGGAGGAACGGGAACACCTACTTCAGCTGGAAGTGCTGGCATTCGGTTAGCTGGTGCAACCGCTGATGTTCATTGTTATGATGTTCTTTCTAATAAGTGGTCGAGGCTTACACCTTATGGAGAACCACCTAGTCCAAGAGCTGCTCATGTTGCTACTGCTGTTGGGACCATGGTAGTAATTCAGGGGGGAATAGGTCCTGCTGGTTTATCAGCTGAGGATCTTCACGTTCTTGATCTCACACAGCAACGGCCACGATGGCACAGGGTTGTTGTTCAGGGTCCTGGACCAGGACCACGTTATGGACATGTCATGGCACTGGTAGGACAGAGGTATCTCATGGCAATTGGTGGAAATGATGGGAAACGTCCATTAGCTGATGTATGGGCTTTGGACACTGCTGCCAAACCTTATGAATGGCGTAAGCTGGAACCGGAAGGGGAAGGTCCACCACCATGCATGTATGCAACTGCAAGTGCGCGATCTGATGGCCTTCTTCTTCTCTGTGGTGGAAGAGATGCGAATAGTGTGCCCCTAGCAAGTGCATATGGACTTGCCAAGCACAGAGATGGACGTTGGGAATGGGCCATAGCCCCAGGTGTCTCCCCTTCTGCCAGATACCAGCATGCTGCAGTCTTTGTAAATGCAAGACTCCATGTCTCTGGTGGGGCACTTGGTGGTGGACGCATGGTAGAAGACTCGTCCAGTGTTGCAGTGTTGGATACTGCAGCAGGTGTTTGGTGTGATACAAAATCGGTTGTTACTAGTCCTAGAACTGGTAGGTATAGTGCTGACGCAGCTGGTGGTGATGCTTCTGTTGAGCTGACTAGGCGATGCAGGCATGCTGCTGCTGCAGTGGGTGATTTGATATTCATCTATGGTGGGTTGCGTGGAGGAGTTTTACTTGATGACCTATTGGTTGCTGAAGATCTTGCTGCAGCGGAAACAACATCTGCTGCCTCTCATGCTGCTGCTGCAGCAGCAGCAACAAACACGCCACCTGGCCGTTCACCTGGAAGATATGGATTTTCTGATGAAAGGACAGGGGAGCTACCAGAATCAGCTCCTGACGCTGTGGTGCTGGGAAGCCCTGTTGCACCCCCTGTAAATGGTGATATGTATACTGATATTAGCACTGAAAATGCAATGGTTCCAGGAATTCGGAGAACAAGCAAGGGTGTGGAGTATCTTGTTGAAGCATCTGCTGCGGAAGCCGAGGCTATCAGTGCAACATTAGCTGCTGCGAAGGCACGACAGGTCAATGGGGAAGTTGAACTTCCAGATAGGGATCGTGGTGCTGAGGCTACGCCCAGTGGAAAACCTTCATTATCATTGATTAAGCCTGATTCTGCAGTACCAAATAGTGTTATTCCTGCAGGGGTTCGACTTCATCATAGAGCTGTGGTGGTTGCTGCAGAAACAGGTGGAGCCTTAGGTGGAATGGTTAGACAGTTATCCATTGATCAATTTGAAAATGAGGGACGCCGTGTTAGTTATGGGACACCCGAAAGTGCAACAGCAGCTAGGAAATTATTAGATCGTCAGATGTCAATCAATAGTGTTCCGAAAAAGGTTGTAGCTCATCTTTTGAAGCCTCGGGGGTGGAAGCCTCCAGTTCGTCGCCAGTTTTTCTTGGATTGCAATGAAATAGCTGATCTTTGCGACAGTGCAGAACGTATCTTCTCAAGCGAACCTACTGTGTTACAGCTTAAAGCTCCTATTAAGATATTTGGTGATTTGCATGGCCAGTTTGGGGATCTCATGCGCCTTTTTGATGAATATGGTTCACCATCAACAGCTGGAGACATATCATACATCGATTACCTCTTCTTAGGAGACTATGTTGATCGGGGTCAACACAGCCTAGAGACAATTACACTTCTGCTTGCTTTAAAGGTTGAATACCAACATAACGTACACCTGATTCGTGGAAATCATGAAGCTGCCGACATTAATGCACTTTTTGGTTTCCGGATAGAGTGTATTGAGCGAATGGGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGGCTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGTGGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGATTTATTATGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTAAGGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGGCCTGACCGTGTCATGGAGTTTTGCAACAACAATGATCTGCAATTGATTGTACGTGCGCATGAATGCGTGATGGACGGATTTGAGCGTTTTGCCCAAGGTCATTTGATAACGCTCTTTTCGGCAACAAATTATTGTGGTACCGCAAACAATGCCGGGGCTATCTTGGTGTTGGGGAGAGATCTTGTGGTGGTTCCTAAGCTTATCCATCCATTGCCTCCAGCGATAACCTCACCTGAAACTTCACCAGAAAGACATATAGAGGATACATGGATGCAGGAGTTAAACGTAAATAGACCACCAACACCAACAAGAGGACGCCCACAAAATCCAAATGACCGCGGCTCTCTTGCTTGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_2 Kelch_3 Kelch_4 Metallophos

Protein Analysis

1006

Amino Acids

107.52

Weight (kDa)

5.48

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 87 - 300 1.4e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 89 - 264 4.2e-22 FBX42, beta-propeller domain
Kelch_HCF PF13854 149 - 427 2.2e-10 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 252 - 435 8.2e-13 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 269 - 391 2.6e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 336 - 382 1.4e-06 Kelch motif
Metallophos PF00149 703 - 910 5.1e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1112
AatII GACGTC 1 cut(s) 332
Acc16I TGCGCA 1 cut(s) 2718
Acc36I ACCTGC 2 cut(s) 181, 1112
Acc65I GGTACC 2 cut(s) 22, 2798
AccB1I GGYRCC 2 cut(s) 22, 2798
AccB7I CCANNNNNTGG 1 cut(s) 692
AccI GTMKAC 1 cut(s) 1511
AccII CGCG 2 cut(s) 871, 3000
AccIII TCCGGA 1 cut(s) 2361
AciI CCGC 9 cut(s) 188, 200, 302, 453, 1331, 1598, 2802, 2998, 3000
AclWI GGATC 9 cut(s) 35, 105, 118, 618, 1686, 2154, 2408, 2585, 2598
AcoI YGGCCR 3 cut(s) 641, 1393, 2134
AcsI RAATTY 1 cut(s) 1548
AcuI CTGAAG 2 cut(s) 399, 1335
AcyI GRCGYC 4 cut(s) 128, 329, 1873, 2977
AdeI CACNNNGTG 1 cut(s) 1067
AfaI GTAC 6 cut(s) 24, 1746, 2307, 2568, 2712, 2800
AflIII ACRYGT 1 cut(s) 697
AgsI TTSAA 7 cut(s) 18, 1586, 1664, 1862, 1981, 2291, 2434
AhdI GACNNNNNGTC 1 cut(s) 1046
AhlI ACTAGT 1 cut(s) 1154
AjnI CCWGG 8 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641
Alw26I GTCTC 6 cut(s) 343, 997, 1054, 2187, 2220, 2252
AlwI GGATC 9 cut(s) 35, 105, 118, 618, 1686, 2154, 2408, 2585, 2598
AlwNI CAGNNNCTG 1 cut(s) 1457
Ama87I CYCGRG 2 cut(s) 346, 1986
Aor13HI TCCGGA 1 cut(s) 2361
AoxI GGCC 9 cut(s) 343, 641, 880, 975, 1393, 2134, 2624, 2638, 2660
ApoI RAATTY 1 cut(s) 1548
AseI ATTAAT 1 cut(s) 2343
Asp700I GAANNNNTTC 1 cut(s) 1711
Asp718I GGTACC 2 cut(s) 22, 2798
AspLEI GCGC 4 cut(s) 223, 871, 2157, 2719
AsuC2I CCSGG 1 cut(s) 2815
AvaI CYCGRG 2 cut(s) 346, 1986
AvaII GGWCC 9 cut(s) 209, 269, 275, 558, 587, 671, 677, 683, 836
AxyI CCTNAGG 1 cut(s) 1822
BaeGI GKGCMC 2 cut(s) 924, 1063
BaeI ACNNNNGTAYC 2 cut(s) 707, 740
BalI TGGCCA 1 cut(s) 2136
BamHI GGATCC 2 cut(s) 110, 2590
BanI GGYRCC 2 cut(s) 22, 2798
BbsI GAAGAC 2 cut(s) 324, 1092
BbvCI CCTCAGC 2 cut(s) 606, 1689
BceAI ACGGC 5 cut(s) 213, 315, 656, 1380, 1860
BcgI CGANNNNNNTGC 4 cut(s) 901, 935, 2327, 2361
BciT130I CCWGG 8 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643
BciVI GTATCC 2 cut(s) 1105, 2917
BclI TGATCA 2 cut(s) 34, 1852
BcnI CCSGG 1 cut(s) 2815
BcoDI GTCTC 6 cut(s) 343, 997, 1054, 2187, 2220, 2252
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 9 cut(s) 279, 522, 926, 1155, 1161, 1218, 1914, 2255, 2634
BfmI CTRYAG 8 cut(s) 1020, 1116, 1242, 1326, 1365, 1740, 1764, 1803
BfuAI ACCTGC 2 cut(s) 181, 1112
BfuI GTATCC 2 cut(s) 1105, 2917
BglI GCCNNNNNGGC 1 cut(s) 649
BglII AGATCT 2 cut(s) 1318, 2839
Bme1390I CCNGG 9 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643, 2815
Bme18I GGWCC 9 cut(s) 209, 269, 275, 558, 587, 671, 677, 683, 836
BmeRI GACNNNNNGTC 1 cut(s) 1046
BmeT110I CYCGRG 2 cut(s) 346, 1986
BmrFI CCNGG 9 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643, 2815
BmrI ACTGGG 1 cut(s) 1695
BmsI GCATC 6 cut(s) 898, 1189, 1214, 1598, 2422, 2922
BmuI ACTGGG 1 cut(s) 1695
BpiI GAAGAC 2 cut(s) 324, 1092
BpmI CTGGAG 4 cut(s) 414, 1986, 2211, 2862
Bpu10I CCTNAGC 3 cut(s) 606, 1689, 2856
BpuEI CTTGAG 2 cut(s) 401, 2059
BpuMI CCSGG 1 cut(s) 2815
Bsa29I ATCGAT 1 cut(s) 2208
BsaAI YACGTR 1 cut(s) 2714
BsaBI GATNNNNATC 5 cut(s) 39, 115, 1262, 1677, 1940
BsaHI GRCGYC 4 cut(s) 128, 329, 1873, 2977
BsaJI CCNNGG 9 cut(s) 561, 985, 1605, 1985, 2641, 2642, 2755, 2814, 2998
BsaWI WCCGGW 3 cut(s) 107, 823, 2361
BsaXI ACNNNNNCTCC 2 cut(s) 2217, 2247
Bse1I ACTGG 7 cut(s) 714, 1095, 1171, 1701, 2003, 2014, 2137
Bse21I CCTNAGG 1 cut(s) 1822
Bse3DI GCAATG 3 cut(s) 1542, 2038, 2870
Bse8I GATNNNNATC 5 cut(s) 39, 115, 1262, 1677, 1940
BseAI TCCGGA 1 cut(s) 2361
BseBI CCWGG 8 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643
BseCI ATCGAT 1 cut(s) 2208
BseDI CCNNGG 9 cut(s) 561, 985, 1605, 1985, 2641, 2642, 2755, 2814, 2998
BseGI GGATG 2 cut(s) 2866, 2937
BseJI GATNNNNATC 5 cut(s) 39, 115, 1262, 1677, 1940
BseMI GCAATG 3 cut(s) 1542, 2038, 2870
BseMII CTCAG 4 cut(s) 48, 87, 597, 1680
BseNI ACTGG 7 cut(s) 714, 1095, 1171, 1701, 2003, 2014, 2137
BseRI GAGGAG 3 cut(s) 92, 141, 1299
BseSI GKGCMC 2 cut(s) 924, 1063
BseYI CCCAGC 1 cut(s) 1471
BsgI GTGCAG 2 cut(s) 2079, 2436
Bsh1236I CGCG 2 cut(s) 871, 3000
Bsh1285I CGRYCG 1 cut(s) 2501
BshFI GGCC 9 cut(s) 345, 643, 882, 977, 1395, 2136, 2626, 2640, 2662
BshNI GGYRCC 2 cut(s) 22, 2798
BshVI ATCGAT 1 cut(s) 2208
BsiEI CGRYCG 1 cut(s) 2501
BsiHKCI CYCGRG 2 cut(s) 346, 1986
BsiSI CCGG 4 cut(s) 108, 824, 2362, 2814
BslFI GGGAC 4 cut(s) 340, 571, 1884, 1903
BsmAI GTCTC 6 cut(s) 343, 997, 1054, 2187, 2220, 2252
BsmFI GGGAC 4 cut(s) 340, 571, 1884, 1903
BsmI GAATGC 3 cut(s) 247, 432, 2729
BsnI GGCC 9 cut(s) 345, 643, 882, 977, 1395, 2136, 2626, 2640, 2662
BsoBI CYCGRG 2 cut(s) 346, 1986
Bsp1286I GDGCHC 2 cut(s) 924, 1063
Bsp13I TCCGGA 1 cut(s) 2361
Bsp19I CCATGG 1 cut(s) 561
BspACI CCGC 9 cut(s) 188, 200, 302, 453, 1331, 1598, 2802, 2998, 3000
BspANI GGCC 9 cut(s) 345, 643, 882, 977, 1395, 2136, 2626, 2640, 2662
BspCNI CTCAG 4 cut(s) 49, 88, 598, 1681
BspDI ATCGAT 1 cut(s) 2208
BspEI TCCGGA 1 cut(s) 2361
BspFNI CGCG 2 cut(s) 871, 3000
BspHI TCATGA 1 cut(s) 2326
BspMAI CTGCAG 8 cut(s) 1024, 1120, 1246, 1330, 1369, 1744, 1768, 1807
BspMI ACCTGC 2 cut(s) 181, 1112
BspPI GGATC 9 cut(s) 35, 105, 118, 618, 1686, 2154, 2408, 2585, 2598
BspT107I GGYRCC 2 cut(s) 22, 2798
BsrDI GCAATG 3 cut(s) 1542, 2038, 2870
BsrI ACTGG 7 cut(s) 714, 1095, 1171, 1701, 2003, 2014, 2137
BssECI CCNNGG 9 cut(s) 561, 985, 1605, 1985, 2641, 2642, 2755, 2814, 2998
BssNAI GTATAC 1 cut(s) 1512
BssNI GRCGYC 4 cut(s) 128, 329, 1873, 2977
BssT1I CCWWGG 2 cut(s) 561, 2755
Bst1107I GTATAC 1 cut(s) 1512
Bst2UI CCWGG 8 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643
Bst4CI ACNGT 6 cut(s) 1842, 2057, 2089, 2426, 2609, 2669
Bst6I CTCTTC 3 cut(s) 150, 898, 2222
BstACI GRCGYC 4 cut(s) 128, 329, 1873, 2977
BstAPI GCANNNNNTGC 2 cut(s) 1595, 2810
BstBAI YACGTR 1 cut(s) 2714
BstC8I GCNNGC 6 cut(s) 430, 1017, 1229, 1233, 2277, 2557
BstDEI CTNAG 7 cut(s) 57, 96, 606, 1689, 1822, 2221, 2856
BstDSI CCRYGG 2 cut(s) 561, 2998
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 2 cut(s) 2866, 2937
BstFNI CGCG 2 cut(s) 871, 3000
BstHHI GCGC 4 cut(s) 223, 871, 2157, 2719
BstMAI GTCTC 6 cut(s) 343, 997, 1054, 2187, 2220, 2252
BstMCI CGRYCG 1 cut(s) 2501
BstNI CCWGG 8 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643
BstNSI RCATGY 4 cut(s) 701, 853, 1019, 1235
BstSCI CCNGG 9 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641, 2813
BstSFI CTRYAG 8 cut(s) 1020, 1116, 1242, 1326, 1365, 1740, 1764, 1803
BstSLI GKGCMC 2 cut(s) 924, 1063
BstUI CGCG 2 cut(s) 871, 3000
BstV2I GAAGAC 2 cut(s) 324, 1092
BstX2I RGATCY 8 cut(s) 40, 110, 610, 1318, 2146, 2400, 2590, 2839
BstXI CCANNNNNNTGG 1 cut(s) 1052
BstYI RGATCY 8 cut(s) 40, 110, 610, 1318, 2146, 2400, 2590, 2839
BstZ17I GTATAC 1 cut(s) 1512
Bsu15I ATCGAT 1 cut(s) 2208
Bsu36I CCTNAGG 1 cut(s) 1822
BsuI GTATCC 2 cut(s) 1105, 2917
BsuRI GGCC 9 cut(s) 345, 643, 882, 977, 1395, 2136, 2626, 2640, 2662
BsuTUI ATCGAT 1 cut(s) 2208
BtgI CCRYGG 2 cut(s) 561, 2998
BtgZI GCGATG 1 cut(s) 1237
BtsCI GGATG 2 cut(s) 2866, 2937
BtsI GCAGTG 3 cut(s) 786, 1110, 1251
BveI ACCTGC 2 cut(s) 181, 1112
Cac8I GCNNGC 6 cut(s) 430, 1017, 1229, 1233, 2277, 2557
CaiI CAGNNNCTG 1 cut(s) 1457
CciI TCATGA 1 cut(s) 2326
CfoI GCGC 4 cut(s) 223, 871, 2157, 2719
Cfr42I CCGCGG 1 cut(s) 3001
ClaI ATCGAT 1 cut(s) 2208
CseI GACGC 7 cut(s) 81, 117, 1083, 1193, 1469, 1881, 2985
Csp6I GTAC 6 cut(s) 23, 1745, 2306, 2567, 2711, 2799
CviQI GTAC 6 cut(s) 23, 1745, 2306, 2567, 2711, 2799
DdeI CTNAG 7 cut(s) 57, 96, 606, 1689, 1822, 2221, 2856
DraI TTTAAA 1 cut(s) 2283
DraIII CACNNNGTG 1 cut(s) 1067
DriI GACNNNNNGTC 1 cut(s) 1046
EaeI YGGCCR 3 cut(s) 641, 1393, 2134
Eam1104I CTCTTC 3 cut(s) 150, 898, 2222
Eam1105I GACNNNNNGTC 1 cut(s) 1046
EarI CTCTTC 3 cut(s) 150, 898, 2222
Eco130I CCWWGG 2 cut(s) 561, 2755
Eco47I GGWCC 9 cut(s) 209, 269, 275, 558, 587, 671, 677, 683, 836
Eco57I CTGAAG 2 cut(s) 399, 1335
Eco81I CCTNAGG 1 cut(s) 1822
Eco88I CYCGRG 2 cut(s) 346, 1986
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 3 cut(s) 587, 671, 2639
EcoRI GAATTC 1 cut(s) 1548
EcoRII CCWGG 8 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641
EcoT14I CCWWGG 2 cut(s) 561, 2755
EcoT22I ATGCAT 2 cut(s) 851, 2484
ErhI CCWWGG 2 cut(s) 561, 2755
FalI AAGNNNNNCTT 2 cut(s) 1650, 1682
FaqI GGGAC 4 cut(s) 340, 571, 1884, 1903
FauNDI CATATG 1 cut(s) 937
FbaI TGATCA 2 cut(s) 34, 1852
FblI GTMKAC 1 cut(s) 1511
FokI GGATG 2 cut(s) 2853, 2944
FspAI RTGCGCAY 1 cut(s) 2718
FspBI CTAG 9 cut(s) 279, 522, 926, 1155, 1161, 1218, 1914, 2255, 2634
FspI TGCGCA 1 cut(s) 2718
GlaI GCGC 4 cut(s) 222, 870, 2156, 2718
GsaI CCCAGC 1 cut(s) 1475
GsuI CTGGAG 4 cut(s) 414, 1986, 2211, 2862
HaeIII GGCC 9 cut(s) 345, 643, 882, 977, 1395, 2136, 2626, 2640, 2662
HapII CCGG 4 cut(s) 108, 824, 2362, 2814
HgaI GACGC 7 cut(s) 81, 117, 1083, 1193, 1469, 1881, 2985
HhaI GCGC 4 cut(s) 223, 871, 2157, 2719
Hin1I GRCGYC 4 cut(s) 128, 329, 1873, 2977
Hin6I GCGC 4 cut(s) 221, 869, 2155, 2717
HinP1I GCGC 4 cut(s) 221, 869, 2155, 2717
HincII GTYRAC 1 cut(s) 2246
HindII GTYRAC 1 cut(s) 2246
HindIII AAGCTT 1 cut(s) 2858
HpaII CCGG 4 cut(s) 108, 824, 2362, 2814
Hpy166II GTNNAC 9 cut(s) 287, 839, 1073, 1400, 1512, 2177, 2246, 2308, 2390
Hpy188III TCNNGA 9 cut(s) 38, 253, 380, 393, 623, 1457, 1670, 2327, 2362
Hpy8I GTNNAC 9 cut(s) 287, 839, 1073, 1400, 1512, 2177, 2246, 2308, 2390
Hpy99I CGWCG 1 cut(s) 2013
HpyCH4III ACNGT 6 cut(s) 1842, 2057, 2089, 2426, 2609, 2669
HpyF3I CTNAG 7 cut(s) 57, 96, 606, 1689, 1822, 2221, 2856
Hsp92I GRCGYC 4 cut(s) 128, 329, 1873, 2977
HspAI GCGC 4 cut(s) 221, 869, 2155, 2717
Kpn2I TCCGGA 1 cut(s) 2361
KpnI GGTACC 2 cut(s) 26, 2802
Ksp22I TGATCA 2 cut(s) 34, 1852
KspI CCGCGG 1 cut(s) 3001
LmnI GCTCC 5 cut(s) 1438, 1459, 1817, 2110, 2515
LweI GCATC 6 cut(s) 898, 1189, 1214, 1598, 2422, 2922
MaeI CTAG 9 cut(s) 279, 522, 926, 1155, 1161, 1218, 1914, 2255, 2634
MaeIII GTNAC 4 cut(s) 193, 1150, 2091, 2650
MfeI CAATTG 4 cut(s) 735, 2434, 2562, 2702
MflI RGATCY 8 cut(s) 40, 110, 610, 1318, 2146, 2400, 2590, 2839
MhlI GDGCHC 2 cut(s) 924, 1063
MlsI TGGCCA 1 cut(s) 2136
MluNI TGGCCA 1 cut(s) 2136
MlyI GAGTC 3 cut(s) 130, 1035, 1082
MmeI TCCRAC 4 cut(s) 1089, 2557, 2568, 2618
Mox20I TGGCCA 1 cut(s) 2136
Mph1103I ATGCAT 2 cut(s) 851, 2484
MroI TCCGGA 1 cut(s) 2361
MroXI GAANNNNTTC 1 cut(s) 1711
MscI TGGCCA 1 cut(s) 2136
MseI TTAA 7 cut(s) 1728, 2100, 2112, 2282, 2343, 2621, 2942
MslI CAYNNNNRTG 4 cut(s) 468, 844, 957, 2477
Msp20I TGGCCA 1 cut(s) 2136
MspA1I CMGCKG 7 cut(s) 419, 455, 605, 1190, 1331, 2189, 3000
MspI CCGG 4 cut(s) 108, 824, 2362, 2814
MspR9I CCNGG 9 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643, 2815
MunI CAATTG 4 cut(s) 735, 2434, 2562, 2702
Mva1269I GAATGC 3 cut(s) 247, 432, 2729
MvaI CCWGG 8 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643
MvnI CGCG 2 cut(s) 871, 3000
NciI CCSGG 1 cut(s) 2815
NcoI CCATGG 1 cut(s) 561
NdeI CATATG 1 cut(s) 937
NmeAIII GCCGAG 1 cut(s) 1630
NsbI TGCGCA 1 cut(s) 2718
NsiI ATGCAT 2 cut(s) 851, 2484
NspI RCATGY 4 cut(s) 701, 853, 1019, 1235
PaeI GCATGC 2 cut(s) 1019, 1235
PagI TCATGA 1 cut(s) 2326
PaqCI CACCTGC 1 cut(s) 1112
PasI CCCWGGG 1 cut(s) 2642
PciI ACATGT 1 cut(s) 697
PcsI WCGNNNNNNNCGW 1 cut(s) 2422
PctI GAATGC 3 cut(s) 247, 432, 2729
PdmI GAANNNNTTC 1 cut(s) 1711
PfeI GAWTC 7 cut(s) 11, 61, 116, 122, 1448, 1736, 2314
PflFI GACNNNGTC 2 cut(s) 2609, 2669
PflMI CCANNNNNTGG 1 cut(s) 692
PfoI TCCNGGA 2 cut(s) 673, 1543
PleI GAGTC 3 cut(s) 130, 1035, 1082
PpsI GAGTC 3 cut(s) 130, 1035, 1082
Ppu21I YACGTR 1 cut(s) 2714
PpuMI RGGWCCY 2 cut(s) 587, 671
PscI ACATGT 1 cut(s) 697
PshBI ATTAAT 1 cut(s) 2343
Psp5II RGGWCCY 2 cut(s) 587, 671
Psp6I CCWGG 8 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641
PspFI CCCAGC 1 cut(s) 1471
PspGI CCWGG 8 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641
PspPPI RGGWCCY 2 cut(s) 587, 671
PstI CTGCAG 8 cut(s) 1024, 1120, 1246, 1330, 1369, 1744, 1768, 1807
PstNI CAGNNNCTG 1 cut(s) 1457
PsuI RGATCY 8 cut(s) 40, 110, 610, 1318, 2146, 2400, 2590, 2839
PsyI GACNNNGTC 2 cut(s) 2609, 2669
PvuII CAGCTG 4 cut(s) 419, 605, 1190, 2189
RsaI GTAC 6 cut(s) 24, 1746, 2307, 2568, 2712, 2800
RsaNI GTAC 6 cut(s) 23, 1745, 2306, 2567, 2711, 2799
RseI CAYNNNNRTG 4 cut(s) 468, 844, 957, 2477
SacII CCGCGG 1 cut(s) 3001
SaqAI TTAA 7 cut(s) 1728, 2100, 2112, 2282, 2343, 2621, 2942
SbfI CCTGCAGG 1 cut(s) 1768
SchI GAGTC 3 cut(s) 130, 1035, 1082
ScrFI CCNGG 9 cut(s) 273, 675, 681, 987, 1392, 1404, 1545, 2643, 2815
SdaI CCTGCAGG 1 cut(s) 1768
SduI GDGCHC 2 cut(s) 924, 1063
SfaNI GCATC 6 cut(s) 898, 1189, 1214, 1598, 2422, 2922
SfcI CTRYAG 8 cut(s) 1020, 1116, 1242, 1326, 1365, 1740, 1764, 1803
Sfr303I CCGCGG 1 cut(s) 3001
SgrBI CCGCGG 1 cut(s) 3001
SinI GGWCC 9 cut(s) 209, 269, 275, 558, 587, 671, 677, 683, 836
SmiMI CAYNNNNRTG 4 cut(s) 468, 844, 957, 2477
SmlI CTYRAG 2 cut(s) 380, 2074
SmoI CTYRAG 2 cut(s) 380, 2074
SpeI ACTAGT 1 cut(s) 1154
SphI GCATGC 2 cut(s) 1019, 1235
Sse8387I CCTGCAGG 1 cut(s) 1768
SsiI CCGC 9 cut(s) 188, 200, 302, 453, 1331, 1598, 2802, 2998, 3000
SspMI CTAG 9 cut(s) 279, 522, 926, 1155, 1161, 1218, 1914, 2255, 2634
StyD4I CCNGG 9 cut(s) 271, 673, 679, 985, 1390, 1402, 1543, 2641, 2813
StyI CCWWGG 2 cut(s) 561, 2755
TaaI ACNGT 6 cut(s) 1842, 2057, 2089, 2426, 2609, 2669
TaqI TCGA 7 cut(s) 252, 494, 1774, 2208, 2416, 2501, 2613
TaqII GACCGA 1 cut(s) 2487
TatI WGTACW 1 cut(s) 2566
TauI GCSGC 2 cut(s) 190, 3003
TfiI GAWTC 7 cut(s) 11, 61, 116, 122, 1448, 1736, 2314
Tru1I TTAA 7 cut(s) 1728, 2100, 2112, 2282, 2343, 2621, 2942
Tru9I TTAA 7 cut(s) 1728, 2100, 2112, 2282, 2343, 2621, 2942
TspGWI ACGGA 1 cut(s) 2751
Tth111I GACNNNGTC 2 cut(s) 2609, 2669
Van91I CCANNNNNTGG 1 cut(s) 692
VpaK11BI GGWCC 9 cut(s) 209, 269, 275, 558, 587, 671, 677, 683, 836
VspI ATTAAT 1 cut(s) 2343
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 1 cut(s) 1548
XceI RCATGY 4 cut(s) 701, 853, 1019, 1235
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XmiI GTMKAC 1 cut(s) 1511
XmnI GAANNNNTTC 1 cut(s) 1711
XspI CTAG 9 cut(s) 279, 522, 926, 1155, 1161, 1218, 1914, 2255, 2634
ZraI GACGTC 1 cut(s) 330
Zsp2I ATGCAT 2 cut(s) 851, 2484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.