Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0002717

Physical Location & Seq

Genomic Coordinates Reverse (-)

41719212 .. 41731799

Gene Structure 12588 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG355900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGACCAGGTATGCAACTGCAAGTGCAAGGTCGGATGGTCTTCTCCTCCTTTGTGGAGGGAGGGATGCAAACAGTGTGCCATTGGCAAGTGCATATGGCCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCCCCTTCGCCCAGATATCAACATGCAGCAGTTTTTGTTAATGCACGCTTGCATGTTTCTGGAGGGGCCCTTGGTGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCTGGGGTTTGGTGTGATACAAAATCTGTTGTCACGAGTCCAAGGACAGGTAGATACAGCGCTGATGCAGCTGGTGGAGATGCTGCAGTTGAATTGACACGGCGTTGCAGGCACGCAGCTGCTGCGGTTGGTGACCTGATATTTATATACGGTGGTCTACGTGGTGGGGTGCTGCTTGATGACCTACTGGTTGCGGAAGATCTGGCTGCTGCAGAAACAACAACTGCAGCCTCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGCCGTTTGCCTGGAAGATATGGATTCATTGATGAAAGAACGAGGCAGATAATGCCTGAAGCAGCACCTGATGGTGCTGTTGTGCTGGGAAGTCCAGTTGCCCCACCTGTAAATGGAGACATGTATACTGATATAAGCACCGAAAATGCCTTGCTCCAGGGATCCAGGAGACTGAGCAAAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGTGCTACTTTGGCTGCGGCCAAGGCACGACAAGTTAATGGAGAAGTAGAGTTACCTGATAGAGATCGTGGCTCAGAGGCTACCCCGAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCGTCAGCTGGAGTGCGACTGCATCATAGAGCTGTTGTTGTAGCTGCAGAGACTGGGGGAGCTTTGGGCGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCAGAGAGTGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCCAAAAAGGTAATAGCACATCTTTTAAAGCCACGTGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTCTGTGATAGTGCTGAACGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATATTTGGTGATTTACACGGGCAATTTGGCGATCTCATGCGCCTTTTTGATGAGTATGGTTCACCTTCAACTGCTGGGGATATCGCATATATCGACTATCTCTTCCTAGGAGACTATGTTGACCGAGGCCAACATAGCTTGGAAACCATTTCTCTTCTGCTTGCTTTGAAGGTTGAATATTCCCAAAATGTACACTTAATTAGGGGTAATCATGAAGCTGCAGATATTAATGCTCTTTTTGGCTTTCGGATCGAATGCATTGAGCGAATGGGTGAGAGAGACGGAATATGGGCATGGCATCGAATAAACCGTTTGTTCAATTGGCTTCCTCTAGCTGCTCTAATTGAGAAGAAAATCATTTGTATGCATGGTGGTATTGGTCGGTCAATAAATCATGTTGAACAGATTGAGAATCTCCAGCGTCCTATTACAATGGAAGCAGGCTCGATCGTTCTTATGGATTTATTATGGTCTGACCCAACAGAAAATGATAGTGTGGAAGGATTGCGACCAAATGCTAGGGGTCCTGGATTGGTTACTTTTGGGCCTGATCGTGTCATGGAGTTCTGCAACAACAATGATCTTCAGTTGATTGTACGCGCTCATGAATGTGTAATGGATGGCTTTGAACGATTTGCCCAGGGACATTTGATTACACTTTTCTCTGCTACCAATTACTGTGGTACAGCAAATAATGCAGGGGCAATCCTTGTTTTAGGTAGAGATCTTGTGGTGGTCCCGAAACTCATTCATCCCTTACCACCGGCAATTTCTTCACCAGAAACATCGCCCGAACGACATATTGAAGATACGTGGATGCAGGAACTGAATGCTAACAGACCTCCAACACCAACTAGAGGCCGTCCACAAGTAGCAAATGACCGGGGATCCCTTGCTTGGATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0016020 GO:0044424 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_2 Kelch_4 Metallophos

Protein Analysis

727

Amino Acids

78.41

Weight (kDa)

5.92

Isoelectric Point (pI)

41.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_KLHDC2_KLHL20_DRC7	PF24681	12 - 164	4.9e-09	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2	PF07646	54 - 101	4e-07	Kelch motif
Metallophos	PF00149	424 - 631	4.6e-39	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002717)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08420 AT1G08420 AT2G27210 AT2G27210
fragaria_vesca	FvH4_3g26300
malus_domestica	MD03G1156600.v1.1 MD03G1157300.v1.1 MD11G1174100.v1.1
prunus_persica	Prupe.6G145800_v2.0.a1 Prupe.6G145800_v2.0.a1 Prupe.6G145800_v2.0.a1 Prupe.6G145800_v2.0.a1 Prupe.6G145800_v2.0.a1
pyrus_communis	pycom03g11040 pycom11g14860
rosa_chinensis	RchiOBHm_Chr5g0048611 RchiOBHm_Chr7g0219991 RchiOBHm_Chr7g0220001
rosa_laevigata	RLG00000034543
rosa_multiflora	Rmu_co8183230.1_g000001 Rmu_co8284673.1_g000001 Rmu_sc0005838.1_g000012 Rmu_sc0005838.1_g000013 Rmu_sc0007553.1_g000001 Rmu_sc0008151.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033170 Rroxscaffold_3G00239830
rosa_rugosa	Rorug05G0240500 Rorug07G0188300
rosa_samantha	Rh5AG319700 Rh5CG355900 Rh7AG332100 Rh7CG349500 Rh7CG349600 Rh7DG327700 Rh7DG327800
rosa_wichuraiana	Rw0G021290 Rw5G029900 Rw7G027970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	867
AccI	GTMKAC	2 cut(s)	433, 674
AccII	CGCG	1 cut(s)	1878
AciI	CCGC	6 cut(s)	401, 470, 794, 867, 990, 1039
AclWI	GGATC	5 cut(s)	705, 718, 1535, 2160, 2173
AcoI	YGGCCR	2 cut(s)	118, 795
AcuI	CTGAAG	2 cut(s)	627, 1847
AcvI	CACGTG	1 cut(s)	1151
AfaI	GTAC	3 cut(s)	1470, 1875, 1963
AfeI	AGCGCT	1 cut(s)	337
AfiI	CCNNNNNNNGG	7 cut(s)	111, 323, 662, 867, 929, 2135, 2175
AflIII	ACRYGT	1 cut(s)	669
AjnI	CCWGG	8 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917
Alw26I	GTCTC	6 cut(s)	154, 660, 712, 965, 1383, 1551
AlwI	GGATC	5 cut(s)	705, 718, 1535, 2160, 2173
AlwNI	CAGNNNCTG	5 cut(s)	398, 617, 767, 974, 2104
Ama87I	CYCGRG	1 cut(s)	862
Aor51HI	AGCGCT	1 cut(s)	337
AoxI	GGCC	8 cut(s)	97, 118, 216, 550, 795, 1405, 1823, 2137
ApaI	GGGCCC	1 cut(s)	220
AseI	ATTAAT	2 cut(s)	1107, 1506
AspA2I	CCTAGG	1 cut(s)	1384
AspLEI	GCGC	4 cut(s)	234, 338, 1320, 1880
AspS9I	GGNCC	6 cut(s)	216, 217, 1053, 1802, 1823, 2014
AsuC2I	CCSGG	1 cut(s)	2162
AsuHPI	GGTGA	6 cut(s)	419, 1009, 1298, 1332, 1562, 2046
AvaI	CYCGRG	1 cut(s)	862
AvaII	GGWCC	3 cut(s)	1053, 1802, 2014
AvrII	CCTAGG	1 cut(s)	1384
BaeGI	GKGCMC	2 cut(s)	220, 1119
BamHI	GGATCC	2 cut(s)	710, 2165
BanII	GRGCYC	2 cut(s)	220, 1270
BarI	GAAGNNNNNNTAC	2 cut(s)	813, 845
BauI	CACGAG	1 cut(s)	310
BbrPI	CACGTG	1 cut(s)	1151
BbsI	GAAGAC	1 cut(s)	32
BbvCI	CCTCAGC	1 cut(s)	768
BccI	CCATC	5 cut(s)	29, 110, 614, 1149, 1892
BceAI	ACGGC	4 cut(s)	105, 392, 537, 2124
BciT130I	CCWGG	8 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919
BciVI	GTATCC	1 cut(s)	262
BclI	TGATCA	1 cut(s)	888
BcnI	CCSGG	1 cut(s)	2162
BcoDI	GTCTC	6 cut(s)	154, 660, 712, 965, 1383, 1551
BfaI	CTAG	5 cut(s)	1239, 1385, 1610, 1797, 2133
BfmI	CTRYAG	9 cut(s)	273, 360, 486, 501, 516, 522, 966, 1071, 1497
BfoI	RGCGCY	1 cut(s)	339
BfuI	GTATCC	1 cut(s)	262
BglII	AGATCT	3 cut(s)	475, 1207, 2002
BlnI	CCTAGG	1 cut(s)	1384
Bme1390I	CCNGG	9 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919, 2162
Bme18I	GGWCC	3 cut(s)	1053, 1802, 2014
BmeT110I	CYCGRG	1 cut(s)	862
BmgT120I	GGNCC	6 cut(s)	216, 217, 1053, 1802, 1823, 2014
BmiI	GGNNCC	9 cut(s)	217, 218, 712, 1054, 1055, 1269, 1803, 2016, 2167
BmrFI	CCNGG	9 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919, 2162
BmrI	ACTGGG	1 cut(s)	984
BmsI	GCATC	7 cut(s)	55, 331, 346, 761, 952, 1585, 2085
BmuI	ACTGGG	1 cut(s)	984
BpiI	GAAGAC	1 cut(s)	32
BpmI	CTGGAG	4 cut(s)	231, 689, 951, 1679
Bpu10I	CCTNAGC	1 cut(s)	768
BpuMI	CCSGG	1 cut(s)	2162
BsaAI	YACGTR	3 cut(s)	437, 1151, 2091
BsaBI	GATNNNNATC	1 cut(s)	840
BsaJI	CCNNGG	9 cut(s)	220, 317, 706, 798, 1384, 1402, 1917, 1918, 2161
BsaXI	ACNNNNNCTCC	2 cut(s)	1380, 1410
Bsc4I	CCNNNNNNNGG	7 cut(s)	111, 323, 662, 867, 929, 2135, 2175
Bse118I	RCCGGY	1 cut(s)	2041
Bse1I	ACTGG	3 cut(s)	468, 644, 979
Bse3DI	GCAATG	1 cut(s)	1201
Bse8I	GATNNNNATC	1 cut(s)	840
BseBI	CCWGG	8 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919
BseDI	CCNNGG	9 cut(s)	220, 317, 706, 798, 1384, 1402, 1917, 1918, 2161
BseGI	GGATG	7 cut(s)	40, 70, 121, 1160, 1903, 2029, 2100
BseJI	GATNNNNATC	1 cut(s)	840
BseLI	CCNNNNNNNGG	7 cut(s)	111, 323, 662, 867, 929, 2135, 2175
BseMI	GCAATG	1 cut(s)	1201
BseMII	CTCAG	4 cut(s)	545, 713, 759, 864
BseNI	ACTGG	3 cut(s)	468, 644, 979
BseRI	GAGGAG	1 cut(s)	35
BseSI	GKGCMC	2 cut(s)	220, 1119
BseYI	CCCAGC	4 cut(s)	278, 634, 905, 1352
Bsh1236I	CGCG	1 cut(s)	1878
Bsh1285I	CGRYCG	1 cut(s)	1728
BshFI	GGCC	8 cut(s)	99, 120, 218, 552, 797, 1407, 1825, 2139
BsiEI	CGRYCG	1 cut(s)	1728
BsiHKCI	CYCGRG	1 cut(s)	862
BsiSI	CCGG	2 cut(s)	2042, 2161
BslFI	GGGAC	3 cut(s)	1066, 1935, 2000
BslI	CCNNNNNNNGG	7 cut(s)	111, 323, 662, 867, 929, 2135, 2175
BsmAI	GTCTC	6 cut(s)	154, 660, 712, 965, 1383, 1551
BsmBI	CGTCTC	1 cut(s)	1551
BsmFI	GGGAC	3 cut(s)	1066, 1935, 2000
BsmI	GAATGC	2 cut(s)	1538, 2113
BsnI	GGCC	8 cut(s)	99, 120, 218, 552, 797, 1407, 1825, 2139
BsoBI	CYCGRG	1 cut(s)	862
Bsp120I	GGGCCC	1 cut(s)	216
Bsp1286I	GDGCHC	3 cut(s)	220, 1119, 1270
Bsp1407I	TGTACA	1 cut(s)	1468
BspACI	CCGC	6 cut(s)	401, 470, 794, 867, 990, 1039
BspANI	GGCC	8 cut(s)	99, 120, 218, 552, 797, 1407, 1825, 2139
BspCNI	CTCAG	4 cut(s)	544, 714, 760, 863
BspFNI	CGCG	1 cut(s)	1878
BspHI	TCATGA	2 cut(s)	1489, 1882
BspLI	GGNNCC	9 cut(s)	217, 218, 712, 1054, 1055, 1269, 1803, 2016, 2167
BspMAI	CTGCAG	9 cut(s)	277, 364, 490, 505, 520, 526, 970, 1075, 1501
BspPI	GGATC	5 cut(s)	705, 718, 1535, 2160, 2173
BsrBI	CCGCTC	1 cut(s)	867
BsrDI	GCAATG	1 cut(s)	1201
BsrFI	RCCGGY	1 cut(s)	2041
BsrGI	TGTACA	1 cut(s)	1468
BsrI	ACTGG	3 cut(s)	468, 644, 979
BssAI	RCCGGY	1 cut(s)	2041
BssECI	CCNNGG	9 cut(s)	220, 317, 706, 798, 1384, 1402, 1917, 1918, 2161
BssNAI	GTATAC	1 cut(s)	675
BssSI	CACGAG	1 cut(s)	310
BssT1I	CCWWGG	4 cut(s)	220, 317, 798, 1384
Bst1107I	GTATAC	1 cut(s)	675
Bst2BI	CACGAG	1 cut(s)	310
Bst2UI	CCWGG	8 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919
Bst4CI	ACNGT	4 cut(s)	74, 428, 1589, 1958
Bst6I	CTCTTC	2 cut(s)	1385, 1437
BstAPI	GCANNNNNTGC	4 cut(s)	398, 601, 779, 1973
BstAUI	TGTACA	1 cut(s)	1468
BstBAI	YACGTR	3 cut(s)	437, 1151, 2091
BstC8I	GCNNGC	9 cut(s)	196, 200, 386, 390, 513, 1037, 1175, 1440, 1720
BstDEI	CTNAG	5 cut(s)	531, 722, 768, 850, 1262
BstEII	GGTNACC	1 cut(s)	407
BstF5I	GGATG	7 cut(s)	40, 70, 121, 1160, 1903, 2029, 2100
BstFNI	CGCG	1 cut(s)	1878
BstH2I	RGCGCY	1 cut(s)	339
BstHHI	GCGC	4 cut(s)	234, 338, 1320, 1880
BstMAI	GTCTC	6 cut(s)	154, 660, 712, 965, 1383, 1551
BstMCI	CGRYCG	1 cut(s)	1728
BstNI	CCWGG	8 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919
BstNSI	RCATGY	4 cut(s)	176, 206, 515, 673
BstPI	GGTNACC	1 cut(s)	407
BstSCI	CCNGG	9 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917, 2160
BstSFI	CTRYAG	9 cut(s)	273, 360, 486, 501, 516, 522, 966, 1071, 1497
BstSLI	GKGCMC	2 cut(s)	220, 1119
BstUI	CGCG	1 cut(s)	1878
BstV2I	GAAGAC	1 cut(s)	32
BstX2I	RGATCY	5 cut(s)	475, 710, 1207, 2002, 2165
BstXI	CCANNNNNNTGG	1 cut(s)	1155
BstYI	RGATCY	5 cut(s)	475, 710, 1207, 2002, 2165
BstZ17I	GTATAC	1 cut(s)	675
BsuI	GTATCC	1 cut(s)	262
BsuRI	GGCC	8 cut(s)	99, 120, 218, 552, 797, 1407, 1825, 2139
BtgZI	GCGATG	1 cut(s)	2049
BtsCI	GGATG	7 cut(s)	40, 70, 121, 1160, 1903, 2029, 2100
BtsI	GCAGTG	1 cut(s)	267
BtsIMutI	CAGTG	3 cut(s)	79, 267, 784
Cac8I	GCNNGC	9 cut(s)	196, 200, 386, 390, 513, 1037, 1175, 1440, 1720
CaiI	CAGNNNCTG	5 cut(s)	398, 617, 767, 974, 2104
CciI	TCATGA	2 cut(s)	1489, 1882
CfoI	GCGC	4 cut(s)	234, 338, 1320, 1880
Cfr10I	RCCGGY	1 cut(s)	2041
Cfr13I	GGNCC	6 cut(s)	216, 217, 1053, 1802, 1823, 2014
CseI	GACGC	1 cut(s)	1688
CsiI	ACCWGGT	1 cut(s)	5
Csp6I	GTAC	3 cut(s)	1469, 1874, 1962
CspCI	CAANNNNNGTGG	2 cut(s)	1939, 1974
CviQI	GTAC	3 cut(s)	1469, 1874, 1962
DdeI	CTNAG	5 cut(s)	531, 722, 768, 850, 1262
DraI	TTTAAA	1 cut(s)	1143
EaeI	YGGCCR	2 cut(s)	118, 795
Eam1104I	CTCTTC	2 cut(s)	1385, 1437
EarI	CTCTTC	2 cut(s)	1385, 1437
Eco130I	CCWWGG	4 cut(s)	220, 317, 798, 1384
Eco24I	GRGCYC	2 cut(s)	220, 1270
Eco32I	GATATC	2 cut(s)	167, 1360
Eco47I	GGWCC	3 cut(s)	1053, 1802, 2014
Eco47III	AGCGCT	1 cut(s)	337
Eco57I	CTGAAG	2 cut(s)	627, 1847
Eco72I	CACGTG	1 cut(s)	1151
Eco88I	CYCGRG	1 cut(s)	862
Eco91I	GGTNACC	1 cut(s)	407
EcoO109I	RGGNCCY	4 cut(s)	216, 217, 1053, 1802
EcoO65I	GGTNACC	1 cut(s)	407
EcoRII	CCWGG	8 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917
EcoRV	GATATC	2 cut(s)	167, 1360
EcoT14I	CCWWGG	4 cut(s)	220, 317, 798, 1384
EcoT22I	ATGCAT	2 cut(s)	1538, 1647
EcoT38I	GRGCYC	2 cut(s)	220, 1270
ErhI	CCWWGG	4 cut(s)	220, 317, 798, 1384
Esp3I	CGTCTC	1 cut(s)	1551
FaqI	GGGAC	3 cut(s)	1066, 1935, 2000
FauI	CCCGC	2 cut(s)	860, 1032
FauNDI	CATATG	1 cut(s)	94
FbaI	TGATCA	1 cut(s)	888
FblI	GTMKAC	2 cut(s)	433, 674
FokI	GGATG	7 cut(s)	47, 77, 128, 1167, 1910, 2016, 2107
FriOI	GRGCYC	2 cut(s)	220, 1270
FspBI	CTAG	5 cut(s)	1239, 1385, 1610, 1797, 2133
GlaI	GCGC	4 cut(s)	233, 337, 1319, 1879
GsaI	CCCAGC	4 cut(s)	282, 638, 909, 1356
GsuI	CTGGAG	4 cut(s)	231, 689, 951, 1679
HaeII	RGCGCY	1 cut(s)	339
HaeIII	GGCC	8 cut(s)	99, 120, 218, 552, 797, 1407, 1825, 2139
HapII	CCGG	2 cut(s)	2042, 2161
HgaI	GACGC	1 cut(s)	1688
HhaI	GCGC	4 cut(s)	234, 338, 1320, 1880
Hin6I	GCGC	4 cut(s)	232, 336, 1318, 1878
HinP1I	GCGC	4 cut(s)	232, 336, 1318, 1878
HincII	GTYRAC	1 cut(s)	1399
HindII	GTYRAC	1 cut(s)	1399
HinfI	GANTC	4 cut(s)	245, 313, 573, 1690
HpaII	CCGG	2 cut(s)	2042, 2161
HphI	GGTGA	6 cut(s)	419, 1009, 1298, 1332, 1562, 2046
Hpy166II	GTNNAC	7 cut(s)	434, 675, 1244, 1340, 1399, 1471, 2144
Hpy188I	TCNGA	4 cut(s)	34, 853, 1527, 1753
Hpy188III	TCNNGA	6 cut(s)	210, 310, 1276, 1490, 1883, 2017
Hpy8I	GTNNAC	7 cut(s)	434, 675, 1244, 1340, 1399, 1471, 2144
HpyAV	CCTTC	5 cut(s)	165, 1025, 1353, 1441, 1772
HpyCH4III	ACNGT	4 cut(s)	74, 428, 1589, 1958
HpyCH4IV	ACGT	3 cut(s)	436, 1150, 2090
HpyF3I	CTNAG	5 cut(s)	531, 722, 768, 850, 1262
HpySE526I	ACGT	3 cut(s)	436, 1150, 2090
HspAI	GCGC	4 cut(s)	232, 336, 1318, 1878
KflI	GGGWCCC	1 cut(s)	1053
Ksp22I	TGATCA	1 cut(s)	888
LmnI	GCTCC	4 cut(s)	145, 708, 980, 1273
LweI	GCATC	7 cut(s)	55, 331, 346, 761, 952, 1585, 2085
MabI	ACCWGGT	1 cut(s)	5
MaeI	CTAG	5 cut(s)	1239, 1385, 1610, 1797, 2133
MaeII	ACGT	3 cut(s)	436, 1150, 2090
MaeIII	GTNAC	4 cut(s)	307, 407, 828, 1813
MbiI	CCGCTC	1 cut(s)	867
MfeI	CAATTG	2 cut(s)	135, 1597
MflI	RGATCY	5 cut(s)	475, 710, 1207, 2002, 2165
MhlI	GDGCHC	3 cut(s)	220, 1119, 1270
MlyI	GAGTC	1 cut(s)	322
MmeI	TCCRAC	3 cut(s)	12, 246, 2147
Mph1103I	ATGCAT	2 cut(s)	1538, 1647
MseI	TTAA	6 cut(s)	189, 813, 1107, 1142, 1475, 1506
MslI	CAYNNNNRTG	4 cut(s)	114, 1153, 1640, 1887
MspA1I	CMGCKG	6 cut(s)	278, 347, 395, 521, 527, 929
MspI	CCGG	2 cut(s)	2042, 2161
MspR9I	CCNGG	9 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919, 2162
MunI	CAATTG	2 cut(s)	135, 1597
Mva1269I	GAATGC	2 cut(s)	1538, 2113
MvaI	CCWGG	8 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919
MvnI	CGCG	1 cut(s)	1878
NciI	CCSGG	1 cut(s)	2162
NdeI	CATATG	1 cut(s)	94
NlaIV	GGNNCC	9 cut(s)	217, 218, 712, 1054, 1055, 1269, 1803, 2016, 2167
NmuCI	GTSAC	2 cut(s)	307, 407
NsiI	ATGCAT	2 cut(s)	1538, 1647
NspI	RCATGY	4 cut(s)	176, 206, 515, 673
PaeI	GCATGC	1 cut(s)	515
PagI	TCATGA	2 cut(s)	1489, 1882
PasI	CCCWGGG	1 cut(s)	1918
PciI	ACATGT	1 cut(s)	669
PcsI	WCGNNNNNNNCGW	2 cut(s)	1368, 1585
PctI	GAATGC	2 cut(s)	1538, 2113
PfeI	GAWTC	3 cut(s)	245, 573, 1690
PfoI	TCCNGGA	2 cut(s)	713, 1804
Ple19I	CGATCG	1 cut(s)	1728
PleI	GAGTC	1 cut(s)	321
PmaCI	CACGTG	1 cut(s)	1151
PmlI	CACGTG	1 cut(s)	1151
PpsI	GAGTC	1 cut(s)	321
Ppu21I	YACGTR	3 cut(s)	437, 1151, 2091
PpuMI	RGGWCCY	2 cut(s)	1053, 1802
PscI	ACATGT	1 cut(s)	669
PshBI	ATTAAT	2 cut(s)	1107, 1506
Psp5II	RGGWCCY	2 cut(s)	1053, 1802
Psp6I	CCWGG	8 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917
PspCI	CACGTG	1 cut(s)	1151
PspEI	GGTNACC	1 cut(s)	407
PspFI	CCCAGC	4 cut(s)	278, 634, 905, 1352
PspGI	CCWGG	8 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917
PspN4I	GGNNCC	9 cut(s)	217, 218, 712, 1054, 1055, 1269, 1803, 2016, 2167
PspOMI	GGGCCC	1 cut(s)	216
PspPI	GGNCC	6 cut(s)	216, 217, 1053, 1802, 1823, 2014
PspPPI	RGGWCCY	2 cut(s)	1053, 1802
PstI	CTGCAG	9 cut(s)	277, 364, 490, 505, 520, 526, 970, 1075, 1501
PstNI	CAGNNNCTG	5 cut(s)	398, 617, 767, 974, 2104
PsuI	RGATCY	5 cut(s)	475, 710, 1207, 2002, 2165
PvuI	CGATCG	1 cut(s)	1728
PvuII	CAGCTG	6 cut(s)	278, 347, 395, 521, 527, 929
RsaI	GTAC	3 cut(s)	1470, 1875, 1963
RsaNI	GTAC	3 cut(s)	1469, 1874, 1962
RseI	CAYNNNNRTG	4 cut(s)	114, 1153, 1640, 1887
SaqAI	TTAA	6 cut(s)	189, 813, 1107, 1142, 1475, 1506
Sau96I	GGNCC	6 cut(s)	216, 217, 1053, 1802, 1823, 2014
SchI	GAGTC	1 cut(s)	322
ScrFI	CCNGG	9 cut(s)	7, 144, 549, 561, 707, 715, 1806, 1919, 2162
SduI	GDGCHC	3 cut(s)	220, 1119, 1270
SexAI	ACCWGGT	1 cut(s)	5
SfaNI	GCATC	7 cut(s)	55, 331, 346, 761, 952, 1585, 2085
SfcI	CTRYAG	9 cut(s)	273, 360, 486, 501, 516, 522, 966, 1071, 1497
SinI	GGWCC	3 cut(s)	1053, 1802, 2014
SmiMI	CAYNNNNRTG	4 cut(s)	114, 1153, 1640, 1887
SphI	GCATGC	1 cut(s)	515
SsiI	CCGC	6 cut(s)	401, 470, 794, 867, 990, 1039
SspI	AATATT	2 cut(s)	919, 1457
SspMI	CTAG	5 cut(s)	1239, 1385, 1610, 1797, 2133
StyD4I	CCNGG	9 cut(s)	5, 142, 547, 559, 705, 713, 1804, 1917, 2160
StyI	CCWWGG	4 cut(s)	220, 317, 798, 1384
TaaI	ACNGT	4 cut(s)	74, 428, 1589, 1958
TaiI	ACGT	3 cut(s)	439, 1153, 2093
TaqI	TCGA	4 cut(s)	1371, 1530, 1579, 1724
TaqII	GACCGA	2 cut(s)	1416, 1650
TatI	WGTACW	1 cut(s)	1468
TauI	GCSGC	2 cut(s)	797, 993
TfiI	GAWTC	3 cut(s)	245, 573, 1690
Tru1I	TTAA	6 cut(s)	189, 813, 1107, 1142, 1475, 1506
Tru9I	TTAA	6 cut(s)	189, 813, 1107, 1142, 1475, 1506
TscAI	CASTG	3 cut(s)	79, 267, 784
TseFI	GTSAC	2 cut(s)	307, 407
Tsp45I	GTSAC	2 cut(s)	307, 407
TspDTI	ATGAA	8 cut(s)	237, 565, 597, 1044, 1212, 1506, 1899, 2018
TspGWI	ACGGA	3 cut(s)	912, 1243, 1575
TspRI	CASTG	3 cut(s)	79, 267, 784
VpaK11BI	GGWCC	3 cut(s)	1053, 1802, 2014
VspI	ATTAAT	2 cut(s)	1107, 1506
XceI	RCATGY	4 cut(s)	176, 206, 515, 673
XmaJI	CCTAGG	1 cut(s)	1384
XmiI	GTMKAC	2 cut(s)	433, 674
XspI	CTAG	5 cut(s)	1239, 1385, 1610, 1797, 2133
Zsp2I	ATGCAT	2 cut(s)	1538, 1647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.