RLG00000034543

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002717
Physical Location & Seq
Reverse (-)
44609508 .. 44620911
11404 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034543

Sequence Viewer

Length: 3015 bp
ATGGATGTGGACTCCTCGATGGCGCCGGAGGCCGATCACGATCCGGCTGTGCAGAACCAGAGCACGGCGGCAGCAGCAGTGGAGGGAGAGCAGCTAGGAGAACAGCCTCAGTCGCCGGCGTCCCCGGCGACGCAACAGCCTCAGTCGCCGCAGTCGCAGCAGCAGCAGCAGCAGCAGCAGCAGACTCCGGTGGCCGGGCCGCGGCATGCCCCGACCTACACGGTGGTCAATGCAATTTTAGAGAAGAAGGAGGACGGGCCAGGCCCGAGGTGTGGCCATACCTTGACTGCGGTCGCCGCTGTTGGCGAGGAGGGCAGTCCTGGGTACATTGGCCCCAGGCTGATATTGTTCGGAGGCGCCACCGCCCTTGAAGGCAATTCTGCGGCCTCAGGGACTCCATCTTCAGCTGGAAGTGCCGGAATAAGACTAGCTGGTGCCACAGCTGATGTGCACTGTTATGACATCCTAACAAATAAATGGTCTAGGATCACTCCCTTTGGAGAACCACCAACACCAAGGGCAGCACATGTAGCAACTGCTGTAGGAACTATGGTTGTTATTCAGGGAGGAATTGGTCCTGCTGGTTTGTCTGCTGAAGATTTACATGTTCTTGACCTCACTCAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCAGGGCCACGCTACGGACATGTGATGGCATTGGTGGGGCAAAGATATCTCATGGCAATTGGTGGGAATGATGGTAAACGGCCTTTAGCTGATGTTTGGGCGCTGGACACAGCTGCCAAGCCATATGAATGGCGTAAGTTGGAACCAGAGGGGGAGGGTCCACCACCATGCATGTATGCAACTGCAAGTGCAAGGTCGGATGGTCTTCTCCTCCTTTGTGGAGGGAGGGATGCAAACAGTGTGCCATTGGCAAGTGCATATGGCCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCCCCTTCTCCCAGATATCAACATGCAGCAGTTTTTGTTAATGCACGCTTGCATGTTTCTGGAGGGGCCCTTGGTGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCCGGGGTTTGGTGCGATACAAAATCTGTTGTCACGAGTCCAAGGACAGGTAGATACAGCGCTGATGCAGCTGGTGGAGATGCTGCAGTTGAATTGACACGGCGTTGCAGGCATGCAGCTGCCGCGGTTGGTGACCTGATATTTATATACGGTGGTCTACGTGGTGGGGTGTTGCTTGATGACCTACTGGTTGCGGAAGATCTGGCTGCTGCAGAAACAACAACTGCAGCCTCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGCCGTTTGCCTGGAAGATATGGATTCATTGATGAAAGAACGAGGCAGATAATGCCTGAAGCAGCACCTGATGGTGCTGTTGTGCTGGGAAGTCCAGTTGCCCCACCTGTAAATGGAGACATGTATACTGATATAAGCACTGAAAATGCCTTGCTCCAGGGATCCAGGAGACTGAGCAAAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCACTGCTACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTAGAGTTACCTGATAGAGATCGTGGCTCAGAGGCTACCCCGAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCGTCAGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGGGGAGCTTTGGGCGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCGGAGAGTGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCTAAAAAGGTAATAGCACATCTTTTAAAGCCACGTGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTTTGTGATAGTGCTGAACGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATATTTGGTGATTTACACGGGCAATTTGGCGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACTGCTGGGGATATTGCATATATCGACTATCTCTTCCTAGGAGACTATGTTGACCGAGGCCAACATAGCTTGGAAACCATTTCTCTTCTGCTTGCTTTGAAGGTTGAATATTCCCAAAATGTACACTTAATTAGGGGTAATCATGAAGCTGCAGATATTAATGCTCTTTTTGGCTTTCGGATCGAATGCATTGAGCGAATGGGTGAGAGAGACGGAATATGGGCATGGCATCGAATAAACCGTTTGTTCAATTGGCTTCCTCTAGCTGCTCTAATTGAGAAGAAAATCATTTGTATGCATGGTGGTATTGGTCGGTCAATAAATCATGTTGAACAGATTGAGAATCTTCAGCGTCCTATTACAATGGAAGCAGGCTCGATCGTTCTTATGGATTTATTATGGTCTGACCCAACAGAAAATGATAGTGTGGAAGGATTGCGACCAAATGCAAGGGGTCCTGGATTGGTTACTTTTGGGCCTGATCGTGTCATGGAATTCTGCAACAACAATGATCTTCAGTTGATTGTACGTGCTCATGAATGTGTAATGGATGGCTTTGAACGATTTGCCCAGGGACATTTGATTACACTTTTCTCTGCTACCAATTACTGTGGTACAGCAAATAATGCAGGGGCAATCCTTGTTTTAGGTAGAGATCTTGTGGTGGTCCCGAAACTCATTCATCCCTTACCACCAGCAATTTCTTCACCAGAAACATCGCCCGAACGACATATTGAAGATACGTGGATGCAGGAACTGAATGCTAACAGACCTCCAACACCAACTAGAGGCCGTCCACAAGGAGCAAATGACCGGGGATCCCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

1005

Amino Acids

107.15

Weight (kDa)

5.74

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 81 - 296 1.2e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 82 - 260 4.5e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 145 - 421 1.7e-10 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 250 - 386 4.1e-12 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 265 - 431 8.5e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 331 - 378 6e-07 Kelch motif
Metallophos PF00149 701 - 908 8.1e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 22, 356, 434
AccBSI CCGCTC 1 cut(s) 1698
AccI GTMKAC 2 cut(s) 1264, 1505
AccII CGCG 2 cut(s) 202, 1232
AclWI GGATC 7 cut(s) 35, 494, 1536, 1549, 2366, 2991, 3004
AcoI YGGCCR 3 cut(s) 192, 274, 949
AcsI RAATTY 1 cut(s) 2672
AcuI CTGAAG 5 cut(s) 387, 615, 1458, 2510, 2678
AcvI CACGTG 1 cut(s) 1982
AcyI GRCGYC 3 cut(s) 23, 119, 357
AfaI GTAC 4 cut(s) 326, 2301, 2706, 2794
AfeI AGCGCT 1 cut(s) 1168
AflIII ACRYGT 4 cut(s) 526, 604, 685, 1500
Alw21I GWGCWC 3 cut(s) 65, 453, 2713
Alw26I GTCTC 6 cut(s) 985, 1491, 1543, 1796, 2214, 2382
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 7 cut(s) 35, 494, 1536, 1549, 2366, 2991, 3004
AlwNI CAGNNNCTG 4 cut(s) 1448, 1598, 1805, 2935
Ama87I CYCGRG 2 cut(s) 265, 1693
Aor51HI AGCGCT 1 cut(s) 1168
ApaI GGGCCC 1 cut(s) 1051
ApaLI GTGCAC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 2672
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 2 cut(s) 1938, 2337
AspA2I CCTAGG 1 cut(s) 2215
AspLEI GCGC 6 cut(s) 25, 359, 769, 1065, 1169, 2151
AsuC2I CCSGG 5 cut(s) 125, 196, 1111, 1889, 2993
AsuHPI GGTGA 6 cut(s) 1250, 1840, 2129, 2163, 2393, 2877
AvaI CYCGRG 2 cut(s) 265, 1693
AvaII GGWCC 6 cut(s) 575, 665, 824, 1884, 2633, 2845
AvrII CCTAGG 1 cut(s) 2215
AxyI CCTNAGG 1 cut(s) 388
BaeGI GKGCMC 2 cut(s) 453, 1051
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 2 cut(s) 1541, 2996
BanI GGYRCC 3 cut(s) 22, 356, 434
BanII GRGCYC 2 cut(s) 1051, 2101
BarI GAAGNNNNNNTAC 2 cut(s) 1644, 1676
BauI CACGAG 1 cut(s) 1141
BbrPI CACGTG 1 cut(s) 1982
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 3 cut(s) 65, 453, 2713
BbvCI CCTCAGC 1 cut(s) 1599
BceAI ACGGC 7 cut(s) 81, 644, 761, 936, 1223, 1368, 2955
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciVI GTATCC 1 cut(s) 1093
BclI TGATCA 1 cut(s) 1719
BcnI CCSGG 5 cut(s) 125, 196, 1111, 1889, 2993
BcoDI GTCTC 6 cut(s) 985, 1491, 1543, 1796, 2214, 2382
BfaI CTAG 7 cut(s) 95, 428, 483, 2070, 2216, 2441, 2964
BfoI RGCGCY 4 cut(s) 26, 360, 770, 1170
BfuI GTATCC 1 cut(s) 1093
BglI GCCNNNNNGGC 1 cut(s) 637
BglII AGATCT 3 cut(s) 1306, 2038, 2833
BlnI CCTAGG 1 cut(s) 2215
Bme18I GGWCC 6 cut(s) 575, 665, 824, 1884, 2633, 2845
BmeT110I CYCGRG 2 cut(s) 265, 1693
BmrI ACTGGG 1 cut(s) 1815
BmsI GCATC 7 cut(s) 886, 1162, 1177, 1592, 1783, 2416, 2916
BmuI ACTGGG 1 cut(s) 1815
BoxI GACNNNNGTC 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 3 cut(s) 1062, 1520, 1782
Bpu10I CCTNAGC 1 cut(s) 1599
BpuMI CCSGG 5 cut(s) 125, 196, 1111, 1889, 2993
BsaAI YACGTR 4 cut(s) 1268, 1982, 2708, 2922
BsaBI GATNNNNATC 2 cut(s) 39, 1671
BsaHI GRCGYC 3 cut(s) 23, 119, 357
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 2211, 2241
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 3 cut(s) 1299, 1475, 1810
Bse21I CCTNAGG 1 cut(s) 388
Bse3DI GCAATG 1 cut(s) 2032
Bse8I GATNNNNATC 2 cut(s) 39, 1671
BseGI GGATG 9 cut(s) 10, 462, 871, 901, 952, 1991, 2734, 2860, 2931
BseJI GATNNNNATC 2 cut(s) 39, 1671
BseMI GCAATG 1 cut(s) 2032
BseMII CTCAG 8 cut(s) 122, 155, 402, 635, 1376, 1544, 1590, 1695
BseNI ACTGG 3 cut(s) 1299, 1475, 1810
BseRI GAGGAG 3 cut(s) 4, 323, 866
BseSI GKGCMC 2 cut(s) 453, 1051
BseYI CCCAGC 3 cut(s) 1465, 1736, 2183
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 2 cut(s) 202, 1232
Bsh1285I CGRYCG 2 cut(s) 294, 2559
BshNI GGYRCC 3 cut(s) 22, 356, 434
BsiEI CGRYCG 2 cut(s) 294, 2559
BsiHKAI GWGCWC 3 cut(s) 65, 453, 2713
BsiHKCI CYCGRG 2 cut(s) 265, 1693
BslFI GGGAC 5 cut(s) 106, 406, 1897, 2766, 2831
BsmAI GTCTC 6 cut(s) 985, 1491, 1543, 1796, 2214, 2382
BsmBI CGTCTC 1 cut(s) 2382
BsmFI GGGAC 5 cut(s) 106, 406, 1897, 2766, 2831
BsmI GAATGC 2 cut(s) 2369, 2944
BsoBI CYCGRG 2 cut(s) 265, 1693
Bsp120I GGGCCC 1 cut(s) 1047
Bsp1286I GDGCHC 5 cut(s) 65, 453, 1051, 2101, 2713
Bsp1407I TGTACA 1 cut(s) 2299
BspCNI CTCAG 8 cut(s) 121, 154, 401, 634, 1375, 1545, 1591, 1694
BspFNI CGCG 2 cut(s) 202, 1232
BspHI TCATGA 2 cut(s) 2320, 2713
BspMAI CTGCAG 9 cut(s) 1108, 1195, 1321, 1336, 1351, 1357, 1801, 1906, 2332
BspPI GGATC 7 cut(s) 35, 494, 1536, 1549, 2366, 2991, 3004
BspT107I GGYRCC 3 cut(s) 22, 356, 434
BsrBI CCGCTC 1 cut(s) 1698
BsrDI GCAATG 1 cut(s) 2032
BsrFI RCCGGY 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 2299
BsrI ACTGG 3 cut(s) 1299, 1475, 1810
BssAI RCCGGY 1 cut(s) 115
BssNAI GTATAC 1 cut(s) 1506
BssNI GRCGYC 3 cut(s) 23, 119, 357
BssSI CACGAG 1 cut(s) 1141
BssT1I CCWWGG 5 cut(s) 515, 1051, 1148, 1629, 2215
Bst1107I GTATAC 1 cut(s) 1506
Bst2BI CACGAG 1 cut(s) 1141
Bst4CI ACNGT 6 cut(s) 223, 455, 905, 1259, 2420, 2789
Bst6I CTCTTC 2 cut(s) 2216, 2268
BstACI GRCGYC 3 cut(s) 23, 119, 357
BstAPI GCANNNNNTGC 3 cut(s) 1432, 1610, 2804
BstAUI TGTACA 1 cut(s) 2299
BstBAI YACGTR 4 cut(s) 1268, 1982, 2708, 2922
BstDEI CTNAG 9 cut(s) 108, 141, 388, 621, 1362, 1553, 1599, 1681, 2093
BstDSI CCRYGG 2 cut(s) 200, 1230
BstEII GGTNACC 1 cut(s) 1238
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 9 cut(s) 10, 462, 871, 901, 952, 1991, 2734, 2860, 2931
BstFNI CGCG 2 cut(s) 202, 1232
BstH2I RGCGCY 4 cut(s) 26, 360, 770, 1170
BstHHI GCGC 6 cut(s) 25, 359, 769, 1065, 1169, 2151
BstMAI GTCTC 6 cut(s) 985, 1491, 1543, 1796, 2214, 2382
BstMCI CGRYCG 2 cut(s) 294, 2559
BstPAI GACNNNNGTC 1 cut(s) 290
BstPI GGTNACC 1 cut(s) 1238
BstSLI GKGCMC 2 cut(s) 453, 1051
BstUI CGCG 2 cut(s) 202, 1232
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 5 cut(s) 1306, 1541, 2038, 2833, 2996
BstXI CCANNNNNNTGG 2 cut(s) 795, 1986
BstYI RGATCY 5 cut(s) 1306, 1541, 2038, 2833, 2996
BstZ17I GTATAC 1 cut(s) 1506
Bsu36I CCTNAGG 1 cut(s) 388
BsuI GTATCC 1 cut(s) 1093
BtgI CCRYGG 2 cut(s) 200, 1230
BtgZI GCGATG 1 cut(s) 2880
BtsCI GGATG 9 cut(s) 10, 462, 871, 901, 952, 1991, 2734, 2860, 2931
BtsI GCAGTG 3 cut(s) 84, 1098, 1608
BtsIMutI CAGTG 6 cut(s) 84, 451, 910, 1098, 1518, 1608
CaiI CAGNNNCTG 4 cut(s) 1448, 1598, 1805, 2935
CciI TCATGA 2 cut(s) 2320, 2713
CfoI GCGC 6 cut(s) 25, 359, 769, 1065, 1169, 2151
Cfr10I RCCGGY 1 cut(s) 115
Cfr42I CCGCGG 2 cut(s) 203, 1233
CseI GACGC 3 cut(s) 108, 139, 2519
Csp6I GTAC 4 cut(s) 325, 2300, 2705, 2793
CspCI CAANNNNNGTGG 4 cut(s) 349, 384, 2770, 2805
CviQI GTAC 4 cut(s) 325, 2300, 2705, 2793
DdeI CTNAG 9 cut(s) 108, 141, 388, 621, 1362, 1553, 1599, 1681, 2093
DinI GGCGCC 2 cut(s) 24, 358
DraI TTTAAA 1 cut(s) 1974
EaeI YGGCCR 3 cut(s) 192, 274, 949
Eam1104I CTCTTC 2 cut(s) 2216, 2268
EarI CTCTTC 2 cut(s) 2216, 2268
Eco130I CCWWGG 5 cut(s) 515, 1051, 1148, 1629, 2215
Eco24I GRGCYC 2 cut(s) 1051, 2101
Eco32I GATATC 2 cut(s) 713, 998
Eco47I GGWCC 6 cut(s) 575, 665, 824, 1884, 2633, 2845
Eco47III AGCGCT 1 cut(s) 1168
Eco57I CTGAAG 5 cut(s) 387, 615, 1458, 2510, 2678
Eco72I CACGTG 1 cut(s) 1982
Eco81I CCTNAGG 1 cut(s) 388
Eco88I CYCGRG 2 cut(s) 265, 1693
Eco91I GGTNACC 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoO109I RGGNCCY 5 cut(s) 659, 1047, 1048, 1884, 2633
EcoO65I GGTNACC 1 cut(s) 1238
EcoRI GAATTC 1 cut(s) 2672
EcoRV GATATC 2 cut(s) 713, 998
EcoT14I CCWWGG 5 cut(s) 515, 1051, 1148, 1629, 2215
EcoT22I ATGCAT 3 cut(s) 839, 2369, 2478
EcoT38I GRGCYC 2 cut(s) 1051, 2101
EgeI GGCGCC 2 cut(s) 24, 358
EheI GGCGCC 2 cut(s) 24, 358
ErhI CCWWGG 5 cut(s) 515, 1051, 1148, 1629, 2215
Esp3I CGTCTC 1 cut(s) 2382
FaqI GGGAC 5 cut(s) 106, 406, 1897, 2766, 2831
FauI CCCGC 2 cut(s) 1691, 1863
FauNDI CATATG 2 cut(s) 790, 925
FbaI TGATCA 1 cut(s) 1719
FblI GTMKAC 2 cut(s) 1264, 1505
FokI GGATG 9 cut(s) 17, 449, 878, 908, 959, 1998, 2741, 2847, 2938
FriOI GRGCYC 2 cut(s) 1051, 2101
FspBI CTAG 7 cut(s) 95, 428, 483, 2070, 2216, 2441, 2964
GlaI GCGC 6 cut(s) 24, 358, 768, 1064, 1168, 2150
GsaI CCCAGC 3 cut(s) 1469, 1740, 2187
GsuI CTGGAG 3 cut(s) 1062, 1520, 1782
HaeII RGCGCY 4 cut(s) 26, 360, 770, 1170
HgaI GACGC 3 cut(s) 108, 139, 2519
HhaI GCGC 6 cut(s) 25, 359, 769, 1065, 1169, 2151
Hin1I GRCGYC 3 cut(s) 23, 119, 357
Hin6I GCGC 6 cut(s) 23, 357, 767, 1063, 1167, 2149
HinP1I GCGC 6 cut(s) 23, 357, 767, 1063, 1167, 2149
HincII GTYRAC 1 cut(s) 2230
HindII GTYRAC 1 cut(s) 2230
HinfI GANTC 7 cut(s) 11, 184, 394, 1076, 1144, 1404, 2521
HphI GGTGA 6 cut(s) 1250, 1840, 2129, 2163, 2393, 2877
Hpy188I TCNGA 5 cut(s) 353, 865, 1684, 2358, 2584
Hpy188III TCNNGA 8 cut(s) 38, 611, 1041, 1141, 2107, 2321, 2714, 2848
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 7 cut(s) 241, 365, 996, 1856, 2184, 2272, 2603
HpyCH4III ACNGT 6 cut(s) 223, 455, 905, 1259, 2420, 2789
HpyCH4IV ACGT 4 cut(s) 1267, 1981, 2707, 2921
HpyF3I CTNAG 9 cut(s) 108, 141, 388, 621, 1362, 1553, 1599, 1681, 2093
HpySE526I ACGT 4 cut(s) 1267, 1981, 2707, 2921
Hsp92I GRCGYC 3 cut(s) 23, 119, 357
HspAI GCGC 6 cut(s) 23, 357, 767, 1063, 1167, 2149
KasI GGCGCC 2 cut(s) 22, 356
KflI GGGWCCC 1 cut(s) 1884
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 1719
KspI CCGCGG 2 cut(s) 203, 1233
LmnI GCTCC 5 cut(s) 976, 1539, 1811, 2104, 2981
LweI GCATC 7 cut(s) 886, 1162, 1177, 1592, 1783, 2416, 2916
MaeI CTAG 7 cut(s) 95, 428, 483, 2070, 2216, 2441, 2964
MaeII ACGT 4 cut(s) 1267, 1981, 2707, 2921
MaeIII GTNAC 4 cut(s) 1138, 1238, 1659, 2644
MbiI CCGCTC 1 cut(s) 1698
MfeI CAATTG 3 cut(s) 723, 966, 2428
MflI RGATCY 5 cut(s) 1306, 1541, 2038, 2833, 2996
MhlI GDGCHC 5 cut(s) 65, 453, 1051, 2101, 2713
MlsI TGGCCA 1 cut(s) 276
MluNI TGGCCA 1 cut(s) 276
Mly113I GGCGCC 2 cut(s) 23, 357
MlyI GAGTC 4 cut(s) 5, 178, 388, 1153
MmeI TCCRAC 4 cut(s) 786, 843, 1077, 2978
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 3 cut(s) 839, 2369, 2478
MreI CGCCGGCG 1 cut(s) 115
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 276
MseI TTAA 6 cut(s) 1020, 1644, 1938, 1973, 2306, 2337
MslI CAYNNNNRTG 7 cut(s) 456, 793, 832, 945, 1984, 2471, 2718
Msp20I TGGCCA 1 cut(s) 276
MunI CAATTG 3 cut(s) 723, 966, 2428
Mva1269I GAATGC 2 cut(s) 2369, 2944
MvnI CGCG 2 cut(s) 202, 1232
NaeI GCCGGC 1 cut(s) 117
NarI GGCGCC 2 cut(s) 23, 357
NciI CCSGG 5 cut(s) 125, 196, 1111, 1889, 2993
NdeI CATATG 2 cut(s) 790, 925
NgoMIV GCCGGC 1 cut(s) 115
NmuCI GTSAC 2 cut(s) 1138, 1238
NsiI ATGCAT 3 cut(s) 839, 2369, 2478
PaeI GCATGC 3 cut(s) 209, 1223, 1346
PagI TCATGA 2 cut(s) 2320, 2713
PasI CCCWGGG 1 cut(s) 2749
PciI ACATGT 4 cut(s) 526, 604, 685, 1500
PcsI WCGNNNNNNNCGW 1 cut(s) 2416
PctI GAATGC 2 cut(s) 2369, 2944
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 3 cut(s) 1076, 1404, 2521
PfoI TCCNGGA 2 cut(s) 1544, 2635
Ple19I CGATCG 1 cut(s) 2559
PleI GAGTC 4 cut(s) 5, 178, 388, 1152
PluTI GGCGCC 2 cut(s) 26, 360
PmaCI CACGTG 1 cut(s) 1982
PmlI CACGTG 1 cut(s) 1982
PpsI GAGTC 4 cut(s) 5, 178, 388, 1152
Ppu21I YACGTR 4 cut(s) 1268, 1982, 2708, 2922
PpuMI RGGWCCY 2 cut(s) 1884, 2633
PscI ACATGT 4 cut(s) 526, 604, 685, 1500
PshAI GACNNNNGTC 1 cut(s) 290
PshBI ATTAAT 2 cut(s) 1938, 2337
Psp5II RGGWCCY 2 cut(s) 1884, 2633
PspCI CACGTG 1 cut(s) 1982
PspEI GGTNACC 1 cut(s) 1238
PspFI CCCAGC 3 cut(s) 1465, 1736, 2183
PspOMI GGGCCC 1 cut(s) 1047
PspPPI RGGWCCY 2 cut(s) 1884, 2633
PstI CTGCAG 9 cut(s) 1108, 1195, 1321, 1336, 1351, 1357, 1801, 1906, 2332
PstNI CAGNNNCTG 4 cut(s) 1448, 1598, 1805, 2935
PsuI RGATCY 5 cut(s) 1306, 1541, 2038, 2833, 2996
PvuI CGATCG 1 cut(s) 2559
PvuII CAGCTG 8 cut(s) 407, 443, 779, 1178, 1226, 1352, 1358, 1760
RsaI GTAC 4 cut(s) 326, 2301, 2706, 2794
RsaNI GTAC 4 cut(s) 325, 2300, 2705, 2793
RseI CAYNNNNRTG 7 cut(s) 456, 793, 832, 945, 1984, 2471, 2718
SacII CCGCGG 2 cut(s) 203, 1233
SaqAI TTAA 6 cut(s) 1020, 1644, 1938, 1973, 2306, 2337
SchI GAGTC 4 cut(s) 5, 178, 388, 1153
SduI GDGCHC 5 cut(s) 65, 453, 1051, 2101, 2713
SfaNI GCATC 7 cut(s) 886, 1162, 1177, 1592, 1783, 2416, 2916
SfoI GGCGCC 2 cut(s) 24, 358
Sfr303I CCGCGG 2 cut(s) 203, 1233
SgrAI CRCCGGYG 1 cut(s) 115
SgrBI CCGCGG 2 cut(s) 203, 1233
SinI GGWCC 6 cut(s) 575, 665, 824, 1884, 2633, 2845
SmiMI CAYNNNNRTG 7 cut(s) 456, 793, 832, 945, 1984, 2471, 2718
SphI GCATGC 3 cut(s) 209, 1223, 1346
SspDI GGCGCC 2 cut(s) 22, 356
SspI AATATT 2 cut(s) 1750, 2288
SspMI CTAG 7 cut(s) 95, 428, 483, 2070, 2216, 2441, 2964
StyI CCWWGG 5 cut(s) 515, 1051, 1148, 1629, 2215
TaaI ACNGT 6 cut(s) 223, 455, 905, 1259, 2420, 2789
TaiI ACGT 4 cut(s) 1270, 1984, 2710, 2924
TaqI TCGA 6 cut(s) 17, 634, 2202, 2361, 2410, 2555
TaqII GACCGA 2 cut(s) 2247, 2481
TatI WGTACW 1 cut(s) 2299
TauI GCSGC 8 cut(s) 71, 151, 202, 205, 299, 386, 1232, 1824
TfiI GAWTC 3 cut(s) 1076, 1404, 2521
Tru1I TTAA 6 cut(s) 1020, 1644, 1938, 1973, 2306, 2337
Tru9I TTAA 6 cut(s) 1020, 1644, 1938, 1973, 2306, 2337
TscAI CASTG 6 cut(s) 84, 458, 910, 1098, 1525, 1615
TseFI GTSAC 2 cut(s) 1138, 1238
Tsp45I GTSAC 2 cut(s) 1138, 1238
TspDTI ATGAA 9 cut(s) 807, 1068, 1396, 1428, 1875, 2043, 2337, 2730, 2849
TspGWI ACGGA 4 cut(s) 696, 1743, 2074, 2406
TspRI CASTG 6 cut(s) 84, 458, 910, 1098, 1525, 1615
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 6 cut(s) 575, 665, 824, 1884, 2633, 2845
VspI ATTAAT 2 cut(s) 1938, 2337
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 1 cut(s) 2672
XmaJI CCTAGG 1 cut(s) 2215
XmiI GTMKAC 2 cut(s) 1264, 1505
XspI CTAG 7 cut(s) 95, 428, 483, 2070, 2216, 2441, 2964
Zsp2I ATGCAT 3 cut(s) 839, 2369, 2478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.