pycom03g11040

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002717
Physical Location & Seq
Reverse (-)
10607873 .. 10619248
11376 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g11040.1

Sequence Viewer

Length: 3006 bp
ATGGACGTGGACTCATCGTCGATGGTGCCGGACACCGATCACGATCCGGCAGTGCCAAACCACAGTACGACGTCGTCTCAGGCCGCAGTAGAAGGGGAACAACAGCTTGTCGAGCAGGCGCAACCGGGAACTGGATCTTCTCCGCAGCAGCCTCAGACGGCGGCGGCTCCACAGGCACCACAGACTCCGGTGGCGGGGCCGCGGCACGCTCCAACGTACTCGGTGGTCAATGCAATTTTAGAGAAAAAAGAGGATGGTCCAGGGCCAAGATGTGGCCACACGTTGACTGCGGTGGCGGCTGTGGGCGAGGAGGGCACGCCGGGCTACATTGGCCCCAGGTTGATTTTATTTGGTGGTGCCACAGCTCTTGAGGGCAATTCGGCGGCTTCAGGGACTCCATCGTCAGCTGGAAGTGCCGGCATAAGACTTGCAGGTGCCACAGCGGACGTGCATTGTTATGACGTGCTAACAAATAAATGGTCTAGGATCACACCCTTTGGAGAACCGCCCACCCCAAGGGCTGCACATGTAGCAACAGCTGTAGGAACTATGGTGGTTATTCAGGGTGGAATTGGTCCTGCTGGTTTGTCTGCTGAGGATCTTCATGTTCTTGACCTCACACAGCAACGGCCTCGATGGCACAGGGTTGTTGTTCAAGGCCCTGGACCTGGGCCACGCTATGGACATGTGATGGCATTAGTGGGGCAAAGATATCTTATGGCAATTGGTGGTAATGATGGAAAACGGCCTTTAGCTGATGTTTGGGCTCTCGACACAGCTGCCAAACCTTATGAATGGCGTAAGTTGGAACCAGAGGGGGAGGGTCCACCACCATGCATGTATGCAACTGCAAGTGCAAGGTCTGATGGTCTTCTTCTCCTTTGTGGAGGGAGGGATGCAAATAGTGTGCCATTGGCAAGTGCATATGGTCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTTTCACCTTCTTCAAGATATCAACATGCAGCAGTCTTTGTTAATGCACGTCTGCATGTTTCTGGAGGGGCCCTTGGTGGTGGCCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCAGGAGTTTGGTGTGATACAAAATCTGTGGTTACTAGTCCAAGGACAGGTAGATACAGTGCTGATGCAGCCGGTGGAGATGCTGCAGTTGAGCTGACAAGGCGTTGCCGACATGCAGCTGCAGCGGTTGGTGACTTGATCTTTATATATGGTGGTCTACGTGGAGGAGTGTTGCTTGATGACCTACTTGTTGCGGAAGATTTGGCTGCTGCTGAAACAACAACAGCAGCTTCGCATGCTGCAGCTGCAGCTGCTCAATCTGTACAAGGTCGTTTAACTGGAAGGTATGAATTCATTGATGACAGAGAAAGACAGTTGACTGAAGCAGCTCCTGATGGTGCTATTGTGCTGGGAAATCCAGTTGCACCCCCAGTAAATGGTGACATGTATACTGATATAAGTACTGAAAATGCTTTGCTCCAGGGAACCCGGAGATTGAGCAAAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCTGAAGCTGAGGCAATCAGTGCCACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTTGAACTACCTGATAGAGATCGTGGTTCAGAGGCTACCCCCAGTGGGAAGCAGATATCTACCTCGATCAAGCCTGATTCTGCTGGGTCAAACAATATTCCATCAGCTGGAGTGCGACTGCATCACAGAGCTGTCGTTGTAGCTGCAGAGACTGGTGGAGCTTTAGGTGGCATGGTGAGGCAACTTTCAATTGATCAATTTGAAAATGAAGGCAGGCGGGTTAGTTATGGGACTCCAGAGAGTGCAACTGCAGCAAGGAAATTGTTAGATCGACAGATGTCCATAAATAGTGTTCCCAAAAAGGTAGTGGCACATCTTTTAAAGCCTCGTGGGTGGAAGCCTCCAGTTCGCAGACAATTTTTCTTAGATTGTAATGAAATAGCAGATCTTTGTGACAGTGCTGAGCGAATATTCTCTAGTGAACCAAGCGTCTTACAGCTTAGGGCTCCTATCAAGATATTTGGTGATTTACATGGGCAATTTGGTGACCTCATGCGCCTTTTTGACGAGTATGGTTCACCTTCAACGGCTGGAGATATCGCATATATCGACTATCTCTTCTTAGGAGATTATGTTGACCGGGGCCAACATAGCTTGGAAACGATTACTCTTCTGCTTGCTTTGAAGGTTGAGTATCCCCAAAATGTACATTTAATCCGTGGAAACCATGAAGCTGCAGATATAAATGCTCTATTTGGCTTTCGGATTGAATGCATTGAGCGAATGGGTGAGAGAGATGGAATTTGGGCGTGGCATCGAATAAACCGTTTGTTTAATTGGCTTCCACTGGCAGCTCTTATTGAAAAGAAAATCATTTGTATGCATGGCGGTATTGGCCGGTCTATAAATCATGTGGAGCAGATTGAGAATCTCCAGCGTCCTATTACAATGGAAGCTGGCTCCATTGTGCTTATGGATTTGTTATGGTCTGACCCAACAGAAAATGACAGTGTGGAAGGTTTACGACCAAATGCTAGGGGTCCTGGGCTGGTTACATTTGGGCCTGATCGTGTCATGGAATTCTGCAATAACAATGATCTTCAGTTGATTGTGCGTGCACATGAATGTGTAATGGATGGATTTGAGCGTTTTGCCCAGGGGCATCTGATTACACTTTTCTCTGCTACCAATTATTGTGGTACGGCCAATAATGCCGGGGCAATCCTAGTCTTGGGTAGGGATCTTGTTGTGGTTCCGAAACTCATTCATCCCTTACCACCAGCAATTTCATCACCAGAAACTTCACCGGAACGCCATATTGAAGATACATGGATGCAGGAGTTGAATGCAAACAGACCTCCAACACCGACTAGAGGCCGTCCCCAAGGAGCTAATGATCGGGGTTCCCTTGCTTGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_2 Kelch_4 Metallophos

Protein Analysis

1002

Amino Acids

106.65

Weight (kDa)

5.68

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 82 - 296 1.2e-29 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 83 - 260 6.7e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 145 - 423 2.7e-10 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 201 - 421 1.5e-09 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 250 - 386 1.2e-11 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 264 - 387 4.1e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 332 - 378 1.6e-06 Kelch motif
Metallophos PF00149 698 - 905 6.6e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 422
AasI GACNNNNNNGTC 1 cut(s) 400
AatII GACGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 422
AccB1I GGYRCC 4 cut(s) 25, 175, 356, 434
AccB7I CCANNNNNTGG 4 cut(s) 272, 680, 1484, 1751
AccI GTMKAC 2 cut(s) 1264, 1496
AccII CGCG 1 cut(s) 202
AclWI GGATC 5 cut(s) 38, 142, 494, 606, 2832
AcoI YGGCCR 5 cut(s) 274, 949, 1060, 2479, 2787
AcsI RAATTY 3 cut(s) 1397, 2385, 2663
AcuI CTGAAG 4 cut(s) 372, 1449, 1605, 2669
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 6 cut(s) 67, 218, 1371, 1510, 2292, 2785
AflIII ACRYGT 4 cut(s) 279, 526, 685, 1491
AhdI GACNNNNNGTC 1 cut(s) 16
AhlI ACTAGT 1 cut(s) 1142
AjiI CACGTC 4 cut(s) 7, 448, 463, 1028
AjnI CCWGG 8 cut(s) 259, 335, 661, 667, 973, 1527, 2626, 2739
Alw21I GWGCWC 1 cut(s) 2704
Alw26I GTCTC 2 cut(s) 81, 1787
Alw44I GTGCAC 1 cut(s) 2700
AlwI GGATC 5 cut(s) 38, 142, 494, 606, 2832
AlwNI CAGNNNCTG 3 cut(s) 1439, 1583, 1796
ApaI GGGCCC 1 cut(s) 1051
ApaLI GTGCAC 1 cut(s) 2700
ApoI RAATTY 3 cut(s) 1397, 2385, 2663
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AspLEI GCGC 2 cut(s) 121, 2142
AsuC2I CCSGG 5 cut(s) 126, 321, 1537, 2225, 2800
AvaII GGWCC 5 cut(s) 257, 575, 665, 824, 2624
BaeGI GKGCMC 3 cut(s) 317, 1051, 2704
BalI TGGCCA 1 cut(s) 276
BanI GGYRCC 4 cut(s) 25, 175, 356, 434
BanII GRGCYC 3 cut(s) 769, 1051, 2092
BarI GAAGNNNNNNTAC 2 cut(s) 1635, 1667
BauI CACGAG 1 cut(s) 1971
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 2704
BbvCI CCTCAGC 2 cut(s) 594, 1590
BceAI ACGGC 7 cut(s) 174, 644, 761, 936, 2187, 2802, 2946
BcgI CGANNNNNNTGC 4 cut(s) 614, 648, 761, 795
BciT130I CCWGG 8 cut(s) 261, 337, 663, 669, 975, 1529, 2628, 2741
BciVI GTATCC 2 cut(s) 1093, 2289
BclI TGATCA 1 cut(s) 1837
BcnI CCSGG 5 cut(s) 126, 321, 1537, 2225, 2800
BcoDI GTCTC 2 cut(s) 81, 1787
BcuI ACTAGT 1 cut(s) 1142
BfaI CTAG 6 cut(s) 483, 1143, 2061, 2619, 2810, 2955
BfmI CTRYAG 9 cut(s) 540, 1104, 1191, 1227, 1347, 1353, 1788, 1893, 2319
BfuAI ACCTGC 1 cut(s) 422
BfuI GTATCC 2 cut(s) 1093, 2289
BglI GCCNNNNNGGC 2 cut(s) 637, 1610
BglII AGATCT 1 cut(s) 2029
BlpI GCTNAGC 1 cut(s) 2046
BmcAI AGTACT 1 cut(s) 1510
Bme18I GGWCC 5 cut(s) 257, 575, 665, 824, 2624
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgBI CACGTC 4 cut(s) 7, 448, 463, 1028
BmrI ACTGGG 2 cut(s) 1472, 1680
BmsI GCATC 8 cut(s) 886, 1162, 1177, 1583, 1774, 2407, 2755, 2907
BmuI ACTGGG 2 cut(s) 1472, 1680
BoxI GACNNNNGTC 1 cut(s) 1921
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 7 cut(s) 1062, 1511, 1773, 1863, 1971, 2196, 2501
Bpu10I CCTNAGC 3 cut(s) 594, 1590, 2084
Bpu1102I GCTNAGC 1 cut(s) 2046
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 5 cut(s) 126, 321, 1537, 2225, 2800
BsaAI YACGTR 1 cut(s) 1268
BsaBI GATNNNNATC 2 cut(s) 42, 1662
BsaHI GRCGYC 1 cut(s) 71
BsaWI WCCGGW 2 cut(s) 187, 2890
BsaXI ACNNNNNCTCC 2 cut(s) 2202, 2232
Bse118I RCCGGY 3 cut(s) 416, 1178, 2481
Bse1I ACTGG 8 cut(s) 136, 1390, 1466, 1478, 1686, 1801, 1988, 2436
Bse8I GATNNNNATC 2 cut(s) 42, 1662
BseBI CCWGG 8 cut(s) 261, 337, 663, 669, 975, 1529, 2628, 2741
BseGI GGATG 6 cut(s) 259, 901, 952, 2725, 2851, 2922
BseJI GATNNNNATC 2 cut(s) 42, 1662
BseMII CTCAG 5 cut(s) 92, 167, 585, 1581, 2037
BseNI ACTGG 8 cut(s) 136, 1390, 1466, 1478, 1686, 1801, 1988, 2436
BseRI GAGGAG 2 cut(s) 323, 1287
BseSI GKGCMC 3 cut(s) 317, 1051, 2704
BseYI CCCAGC 2 cut(s) 1456, 1727
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 202
BshNI GGYRCC 4 cut(s) 25, 175, 356, 434
BsiHKAI GWGCWC 1 cut(s) 2704
BslFI GGGAC 3 cut(s) 406, 1888, 2949
BsmAI GTCTC 2 cut(s) 81, 1787
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 3 cut(s) 406, 1888, 2949
BsmI GAATGC 2 cut(s) 2360, 2935
Bsp120I GGGCCC 1 cut(s) 1047
Bsp1286I GDGCHC 5 cut(s) 317, 769, 1051, 2092, 2704
Bsp1407I TGTACA 2 cut(s) 1369, 2290
Bsp1720I GCTNAGC 1 cut(s) 2046
BspCNI CTCAG 5 cut(s) 91, 166, 586, 1582, 2038
BspFNI CGCG 1 cut(s) 202
BspMAI CTGCAG 8 cut(s) 1108, 1195, 1231, 1351, 1357, 1792, 1897, 2323
BspMI ACCTGC 1 cut(s) 422
BspPI GGATC 5 cut(s) 38, 142, 494, 606, 2832
BspT107I GGYRCC 4 cut(s) 25, 175, 356, 434
BsrFI RCCGGY 3 cut(s) 416, 1178, 2481
BsrGI TGTACA 2 cut(s) 1369, 2290
BsrI ACTGG 8 cut(s) 136, 1390, 1466, 1478, 1686, 1801, 1988, 2436
BssAI RCCGGY 3 cut(s) 416, 1178, 2481
BssNAI GTATAC 1 cut(s) 1497
BssNI GRCGYC 1 cut(s) 71
BssSI CACGAG 1 cut(s) 1971
BssT1I CCWWGG 5 cut(s) 515, 1051, 1148, 1620, 2968
Bst1107I GTATAC 1 cut(s) 1497
Bst2BI CACGAG 1 cut(s) 1971
Bst2UI CCWGG 8 cut(s) 261, 337, 663, 669, 975, 1529, 2628, 2741
Bst4CI ACNGT 6 cut(s) 65, 1166, 1422, 2042, 2411, 2594
Bst6I CTCTTC 2 cut(s) 2207, 2259
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 2 cut(s) 1369, 2290
BstBAI YACGTR 1 cut(s) 1268
BstC8I GCNNGC 9 cut(s) 117, 207, 317, 418, 1344, 1859, 2262, 2542, 2700
BstDEI CTNAG 8 cut(s) 78, 153, 594, 1590, 2008, 2046, 2084, 2206
BstDSI CCRYGG 2 cut(s) 200, 2302
BstEII GGTNACC 1 cut(s) 2129
BstF5I GGATG 6 cut(s) 259, 901, 952, 2725, 2851, 2922
BstFNI CGCG 1 cut(s) 202
BstHHI GCGC 2 cut(s) 121, 2142
BstMAI GTCTC 2 cut(s) 81, 1787
BstNI CCWGG 8 cut(s) 261, 337, 663, 669, 975, 1529, 2628, 2741
BstNSI RCATGY 8 cut(s) 530, 689, 841, 1007, 1037, 1223, 1346, 1495
BstPAI GACNNNNGTC 1 cut(s) 1921
BstPI GGTNACC 1 cut(s) 2129
BstSFI CTRYAG 9 cut(s) 540, 1104, 1191, 1227, 1347, 1353, 1788, 1893, 2319
BstSLI GKGCMC 3 cut(s) 317, 1051, 2704
BstUI CGCG 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 4 cut(s) 134, 598, 2029, 2824
BstYI RGATCY 4 cut(s) 134, 598, 2029, 2824
BstZ17I GTATAC 1 cut(s) 1497
BsuI GTATCC 2 cut(s) 1093, 2289
BtgI CCRYGG 2 cut(s) 200, 2302
BtrI CACGTC 4 cut(s) 7, 448, 463, 1028
BtsCI GGATG 6 cut(s) 259, 901, 952, 2725, 2851, 2922
BtsI GCAGTG 2 cut(s) 57, 1098
BtsIMutI CAGTG 8 cut(s) 57, 1098, 1171, 1606, 1693, 2047, 2429, 2599
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 9 cut(s) 117, 207, 317, 418, 1344, 1859, 2262, 2542, 2700
CaiI CAGNNNCTG 3 cut(s) 1439, 1583, 1796
CfoI GCGC 2 cut(s) 121, 2142
Cfr10I RCCGGY 3 cut(s) 416, 1178, 2481
Cfr42I CCGCGG 1 cut(s) 203
CseI GACGC 2 cut(s) 2062, 2510
Csp6I GTAC 6 cut(s) 66, 217, 1370, 1509, 2291, 2784
CspCI CAANNNNNGTGG 6 cut(s) 349, 384, 1116, 1151, 2761, 2796
CviQI GTAC 6 cut(s) 66, 217, 1370, 1509, 2291, 2784
DdeI CTNAG 8 cut(s) 78, 153, 594, 1590, 2008, 2046, 2084, 2206
DraI TTTAAA 1 cut(s) 1965
DrdI GACNNNNNNGTC 1 cut(s) 400
DriI GACNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 5 cut(s) 274, 949, 1060, 2479, 2787
Eam1104I CTCTTC 2 cut(s) 2207, 2259
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 2 cut(s) 2207, 2259
Eco130I CCWWGG 5 cut(s) 515, 1051, 1148, 1620, 2968
Eco24I GRGCYC 3 cut(s) 769, 1051, 2092
Eco32I GATATC 4 cut(s) 713, 998, 1701, 2182
Eco47I GGWCC 5 cut(s) 257, 575, 665, 824, 2624
Eco57I CTGAAG 4 cut(s) 372, 1449, 1605, 2669
Eco91I GGTNACC 1 cut(s) 2129
EcoO109I RGGNCCY 4 cut(s) 659, 1047, 1048, 2624
EcoO65I GGTNACC 1 cut(s) 2129
EcoRI GAATTC 2 cut(s) 1397, 2663
EcoRII CCWGG 8 cut(s) 259, 335, 661, 667, 973, 1527, 2626, 2739
EcoRV GATATC 4 cut(s) 713, 998, 1701, 2182
EcoT14I CCWWGG 5 cut(s) 515, 1051, 1148, 1620, 2968
EcoT22I ATGCAT 3 cut(s) 839, 2360, 2469
EcoT38I GRGCYC 3 cut(s) 769, 1051, 2092
ErhI CCWWGG 5 cut(s) 515, 1051, 1148, 1620, 2968
Esp3I CGTCTC 1 cut(s) 81
FaqI GGGAC 3 cut(s) 406, 1888, 2949
FauI CCCGC 2 cut(s) 187, 1854
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 1837
FblI GTMKAC 2 cut(s) 1264, 1496
FokI GGATG 6 cut(s) 266, 908, 959, 2732, 2838, 2929
FriOI GRGCYC 3 cut(s) 769, 1051, 2092
FspBI CTAG 6 cut(s) 483, 1143, 2061, 2619, 2810, 2955
GlaI GCGC 2 cut(s) 120, 2141
GsaI CCCAGC 2 cut(s) 1460, 1731
GsuI CTGGAG 7 cut(s) 1062, 1511, 1773, 1863, 1971, 2196, 2501
HgaI GACGC 2 cut(s) 2062, 2510
HhaI GCGC 2 cut(s) 121, 2142
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 2 cut(s) 119, 2140
HinP1I GCGC 2 cut(s) 119, 2140
HincII GTYRAC 3 cut(s) 285, 1425, 2221
HindII GTYRAC 3 cut(s) 285, 1425, 2221
HinfI GANTC 7 cut(s) 11, 184, 394, 1076, 1721, 1876, 2512
Hpy188I TCNGA 7 cut(s) 156, 865, 1675, 2349, 2575, 2751, 2841
Hpy188III TCNNGA 9 cut(s) 41, 368, 611, 770, 993, 1041, 1439, 1880, 2098
Hpy99I CGWCG 3 cut(s) 22, 73, 76
HpyAV CCTTC 7 cut(s) 86, 996, 1383, 1847, 2175, 2263, 2594
HpyCH4III ACNGT 6 cut(s) 65, 1166, 1422, 2042, 2411, 2594
HpyCH4IV ACGT 8 cut(s) 6, 71, 215, 281, 447, 462, 1027, 1267
HpyF3I CTNAG 8 cut(s) 78, 153, 594, 1590, 2008, 2046, 2084, 2206
HpySE526I ACGT 8 cut(s) 6, 71, 215, 281, 447, 462, 1027, 1267
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 2 cut(s) 119, 2140
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 1837
KspI CCGCGG 1 cut(s) 203
LweI GCATC 8 cut(s) 886, 1162, 1177, 1583, 1774, 2407, 2755, 2907
MaeI CTAG 6 cut(s) 483, 1143, 2061, 2619, 2810, 2955
MaeII ACGT 8 cut(s) 6, 71, 215, 281, 447, 462, 1027, 1267
MaeIII GTNAC 6 cut(s) 1138, 1238, 1487, 2036, 2129, 2635
MfeI CAATTG 3 cut(s) 723, 966, 1833
MflI RGATCY 4 cut(s) 134, 598, 2029, 2824
MhlI GDGCHC 5 cut(s) 317, 769, 1051, 2092, 2704
MlsI TGGCCA 1 cut(s) 276
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 4 cut(s) 5, 178, 388, 1870
MmeI TCCRAC 4 cut(s) 236, 786, 1077, 2969
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 3 cut(s) 839, 2360, 2469
MroNI GCCGGC 1 cut(s) 416
MscI TGGCCA 1 cut(s) 276
MseI TTAA 6 cut(s) 1020, 1382, 1635, 1964, 2297, 2418
MslI CAYNNNNRTG 6 cut(s) 456, 832, 945, 2462, 2707, 2709
Msp20I TGGCCA 1 cut(s) 276
MunI CAATTG 3 cut(s) 723, 966, 1833
Mva1269I GAATGC 2 cut(s) 2360, 2935
MvaI CCWGG 8 cut(s) 261, 337, 663, 669, 975, 1529, 2628, 2741
MvnI CGCG 1 cut(s) 202
NaeI GCCGGC 1 cut(s) 418
NciI CCSGG 5 cut(s) 126, 321, 1537, 2225, 2800
NdeI CATATG 1 cut(s) 925
NgoMIV GCCGGC 1 cut(s) 416
NmuCI GTSAC 4 cut(s) 1238, 1487, 2036, 2129
NsiI ATGCAT 3 cut(s) 839, 2360, 2469
NspI RCATGY 8 cut(s) 530, 689, 841, 1007, 1037, 1223, 1346, 1495
PaeI GCATGC 1 cut(s) 1346
PaqCI CACCTGC 1 cut(s) 422
PasI CCCWGGG 1 cut(s) 2740
PciI ACATGT 3 cut(s) 526, 685, 1491
PcsI WCGNNNNNNNCGW 2 cut(s) 2190, 2407
PctI GAATGC 2 cut(s) 2360, 2935
PdiI GCCGGC 1 cut(s) 418
PfeI GAWTC 3 cut(s) 1076, 1721, 2512
PflFI GACNNNGTC 1 cut(s) 73
PflMI CCANNNNNTGG 4 cut(s) 272, 680, 1484, 1751
PleI GAGTC 4 cut(s) 5, 178, 388, 1870
PpsI GAGTC 4 cut(s) 5, 178, 388, 1870
Ppu21I YACGTR 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 2624
PscI ACATGT 3 cut(s) 526, 685, 1491
PshAI GACNNNNGTC 1 cut(s) 1921
Psp5II RGGWCCY 1 cut(s) 2624
Psp6I CCWGG 8 cut(s) 259, 335, 661, 667, 973, 1527, 2626, 2739
PspEI GGTNACC 1 cut(s) 2129
PspFI CCCAGC 2 cut(s) 1456, 1727
PspGI CCWGG 8 cut(s) 259, 335, 661, 667, 973, 1527, 2626, 2739
PspOMI GGGCCC 1 cut(s) 1047
PspPPI RGGWCCY 1 cut(s) 2624
PstI CTGCAG 8 cut(s) 1108, 1195, 1231, 1351, 1357, 1792, 1897, 2323
PstNI CAGNNNCTG 3 cut(s) 1439, 1583, 1796
PsuI RGATCY 4 cut(s) 134, 598, 2029, 2824
PsyI GACNNNGTC 1 cut(s) 73
PvuII CAGCTG 8 cut(s) 407, 539, 779, 1226, 1352, 1358, 1583, 1751
RsaI GTAC 6 cut(s) 67, 218, 1371, 1510, 2292, 2785
RsaNI GTAC 6 cut(s) 66, 217, 1370, 1509, 2291, 2784
RseI CAYNNNNRTG 6 cut(s) 456, 832, 945, 2462, 2707, 2709
SacII CCGCGG 1 cut(s) 203
SaqAI TTAA 6 cut(s) 1020, 1382, 1635, 1964, 2297, 2418
ScaI AGTACT 1 cut(s) 1510
SchI GAGTC 4 cut(s) 5, 178, 388, 1870
SduI GDGCHC 5 cut(s) 317, 769, 1051, 2092, 2704
SfaNI GCATC 8 cut(s) 886, 1162, 1177, 1583, 1774, 2407, 2755, 2907
SfcI CTRYAG 9 cut(s) 540, 1104, 1191, 1227, 1347, 1353, 1788, 1893, 2319
Sfr303I CCGCGG 1 cut(s) 203
SgrBI CCGCGG 1 cut(s) 203
SinI GGWCC 5 cut(s) 257, 575, 665, 824, 2624
SmiMI CAYNNNNRTG 6 cut(s) 456, 832, 945, 2462, 2707, 2709
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
SpeI ACTAGT 1 cut(s) 1142
SphI GCATGC 1 cut(s) 1346
SspI AATATT 2 cut(s) 1741, 2055
SspMI CTAG 6 cut(s) 483, 1143, 2061, 2619, 2810, 2955
StyI CCWWGG 5 cut(s) 515, 1051, 1148, 1620, 2968
TaaI ACNGT 6 cut(s) 65, 1166, 1422, 2042, 2411, 2594
TaiI ACGT 8 cut(s) 9, 74, 218, 284, 450, 465, 1030, 1270
TaqI TCGA 8 cut(s) 20, 111, 634, 771, 1709, 1915, 2193, 2401
TatI WGTACW 3 cut(s) 1369, 1508, 2290
TauI GCSGC 8 cut(s) 86, 164, 167, 202, 205, 299, 386, 1065
TfiI GAWTC 3 cut(s) 1076, 1721, 2512
Tru1I TTAA 6 cut(s) 1020, 1382, 1635, 1964, 2297, 2418
Tru9I TTAA 6 cut(s) 1020, 1382, 1635, 1964, 2297, 2418
TscAI CASTG 8 cut(s) 57, 1098, 1171, 1606, 1693, 2047, 2436, 2599
TseFI GTSAC 4 cut(s) 1238, 1487, 2036, 2129
Tsp45I GTSAC 4 cut(s) 1238, 1487, 2036, 2129
TspGWI ACGGA 1 cut(s) 2291
TspRI CASTG 8 cut(s) 57, 1098, 1171, 1606, 1693, 2047, 2436, 2599
Tth111I GACNNNGTC 1 cut(s) 73
Van91I CCANNNNNTGG 4 cut(s) 272, 680, 1484, 1751
VneI GTGCAC 1 cut(s) 2700
VpaK11BI GGWCC 5 cut(s) 257, 575, 665, 824, 2624
XapI RAATTY 3 cut(s) 1397, 2385, 2663
XceI RCATGY 8 cut(s) 530, 689, 841, 1007, 1037, 1223, 1346, 1495
XcmI CCANNNNNNNNNTGG 2 cut(s) 1948, 2554
XmiI GTMKAC 2 cut(s) 1264, 1496
XspI CTAG 6 cut(s) 483, 1143, 2061, 2619, 2810, 2955
ZraI GACGTC 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 1510
Zsp2I ATGCAT 3 cut(s) 839, 2360, 2469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.