FvH4_3g26300

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002717
Physical Location & Seq
Forward (+)
19245184 .. 19258720
13537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g26300.t1

Sequence Viewer

Length: 2997 bp
ATGGACGTGGACTCCTCAATGGTGCCGGAGGCCGATCACGATCCGGCTGTACAGAACCAGAGCACGGCGGCGGTGGATGTGGGAGAACAGCTAGGAGAACAACAGCCTCAGGCGCCGGCTTCCCCGGCGGCGCAACAGCCTCAGTCTCCGGCGTCGCCGCAGCAGACTCCGGTGGCCGGACCGAGGCATGCCCCCACGTACTCGGTGGTCAATGCGATTTTAGAGAAGAAGGAGGATGGGCCGGGGCCGAGGTGTGGCCACACGTTGACTGCGGTCGCCGCTGTTGGCGAGGAGGGCAGTCCTGGGTACATTGGACCCCGGCTGATTCTGTTCGGCGGTGCCACCGCCCTTGAGGGCAATTCTGCGGCTTCTGGGACTCCATCGTCAGCTGGAAGTGCCGGAATAAGACTAGCCGGTGCCACAGCTGATGTGCACTGTTATGACATCCTAACAAACAAATGGTCTAGGATCACTCCCTTTGGAGAACCACCAACACCAAGAGCAGCACATGTAGCAACTGCTGTAGGAACTATGGTGGTTATTCAGGGAGGAATTGGTCCTGCTGGTTTGTCTGCTGAGGATCTTCATGTTCTTGACCTCACTCAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCAGGGCCGCGCTACGGCCATGTGATGGCATTGGTGGGGCAAAGATATCTCATGGCAATCGGTGGGAATGATGGAAAACGGCCTTTAGCTGATGTTTGGGCACTGGACACAGCTGCCAAGCCATATGAATGGCGTAAGTTAGAACCGGAGGGGGAGGGTCCACCACCATGCATGTATGCAACTGCAAGTGCAAGGTCAGATGGTCTTCTCCTCCTTTGTGGAGGGAGGGATGCAAACAGTGTGCCACTGGCAAGTGCATATGGCCTTGCGAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGAGTCTCCCCTTCTCCCCGATATCAACACGCAGCAGTCTTTGTTAATGCACGCTTGCATGTTTCTGGAGGGGCCCTAGGTGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTATTGGATACTGCAGCCGGGGTTTGGTGCGATACAAAATCTGTTGTTACTAGTCCAAGGACAGGTAGATACAGCGCTGATGCAGCTGGTGGAGACGCTGCAGTTGAGTTGACACGGCGTTGCAGGCATGCAGCTGCCGCAGTTGGTGACTTGATATTTATATACGGTGGCCTACGTGGTGGGGTGTTACTCGATGACCTACTGGTTGCAGAAGATCTAGCTGCTGCCGAAACAACATCTGCAGCATCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGTCGTTTGCCTGGAAGATATGGATTCATTGATGACAGAACGAGGCAGATAATGCCTGAAGCAGCTCCTGATGGTGCTGTCGTCTTGGGAAATCCAGTTGCCCCCCCTGTAAATGGAGACATGTATACTGATATAAGCACTGAAAATGCCTTGCTCCAGGGATCCAGGAGATTGAGCAAAGGTGTGGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGTGCCACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGGGAAGTTGAGTTGCCTGATAGAGATCGGGGCTCAGAGGCTACCCCTAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCATCAGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGGGGAGCTTTGGGTGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCGGAGAGCGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCCAAAAAGGTAATAGCACATCTTTTAAAGCCTCGTGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTTTGTGATAGTGCTGAGCGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATATTTGGTGATTTACATGGGCAATTTGGCGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACTGCCGGGGATATTGCATATATTGACTATCTCTTCCTAGGAGACTATGTTGACCGAGGCCAACATAGCTTGGAAACAATATCTCTTCTGCTTGCTTTGAAGGTTGAATATGCCCATAATGTACACTTAATTAGGGGTAATCATGAAGCTGCCGATATTAATGCCCTTTTTGGCTTCCGGATCGAATGCATTGAGCGGATGGGTGAGAGAGATGGAATATGGGCATGGCATCGAATAAACCGTTTGTTCAATTGGCTTCCTCTTGCCGCCCTGATTGAGAAGAAAATCATTTGTATGCATGGTGGTATTGGTCGGTCAATAAATCATGTTGAACAGATTGAGAATCTCCAGCGTCCTATTACGATGGAAGCAGGCTCGATCGTTCTTATGGATTTATTATGGTCTGACCCGACAGAAAATGATAGTGTGGAAGGATTGCGACCAAACGCTAGGGGTCCTGGATTGGTTACTTTTGGGCCTGATCGTGTCATGGAATTCTGCAACAACAATGATCTTCAGTTGATTGTACGCGCTCATGAATGTGTAATGGATGGCTTTGAACGATTTGCCCAGGGACATTTGATTACACTTTTCTCTGCTACCAACTACTGTGGTACAGCAAATAATGCAGGGGCAATCCTTGTATTAGGTAGAGATCTTGTGGTGGTTCCGAAACTCATTCATCCCTTACCACCAGCAATTTCTTCACCAGAAACATCACCCGAACGACATATTGAAGATACGTGGATGCAGGAACTGAATGCTAATAGACCACCAACACCAACTAGAGGCCGTCCACAAGTAGCAAATGACCGGGGCTCTCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

999

Amino Acids

106.35

Weight (kDa)

5.76

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 76 - 290 1.3e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 77 - 254 5.2e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 139 - 416 1e-10 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 244 - 380 3.9e-12 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 259 - 381 8.6e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 325 - 372 5.9e-07 Kelch motif
Metallophos PF00149 695 - 902 9.9e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 382
AccB1I GGYRCC 4 cut(s) 22, 112, 338, 416
AccB7I CCANNNNNTGG 2 cut(s) 673, 1742
AccBSI CCGCTC 2 cut(s) 2041, 2356
AccI GTMKAC 1 cut(s) 1487
AccII CGCG 2 cut(s) 658, 2691
AccIII TCCGGA 1 cut(s) 2337
AclWI GGATC 6 cut(s) 35, 476, 588, 1518, 1531, 2348
AcoI YGGCCR 4 cut(s) 174, 256, 664, 931
AcsI RAATTY 1 cut(s) 2654
AcuI CTGAAG 2 cut(s) 1440, 2660
AcyI GRCGYC 2 cut(s) 113, 152
AfaI GTAC 6 cut(s) 51, 200, 308, 2283, 2688, 2776
AfeI AGCGCT 1 cut(s) 1150
AflIII ACRYGT 3 cut(s) 261, 508, 1482
AhlI ACTAGT 1 cut(s) 1124
AjiI CACGTC 1 cut(s) 7
Alw21I GWGCWC 2 cut(s) 65, 435
Alw26I GTCTC 6 cut(s) 150, 967, 1161, 1473, 1778, 2196
Alw44I GTGCAC 1 cut(s) 431
AlwI GGATC 6 cut(s) 35, 476, 588, 1518, 1531, 2348
AlwNI CAGNNNCTG 4 cut(s) 1430, 1580, 1787, 2917
Aor13HI TCCGGA 1 cut(s) 2337
Aor51HI AGCGCT 1 cut(s) 1150
ApaI GGGCCC 1 cut(s) 1033
ApaLI GTGCAC 1 cut(s) 431
ApoI RAATTY 1 cut(s) 2654
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AseI ATTAAT 2 cut(s) 1920, 2319
AspA2I CCTAGG 2 cut(s) 1033, 2197
AspLEI GCGC 8 cut(s) 115, 133, 660, 1047, 1151, 1880, 2133, 2693
AsuC2I CCSGG 7 cut(s) 125, 243, 319, 1093, 1871, 2167, 2975
AsuHPI GGTGA 7 cut(s) 1232, 1822, 2111, 2145, 2375, 2859, 2871
AvaII GGWCC 7 cut(s) 179, 314, 557, 647, 806, 1866, 2615
AvrII CCTAGG 2 cut(s) 1033, 2197
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 4 cut(s) 435, 751, 1033, 1932
BalI TGGCCA 1 cut(s) 258
BamHI GGATCC 1 cut(s) 1523
BanI GGYRCC 4 cut(s) 22, 112, 338, 416
BanII GRGCYC 4 cut(s) 1033, 1664, 2083, 2981
BauI CACGAG 1 cut(s) 1962
BbsI GAAGAC 1 cut(s) 845
Bbv12I GWGCWC 2 cut(s) 65, 435
BbvCI CCTCAGC 2 cut(s) 576, 1581
BceAI ACGGC 7 cut(s) 81, 626, 679, 743, 918, 1205, 2937
BcgI CGANNNNNNTGC 4 cut(s) 596, 630, 2303, 2337
BciVI GTATCC 1 cut(s) 1075
BclI TGATCA 1 cut(s) 1701
BcnI CCSGG 7 cut(s) 125, 243, 319, 1093, 1871, 2167, 2975
BcoDI GTCTC 6 cut(s) 150, 967, 1161, 1473, 1778, 2196
BcuI ACTAGT 1 cut(s) 1124
BfmI CTRYAG 8 cut(s) 522, 1086, 1173, 1314, 1329, 1335, 1779, 1884
BfoI RGCGCY 2 cut(s) 116, 1152
BfuI GTATCC 1 cut(s) 1075
BglI GCCNNNNNGGC 2 cut(s) 619, 1601
BglII AGATCT 3 cut(s) 1288, 2020, 2815
BlnI CCTAGG 2 cut(s) 1033, 2197
BlpI GCTNAGC 1 cut(s) 2037
Bme18I GGWCC 7 cut(s) 179, 314, 557, 647, 806, 1866, 2615
BmgBI CACGTC 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 1797
BmsI GCATC 7 cut(s) 868, 1144, 1328, 1574, 1765, 2398, 2898
BmuI ACTGGG 1 cut(s) 1797
BoxI GACNNNNGTC 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 845
BpmI CTGGAG 5 cut(s) 978, 1044, 1502, 1764, 2492
Bpu10I CCTNAGC 2 cut(s) 576, 1581
Bpu1102I GCTNAGC 1 cut(s) 2037
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 7 cut(s) 125, 243, 319, 1093, 1871, 2167, 2975
BsaAI YACGTR 3 cut(s) 198, 1250, 2904
BsaBI GATNNNNATC 2 cut(s) 39, 1653
BsaHI GRCGYC 2 cut(s) 113, 152
BsaWI WCCGGW 3 cut(s) 169, 793, 2337
BsaXI ACNNNNNCTCC 3 cut(s) 26, 2193, 2223
Bse118I RCCGGY 2 cut(s) 115, 413
Bse1I ACTGG 5 cut(s) 756, 900, 1281, 1457, 1792
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 2014
Bse8I GATNNNNATC 2 cut(s) 39, 1653
BseAI TCCGGA 1 cut(s) 2337
BseJI GATNNNNATC 2 cut(s) 39, 1653
BseMI GCAATG 1 cut(s) 2014
BseMII CTCAG 8 cut(s) 122, 155, 567, 617, 1358, 1572, 1677, 2028
BseNI ACTGG 5 cut(s) 756, 900, 1281, 1457, 1792
BseRI GAGGAG 3 cut(s) 4, 305, 848
BseSI GKGCMC 4 cut(s) 435, 751, 1033, 1932
BseYI CCCAGC 1 cut(s) 1718
Bsh1236I CGCG 2 cut(s) 658, 2691
Bsh1285I CGRYCG 2 cut(s) 276, 2541
BshNI GGYRCC 4 cut(s) 22, 112, 338, 416
BsiEI CGRYCG 2 cut(s) 276, 2541
BsiHKAI GWGCWC 2 cut(s) 65, 435
BslFI GGGAC 3 cut(s) 388, 1879, 2748
BsmAI GTCTC 6 cut(s) 150, 967, 1161, 1473, 1778, 2196
BsmBI CGTCTC 1 cut(s) 1161
BsmFI GGGAC 3 cut(s) 388, 1879, 2748
BsmI GAATGC 2 cut(s) 2351, 2926
Bsp120I GGGCCC 1 cut(s) 1029
Bsp1286I GDGCHC 8 cut(s) 65, 435, 751, 1033, 1664, 1932, 2083, 2981
Bsp13I TCCGGA 1 cut(s) 2337
Bsp1407I TGTACA 2 cut(s) 49, 2281
Bsp1720I GCTNAGC 1 cut(s) 2037
BspCNI CTCAG 8 cut(s) 121, 154, 568, 616, 1357, 1573, 1676, 2029
BspEI TCCGGA 1 cut(s) 2337
BspFNI CGCG 2 cut(s) 658, 2691
BspHI TCATGA 2 cut(s) 2302, 2695
BspMAI CTGCAG 7 cut(s) 1090, 1177, 1318, 1333, 1339, 1783, 1888
BspPI GGATC 6 cut(s) 35, 476, 588, 1518, 1531, 2348
BspT107I GGYRCC 4 cut(s) 22, 112, 338, 416
BsrBI CCGCTC 2 cut(s) 2041, 2356
BsrDI GCAATG 1 cut(s) 2014
BsrFI RCCGGY 2 cut(s) 115, 413
BsrGI TGTACA 2 cut(s) 49, 2281
BsrI ACTGG 5 cut(s) 756, 900, 1281, 1457, 1792
BssAI RCCGGY 2 cut(s) 115, 413
BssNAI GTATAC 1 cut(s) 1488
BssNI GRCGYC 2 cut(s) 113, 152
BssSI CACGAG 1 cut(s) 1962
BssT1I CCWWGG 4 cut(s) 1033, 1130, 1611, 2197
Bst1107I GTATAC 1 cut(s) 1488
Bst2BI CACGAG 1 cut(s) 1962
Bst4CI ACNGT 5 cut(s) 437, 887, 1241, 2402, 2771
Bst6I CTCTTC 2 cut(s) 2198, 2250
BstACI GRCGYC 2 cut(s) 113, 152
BstAPI GCANNNNNTGC 4 cut(s) 1325, 1414, 1592, 2786
BstAUI TGTACA 2 cut(s) 49, 2281
BstBAI YACGTR 3 cut(s) 198, 1250, 2904
BstDEI CTNAG 9 cut(s) 108, 141, 576, 603, 1344, 1581, 1663, 2037, 2075
BstENI CCTNNNNNAGG 1 cut(s) 648
BstFNI CGCG 2 cut(s) 658, 2691
BstH2I RGCGCY 2 cut(s) 116, 1152
BstHHI GCGC 8 cut(s) 115, 133, 660, 1047, 1151, 1880, 2133, 2693
BstMAI GTCTC 6 cut(s) 150, 967, 1161, 1473, 1778, 2196
BstMCI CGRYCG 2 cut(s) 276, 2541
BstNSI RCATGY 7 cut(s) 191, 512, 823, 1019, 1205, 1328, 1486
BstPAI GACNNNNGTC 1 cut(s) 272
BstSFI CTRYAG 8 cut(s) 522, 1086, 1173, 1314, 1329, 1335, 1779, 1884
BstSLI GKGCMC 4 cut(s) 435, 751, 1033, 1932
BstUI CGCG 2 cut(s) 658, 2691
BstV2I GAAGAC 1 cut(s) 845
BstX2I RGATCY 5 cut(s) 580, 1288, 1523, 2020, 2815
BstXI CCANNNNNNTGG 1 cut(s) 777
BstYI RGATCY 5 cut(s) 580, 1288, 1523, 2020, 2815
BstZ17I GTATAC 1 cut(s) 1488
Bsu36I CCTNAGG 1 cut(s) 108
BsuI GTATCC 1 cut(s) 1075
BtgZI GCGATG 1 cut(s) 1305
BtrI CACGTC 1 cut(s) 7
BtsIMutI CAGTG 6 cut(s) 433, 749, 892, 893, 1500, 1597
CaiI CAGNNNCTG 4 cut(s) 1430, 1580, 1787, 2917
CciI TCATGA 2 cut(s) 2302, 2695
CfoI GCGC 8 cut(s) 115, 133, 660, 1047, 1151, 1880, 2133, 2693
Cfr10I RCCGGY 2 cut(s) 115, 413
CpoI CGGWCCG 1 cut(s) 179
CseI GACGC 3 cut(s) 141, 1178, 2501
CsiI ACCWGGT 1 cut(s) 1360
Csp6I GTAC 6 cut(s) 50, 199, 307, 2282, 2687, 2775
CspCI CAANNNNNGTGG 4 cut(s) 331, 366, 2752, 2787
CspI CGGWCCG 1 cut(s) 179
CviQI GTAC 6 cut(s) 50, 199, 307, 2282, 2687, 2775
DdeI CTNAG 9 cut(s) 108, 141, 576, 603, 1344, 1581, 1663, 2037, 2075
DinI GGCGCC 1 cut(s) 114
DraI TTTAAA 1 cut(s) 1956
DrdI GACNNNNNNGTC 1 cut(s) 382
DseDI GACNNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 4 cut(s) 174, 256, 664, 931
Eam1104I CTCTTC 2 cut(s) 2198, 2250
EarI CTCTTC 2 cut(s) 2198, 2250
Eco130I CCWWGG 4 cut(s) 1033, 1130, 1611, 2197
Eco24I GRGCYC 4 cut(s) 1033, 1664, 2083, 2981
Eco32I GATATC 2 cut(s) 695, 980
Eco47I GGWCC 7 cut(s) 179, 314, 557, 647, 806, 1866, 2615
Eco47III AGCGCT 1 cut(s) 1150
Eco57I CTGAAG 2 cut(s) 1440, 2660
Eco81I CCTNAGG 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 5 cut(s) 641, 1029, 1030, 1866, 2615
EcoRI GAATTC 1 cut(s) 2654
EcoRV GATATC 2 cut(s) 695, 980
EcoT14I CCWWGG 4 cut(s) 1033, 1130, 1611, 2197
EcoT22I ATGCAT 3 cut(s) 821, 2351, 2460
EcoT38I GRGCYC 4 cut(s) 1033, 1664, 2083, 2981
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
ErhI CCWWGG 4 cut(s) 1033, 1130, 1611, 2197
Esp3I CGTCTC 1 cut(s) 1161
FaqI GGGAC 3 cut(s) 388, 1879, 2748
FauI CCCGC 2 cut(s) 1673, 1845
FauNDI CATATG 2 cut(s) 772, 907
FbaI TGATCA 1 cut(s) 1701
FblI GTMKAC 1 cut(s) 1487
FriOI GRGCYC 4 cut(s) 1033, 1664, 2083, 2981
GlaI GCGC 8 cut(s) 114, 132, 659, 1046, 1150, 1879, 2132, 2692
GsaI CCCAGC 1 cut(s) 1722
GsuI CTGGAG 5 cut(s) 978, 1044, 1502, 1764, 2492
HaeII RGCGCY 2 cut(s) 116, 1152
HgaI GACGC 3 cut(s) 141, 1178, 2501
HhaI GCGC 8 cut(s) 115, 133, 660, 1047, 1151, 1880, 2133, 2693
Hin1I GRCGYC 2 cut(s) 113, 152
Hin6I GCGC 8 cut(s) 113, 131, 658, 1045, 1149, 1878, 2131, 2691
HinP1I GCGC 8 cut(s) 113, 131, 658, 1045, 1149, 1878, 2131, 2691
HincII GTYRAC 3 cut(s) 267, 1185, 2212
HindII GTYRAC 3 cut(s) 267, 1185, 2212
HinfI GANTC 8 cut(s) 11, 166, 325, 376, 960, 1058, 1386, 2503
HphI GGTGA 7 cut(s) 1232, 1822, 2111, 2145, 2375, 2859, 2871
Hpy188I TCNGA 4 cut(s) 847, 1666, 2566, 2832
Hpy188III TCNNGA 8 cut(s) 38, 593, 1023, 1430, 2089, 2303, 2338, 2696
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 6 cut(s) 223, 978, 1838, 2166, 2254, 2585
HpyCH4III ACNGT 5 cut(s) 437, 887, 1241, 2402, 2771
HpyCH4IV ACGT 5 cut(s) 6, 197, 263, 1249, 2903
HpyF3I CTNAG 9 cut(s) 108, 141, 576, 603, 1344, 1581, 1663, 2037, 2075
HpySE526I ACGT 5 cut(s) 6, 197, 263, 1249, 2903
Hsp92I GRCGYC 2 cut(s) 113, 152
HspAI GCGC 8 cut(s) 113, 131, 658, 1045, 1149, 1878, 2131, 2691
KasI GGCGCC 1 cut(s) 112
KflI GGGWCCC 1 cut(s) 1866
Kpn2I TCCGGA 1 cut(s) 2337
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 1701
LmnI GCTCC 5 cut(s) 958, 1432, 1521, 1793, 2086
LweI GCATC 7 cut(s) 868, 1144, 1328, 1574, 1765, 2398, 2898
MabI ACCWGGT 1 cut(s) 1360
MaeII ACGT 5 cut(s) 6, 197, 263, 1249, 2903
MaeIII GTNAC 4 cut(s) 1120, 1220, 1260, 2626
MbiI CCGCTC 2 cut(s) 2041, 2356
MfeI CAATTG 2 cut(s) 948, 2410
MflI RGATCY 5 cut(s) 580, 1288, 1523, 2020, 2815
MhlI GDGCHC 8 cut(s) 65, 435, 751, 1033, 1664, 1932, 2083, 2981
MlsI TGGCCA 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 258
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 4 cut(s) 5, 160, 370, 969
Mox20I TGGCCA 1 cut(s) 258
Mph1103I ATGCAT 3 cut(s) 821, 2351, 2460
MroI TCCGGA 1 cut(s) 2337
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 258
MseI TTAA 6 cut(s) 1002, 1626, 1920, 1955, 2288, 2319
MslI CAYNNNNRTG 6 cut(s) 438, 775, 814, 927, 2453, 2700
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 9 cut(s) 281, 389, 425, 761, 1160, 1208, 1334, 1340, 1742
MunI CAATTG 2 cut(s) 948, 2410
Mva1269I GAATGC 2 cut(s) 2351, 2926
MvnI CGCG 2 cut(s) 658, 2691
NaeI GCCGGC 1 cut(s) 117
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 7 cut(s) 125, 243, 319, 1093, 1871, 2167, 2975
NdeI CATATG 2 cut(s) 772, 907
NgoMIV GCCGGC 1 cut(s) 115
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 1220
NsiI ATGCAT 3 cut(s) 821, 2351, 2460
NspI RCATGY 7 cut(s) 191, 512, 823, 1019, 1205, 1328, 1486
PaeI GCATGC 3 cut(s) 191, 1205, 1328
PagI TCATGA 2 cut(s) 2302, 2695
PasI CCCWGGG 1 cut(s) 2731
PciI ACATGT 2 cut(s) 508, 1482
PcsI WCGNNNNNNNCGW 1 cut(s) 2398
PctI GAATGC 2 cut(s) 2351, 2926
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 325, 1058, 1386, 2503
PflMI CCANNNNNTGG 2 cut(s) 673, 1742
PfoI TCCNGGA 3 cut(s) 955, 1526, 2617
Ple19I CGATCG 1 cut(s) 2541
PleI GAGTC 4 cut(s) 5, 160, 370, 968
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 4 cut(s) 5, 160, 370, 968
Ppu21I YACGTR 3 cut(s) 198, 1250, 2904
PpuMI RGGWCCY 2 cut(s) 1866, 2615
PscI ACATGT 2 cut(s) 508, 1482
PshAI GACNNNNGTC 1 cut(s) 272
PshBI ATTAAT 2 cut(s) 1920, 2319
Psp5II RGGWCCY 2 cut(s) 1866, 2615
PspFI CCCAGC 1 cut(s) 1718
PspOMI GGGCCC 1 cut(s) 1029
PspPPI RGGWCCY 2 cut(s) 1866, 2615
PstI CTGCAG 7 cut(s) 1090, 1177, 1318, 1333, 1339, 1783, 1888
PstNI CAGNNNCTG 4 cut(s) 1430, 1580, 1787, 2917
PsuI RGATCY 5 cut(s) 580, 1288, 1523, 2020, 2815
PvuI CGATCG 1 cut(s) 2541
PvuII CAGCTG 8 cut(s) 389, 425, 761, 1160, 1208, 1334, 1340, 1742
RsaI GTAC 6 cut(s) 51, 200, 308, 2283, 2688, 2776
RsaNI GTAC 6 cut(s) 50, 199, 307, 2282, 2687, 2775
RseI CAYNNNNRTG 6 cut(s) 438, 775, 814, 927, 2453, 2700
Rsr2I CGGWCCG 1 cut(s) 179
RsrII CGGWCCG 1 cut(s) 179
SaqAI TTAA 6 cut(s) 1002, 1626, 1920, 1955, 2288, 2319
SchI GAGTC 4 cut(s) 5, 160, 370, 969
SduI GDGCHC 8 cut(s) 65, 435, 751, 1033, 1664, 1932, 2083, 2981
SexAI ACCWGGT 1 cut(s) 1360
SfaNI GCATC 7 cut(s) 868, 1144, 1328, 1574, 1765, 2398, 2898
SfcI CTRYAG 8 cut(s) 522, 1086, 1173, 1314, 1329, 1335, 1779, 1884
SfoI GGCGCC 1 cut(s) 114
SinI GGWCC 7 cut(s) 179, 314, 557, 647, 806, 1866, 2615
SmiMI CAYNNNNRTG 6 cut(s) 438, 775, 814, 927, 2453, 2700
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
SpeI ACTAGT 1 cut(s) 1124
SphI GCATGC 3 cut(s) 191, 1205, 1328
SspDI GGCGCC 1 cut(s) 112
SspI AATATT 1 cut(s) 1732
StyI CCWWGG 4 cut(s) 1033, 1130, 1611, 2197
TaaI ACNGT 5 cut(s) 437, 887, 1241, 2402, 2771
TaiI ACGT 5 cut(s) 9, 200, 266, 1252, 2906
TaqI TCGA 5 cut(s) 616, 1266, 2343, 2392, 2537
TaqII GACCGA 3 cut(s) 196, 2229, 2463
TatI WGTACW 2 cut(s) 49, 2281
TauI GCSGC 8 cut(s) 71, 131, 160, 281, 368, 658, 1214, 2429
TfiI GAWTC 4 cut(s) 325, 1058, 1386, 2503
Tru1I TTAA 6 cut(s) 1002, 1626, 1920, 1955, 2288, 2319
Tru9I TTAA 6 cut(s) 1002, 1626, 1920, 1955, 2288, 2319
TscAI CASTG 6 cut(s) 440, 756, 892, 900, 1507, 1597
TseFI GTSAC 1 cut(s) 1220
Tsp45I GTSAC 1 cut(s) 1220
TspDTI ATGAA 9 cut(s) 575, 789, 1050, 1378, 1857, 2025, 2319, 2712, 2831
TspRI CASTG 6 cut(s) 440, 756, 892, 900, 1507, 1597
Van91I CCANNNNNTGG 2 cut(s) 673, 1742
VneI GTGCAC 1 cut(s) 431
VpaK11BI GGWCC 7 cut(s) 179, 314, 557, 647, 806, 1866, 2615
VspI ATTAAT 2 cut(s) 1920, 2319
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 1 cut(s) 2654
XceI RCATGY 7 cut(s) 191, 512, 823, 1019, 1205, 1328, 1486
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmaJI CCTAGG 2 cut(s) 1033, 2197
XmiI GTMKAC 1 cut(s) 1487
Zsp2I ATGCAT 3 cut(s) 821, 2351, 2460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.