MD11G1174100.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002717
Physical Location & Seq
Reverse (-)
20340736 .. 20354133
13398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1174100.v1.1.491

Sequence Viewer

Length: 3009 bp
ATGGATGTGGACTCATCGTCGATGGTGCCGGAGACCGATCACGATCCGGCAGTACCAAACCACAGTACGACGTCGTCACCGGCCGCAGTGGAGGAGGAGCAACAGCTAGGCGAGCAGGCGCAACCGGGAACTGGATCTCCGCCGCAGCAGCCTCAGACGGCGGCGGCGGCTCCGCAGGCACAGCAGACTCCGGTGGCCGGGCCCAGGCACGCTCCAACATACTCGGTGGTCAATGCAATTTTAGAGAAAAAAGAAGACGGGCCAGGGCCCAGGTGTGGCCACACGTTGACTGCGGTAGCGGCCGTGGGAGAGGAGGGCACGCCGGGTTACATTGGCCCCAGGTTGATTTTATTTGGGGGTGCCACAGCTCTTGAGGGCAATTCGGCTGCTTCAGGGACTCCATCATCAGCTGGAAGTGCTGGCATAAGACTTGCAGGTGCCACCGCAGACGTGCATTGTTATGATGTGCTAACAAATAAATGGTCTAGGATCACACCGTTTGGAGAGCCTCCCACCCCAAGGGCTGCACATGTAGCAACAGCTGTAGGAACTATGGTGGTTATTCAGGGTGGAATTGGTCCCGCTGGTTTGTCTGCTGAGGATCTTCATGTTCTTGACCTCACACAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCTGGGCCACGGTATGGACATGTGATGGCATTAGTGGGGCAAAGATATCTTATGGCAATTGGTGGGAATGATGGAAAACGGCCATTAGCTGATGTTTGGGCTCTTGACACAGCTGCCAAACCTTATGAATGGCGTAAGTTGGAACCGGAGGGGGAGGGTCCACCACCATGCATGTATGCAACTGCAAGTGCAAGGTCTGATGGTCTTCTCCTCCTCTGCGGAGGGAGGGATGCAAATAGTGTGCCATTGGCAAGTGCATATGGTCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCACCTTCTTCAAGATATCAACATGCAGCAGTCTTTGTTAATGCACGTCTACATGTTTCTGGAGGGGCCCTTGGTGGTGGCCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCAGGAGTTTGGTGTGATACAAAATCTGTGGTTACTAGTCCAAGGACAGGTAGATACAGTGCTGATGCAGCCGGTGGGGATGCTGCAGTTGAGCTGACAAGGCGTTGTAGGCATGCAGCTGCAGCGGTTGGTGACTTGATCTTTATATATGGTGGTCTACGTGGTGGAGTGCTGCTTGATGACCTACTTGTTGCGGAAGATTTGGCTGCTGCTGAAACAACAACTGCAGCTTCACATGCTGCAGCTGCAGCTGCTCAATCTGTACAAGGTCGTTTACCTGGAAGGTATGGATTCATTGATGACAGAGCAAGGCAGTTGCCTGAAGCAGCCCCTGATGGTGCTGTTGTGCTGGGAAATCCAGTAGCCCCCCCAGTAAATGGTGACATGTATACTGATATAAGTACTGAAAATGCTTTGCTCCAGGGATCCCGGAGAATTAGCAAAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGTGCCACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTTGAACTACCTGATAGAGATCGTGGTTCAGAGGCTACCCCCAGTGGGAAGCAGATATCTACCTTGATCAAGCCTGATTCTGCTGGGTCAAATAATATTCCTTCCGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGTGGAGCTTTAGGTGGCATGGTGAGGCAACTTTCAATTGATCAATTTGAAAATGAAGGCAGGCGGGTTAGTTATGGGACTCCAGAGAGTGCTACTGCAGCAAGGAAATTGTTAGATCGACAGATGTCCATTAATAGTGTGCCCAAAAAGGTAGTGGCACATCTTTTAAAGCCTCGTGGGTGGAAGCCTCCGGTTCGTAGGCAATTTTTCTTAGATTGTAATGAAATAGCAGATCTTTGTGACAGTGCTGAGCGAATATTCTCTAGTGAACCAAGCGTCTTACAGCTTAGGGCTCCTATCAAGATATTTGGTGATTTACATGGGCAGTTTGGTGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACGGCTGGGGATATTGCATATATCGACTATCTCTTCTTAGGAGATTATGTTGACCGGGGCCAACATAGCTTGGAAACCATTACTCTTCTGCTTGCTTTGAAGGTTGAGTATCCCCAGAATGTACATTTAATTCGTGGCAACCATGAAGCTGCAGATATCAATGCTCTATTTGGCTTTCGGATTGAATGCATTGAACGAATGGGTGAGAGAGATGGAATTTGGGCATGGCATCGAATAAACCGTTTGTTTAATTGGCTTCCGCTAGCAGCTCTGATTGAAAAGAAAATCATTTGTATGCATGGCGGTATTGGTCGGTCTATAAATCATGTGGAACAGATTGAGAATCTCCAGCGTCCTATTACAATGGAAGCTGGCTCCATTGTGCTTATGGATTTGTTATGGTCTGACCCAACAGAAAATGACAGTGTAGAAGGTTTAAGACCAAATGCTAGGGGTCCTGGGTTGGTTACATTTGGGCCTGATCGTGTCATGGAATTCTGCAATAACAATGATCTTCAGTTGATTGTACGTGCGCATGAATGTGTAATGGATGGATTTGAGCGTTTTGCCCAGGGGCATCTGATTACACTTTTCTCTGCTACCAATTATTGTGGTACGGCCAATAATGCAGGGGCAATCCTAGTCTTGGGTAGGGATCTTGTGGTGGTTCCAAAACTCATTCATCCCCTACCACCGGCAATTTCATCACCAGAAACTTCACCGGAACGCCATATTGAAGACACATGGATGCAGGAGTTGAATGCAAACAGACCTCCAACACCGACTAGAGGCCGTCCCCAAGTAGCTAATGATCGGGGTTCCCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

1003

Amino Acids

106.68

Weight (kDa)

5.72

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 83 - 297 1.2e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 84 - 261 7.2e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 146 - 424 2.8e-10 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 249 - 387 8e-12 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 265 - 388 4e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 333 - 379 1.6e-06 Kelch motif
Metallophos PF00149 699 - 906 6.6e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 425
AatII GACGTC 1 cut(s) 74
Acc16I TGCGCA 1 cut(s) 2706
Acc36I ACCTGC 1 cut(s) 425
AccB1I GGYRCC 3 cut(s) 25, 359, 437
AccB7I CCANNNNNTGG 2 cut(s) 683, 1487
AccI GTMKAC 3 cut(s) 1033, 1267, 1499
AclWI GGATC 7 cut(s) 38, 142, 497, 609, 1530, 1543, 2835
AcoI YGGCCR 8 cut(s) 81, 195, 277, 300, 749, 952, 1063, 2790
AcsI RAATTY 2 cut(s) 2388, 2666
AcuI CTGAAG 3 cut(s) 375, 1452, 2672
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 7 cut(s) 54, 67, 1374, 1513, 2295, 2700, 2788
AflIII ACRYGT 5 cut(s) 282, 529, 688, 1036, 1494
AhdI GACNNNNNGTC 1 cut(s) 16
AhlI ACTAGT 1 cut(s) 1145
AjiI CACGTC 2 cut(s) 451, 1031
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
Alw26I GTCTC 3 cut(s) 26, 988, 1790
AlwI GGATC 7 cut(s) 38, 142, 497, 609, 1530, 1543, 2835
AlwNI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
ApaI GGGCCC 3 cut(s) 204, 270, 1054
ApoI RAATTY 2 cut(s) 2388, 2666
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AseI ATTAAT 1 cut(s) 1932
AspLEI GCGC 3 cut(s) 121, 2145, 2707
AsuC2I CCSGG 5 cut(s) 126, 199, 324, 1540, 2228
AsuNHI GCTAGC 1 cut(s) 2434
AvaII GGWCC 4 cut(s) 578, 668, 827, 2627
BaeGI GKGCMC 5 cut(s) 204, 270, 320, 1054, 1944
BalI TGGCCA 1 cut(s) 279
BamHI GGATCC 1 cut(s) 1535
BanI GGYRCC 3 cut(s) 25, 359, 437
BanII GRGCYC 5 cut(s) 204, 270, 772, 1054, 2095
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BauI CACGAG 1 cut(s) 1974
BbsI GAAGAC 3 cut(s) 261, 866, 2916
BbvCI CCTCAGC 2 cut(s) 597, 1593
BceAI ACGGC 8 cut(s) 174, 287, 647, 764, 939, 2190, 2805, 2949
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciVI GTATCC 2 cut(s) 1096, 2292
BclI TGATCA 2 cut(s) 1713, 1840
BcnI CCSGG 5 cut(s) 126, 199, 324, 1540, 2228
BcoDI GTCTC 3 cut(s) 26, 988, 1790
BcuI ACTAGT 1 cut(s) 1145
BfaI CTAG 8 cut(s) 107, 486, 1146, 2064, 2435, 2622, 2813, 2958
BfuAI ACCTGC 1 cut(s) 425
BfuI GTATCC 2 cut(s) 1096, 2292
BglI GCCNNNNNGGC 2 cut(s) 640, 1613
BglII AGATCT 1 cut(s) 2032
BlpI GCTNAGC 1 cut(s) 2049
BmcAI AGTACT 1 cut(s) 1513
Bme18I GGWCC 4 cut(s) 578, 668, 827, 2627
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgBI CACGTC 2 cut(s) 451, 1031
BmrI ACTGGG 2 cut(s) 1475, 1683
BmsI GCATC 8 cut(s) 889, 1165, 1180, 1586, 1777, 2410, 2758, 2910
BmtI GCTAGC 1 cut(s) 2438
BmuI ACTGGG 2 cut(s) 1475, 1683
BoxI GACNNNNGTC 1 cut(s) 1924
BpiI GAAGAC 3 cut(s) 261, 866, 2916
BpmI CTGGAG 5 cut(s) 1065, 1514, 1776, 1866, 2504
Bpu10I CCTNAGC 3 cut(s) 597, 1593, 2087
Bpu1102I GCTNAGC 1 cut(s) 2049
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 5 cut(s) 126, 199, 324, 1540, 2228
BsaAI YACGTR 2 cut(s) 1271, 2702
BsaBI GATNNNNATC 2 cut(s) 42, 1665
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 26
BsaWI WCCGGW 4 cut(s) 190, 814, 1990, 2893
BsaXI ACNNNNNCTCC 4 cut(s) 121, 151, 2205, 2235
Bse118I RCCGGY 3 cut(s) 79, 1181, 2866
Bse1I ACTGG 5 cut(s) 136, 1469, 1481, 1689, 1804
Bse8I GATNNNNATC 2 cut(s) 42, 1665
BseGI GGATG 7 cut(s) 10, 904, 955, 1195, 2728, 2854, 2925
BseJI GATNNNNATC 2 cut(s) 42, 1665
BseMII CTCAG 4 cut(s) 167, 588, 1584, 2040
BseNI ACTGG 5 cut(s) 136, 1469, 1481, 1689, 1804
BseRI GAGGAG 5 cut(s) 107, 110, 326, 869, 872
BseSI GKGCMC 5 cut(s) 204, 270, 320, 1054, 1944
BseX3I CGGCCG 2 cut(s) 81, 300
BseYI CCCAGC 3 cut(s) 1459, 1730, 2177
BsgI GTGCAG 1 cut(s) 510
Bsh1285I CGRYCG 2 cut(s) 84, 303
BshNI GGYRCC 3 cut(s) 25, 359, 437
BsiEI CGRYCG 2 cut(s) 84, 303
BslFI GGGAC 4 cut(s) 409, 564, 1891, 2952
BsmAI GTCTC 3 cut(s) 26, 988, 1790
BsmFI GGGAC 4 cut(s) 409, 564, 1891, 2952
BsmI GAATGC 2 cut(s) 2363, 2938
Bso31I GGTCTC 1 cut(s) 26
Bsp120I GGGCCC 3 cut(s) 200, 266, 1050
Bsp1286I GDGCHC 7 cut(s) 204, 270, 320, 772, 1054, 1944, 2095
Bsp1407I TGTACA 2 cut(s) 1372, 2293
Bsp1720I GCTNAGC 1 cut(s) 2049
BspCNI CTCAG 4 cut(s) 166, 589, 1585, 2041
BspMAI CTGCAG 9 cut(s) 1111, 1198, 1234, 1339, 1354, 1360, 1795, 1900, 2326
BspMI ACCTGC 1 cut(s) 425
BspOI GCTAGC 1 cut(s) 2438
BspPI GGATC 7 cut(s) 38, 142, 497, 609, 1530, 1543, 2835
BspT107I GGYRCC 3 cut(s) 25, 359, 437
BspTNI GGTCTC 1 cut(s) 26
BsrFI RCCGGY 3 cut(s) 79, 1181, 2866
BsrGI TGTACA 2 cut(s) 1372, 2293
BsrI ACTGG 5 cut(s) 136, 1469, 1481, 1689, 1804
BssAI RCCGGY 3 cut(s) 79, 1181, 2866
BssNAI GTATAC 1 cut(s) 1500
BssNI GRCGYC 1 cut(s) 71
BssSI CACGAG 1 cut(s) 1974
BssT1I CCWWGG 4 cut(s) 518, 1054, 1151, 1623
Bst1107I GTATAC 1 cut(s) 1500
Bst2BI CACGAG 1 cut(s) 1974
Bst4CI ACNGT 7 cut(s) 65, 498, 681, 1169, 2045, 2414, 2597
Bst6I CTCTTC 2 cut(s) 2210, 2262
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 1604
BstAUI TGTACA 2 cut(s) 1372, 2293
BstBAI YACGTR 2 cut(s) 1271, 2702
BstDEI CTNAG 7 cut(s) 153, 597, 1593, 2011, 2049, 2087, 2209
BstDSI CCRYGG 2 cut(s) 303, 677
BstF5I GGATG 7 cut(s) 10, 904, 955, 1195, 2728, 2854, 2925
BstHHI GCGC 3 cut(s) 121, 2145, 2707
BstMAI GTCTC 3 cut(s) 26, 988, 1790
BstMCI CGRYCG 2 cut(s) 84, 303
BstNSI RCATGY 8 cut(s) 533, 692, 844, 1010, 1040, 1226, 1349, 1498
BstPAI GACNNNNGTC 1 cut(s) 1924
BstSLI GKGCMC 5 cut(s) 204, 270, 320, 1054, 1944
BstV2I GAAGAC 3 cut(s) 261, 866, 2916
BstX2I RGATCY 5 cut(s) 134, 601, 1535, 2032, 2827
BstYI RGATCY 5 cut(s) 134, 601, 1535, 2032, 2827
BstZ17I GTATAC 1 cut(s) 1500
BstZI CGGCCG 2 cut(s) 81, 300
BsuI GTATCC 2 cut(s) 1096, 2292
BtgI CCRYGG 2 cut(s) 303, 677
BtrI CACGTC 2 cut(s) 451, 1031
BtsCI GGATG 7 cut(s) 10, 904, 955, 1195, 2728, 2854, 2925
BtsI GCAGTG 2 cut(s) 93, 1101
BtsIMutI CAGTG 7 cut(s) 93, 1101, 1174, 1609, 1696, 2050, 2602
BveI ACCTGC 1 cut(s) 425
CaiI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
CfoI GCGC 3 cut(s) 121, 2145, 2707
Cfr10I RCCGGY 3 cut(s) 79, 1181, 2866
CseI GACGC 2 cut(s) 2065, 2513
Csp6I GTAC 7 cut(s) 53, 66, 1373, 1512, 2294, 2699, 2787
CspCI CAANNNNNGTGG 6 cut(s) 352, 387, 1119, 1154, 2764, 2799
CviQI GTAC 7 cut(s) 53, 66, 1373, 1512, 2294, 2699, 2787
DdeI CTNAG 7 cut(s) 153, 597, 1593, 2011, 2049, 2087, 2209
DraI TTTAAA 1 cut(s) 1968
DriI GACNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 8 cut(s) 81, 195, 277, 300, 749, 952, 1063, 2790
EagI CGGCCG 2 cut(s) 81, 300
Eam1104I CTCTTC 2 cut(s) 2210, 2262
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 2 cut(s) 2210, 2262
EciI GGCGGA 1 cut(s) 129
EclXI CGGCCG 2 cut(s) 81, 300
Eco130I CCWWGG 4 cut(s) 518, 1054, 1151, 1623
Eco24I GRGCYC 5 cut(s) 204, 270, 772, 1054, 2095
Eco31I GGTCTC 1 cut(s) 26
Eco32I GATATC 4 cut(s) 716, 1001, 1704, 2329
Eco47I GGWCC 4 cut(s) 578, 668, 827, 2627
Eco52I CGGCCG 2 cut(s) 81, 300
Eco57I CTGAAG 3 cut(s) 375, 1452, 2672
EcoO109I RGGNCCY 5 cut(s) 266, 662, 1050, 1051, 2627
EcoRI GAATTC 1 cut(s) 2666
EcoRV GATATC 4 cut(s) 716, 1001, 1704, 2329
EcoT14I CCWWGG 4 cut(s) 518, 1054, 1151, 1623
EcoT22I ATGCAT 3 cut(s) 842, 2363, 2472
EcoT38I GRGCYC 5 cut(s) 204, 270, 772, 1054, 2095
ErhI CCWWGG 4 cut(s) 518, 1054, 1151, 1623
FaqI GGGAC 4 cut(s) 409, 564, 1891, 2952
FauI CCCGC 2 cut(s) 589, 1857
FauNDI CATATG 1 cut(s) 928
FbaI TGATCA 2 cut(s) 1713, 1840
FblI GTMKAC 3 cut(s) 1033, 1267, 1499
FokI GGATG 7 cut(s) 17, 911, 962, 1202, 2735, 2841, 2932
FriOI GRGCYC 5 cut(s) 204, 270, 772, 1054, 2095
FspAI RTGCGCAY 1 cut(s) 2706
FspBI CTAG 8 cut(s) 107, 486, 1146, 2064, 2435, 2622, 2813, 2958
FspI TGCGCA 1 cut(s) 2706
GlaI GCGC 3 cut(s) 120, 2144, 2706
GsaI CCCAGC 3 cut(s) 1463, 1734, 2181
GsuI CTGGAG 5 cut(s) 1065, 1514, 1776, 1866, 2504
HgaI GACGC 2 cut(s) 2065, 2513
HhaI GCGC 3 cut(s) 121, 2145, 2707
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 3 cut(s) 119, 2143, 2705
HinP1I GCGC 3 cut(s) 119, 2143, 2705
HincII GTYRAC 2 cut(s) 288, 2224
HindII GTYRAC 2 cut(s) 288, 2224
HinfI GANTC 8 cut(s) 11, 187, 397, 1079, 1401, 1724, 1879, 2515
Hpy188I TCNGA 7 cut(s) 156, 868, 1678, 2352, 2445, 2578, 2754
Hpy188III TCNNGA 8 cut(s) 41, 371, 614, 773, 996, 1044, 1883, 2101
Hpy99I CGWCG 3 cut(s) 22, 73, 76
HpyAV CCTTC 7 cut(s) 999, 1386, 1758, 1850, 2178, 2266, 2597
HpyCH4III ACNGT 7 cut(s) 65, 498, 681, 1169, 2045, 2414, 2597
HpyCH4IV ACGT 6 cut(s) 71, 284, 450, 1030, 1270, 2701
HpyF3I CTNAG 7 cut(s) 153, 597, 1593, 2011, 2049, 2087, 2209
HpySE526I ACGT 6 cut(s) 71, 284, 450, 1030, 1270, 2701
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 3 cut(s) 119, 2143, 2705
Ksp22I TGATCA 2 cut(s) 1713, 1840
LmnI GCTCC 8 cut(s) 97, 175, 217, 979, 1533, 1805, 2098, 2552
LweI GCATC 8 cut(s) 889, 1165, 1180, 1586, 1777, 2410, 2758, 2910
MaeI CTAG 8 cut(s) 107, 486, 1146, 2064, 2435, 2622, 2813, 2958
MaeII ACGT 6 cut(s) 71, 284, 450, 1030, 1270, 2701
MaeIII GTNAC 7 cut(s) 75, 326, 1141, 1241, 1490, 2039, 2638
MfeI CAATTG 3 cut(s) 726, 969, 1836
MflI RGATCY 5 cut(s) 134, 601, 1535, 2032, 2827
MhlI GDGCHC 7 cut(s) 204, 270, 320, 772, 1054, 1944, 2095
MlsI TGGCCA 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 4 cut(s) 5, 181, 391, 1873
MmeI TCCRAC 4 cut(s) 239, 789, 1080, 2972
Mox20I TGGCCA 1 cut(s) 279
Mph1103I ATGCAT 3 cut(s) 842, 2363, 2472
MscI TGGCCA 1 cut(s) 279
MseI TTAA 7 cut(s) 1023, 1638, 1932, 1967, 2300, 2421, 2609
MslI CAYNNNNRTG 6 cut(s) 459, 835, 948, 2465, 2712, 2918
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 9 cut(s) 410, 542, 584, 782, 1229, 1235, 1355, 1361, 1754
MunI CAATTG 3 cut(s) 726, 969, 1836
Mva1269I GAATGC 2 cut(s) 2363, 2938
NciI CCSGG 5 cut(s) 126, 199, 324, 1540, 2228
NdeI CATATG 1 cut(s) 928
NheI GCTAGC 1 cut(s) 2434
NmuCI GTSAC 4 cut(s) 75, 1241, 1490, 2039
NsbI TGCGCA 1 cut(s) 2706
NsiI ATGCAT 3 cut(s) 842, 2363, 2472
NspI RCATGY 8 cut(s) 533, 692, 844, 1010, 1040, 1226, 1349, 1498
PaeI GCATGC 1 cut(s) 1226
PaqCI CACCTGC 1 cut(s) 425
PasI CCCWGGG 1 cut(s) 2743
PciI ACATGT 4 cut(s) 529, 688, 1036, 1494
PcsI WCGNNNNNNNCGW 1 cut(s) 2410
PctI GAATGC 2 cut(s) 2363, 2938
PfeI GAWTC 4 cut(s) 1079, 1401, 1724, 2515
PflFI GACNNNGTC 1 cut(s) 73
PflMI CCANNNNNTGG 2 cut(s) 683, 1487
PfoI TCCNGGA 1 cut(s) 1538
PleI GAGTC 4 cut(s) 5, 181, 391, 1873
PpsI GAGTC 4 cut(s) 5, 181, 391, 1873
Ppu21I YACGTR 2 cut(s) 1271, 2702
PpuMI RGGWCCY 1 cut(s) 2627
PscI ACATGT 4 cut(s) 529, 688, 1036, 1494
PshAI GACNNNNGTC 1 cut(s) 1924
PshBI ATTAAT 1 cut(s) 1932
Psp5II RGGWCCY 1 cut(s) 2627
PspFI CCCAGC 3 cut(s) 1459, 1730, 2177
PspOMI GGGCCC 3 cut(s) 200, 266, 1050
PspPPI RGGWCCY 1 cut(s) 2627
PstI CTGCAG 9 cut(s) 1111, 1198, 1234, 1339, 1354, 1360, 1795, 1900, 2326
PstNI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
PsuI RGATCY 5 cut(s) 134, 601, 1535, 2032, 2827
PsyI GACNNNGTC 1 cut(s) 73
PvuII CAGCTG 6 cut(s) 410, 542, 782, 1229, 1355, 1361
RsaI GTAC 7 cut(s) 54, 67, 1374, 1513, 2295, 2700, 2788
RsaNI GTAC 7 cut(s) 53, 66, 1373, 1512, 2294, 2699, 2787
RseI CAYNNNNRTG 6 cut(s) 459, 835, 948, 2465, 2712, 2918
SaqAI TTAA 7 cut(s) 1023, 1638, 1932, 1967, 2300, 2421, 2609
ScaI AGTACT 1 cut(s) 1513
SchI GAGTC 4 cut(s) 5, 181, 391, 1873
SduI GDGCHC 7 cut(s) 204, 270, 320, 772, 1054, 1944, 2095
SfaNI GCATC 8 cut(s) 889, 1165, 1180, 1586, 1777, 2410, 2758, 2910
SinI GGWCC 4 cut(s) 578, 668, 827, 2627
SmiMI CAYNNNNRTG 6 cut(s) 459, 835, 948, 2465, 2712, 2918
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 1145
SphI GCATGC 1 cut(s) 1226
SspI AATATT 2 cut(s) 1744, 2058
SspMI CTAG 8 cut(s) 107, 486, 1146, 2064, 2435, 2622, 2813, 2958
StyI CCWWGG 4 cut(s) 518, 1054, 1151, 1623
TaaI ACNGT 7 cut(s) 65, 498, 681, 1169, 2045, 2414, 2597
TaiI ACGT 6 cut(s) 74, 287, 453, 1033, 1273, 2704
TaqI TCGA 5 cut(s) 20, 637, 1918, 2196, 2404
TaqII GACCGA 2 cut(s) 50, 2475
TatI WGTACW 3 cut(s) 1372, 1511, 2293
TauI GCSGC 7 cut(s) 86, 145, 164, 167, 170, 302, 1068
TfiI GAWTC 4 cut(s) 1079, 1401, 1724, 2515
Tru1I TTAA 7 cut(s) 1023, 1638, 1932, 1967, 2300, 2421, 2609
Tru9I TTAA 7 cut(s) 1023, 1638, 1932, 1967, 2300, 2421, 2609
TscAI CASTG 7 cut(s) 93, 1101, 1174, 1609, 1696, 2050, 2602
TseFI GTSAC 4 cut(s) 75, 1241, 1490, 2039
Tsp45I GTSAC 4 cut(s) 75, 1241, 1490, 2039
TspRI CASTG 7 cut(s) 93, 1101, 1174, 1609, 1696, 2050, 2602
Tth111I GACNNNGTC 1 cut(s) 73
Van91I CCANNNNNTGG 2 cut(s) 683, 1487
VpaK11BI GGWCC 4 cut(s) 578, 668, 827, 2627
VspI ATTAAT 1 cut(s) 1932
XapI RAATTY 2 cut(s) 2388, 2666
XceI RCATGY 8 cut(s) 533, 692, 844, 1010, 1040, 1226, 1349, 1498
XcmI CCANNNNNNNNNTGG 2 cut(s) 1951, 2557
XmiI GTMKAC 3 cut(s) 1033, 1267, 1499
XspI CTAG 8 cut(s) 107, 486, 1146, 2064, 2435, 2622, 2813, 2958
ZraI GACGTC 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 1513
Zsp2I ATGCAT 3 cut(s) 842, 2363, 2472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.