Rw5G029900

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002717
Physical Location & Seq
Reverse (-)
46741086 .. 46752770
11685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029900.1

Sequence Viewer

Length: 2991 bp
ATGGATGTGGACTCCTCGATGGCGCCGGAGGCCGATCACGATCCGGCTGTGCAGAACCAGAGCACGGCGGCAGCAGCAGTGGAGGGAGAGCAGCTAGGAGAACAGCCTCAGTCGCCGGCGACGCAACAGCCTCAGTCGCCGGCGTCGCCGCAGTCGCAGCAGCAGCAGCAGCAGCAGACTCCGGTGGCCGGGCCGCGGCATGCCCCGACCTACACGGTGGTCAATGCAATTTTAGAGAAGAAGGAGGACGGGCCAGGCCCGAGGTGTGGCCATACGTTGACTGCGGTTGCCGCTGTTGGCGAGGAGGGCAGTCCTGGGTACATTGGCCCCAGGCTGATATTGTTCGGCGGTGCCACCGCCCTTGAGGGCAATTCTGCGGCTTCAGGGACTCCATCTTCAGCTGGAAGTGCCGGAATAAGACTAGCTGGCGCCACAGCTGATGTGCACTGTTATGACATCCTAACAAATAAATGGTCTAGGATCACTCCCTTTGGAGAACCACCAACACCAAGGGCAGCACATGTAGCAACTGCTGTAGGAACTATGGTTGTTATTCAGGGAGGAATTGGTCCTGCTGGTTTGTCTGCTGAAGATTTGCATGTTCTTGACCTCACTCAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCAGGGCCACGCTACGGACATGTGATGGCATTGGTGGGGCAAAGATATCTCATGGCAATTGGTGGGAATGATGGGAAACGGCCTTTAGCTGATGTTTGGGCGCTGGACACAGCTGCCAAGCCATATGAATGGCGTAAGTTGGAACCAGAGGGGGAGGGTCCACCACCATGCATGTATGCAACTGCAAGTGCAAGGTCGGATGGTCTTCTCCTCCTTTGTGGAGGGAGGGATGCAAACAGTGTGCCATTGGCAAGTGCATATGGCCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCCCCTTCTCCCAGATATCAACATGCAGCAGTTTTTGTTAATGCACGCTTGCATGTTTCTGGAGGGGCCCTTGGTGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCCGGGGTTTGGTGTGATACAAAATCTGTTGTCACGAGTCCAAGGACAGGTAGATACAGCGCTGATGCAGCTGGTGGAGATGCTGCAGTTGAATTGACACGGCGTTGCAGGCATGCAGCTGCCGCGGTTGGTGACCTGATATTTATATACGGTGGTCTACGTGGTGGGGTGCTGCTTGATGACTTACTGGTTGCGGAAGATCTGGCTGCTGCAGAAACAACAACTGCAGCCTCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGCCGTTTGCCTGGAAGATATGGATTCATTGATGAAAGAACAAGGCAGATAATGCCTGAAGCAGCACCTGATGGTGCTGTTGTGCTGGGAAGTCCAGTTGCCCCACCTGTAAATGGAGACATGTATACTGATATAAGCACCGAAAATGCCTTGCTCCAGGGATCCAGGAGACTGAGCAAAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGTGCTACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTAGAATTACCTGATAGAGATCGTGGCTCAGAGGCTACCCCGAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCGTCAGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGGGGAGCTTTGGGCGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCGGAGAGTGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCCAAAAAGGTAATAGCACATCTTTTAAAGCCACGTGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTCTGTGATAGTGCTGAACGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATATTTGGTGATTTACACGGGCAATTTGGCGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACTGCTGGGGATATCGCATATATCGACTATCTCTTCCTAGGAGACTATGTTGACCGAGGCCAACATAGCTTGGAAACCATTTCTCTTCTGCTTGCTTTGAAGGTTGAATATTCCCAAAATGTACACTTAATTAGGGGTAATCATGAAGCTGCAGATATTAATGCTCTTTTTGGCTTTCGGATCGAATGCATTGAGCGAATGGGTGAGAGAGACGGAATATGGGCATGGCATCGAATAAACCGTTTGTTCAATTGGCTTCCTCTAGCTGCTCTAATTGAGAAGAAAATCATTTGTATGCATGGTGGTATTGGTCGGTCAATAAATCATGTTGAACAGATTGAGAATCTCCAGCGTCCTATTACAATGGAAGCAGGCTCAATCGTTCTTATGGATTTATTATGGTCTGACCCAACAGAAAATGATAGTGTGGAAGGATTGCGACCAAATGCTAGGGGTCCTGGATTGGTTACTTTTGGGCCTGATCGTGTCATGGAATTCTGCAACAACAATGATCTTCAGTTGATTGTACGCGCTCATGAATGTGTAATGGATGGCTTTGAACGATTTGCCCAGGGACATTTGATTACACTTTTCTCTGCTACCAATTACTGTGGTACAGCAAATAATGCAGGGGCAATCCTTGTTTTAGGTAGAGATCTTGTGGTGGTCCCGAAACTCATTCATCCCTTACCACCAGCAATTTCTTCACCAGAAACATCGCCCGAACGACATATTGAAGATACGTGGATGCAGCCTCCAACACCAACTAGAGGCCGTCCACAAGTAGCAAATGACCGGGGATCCCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

996

Amino Acids

106.22

Weight (kDa)

5.74

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 79 - 294 1.2e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 81 - 258 4.6e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 143 - 419 1.6e-10 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 248 - 384 3.9e-12 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 263 - 429 8.4e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 329 - 376 5.9e-07 Kelch motif
Metallophos PF00149 699 - 906 8e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 22, 350, 428
AccBSI CCGCTC 1 cut(s) 1692
AccI GTMKAC 2 cut(s) 1258, 1499
AccII CGCG 3 cut(s) 196, 1226, 2703
AclWI GGATC 7 cut(s) 35, 488, 1530, 1543, 2360, 2967, 2980
AcoI YGGCCR 3 cut(s) 186, 268, 943
AcsI RAATTY 1 cut(s) 2666
AcuI CTGAAG 5 cut(s) 366, 381, 609, 1452, 2672
AcvI CACGTG 1 cut(s) 1976
AcyI GRCGYC 3 cut(s) 23, 143, 429
AfaI GTAC 4 cut(s) 320, 2295, 2700, 2788
AfeI AGCGCT 1 cut(s) 1162
AflIII ACRYGT 3 cut(s) 520, 679, 1494
Alw21I GWGCWC 2 cut(s) 65, 447
Alw26I GTCTC 6 cut(s) 979, 1485, 1537, 1790, 2208, 2376
Alw44I GTGCAC 1 cut(s) 443
AlwI GGATC 7 cut(s) 35, 488, 1530, 1543, 2360, 2967, 2980
AlwNI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
Ama87I CYCGRG 2 cut(s) 259, 1687
Aor51HI AGCGCT 1 cut(s) 1162
ApaI GGGCCC 1 cut(s) 1045
ApaLI GTGCAC 1 cut(s) 443
ApoI RAATTY 1 cut(s) 2666
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AseI ATTAAT 2 cut(s) 1932, 2331
AspA2I CCTAGG 1 cut(s) 2209
AspLEI GCGC 7 cut(s) 25, 431, 763, 1059, 1163, 2145, 2705
AsuC2I CCSGG 4 cut(s) 190, 1105, 1883, 2969
AsuHPI GGTGA 6 cut(s) 1244, 1834, 2123, 2157, 2387, 2871
AvaI CYCGRG 2 cut(s) 259, 1687
AvaII GGWCC 6 cut(s) 569, 659, 818, 1878, 2627, 2839
AvrII CCTAGG 1 cut(s) 2209
BaeGI GKGCMC 3 cut(s) 447, 1045, 1944
BalI TGGCCA 1 cut(s) 270
BamHI GGATCC 2 cut(s) 1535, 2972
BanI GGYRCC 3 cut(s) 22, 350, 428
BanII GRGCYC 2 cut(s) 1045, 2095
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BauI CACGAG 1 cut(s) 1135
BbrPI CACGTG 1 cut(s) 1976
BbsI GAAGAC 1 cut(s) 857
Bbv12I GWGCWC 2 cut(s) 65, 447
BbvCI CCTCAGC 1 cut(s) 1593
BceAI ACGGC 7 cut(s) 81, 638, 755, 930, 1217, 1362, 2931
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciVI GTATCC 1 cut(s) 1087
BclI TGATCA 1 cut(s) 1713
BcnI CCSGG 4 cut(s) 190, 1105, 1883, 2969
BcoDI GTCTC 6 cut(s) 979, 1485, 1537, 1790, 2208, 2376
BfaI CTAG 8 cut(s) 95, 422, 477, 2064, 2210, 2435, 2622, 2940
BfoI RGCGCY 4 cut(s) 26, 432, 764, 1164
BfuI GTATCC 1 cut(s) 1087
BglI GCCNNNNNGGC 1 cut(s) 631
BglII AGATCT 3 cut(s) 1300, 2032, 2827
BlnI CCTAGG 1 cut(s) 2209
Bme18I GGWCC 6 cut(s) 569, 659, 818, 1878, 2627, 2839
BmeT110I CYCGRG 2 cut(s) 259, 1687
BmrI ACTGGG 1 cut(s) 1809
BmsI GCATC 7 cut(s) 880, 1156, 1171, 1586, 1777, 2410, 2910
BmuI ACTGGG 1 cut(s) 1809
BpiI GAAGAC 1 cut(s) 857
BpmI CTGGAG 4 cut(s) 1056, 1514, 1776, 2504
Bpu10I CCTNAGC 1 cut(s) 1593
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 4 cut(s) 190, 1105, 1883, 2969
BsaAI YACGTR 3 cut(s) 1262, 1976, 2916
BsaBI GATNNNNATC 2 cut(s) 39, 1665
BsaHI GRCGYC 3 cut(s) 23, 143, 429
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 2205, 2235
Bse118I RCCGGY 2 cut(s) 115, 139
Bse1I ACTGG 3 cut(s) 1293, 1469, 1804
Bse3DI GCAATG 1 cut(s) 2026
Bse8I GATNNNNATC 2 cut(s) 39, 1665
BseGI GGATG 9 cut(s) 10, 456, 865, 895, 946, 1985, 2728, 2854, 2925
BseJI GATNNNNATC 2 cut(s) 39, 1665
BseMI GCAATG 1 cut(s) 2026
BseMII CTCAG 7 cut(s) 122, 146, 629, 1370, 1538, 1584, 1689
BseNI ACTGG 3 cut(s) 1293, 1469, 1804
BseRI GAGGAG 3 cut(s) 4, 317, 860
BseSI GKGCMC 3 cut(s) 447, 1045, 1944
BseYI CCCAGC 3 cut(s) 1459, 1730, 2177
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 3 cut(s) 196, 1226, 2703
BshNI GGYRCC 3 cut(s) 22, 350, 428
BsiHKAI GWGCWC 2 cut(s) 65, 447
BsiHKCI CYCGRG 2 cut(s) 259, 1687
BslFI GGGAC 4 cut(s) 400, 1891, 2760, 2825
BsmAI GTCTC 6 cut(s) 979, 1485, 1537, 1790, 2208, 2376
BsmBI CGTCTC 1 cut(s) 2376
BsmFI GGGAC 4 cut(s) 400, 1891, 2760, 2825
BsmI GAATGC 1 cut(s) 2363
BsoBI CYCGRG 2 cut(s) 259, 1687
Bsp120I GGGCCC 1 cut(s) 1041
Bsp1286I GDGCHC 5 cut(s) 65, 447, 1045, 1944, 2095
Bsp1407I TGTACA 1 cut(s) 2293
BspCNI CTCAG 7 cut(s) 121, 145, 628, 1369, 1539, 1585, 1688
BspFNI CGCG 3 cut(s) 196, 1226, 2703
BspHI TCATGA 2 cut(s) 2314, 2707
BspMAI CTGCAG 9 cut(s) 1102, 1189, 1315, 1330, 1345, 1351, 1795, 1900, 2326
BspPI GGATC 7 cut(s) 35, 488, 1530, 1543, 2360, 2967, 2980
BspT107I GGYRCC 3 cut(s) 22, 350, 428
BsrBI CCGCTC 1 cut(s) 1692
BsrDI GCAATG 1 cut(s) 2026
BsrFI RCCGGY 2 cut(s) 115, 139
BsrGI TGTACA 1 cut(s) 2293
BsrI ACTGG 3 cut(s) 1293, 1469, 1804
BssAI RCCGGY 2 cut(s) 115, 139
BssNAI GTATAC 1 cut(s) 1500
BssNI GRCGYC 3 cut(s) 23, 143, 429
BssSI CACGAG 1 cut(s) 1135
BssT1I CCWWGG 5 cut(s) 509, 1045, 1142, 1623, 2209
Bst1107I GTATAC 1 cut(s) 1500
Bst2BI CACGAG 1 cut(s) 1135
Bst4CI ACNGT 6 cut(s) 217, 449, 899, 1253, 2414, 2783
Bst6I CTCTTC 2 cut(s) 2210, 2262
BstACI GRCGYC 3 cut(s) 23, 143, 429
BstAPI GCANNNNNTGC 3 cut(s) 1426, 1604, 2798
BstAUI TGTACA 1 cut(s) 2293
BstBAI YACGTR 3 cut(s) 1262, 1976, 2916
BstDEI CTNAG 8 cut(s) 108, 132, 615, 1356, 1547, 1593, 1675, 2087
BstDSI CCRYGG 2 cut(s) 194, 1224
BstEII GGTNACC 1 cut(s) 1232
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 9 cut(s) 10, 456, 865, 895, 946, 1985, 2728, 2854, 2925
BstFNI CGCG 3 cut(s) 196, 1226, 2703
BstH2I RGCGCY 4 cut(s) 26, 432, 764, 1164
BstHHI GCGC 7 cut(s) 25, 431, 763, 1059, 1163, 2145, 2705
BstMAI GTCTC 6 cut(s) 979, 1485, 1537, 1790, 2208, 2376
BstPI GGTNACC 1 cut(s) 1232
BstSLI GKGCMC 3 cut(s) 447, 1045, 1944
BstUI CGCG 3 cut(s) 196, 1226, 2703
BstV2I GAAGAC 1 cut(s) 857
BstX2I RGATCY 5 cut(s) 1300, 1535, 2032, 2827, 2972
BstXI CCANNNNNNTGG 2 cut(s) 789, 1980
BstYI RGATCY 5 cut(s) 1300, 1535, 2032, 2827, 2972
BstZ17I GTATAC 1 cut(s) 1500
BsuI GTATCC 1 cut(s) 1087
BtgI CCRYGG 2 cut(s) 194, 1224
BtgZI GCGATG 1 cut(s) 2874
BtsCI GGATG 9 cut(s) 10, 456, 865, 895, 946, 1985, 2728, 2854, 2925
BtsI GCAGTG 2 cut(s) 84, 1092
BtsIMutI CAGTG 5 cut(s) 84, 445, 904, 1092, 1609
CaiI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
CciI TCATGA 2 cut(s) 2314, 2707
CfoI GCGC 7 cut(s) 25, 431, 763, 1059, 1163, 2145, 2705
Cfr10I RCCGGY 2 cut(s) 115, 139
Cfr42I CCGCGG 2 cut(s) 197, 1227
CseI GACGC 3 cut(s) 130, 132, 2513
Csp6I GTAC 4 cut(s) 319, 2294, 2699, 2787
CspCI CAANNNNNGTGG 4 cut(s) 343, 378, 2764, 2799
CviQI GTAC 4 cut(s) 319, 2294, 2699, 2787
DdeI CTNAG 8 cut(s) 108, 132, 615, 1356, 1547, 1593, 1675, 2087
DinI GGCGCC 2 cut(s) 24, 430
DraI TTTAAA 1 cut(s) 1968
EaeI YGGCCR 3 cut(s) 186, 268, 943
Eam1104I CTCTTC 2 cut(s) 2210, 2262
EarI CTCTTC 2 cut(s) 2210, 2262
Eco130I CCWWGG 5 cut(s) 509, 1045, 1142, 1623, 2209
Eco24I GRGCYC 2 cut(s) 1045, 2095
Eco32I GATATC 3 cut(s) 707, 992, 2185
Eco47I GGWCC 6 cut(s) 569, 659, 818, 1878, 2627, 2839
Eco47III AGCGCT 1 cut(s) 1162
Eco57I CTGAAG 5 cut(s) 366, 381, 609, 1452, 2672
Eco72I CACGTG 1 cut(s) 1976
Eco88I CYCGRG 2 cut(s) 259, 1687
Eco91I GGTNACC 1 cut(s) 1232
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoO109I RGGNCCY 5 cut(s) 653, 1041, 1042, 1878, 2627
EcoO65I GGTNACC 1 cut(s) 1232
EcoRI GAATTC 1 cut(s) 2666
EcoRV GATATC 3 cut(s) 707, 992, 2185
EcoT14I CCWWGG 5 cut(s) 509, 1045, 1142, 1623, 2209
EcoT22I ATGCAT 3 cut(s) 833, 2363, 2472
EcoT38I GRGCYC 2 cut(s) 1045, 2095
EgeI GGCGCC 2 cut(s) 24, 430
EheI GGCGCC 2 cut(s) 24, 430
ErhI CCWWGG 5 cut(s) 509, 1045, 1142, 1623, 2209
Esp3I CGTCTC 1 cut(s) 2376
FaqI GGGAC 4 cut(s) 400, 1891, 2760, 2825
FauI CCCGC 2 cut(s) 1685, 1857
FauNDI CATATG 2 cut(s) 784, 919
FbaI TGATCA 1 cut(s) 1713
FblI GTMKAC 2 cut(s) 1258, 1499
FokI GGATG 9 cut(s) 17, 443, 872, 902, 953, 1992, 2735, 2841, 2932
FriOI GRGCYC 2 cut(s) 1045, 2095
FspBI CTAG 8 cut(s) 95, 422, 477, 2064, 2210, 2435, 2622, 2940
GlaI GCGC 7 cut(s) 24, 430, 762, 1058, 1162, 2144, 2704
GsaI CCCAGC 3 cut(s) 1463, 1734, 2181
GsuI CTGGAG 4 cut(s) 1056, 1514, 1776, 2504
HaeII RGCGCY 4 cut(s) 26, 432, 764, 1164
HgaI GACGC 3 cut(s) 130, 132, 2513
HhaI GCGC 7 cut(s) 25, 431, 763, 1059, 1163, 2145, 2705
Hin1I GRCGYC 3 cut(s) 23, 143, 429
Hin6I GCGC 7 cut(s) 23, 429, 761, 1057, 1161, 2143, 2703
HinP1I GCGC 7 cut(s) 23, 429, 761, 1057, 1161, 2143, 2703
HincII GTYRAC 2 cut(s) 279, 2224
HindII GTYRAC 2 cut(s) 279, 2224
HinfI GANTC 7 cut(s) 11, 178, 388, 1070, 1138, 1398, 2515
HphI GGTGA 6 cut(s) 1244, 1834, 2123, 2157, 2387, 2871
Hpy188I TCNGA 4 cut(s) 859, 1678, 2352, 2578
Hpy188III TCNNGA 8 cut(s) 38, 605, 1035, 1135, 2101, 2315, 2708, 2842
Hpy99I CGWCG 2 cut(s) 124, 148
HpyAV CCTTC 6 cut(s) 235, 990, 1850, 2178, 2266, 2597
HpyCH4III ACNGT 6 cut(s) 217, 449, 899, 1253, 2414, 2783
HpyCH4IV ACGT 4 cut(s) 275, 1261, 1975, 2915
HpyF3I CTNAG 8 cut(s) 108, 132, 615, 1356, 1547, 1593, 1675, 2087
HpySE526I ACGT 4 cut(s) 275, 1261, 1975, 2915
Hsp92I GRCGYC 3 cut(s) 23, 143, 429
HspAI GCGC 7 cut(s) 23, 429, 761, 1057, 1161, 2143, 2703
KasI GGCGCC 2 cut(s) 22, 428
KflI GGGWCCC 1 cut(s) 1878
KroI GCCGGC 2 cut(s) 115, 139
KroNI GCCGGC 2 cut(s) 117, 141
Ksp22I TGATCA 1 cut(s) 1713
KspI CCGCGG 2 cut(s) 197, 1227
LmnI GCTCC 4 cut(s) 970, 1533, 1805, 2098
LweI GCATC 7 cut(s) 880, 1156, 1171, 1586, 1777, 2410, 2910
MaeI CTAG 8 cut(s) 95, 422, 477, 2064, 2210, 2435, 2622, 2940
MaeII ACGT 4 cut(s) 275, 1261, 1975, 2915
MaeIII GTNAC 3 cut(s) 1132, 1232, 2638
MbiI CCGCTC 1 cut(s) 1692
MfeI CAATTG 3 cut(s) 717, 960, 2422
MflI RGATCY 5 cut(s) 1300, 1535, 2032, 2827, 2972
MhlI GDGCHC 5 cut(s) 65, 447, 1045, 1944, 2095
MlsI TGGCCA 1 cut(s) 270
MluNI TGGCCA 1 cut(s) 270
Mly113I GGCGCC 2 cut(s) 23, 429
MlyI GAGTC 4 cut(s) 5, 172, 382, 1147
MmeI TCCRAC 4 cut(s) 780, 837, 1071, 2954
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 3 cut(s) 833, 2363, 2472
MreI CGCCGGCG 2 cut(s) 115, 139
MroNI GCCGGC 2 cut(s) 115, 139
MscI TGGCCA 1 cut(s) 270
MseI TTAA 6 cut(s) 1014, 1638, 1932, 1967, 2300, 2331
MslI CAYNNNNRTG 7 cut(s) 450, 787, 826, 939, 1978, 2465, 2712
Msp20I TGGCCA 1 cut(s) 270
MunI CAATTG 3 cut(s) 717, 960, 2422
Mva1269I GAATGC 1 cut(s) 2363
MvnI CGCG 3 cut(s) 196, 1226, 2703
NaeI GCCGGC 2 cut(s) 117, 141
NarI GGCGCC 2 cut(s) 23, 429
NciI CCSGG 4 cut(s) 190, 1105, 1883, 2969
NdeI CATATG 2 cut(s) 784, 919
NgoMIV GCCGGC 2 cut(s) 115, 139
NmuCI GTSAC 2 cut(s) 1132, 1232
NsiI ATGCAT 3 cut(s) 833, 2363, 2472
PaeI GCATGC 3 cut(s) 203, 1217, 1340
PagI TCATGA 2 cut(s) 2314, 2707
PasI CCCWGGG 1 cut(s) 2743
PciI ACATGT 3 cut(s) 520, 679, 1494
PcsI WCGNNNNNNNCGW 2 cut(s) 2193, 2410
PctI GAATGC 1 cut(s) 2363
PdiI GCCGGC 2 cut(s) 117, 141
PfeI GAWTC 3 cut(s) 1070, 1398, 2515
PfoI TCCNGGA 2 cut(s) 1538, 2629
PleI GAGTC 4 cut(s) 5, 172, 382, 1146
PluTI GGCGCC 2 cut(s) 26, 432
PmaCI CACGTG 1 cut(s) 1976
PmlI CACGTG 1 cut(s) 1976
PpsI GAGTC 4 cut(s) 5, 172, 382, 1146
Ppu21I YACGTR 3 cut(s) 1262, 1976, 2916
PpuMI RGGWCCY 2 cut(s) 1878, 2627
PscI ACATGT 3 cut(s) 520, 679, 1494
PshBI ATTAAT 2 cut(s) 1932, 2331
Psp5II RGGWCCY 2 cut(s) 1878, 2627
PspCI CACGTG 1 cut(s) 1976
PspEI GGTNACC 1 cut(s) 1232
PspFI CCCAGC 3 cut(s) 1459, 1730, 2177
PspOMI GGGCCC 1 cut(s) 1041
PspPPI RGGWCCY 2 cut(s) 1878, 2627
PstI CTGCAG 9 cut(s) 1102, 1189, 1315, 1330, 1345, 1351, 1795, 1900, 2326
PstNI CAGNNNCTG 3 cut(s) 1442, 1592, 1799
PsuI RGATCY 5 cut(s) 1300, 1535, 2032, 2827, 2972
PvuII CAGCTG 8 cut(s) 401, 437, 773, 1172, 1220, 1346, 1352, 1754
RsaI GTAC 4 cut(s) 320, 2295, 2700, 2788
RsaNI GTAC 4 cut(s) 319, 2294, 2699, 2787
RseI CAYNNNNRTG 7 cut(s) 450, 787, 826, 939, 1978, 2465, 2712
SacII CCGCGG 2 cut(s) 197, 1227
SaqAI TTAA 6 cut(s) 1014, 1638, 1932, 1967, 2300, 2331
SchI GAGTC 4 cut(s) 5, 172, 382, 1147
SduI GDGCHC 5 cut(s) 65, 447, 1045, 1944, 2095
SfaNI GCATC 7 cut(s) 880, 1156, 1171, 1586, 1777, 2410, 2910
SfoI GGCGCC 2 cut(s) 24, 430
Sfr303I CCGCGG 2 cut(s) 197, 1227
SgrAI CRCCGGYG 2 cut(s) 115, 139
SgrBI CCGCGG 2 cut(s) 197, 1227
SinI GGWCC 6 cut(s) 569, 659, 818, 1878, 2627, 2839
SmiMI CAYNNNNRTG 7 cut(s) 450, 787, 826, 939, 1978, 2465, 2712
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SphI GCATGC 3 cut(s) 203, 1217, 1340
SspDI GGCGCC 2 cut(s) 22, 428
SspI AATATT 2 cut(s) 1744, 2282
SspMI CTAG 8 cut(s) 95, 422, 477, 2064, 2210, 2435, 2622, 2940
StyI CCWWGG 5 cut(s) 509, 1045, 1142, 1623, 2209
TaaI ACNGT 6 cut(s) 217, 449, 899, 1253, 2414, 2783
TaiI ACGT 4 cut(s) 278, 1264, 1978, 2918
TaqI TCGA 5 cut(s) 17, 628, 2196, 2355, 2404
TaqII GACCGA 2 cut(s) 2241, 2475
TatI WGTACW 1 cut(s) 2293
TauI GCSGC 8 cut(s) 71, 151, 196, 199, 293, 380, 1226, 1818
TfiI GAWTC 3 cut(s) 1070, 1398, 2515
Tru1I TTAA 6 cut(s) 1014, 1638, 1932, 1967, 2300, 2331
Tru9I TTAA 6 cut(s) 1014, 1638, 1932, 1967, 2300, 2331
TscAI CASTG 5 cut(s) 84, 452, 904, 1092, 1609
TseFI GTSAC 2 cut(s) 1132, 1232
Tsp45I GTSAC 2 cut(s) 1132, 1232
TspDTI ATGAA 9 cut(s) 801, 1062, 1390, 1422, 1869, 2037, 2331, 2724, 2843
TspGWI ACGGA 4 cut(s) 690, 1737, 2068, 2400
TspRI CASTG 5 cut(s) 84, 452, 904, 1092, 1609
VneI GTGCAC 1 cut(s) 443
VpaK11BI GGWCC 6 cut(s) 569, 659, 818, 1878, 2627, 2839
VspI ATTAAT 2 cut(s) 1932, 2331
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 1 cut(s) 2666
XmaJI CCTAGG 1 cut(s) 2209
XmiI GTMKAC 2 cut(s) 1258, 1499
XspI CTAG 8 cut(s) 95, 422, 477, 2064, 2210, 2435, 2622, 2940
Zsp2I ATGCAT 3 cut(s) 833, 2363, 2472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.