Rroxscaffold_1G00033170

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002717
Physical Location & Seq
Forward (+)
47765481 .. 47778182
12702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033170.1

Sequence Viewer

Length: 2964 bp
ATGGATGTGGACTCCTCGATGGCGCCGGAGGCCGATCACGATCCGGCTGTGCAGAACCAGAGCACGGCGGCAGCAGCAGTGGAGGGAGAGCAGCTAGGAGAACAGCCTCAGTCGCCGGTTTCCCCGGCTACGCAACAGCCTCAGTCGCCGGCGTCGCCGCAGTCGCAGCAGCAACAGCAGCAGCAGACTCCGGTGGCCGGGCCGCGGCATGCCCCGACCTACACGGTGGTCAATGCAATTTTAGAGAAGAAGGAGGACGGACCAGGCCCGAGGTGTGGCCATACGTTGACTGCGGTCGCCGCTGTTGGCGAGGAGGGCAGTCCTGGATACATTGGCCCCAGGCTGATATTGTTCGGCGGTGCCACCGCCCTTGAGGGAAATTCGGCGGCTTCAGGGACTCCATCTTCAGCTGGAAGTGCCGGAATAAGACTAGCTGGTGCCACAGCTGATGTGCACTGTTATGACATCCTAACAAATAAATGGTCTAGGATCACTCCCTTTGGAGAACCACCAACACCAAGGGCAGCACATGTAGCAACTGCTGTAGGAACTATGGTTGTTATTCAGGGAGGAATTGGTCCTGCTGGTTTGTCTGCTGAAGATCTACATGTTCTTGACCTCACTCAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCAGGGCCACGCTACGGACATGTGATGGCATTGGTGGGGCAAAGATATCTCATGGCAATTGGTGGGAATGATGGTAAACGGCCTTTAGCTGATGTTTGGGCGCTGGACACAGCTGCCAAGCCATATGAATGGCGATTAGAGGATAAAGTGATTTGGTATGCAACTGCAAGTGCAAGGTCGGATGGTCTTCTCCTCCTTTGTGGAGGGAGGGATGCAAACAGTGTGCCATTGGCAAGTGCATATGGCCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCCCCATCTCCCAGATATCAACATGCAGCAGTTTTTGTTAATGCACGCTTGCATGTTTCCGGAGGGGCCCTTGGCGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCCGGGGTTTGGTGCGATACAAAATCTGTTGTCACGAGTCCAAGGACAGGTAGATACAGCGCTGATGCAGCTGGTGGAGATGCTGCAGTTGAATTGACACGGCGTTGCAGGCATGCAGCTGCCGCGGTTGGTGACCTGATATTTATATATGGTGGTCTACGTGGTGGGGTGTTGCTTGATGACCTACTGGTTGCGGAAGATCTGGCAGCTGCAGAAACAACAACTGCAGCCTCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGCCGTTTGCCTGGAAGATATGGATTCATTGATGAAAGAACGAGGCAGATAATGCCTGAAGCAGCACCTGATGGTGCTGTTGTGCTGGGAAGTCCAGTTGCCCCACCTGTAAATGGAGACATGTATACTGATATAAGCACTGAAAATGCCTTGCTCCAGGGATCCAGGAGACTGAGCAAAGGGGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGTGCTACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTAGAGTTACCTGATAGAGATCGTGGCTCAGAGGCTACCCCGAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCGTCAGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGGGGAGCTTTGGGCGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCGGAGAGTGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCTAAAAAGGTGCAGGTAATAGCACATCTTTTAAAGCCACGTGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTTTGTGATAGTGCTGAACGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATATTTGGTGATTTACACGGGCAATTTGGCGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACTGCTGGGGATATCGCAACTGTATTGCGTTTGCTACAGAAGTTCACCATTCACATGCAAAGATATATTGACTATCTCTTCCTAGGAGACTATGTTGACCGAGGCCAACATAGCTTGGAAACCATTTCTCTTCTGCTTGCTTTGAAGGTTGAATATTCCCAAAATGTACACTTAATTAGGGGTAACCATGAAGCTGCAGATATTAATGCTCTTTTTGGCTTTCGGATCGAATGCATTGAGCGAATGGGTGAGAGAGACGGAATATGGGCATGGCATCGAATAAACCGTTTGTTCAATTGGCTTCCTCTAGCTGCTTTAATTGAGAAGAAAATCATTTGTATGCATGGTGGTATTGGTCGGTCTGACCCAACAGAAAATGATAGTGTGGAAGGATTGCGACCAAATGCTAGGGGTCCTGGATTGGTTACTTTTGGGCCTGATCGTGTCATGGAATTCTGCAACAACAATGATCTTCAGTTGATTGTACGCGCTCATGAATGTGTAATGGATGGCTTTGAACGATTTGCCCAGGGACATTTGATTACACTTTTCTCTGCTACCAATTACTGTGGTACAGCAAATAATGCAGGGGCAATCCTTGTTTTAGGTAGAGATCTTGTGGTGGTCCCGAAACTCATTCATCCCTTACCACCAGCAATTTCTTCACCAGAAACATCGCCCGAACGACATATTGAAGATACGTGGATGCAGGAACTGAATGCAAACAGACCTCCAACACCAACTAGAGGCCGTCCACAAGTAGCAAATGACCGGGGATCCCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

987

Amino Acids

105.48

Weight (kDa)

6.06

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 82 - 263 2.1e-24 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 83 - 261 3.4e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 146 - 253 6e-06 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 284 - 436 7.8e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 326 - 373 5.8e-07 Kelch motif
Metallophos PF00149 698 - 891 2.1e-30 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1936
AccB1I GGYRCC 3 cut(s) 22, 359, 437
AccBSI CCGCTC 1 cut(s) 1683
AccI GTMKAC 2 cut(s) 1249, 1490
AccII CGCG 3 cut(s) 205, 1217, 2658
AccIII TCCGGA 1 cut(s) 1025
AclWI GGATC 7 cut(s) 35, 497, 1521, 1534, 2402, 2940, 2953
AcoI YGGCCR 3 cut(s) 195, 277, 934
AcsI RAATTY 2 cut(s) 379, 2621
AcuI CTGAAG 5 cut(s) 375, 390, 618, 1443, 2627
AcvI CACGTG 1 cut(s) 1973
AcyI GRCGYC 2 cut(s) 23, 152
AfaI GTAC 3 cut(s) 2337, 2655, 2743
AfeI AGCGCT 1 cut(s) 1153
AflIII ACRYGT 4 cut(s) 529, 607, 688, 1485
Alw21I GWGCWC 2 cut(s) 65, 456
Alw26I GTCTC 6 cut(s) 970, 1476, 1528, 1781, 2250, 2418
Alw44I GTGCAC 1 cut(s) 452
AlwI GGATC 7 cut(s) 35, 497, 1521, 1534, 2402, 2940, 2953
AlwNI CAGNNNCTG 4 cut(s) 1433, 1583, 1790, 2884
Ama87I CYCGRG 2 cut(s) 268, 1678
Aor13HI TCCGGA 1 cut(s) 1025
Aor51HI AGCGCT 1 cut(s) 1153
ApaI GGGCCC 1 cut(s) 1036
ApaLI GTGCAC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 379, 2621
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AseI ATTAAT 2 cut(s) 1923, 2373
AspA2I CCTAGG 1 cut(s) 2251
AspLEI GCGC 6 cut(s) 25, 772, 1050, 1154, 2142, 2660
AsuC2I CCSGG 5 cut(s) 125, 199, 1096, 1874, 2942
AsuHPI GGTGA 7 cut(s) 1235, 1825, 2120, 2154, 2206, 2429, 2826
AvaI CYCGRG 2 cut(s) 268, 1678
AvaII GGWCC 6 cut(s) 260, 578, 668, 1869, 2582, 2794
AvrII CCTAGG 1 cut(s) 2251
BaeGI GKGCMC 2 cut(s) 456, 1036
BalI TGGCCA 1 cut(s) 279
BamHI GGATCC 2 cut(s) 1526, 2945
BanI GGYRCC 3 cut(s) 22, 359, 437
BanII GRGCYC 2 cut(s) 1036, 2092
BarI GAAGNNNNNNTAC 2 cut(s) 1629, 1661
BauI CACGAG 1 cut(s) 1126
BbrPI CACGTG 1 cut(s) 1973
BbsI GAAGAC 1 cut(s) 848
Bbv12I GWGCWC 2 cut(s) 65, 456
BbvCI CCTCAGC 1 cut(s) 1584
BceAI ACGGC 7 cut(s) 81, 647, 764, 921, 1208, 1353, 2904
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciVI GTATCC 2 cut(s) 320, 1078
BclI TGATCA 1 cut(s) 1704
BcnI CCSGG 5 cut(s) 125, 199, 1096, 1874, 2942
BcoDI GTCTC 6 cut(s) 970, 1476, 1528, 1781, 2250, 2418
BfaI CTAG 8 cut(s) 95, 431, 486, 2061, 2252, 2477, 2577, 2913
BfoI RGCGCY 3 cut(s) 26, 773, 1155
BfuAI ACCTGC 1 cut(s) 1936
BfuI GTATCC 2 cut(s) 320, 1078
BglI GCCNNNNNGGC 1 cut(s) 640
BglII AGATCT 4 cut(s) 601, 1291, 2029, 2782
BlnI CCTAGG 1 cut(s) 2251
Bme18I GGWCC 6 cut(s) 260, 578, 668, 1869, 2582, 2794
BmeT110I CYCGRG 2 cut(s) 268, 1678
BmrI ACTGGG 1 cut(s) 1800
BmsI GCATC 7 cut(s) 871, 1147, 1162, 1577, 1768, 2452, 2865
BmuI ACTGGG 1 cut(s) 1800
BoxI GACNNNNGTC 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 848
BpmI CTGGAG 2 cut(s) 1505, 1767
Bpu10I CCTNAGC 1 cut(s) 1584
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 5 cut(s) 125, 199, 1096, 1874, 2942
BsaAI YACGTR 3 cut(s) 1253, 1973, 2871
BsaBI GATNNNNATC 2 cut(s) 39, 1656
BsaHI GRCGYC 2 cut(s) 23, 152
BsaWI WCCGGW 2 cut(s) 190, 1025
BsaXI ACNNNNNCTCC 2 cut(s) 2247, 2277
Bse118I RCCGGY 2 cut(s) 115, 148
Bse1I ACTGG 3 cut(s) 1284, 1460, 1795
Bse3DI GCAATG 1 cut(s) 2023
Bse8I GATNNNNATC 2 cut(s) 39, 1656
BseAI TCCGGA 1 cut(s) 1025
BseGI GGATG 9 cut(s) 10, 465, 856, 886, 937, 1982, 2683, 2809, 2880
BseJI GATNNNNATC 2 cut(s) 39, 1656
BseMI GCAATG 1 cut(s) 2023
BseMII CTCAG 7 cut(s) 122, 155, 638, 1361, 1529, 1575, 1680
BseNI ACTGG 3 cut(s) 1284, 1460, 1795
BseRI GAGGAG 3 cut(s) 4, 326, 851
BseSI GKGCMC 2 cut(s) 456, 1036
BseYI CCCAGC 3 cut(s) 1450, 1721, 2174
BsgI GTGCAG 2 cut(s) 71, 1964
Bsh1236I CGCG 3 cut(s) 205, 1217, 2658
Bsh1285I CGRYCG 1 cut(s) 297
BshNI GGYRCC 3 cut(s) 22, 359, 437
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 65, 456
BsiHKCI CYCGRG 2 cut(s) 268, 1678
BslFI GGGAC 4 cut(s) 409, 1882, 2715, 2780
BsmAI GTCTC 6 cut(s) 970, 1476, 1528, 1781, 2250, 2418
BsmBI CGTCTC 1 cut(s) 2418
BsmFI GGGAC 4 cut(s) 409, 1882, 2715, 2780
BsmI GAATGC 2 cut(s) 2405, 2893
BsoBI CYCGRG 2 cut(s) 268, 1678
Bsp120I GGGCCC 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 65, 456, 1036, 2092
Bsp13I TCCGGA 1 cut(s) 1025
Bsp1407I TGTACA 1 cut(s) 2335
BspCNI CTCAG 7 cut(s) 121, 154, 637, 1360, 1530, 1576, 1679
BspEI TCCGGA 1 cut(s) 1025
BspFNI CGCG 3 cut(s) 205, 1217, 2658
BspHI TCATGA 1 cut(s) 2662
BspMAI CTGCAG 9 cut(s) 1093, 1180, 1306, 1321, 1336, 1342, 1786, 1891, 2368
BspMI ACCTGC 1 cut(s) 1936
BspPI GGATC 7 cut(s) 35, 497, 1521, 1534, 2402, 2940, 2953
BspT107I GGYRCC 3 cut(s) 22, 359, 437
BsrBI CCGCTC 1 cut(s) 1683
BsrDI GCAATG 1 cut(s) 2023
BsrFI RCCGGY 2 cut(s) 115, 148
BsrGI TGTACA 1 cut(s) 2335
BsrI ACTGG 3 cut(s) 1284, 1460, 1795
BssAI RCCGGY 2 cut(s) 115, 148
BssNAI GTATAC 1 cut(s) 1491
BssNI GRCGYC 2 cut(s) 23, 152
BssSI CACGAG 1 cut(s) 1126
BssT1I CCWWGG 5 cut(s) 518, 1036, 1133, 1614, 2251
Bst1107I GTATAC 1 cut(s) 1491
Bst2BI CACGAG 1 cut(s) 1126
Bst4CI ACNGT 6 cut(s) 226, 458, 890, 2191, 2456, 2738
Bst6I CTCTTC 2 cut(s) 2252, 2304
BstACI GRCGYC 2 cut(s) 23, 152
BstAPI GCANNNNNTGC 3 cut(s) 1417, 1595, 2753
BstAUI TGTACA 1 cut(s) 2335
BstBAI YACGTR 3 cut(s) 1253, 1973, 2871
BstDEI CTNAG 8 cut(s) 108, 141, 624, 1347, 1538, 1584, 1666, 2084
BstDSI CCRYGG 2 cut(s) 203, 1215
BstEII GGTNACC 2 cut(s) 1223, 2351
BstENI CCTNNNNNAGG 1 cut(s) 669
BstF5I GGATG 9 cut(s) 10, 465, 856, 886, 937, 1982, 2683, 2809, 2880
BstFNI CGCG 3 cut(s) 205, 1217, 2658
BstH2I RGCGCY 3 cut(s) 26, 773, 1155
BstHHI GCGC 6 cut(s) 25, 772, 1050, 1154, 2142, 2660
BstMAI GTCTC 6 cut(s) 970, 1476, 1528, 1781, 2250, 2418
BstMCI CGRYCG 1 cut(s) 297
BstPAI GACNNNNGTC 1 cut(s) 293
BstPI GGTNACC 2 cut(s) 1223, 2351
BstSLI GKGCMC 2 cut(s) 456, 1036
BstUI CGCG 3 cut(s) 205, 1217, 2658
BstV2I GAAGAC 1 cut(s) 848
BstX2I RGATCY 6 cut(s) 601, 1291, 1526, 2029, 2782, 2945
BstXI CCANNNNNNTGG 2 cut(s) 798, 1977
BstYI RGATCY 6 cut(s) 601, 1291, 1526, 2029, 2782, 2945
BstZ17I GTATAC 1 cut(s) 1491
BsuI GTATCC 2 cut(s) 320, 1078
BtgI CCRYGG 2 cut(s) 203, 1215
BtgZI GCGATG 1 cut(s) 2829
BtsCI GGATG 9 cut(s) 10, 465, 856, 886, 937, 1982, 2683, 2809, 2880
BtsI GCAGTG 2 cut(s) 84, 1083
BtsIMutI CAGTG 6 cut(s) 84, 454, 895, 1083, 1503, 1600
BveI ACCTGC 1 cut(s) 1936
CaiI CAGNNNCTG 4 cut(s) 1433, 1583, 1790, 2884
CciI TCATGA 1 cut(s) 2662
CfoI GCGC 6 cut(s) 25, 772, 1050, 1154, 2142, 2660
Cfr10I RCCGGY 2 cut(s) 115, 148
Cfr42I CCGCGG 2 cut(s) 206, 1218
CseI GACGC 1 cut(s) 141
Csp6I GTAC 3 cut(s) 2336, 2654, 2742
CspCI CAANNNNNGTGG 4 cut(s) 352, 387, 2719, 2754
CviQI GTAC 3 cut(s) 2336, 2654, 2742
DdeI CTNAG 8 cut(s) 108, 141, 624, 1347, 1538, 1584, 1666, 2084
DinI GGCGCC 1 cut(s) 24
DraI TTTAAA 1 cut(s) 1965
EaeI YGGCCR 3 cut(s) 195, 277, 934
Eam1104I CTCTTC 2 cut(s) 2252, 2304
EarI CTCTTC 2 cut(s) 2252, 2304
Eco130I CCWWGG 5 cut(s) 518, 1036, 1133, 1614, 2251
Eco24I GRGCYC 2 cut(s) 1036, 2092
Eco32I GATATC 3 cut(s) 716, 983, 2182
Eco47I GGWCC 6 cut(s) 260, 578, 668, 1869, 2582, 2794
Eco47III AGCGCT 1 cut(s) 1153
Eco57I CTGAAG 5 cut(s) 375, 390, 618, 1443, 2627
Eco72I CACGTG 1 cut(s) 1973
Eco88I CYCGRG 2 cut(s) 268, 1678
Eco91I GGTNACC 2 cut(s) 1223, 2351
EcoNI CCTNNNNNAGG 1 cut(s) 669
EcoO109I RGGNCCY 5 cut(s) 662, 1032, 1033, 1869, 2582
EcoO65I GGTNACC 2 cut(s) 1223, 2351
EcoRI GAATTC 1 cut(s) 2621
EcoRV GATATC 3 cut(s) 716, 983, 2182
EcoT14I CCWWGG 5 cut(s) 518, 1036, 1133, 1614, 2251
EcoT22I ATGCAT 2 cut(s) 2405, 2514
EcoT38I GRGCYC 2 cut(s) 1036, 2092
EgeI GGCGCC 1 cut(s) 24
EheI GGCGCC 1 cut(s) 24
ErhI CCWWGG 5 cut(s) 518, 1036, 1133, 1614, 2251
Esp3I CGTCTC 1 cut(s) 2418
FaqI GGGAC 4 cut(s) 409, 1882, 2715, 2780
FauI CCCGC 2 cut(s) 1676, 1848
FauNDI CATATG 2 cut(s) 793, 910
FbaI TGATCA 1 cut(s) 1704
FblI GTMKAC 2 cut(s) 1249, 1490
FokI GGATG 9 cut(s) 17, 452, 863, 893, 944, 1989, 2690, 2796, 2887
FriOI GRGCYC 2 cut(s) 1036, 2092
FspBI CTAG 8 cut(s) 95, 431, 486, 2061, 2252, 2477, 2577, 2913
GlaI GCGC 6 cut(s) 24, 771, 1049, 1153, 2141, 2659
GsaI CCCAGC 3 cut(s) 1454, 1725, 2178
GsuI CTGGAG 2 cut(s) 1505, 1767
HaeII RGCGCY 3 cut(s) 26, 773, 1155
HgaI GACGC 1 cut(s) 141
HhaI GCGC 6 cut(s) 25, 772, 1050, 1154, 2142, 2660
Hin1I GRCGYC 2 cut(s) 23, 152
Hin6I GCGC 6 cut(s) 23, 770, 1048, 1152, 2140, 2658
HinP1I GCGC 6 cut(s) 23, 770, 1048, 1152, 2140, 2658
HincII GTYRAC 2 cut(s) 288, 2266
HindII GTYRAC 2 cut(s) 288, 2266
HinfI GANTC 6 cut(s) 11, 187, 397, 1061, 1129, 1389
HphI GGTGA 7 cut(s) 1235, 1825, 2120, 2154, 2206, 2429, 2826
Hpy188I TCNGA 4 cut(s) 850, 1669, 2394, 2533
Hpy188III TCNNGA 7 cut(s) 38, 614, 1026, 1126, 2098, 2663, 2797
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 5 cut(s) 244, 1841, 2175, 2308, 2552
HpyCH4III ACNGT 6 cut(s) 226, 458, 890, 2191, 2456, 2738
HpyCH4IV ACGT 4 cut(s) 284, 1252, 1972, 2870
HpyF3I CTNAG 8 cut(s) 108, 141, 624, 1347, 1538, 1584, 1666, 2084
HpySE526I ACGT 4 cut(s) 284, 1252, 1972, 2870
Hsp92I GRCGYC 2 cut(s) 23, 152
HspAI GCGC 6 cut(s) 23, 770, 1048, 1152, 2140, 2658
KasI GGCGCC 1 cut(s) 22
KflI GGGWCCC 1 cut(s) 1869
Kpn2I TCCGGA 1 cut(s) 1025
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 1704
KspI CCGCGG 2 cut(s) 206, 1218
LmnI GCTCC 4 cut(s) 961, 1524, 1796, 2095
LweI GCATC 7 cut(s) 871, 1147, 1162, 1577, 1768, 2452, 2865
MaeI CTAG 8 cut(s) 95, 431, 486, 2061, 2252, 2477, 2577, 2913
MaeII ACGT 4 cut(s) 284, 1252, 1972, 2870
MaeIII GTNAC 5 cut(s) 1123, 1223, 1644, 2351, 2593
MbiI CCGCTC 1 cut(s) 1683
MfeI CAATTG 3 cut(s) 726, 951, 2464
MflI RGATCY 6 cut(s) 601, 1291, 1526, 2029, 2782, 2945
MhlI GDGCHC 4 cut(s) 65, 456, 1036, 2092
MlsI TGGCCA 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 23
MlyI GAGTC 4 cut(s) 5, 181, 391, 1138
MmeI TCCRAC 3 cut(s) 828, 1062, 2927
Mox20I TGGCCA 1 cut(s) 279
Mph1103I ATGCAT 2 cut(s) 2405, 2514
MreI CGCCGGCG 1 cut(s) 148
MroI TCCGGA 1 cut(s) 1025
MroNI GCCGGC 1 cut(s) 148
MscI TGGCCA 1 cut(s) 279
MseI TTAA 7 cut(s) 1005, 1629, 1923, 1964, 2342, 2373, 2486
MslI CAYNNNNRTG 7 cut(s) 459, 796, 930, 1975, 2222, 2507, 2667
Msp20I TGGCCA 1 cut(s) 279
MunI CAATTG 3 cut(s) 726, 951, 2464
Mva1269I GAATGC 2 cut(s) 2405, 2893
MvnI CGCG 3 cut(s) 205, 1217, 2658
NaeI GCCGGC 1 cut(s) 150
NarI GGCGCC 1 cut(s) 23
NciI CCSGG 5 cut(s) 125, 199, 1096, 1874, 2942
NdeI CATATG 2 cut(s) 793, 910
NgoMIV GCCGGC 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 1123, 1223
NsiI ATGCAT 2 cut(s) 2405, 2514
PaeI GCATGC 3 cut(s) 212, 1208, 1331
PagI TCATGA 1 cut(s) 2662
PasI CCCWGGG 1 cut(s) 2698
PciI ACATGT 4 cut(s) 529, 607, 688, 1485
PcsI WCGNNNNNNNCGW 1 cut(s) 2452
PctI GAATGC 2 cut(s) 2405, 2893
PdiI GCCGGC 1 cut(s) 150
PfeI GAWTC 2 cut(s) 1061, 1389
PfoI TCCNGGA 3 cut(s) 322, 1529, 2584
PleI GAGTC 4 cut(s) 5, 181, 391, 1137
PluTI GGCGCC 1 cut(s) 26
PmaCI CACGTG 1 cut(s) 1973
PmlI CACGTG 1 cut(s) 1973
PpsI GAGTC 4 cut(s) 5, 181, 391, 1137
Ppu21I YACGTR 3 cut(s) 1253, 1973, 2871
PpuMI RGGWCCY 2 cut(s) 1869, 2582
PscI ACATGT 4 cut(s) 529, 607, 688, 1485
PshAI GACNNNNGTC 1 cut(s) 293
PshBI ATTAAT 2 cut(s) 1923, 2373
Psp5II RGGWCCY 2 cut(s) 1869, 2582
PspCI CACGTG 1 cut(s) 1973
PspEI GGTNACC 2 cut(s) 1223, 2351
PspFI CCCAGC 3 cut(s) 1450, 1721, 2174
PspOMI GGGCCC 1 cut(s) 1032
PspPPI RGGWCCY 2 cut(s) 1869, 2582
PstI CTGCAG 9 cut(s) 1093, 1180, 1306, 1321, 1336, 1342, 1786, 1891, 2368
PstNI CAGNNNCTG 4 cut(s) 1433, 1583, 1790, 2884
PsuI RGATCY 6 cut(s) 601, 1291, 1526, 2029, 2782, 2945
PvuII CAGCTG 9 cut(s) 410, 446, 782, 1163, 1211, 1301, 1337, 1343, 1745
RsaI GTAC 3 cut(s) 2337, 2655, 2743
RsaNI GTAC 3 cut(s) 2336, 2654, 2742
RseI CAYNNNNRTG 7 cut(s) 459, 796, 930, 1975, 2222, 2507, 2667
SacII CCGCGG 2 cut(s) 206, 1218
SaqAI TTAA 7 cut(s) 1005, 1629, 1923, 1964, 2342, 2373, 2486
SchI GAGTC 4 cut(s) 5, 181, 391, 1138
SduI GDGCHC 4 cut(s) 65, 456, 1036, 2092
SfaNI GCATC 7 cut(s) 871, 1147, 1162, 1577, 1768, 2452, 2865
SfoI GGCGCC 1 cut(s) 24
Sfr303I CCGCGG 2 cut(s) 206, 1218
SgrAI CRCCGGYG 1 cut(s) 148
SgrBI CCGCGG 2 cut(s) 206, 1218
SinI GGWCC 6 cut(s) 260, 578, 668, 1869, 2582, 2794
SmiMI CAYNNNNRTG 7 cut(s) 459, 796, 930, 1975, 2222, 2507, 2667
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SphI GCATGC 3 cut(s) 212, 1208, 1331
SspDI GGCGCC 1 cut(s) 22
SspI AATATT 2 cut(s) 1735, 2324
SspMI CTAG 8 cut(s) 95, 431, 486, 2061, 2252, 2477, 2577, 2913
StyI CCWWGG 5 cut(s) 518, 1036, 1133, 1614, 2251
TaaI ACNGT 6 cut(s) 226, 458, 890, 2191, 2456, 2738
TaiI ACGT 4 cut(s) 287, 1255, 1975, 2873
TaqI TCGA 4 cut(s) 17, 637, 2397, 2446
TaqII GACCGA 2 cut(s) 2283, 2517
TatI WGTACW 1 cut(s) 2335
TauI GCSGC 8 cut(s) 71, 160, 205, 208, 302, 389, 1217, 1809
TfiI GAWTC 2 cut(s) 1061, 1389
Tru1I TTAA 7 cut(s) 1005, 1629, 1923, 1964, 2342, 2373, 2486
Tru9I TTAA 7 cut(s) 1005, 1629, 1923, 1964, 2342, 2373, 2486
TscAI CASTG 6 cut(s) 84, 461, 895, 1083, 1510, 1600
TseFI GTSAC 2 cut(s) 1123, 1223
Tsp45I GTSAC 2 cut(s) 1123, 1223
TspDTI ATGAA 9 cut(s) 810, 1053, 1381, 1413, 1860, 2034, 2373, 2679, 2798
TspGWI ACGGA 5 cut(s) 273, 699, 1728, 2065, 2442
TspRI CASTG 6 cut(s) 84, 461, 895, 1083, 1510, 1600
VneI GTGCAC 1 cut(s) 452
VpaK11BI GGWCC 6 cut(s) 260, 578, 668, 1869, 2582, 2794
VspI ATTAAT 2 cut(s) 1923, 2373
XagI CCTNNNNNAGG 1 cut(s) 669
XapI RAATTY 2 cut(s) 379, 2621
XmaJI CCTAGG 1 cut(s) 2251
XmiI GTMKAC 2 cut(s) 1249, 1490
XspI CTAG 8 cut(s) 95, 431, 486, 2061, 2252, 2477, 2577, 2913
Zsp2I ATGCAT 2 cut(s) 2405, 2514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.