AT2G28490

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001754
Physical Location & Seq
Reverse (-)
12178597 .. 12181012
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28490.1

Sequence Viewer

Length: 1536 bp
ATGGAGAAAAACAAGAGAGCTATCGGATTTTTACTTCTGGTCGTACTTATCAATGGAGTTATGATGACGAGATCAAATGGGTATGAAGGAGAAGAAGAGTGGGGAGGAGCAGGAGGAGGAGAGTGGGGAGGAGCAGAAGGAGGAGGAGCATGGGGAGGAGGAGGTGGAGGAGGAGGAGCATGGGGAGGAGAAGGAGAAGGAGGAGGAGAGTGGGGAGGAGGAGGAGAAGGAGGTGGTGGAGGAAGAAGAGGATGGTTCATGATGAGAGAGTCGAGGCAAGTGATCAAATCAGAAGGTGGAGAGATGAGAGTTGTGTTATCACCAAGAGGAAGGATCATTGAGAAGCCAATGCACATTGGGTTTCTAACAATGGAACCTAAGACCCTCTTTGTCCCTCAGTATCTTGACTCAAGCCTTCTCATCTTTATTCGTCAAGGTGAAGCAACCCTTGGAGTGATATGCAAAGACGAATTCGGAGAGAGGAAACTGAAAGCAGGAGACATCTACTGGATTCCGGCTGGTTCTGTCTTTTATTTACATAACACAGGCCTAGGTCAACGCCTTCATGTCATTTGCAGCATTGATCCAACGCAGAGTCTCGGCTTCGAAACCTTCCAACCTTTCTATATAGGTGGAGGGCCTTCGTCTGTTCTAGCTGGATTTGATCCTCACACTTTAACTTCAGCATTCAACGTTTCCCTACCTGAGCTGCAACAAATGATGATGAGTCAATTCCGTGGCCCGATTGTATACGTCACCGAAGGGCCACAACCACAACCACAATCGACGGTATGGACCCAATTTTTGGGGTTGAGAGGGGAAGAGAAACATAAGCAACTCAAGAAACTCTTGGAGACTAAACAAGGATCCCCTCAAGATCAACAATACAGTTCAGGGTGGTCTTGGAGGAATATTGTAAGATCGATTTTGGATCTAACAGAAGAAAAGAATAAAGGGTCAGGATCTTCTGAATGTGAGGACTCGTACAACATTTATGATAAGAAAGATAAACCAAGTTTTGATAACAAATATGGATGGAGTATAGCTCTTGATTATGACGATTACAAGCCTCTTAAACACTCGGGGATCGGAGTATACCTCGTAAATCTCACCGCGGGAGCAATGATGGCTCCACACATGAACCCAACAGCAACAGAATATGGTATAGTTTTAGCTGGCTCTGGAGAGATCCAAGTTGTATTTCCTAATGGAACTTCTGCTATGAACACAAGAGTCTCCGTAGGAGATGTCTTCTGGATCCCTAGATACTTCGCGTTTTGCCAGATCGCATCTCGGACCGGACCATTCGAGTTTGTAGGATTCACCACATCAGCTCACAAGAATAGACCTCAGTTCCTAGTTGGATCAAACTCACTACTCAGGACTTTGAATCTGACGTCATTGTCCATAGCGTTTGGAGTCGATGAAGAGACAATGAGGCGGTTCATTGAGGCGCAGAGAGAGGCTGTTATTCTCCCTACGCCAGCGGCAGCTCCCCCGCATGTTGGTGAAATGGTTAGCGACCGCTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

511

Amino Acids

55.7

Weight (kDa)

5.83

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 119 - 233 1.6e-06 Cupin
Cupin_1 PF00190 334 - 479 2.9e-34 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1402
AatII GACGTC 1 cut(s) 1400
AccB7I CCANNNNNTGG 1 cut(s) 805
AccI GTMKAC 2 cut(s) 750, 1095
AccII CGCG 2 cut(s) 1115, 1274
AciI CCGC 6 cut(s) 1113, 1115, 1441, 1487, 1499, 1525
AclI AACGTT 1 cut(s) 693
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 1397
AfaI GTAC 2 cut(s) 45, 986
AfiI CCNNNNNNNGG 2 cut(s) 805, 1505
AgsI TTSAA 2 cut(s) 691, 1390
AjuI GAANNNNNNNTTGG 4 cut(s) 432, 464, 1185, 1217
AluBI AGCT 7 cut(s) 20, 656, 709, 1046, 1175, 1334, 1493
AluI AGCT 7 cut(s) 20, 656, 709, 1046, 1175, 1334, 1493
Alw26I GTCTC 5 cut(s) 492, 602, 848, 1240, 1424
Ama87I CYCGRG 1 cut(s) 1081
AoxI GGCC 4 cut(s) 547, 638, 739, 764
ApeKI GCWGC 3 cut(s) 576, 709, 1490
ApoI RAATTY 1 cut(s) 470
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
AspA2I CCTAGG 1 cut(s) 550
AspLEI GCGC 1 cut(s) 1456
AspS9I GGNCC 6 cut(s) 638, 740, 764, 795, 1296, 1301
AsuHPI GGTGA 6 cut(s) 312, 449, 748, 1102, 1315, 1520
AsuII TTCGAA 1 cut(s) 606
AvaI CYCGRG 1 cut(s) 1081
AvaII GGWCC 3 cut(s) 795, 1296, 1301
AvrII CCTAGG 1 cut(s) 550
BamHI GGATCC 2 cut(s) 866, 1257
BbsI GAAGAC 1 cut(s) 1243
BbvI GCAGC 3 cut(s) 588, 696, 1502
BccI CCATC 3 cut(s) 246, 1029, 1120
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 5 cut(s) 492, 602, 848, 1240, 1424
BfaI CTAG 4 cut(s) 551, 653, 1263, 1358
BisI GCNGC 4 cut(s) 577, 710, 1488, 1491
BlnI CCTAGG 1 cut(s) 550
BlsI GCNGC 4 cut(s) 578, 711, 1489, 1492
Bme18I GGWCC 3 cut(s) 795, 1296, 1301
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 6 cut(s) 638, 740, 764, 795, 1296, 1301
BmiI GGNNCC 5 cut(s) 375, 797, 868, 1131, 1259
BmsI GCATC 1 cut(s) 1298
BpiI GAAGAC 1 cut(s) 1243
BplI GAGNNNNNCTC 4 cut(s) 1030, 1062, 1083, 1115
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 705
Bpu14I TTCGAA 1 cut(s) 606
BpuEI CTTGAG 3 cut(s) 394, 824, 858
Bsa29I ATCGAT 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 1397
BsaJI CCNNGG 4 cut(s) 448, 550, 736, 1113
BsaWI WCCGGW 1 cut(s) 1298
Bsc4I CCNNNNNNNGG 2 cut(s) 805, 1505
Bse1I ACTGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 1128
BseCI ATCGAT 1 cut(s) 923
BseDI CCNNGG 4 cut(s) 448, 550, 736, 1113
BseGI GGATG 2 cut(s) 257, 1040
BseLI CCNNNNNNNGG 2 cut(s) 805, 1505
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 4 cut(s) 410, 696, 1364, 1393
BseNI ACTGG 1 cut(s) 512
BseXI GCAGC 3 cut(s) 588, 696, 1502
Bsh1236I CGCG 2 cut(s) 1115, 1274
Bsh1285I CGRYCG 1 cut(s) 1525
BshFI GGCC 4 cut(s) 549, 640, 741, 766
BshVI ATCGAT 1 cut(s) 923
BsiEI CGRYCG 1 cut(s) 1525
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 515, 1299
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 805, 1505
BsmAI GTCTC 5 cut(s) 492, 602, 848, 1240, 1424
BsmFI GGGAC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 4 cut(s) 549, 640, 741, 766
BsoBI CYCGRG 1 cut(s) 1081
Bsp119I TTCGAA 1 cut(s) 606
BspACI CCGC 6 cut(s) 1113, 1115, 1441, 1487, 1499, 1525
BspANI GGCC 4 cut(s) 549, 640, 741, 766
BspCNI CTCAG 4 cut(s) 409, 697, 1363, 1392
BspDI ATCGAT 1 cut(s) 923
BspFNI CGCG 2 cut(s) 1115, 1274
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 5 cut(s) 375, 797, 868, 1131, 1259
BspT104I TTCGAA 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 1128
BsrI ACTGG 1 cut(s) 512
BssECI CCNNGG 4 cut(s) 448, 550, 736, 1113
BssNAI GTATAC 2 cut(s) 751, 1096
BssNI GRCGYC 1 cut(s) 1397
BssT1I CCWWGG 2 cut(s) 448, 550
Bst1107I GTATAC 2 cut(s) 751, 1096
Bst4CI ACNGT 2 cut(s) 790, 890
Bst6I CTCTTC 4 cut(s) 90, 241, 816, 1422
BstACI GRCGYC 1 cut(s) 1397
BstBI TTCGAA 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 1177, 1485
BstDEI CTNAG 5 cut(s) 378, 396, 705, 1350, 1379
BstDSI CCRYGG 2 cut(s) 736, 1113
BstF5I GGATG 2 cut(s) 257, 1040
BstFNI CGCG 2 cut(s) 1115, 1274
BstHHI GCGC 1 cut(s) 1456
BstMAI GTCTC 5 cut(s) 492, 602, 848, 1240, 1424
BstMCI CGRYCG 1 cut(s) 1525
BstMWI GCNNNNNNNGC 1 cut(s) 1127
BstNSI RCATGY 1 cut(s) 1505
BstUI CGCG 2 cut(s) 1115, 1274
BstV1I GCAGC 3 cut(s) 588, 696, 1502
BstV2I GAAGAC 1 cut(s) 1243
BstX2I RGATCY 5 cut(s) 866, 931, 962, 1188, 1257
BstYI RGATCY 5 cut(s) 866, 931, 962, 1188, 1257
BstZ17I GTATAC 2 cut(s) 751, 1096
Bsu15I ATCGAT 1 cut(s) 923
BsuRI GGCC 4 cut(s) 549, 640, 741, 766
BsuTUI ATCGAT 1 cut(s) 923
BtgI CCRYGG 2 cut(s) 736, 1113
BtsCI GGATG 2 cut(s) 257, 1040
Cac8I GCNNGC 2 cut(s) 1177, 1485
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 1456
Cfr13I GGNCC 6 cut(s) 638, 740, 764, 795, 1296, 1301
Cfr42I CCGCGG 1 cut(s) 1116
ClaI ATCGAT 1 cut(s) 923
CpoI CGGWCCG 1 cut(s) 1296
Csp6I GTAC 2 cut(s) 44, 985
CspI CGGWCCG 1 cut(s) 1296
CviAII CATG 6 cut(s) 150, 180, 259, 566, 1138, 1502
CviQI GTAC 2 cut(s) 44, 985
DdeI CTNAG 5 cut(s) 378, 396, 705, 1350, 1379
DrdI GACNNNNNNGTC 1 cut(s) 1402
DseDI GACNNNNNNGTC 1 cut(s) 1402
Eam1104I CTCTTC 4 cut(s) 90, 241, 816, 1422
EarI CTCTTC 4 cut(s) 90, 241, 816, 1422
Eco130I CCWWGG 2 cut(s) 448, 550
Eco147I AGGCCT 1 cut(s) 549
Eco47I GGWCC 3 cut(s) 795, 1296, 1301
Eco57I CTGAAG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 1081
EcoO109I RGGNCCY 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 470
EcoT14I CCWWGG 2 cut(s) 448, 550
ErhI CCWWGG 2 cut(s) 448, 550
FaeI CATG 6 cut(s) 153, 183, 262, 569, 1141, 1505
FalI AAGNNNNNCTT 6 cut(s) 371, 403, 432, 464, 833, 865
FaqI GGGAC 1 cut(s) 377
FatI CATG 6 cut(s) 149, 179, 258, 565, 1137, 1501
FauI CCCGC 2 cut(s) 1108, 1506
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 2 cut(s) 750, 1095
Fnu4HI GCNGC 4 cut(s) 577, 710, 1488, 1491
FokI GGATG 2 cut(s) 264, 1047
Fsp4HI GCNGC 4 cut(s) 577, 710, 1488, 1491
FspBI CTAG 4 cut(s) 551, 653, 1263, 1358
GlaI GCGC 1 cut(s) 1455
GluI GCNGC 4 cut(s) 577, 710, 1488, 1491
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 4 cut(s) 549, 640, 741, 766
HapII CCGG 2 cut(s) 515, 1299
HhaI GCGC 1 cut(s) 1456
Hin1I GRCGYC 1 cut(s) 1397
Hin1II CATG 6 cut(s) 153, 183, 262, 569, 1141, 1505
Hin6I GCGC 1 cut(s) 1454
HinP1I GCGC 1 cut(s) 1454
HincII GTYRAC 1 cut(s) 557
HindII GTYRAC 1 cut(s) 557
HpaII CCGG 2 cut(s) 515, 1299
HphI GGTGA 6 cut(s) 312, 449, 748, 1102, 1315, 1520
Hpy166II GTNNAC 3 cut(s) 557, 751, 1096
Hpy188I TCNGA 7 cut(s) 26, 292, 476, 970, 1091, 1296, 1395
Hpy188III TCNNGA 9 cut(s) 259, 404, 841, 875, 960, 1049, 1182, 1255, 1381
Hpy8I GTNNAC 3 cut(s) 557, 751, 1096
Hpy99I CGWCG 1 cut(s) 790
HpyCH4III ACNGT 2 cut(s) 790, 890
HpyCH4IV ACGT 3 cut(s) 693, 753, 1397
HpyCH4V TGCA 4 cut(s) 352, 462, 576, 712
HpyF10VI GCNNNNNNNGC 1 cut(s) 1127
HpyF3I CTNAG 5 cut(s) 378, 396, 705, 1350, 1379
HpySE526I ACGT 3 cut(s) 693, 753, 1397
Hsp92I GRCGYC 1 cut(s) 1397
Hsp92II CATG 6 cut(s) 153, 183, 262, 569, 1141, 1505
HspAI GCGC 1 cut(s) 1454
Ksp22I TGATCA 1 cut(s) 282
KspI CCGCGG 1 cut(s) 1116
LmnI GCTCC 7 cut(s) 107, 131, 146, 176, 1118, 1135, 1498
Lsp1109I GCAGC 3 cut(s) 588, 696, 1502
LweI GCATC 1 cut(s) 1298
MaeI CTAG 4 cut(s) 551, 653, 1263, 1358
MaeII ACGT 3 cut(s) 693, 753, 1397
MaeIII GTNAC 1 cut(s) 754
MboII GAAGA 9 cut(s) 104, 107, 255, 258, 833, 953, 957, 1243, 1439
MflI RGATCY 5 cut(s) 866, 931, 962, 1188, 1257
MluCI AATT 3 cut(s) 470, 731, 800
MlyI GAGTC 7 cut(s) 278, 401, 604, 736, 974, 1242, 1428
MmeI TCCRAC 3 cut(s) 611, 640, 1342
MseI TTAA 2 cut(s) 677, 1074
MslI CAYNNNNRTG 1 cut(s) 1506
MspA1I CMGCKG 2 cut(s) 1115, 1487
MspI CCGG 2 cut(s) 515, 1299
Mva1269I GAATGC 1 cut(s) 686
MvnI CGCG 2 cut(s) 1115, 1274
MwoI GCNNNNNNNGC 1 cut(s) 1127
NlaIII CATG 6 cut(s) 153, 183, 262, 569, 1141, 1505
NlaIV GGNNCC 5 cut(s) 375, 797, 868, 1131, 1259
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 1 cut(s) 1505
NspV TTCGAA 1 cut(s) 606
PagI TCATGA 1 cut(s) 258
PceI AGGCCT 1 cut(s) 549
PctI GAATGC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 511, 1320, 1390
PflMI CCANNNNNTGG 1 cut(s) 805
PkrI GCNGC 4 cut(s) 578, 711, 1489, 1492
PleI GAGTC 7 cut(s) 277, 401, 603, 735, 974, 1241, 1427
PpsI GAGTC 7 cut(s) 277, 401, 603, 735, 974, 1241, 1427
Psp1406I AACGTT 1 cut(s) 693
PspN4I GGNNCC 5 cut(s) 375, 797, 868, 1131, 1259
PspPI GGNCC 6 cut(s) 638, 740, 764, 795, 1296, 1301
PsuI RGATCY 5 cut(s) 866, 931, 962, 1188, 1257
RsaI GTAC 2 cut(s) 45, 986
RsaNI GTAC 2 cut(s) 44, 985
RseI CAYNNNNRTG 1 cut(s) 1506
Rsr2I CGGWCCG 1 cut(s) 1296
RsrII CGGWCCG 1 cut(s) 1296
SacII CCGCGG 1 cut(s) 1116
SaqAI TTAA 2 cut(s) 677, 1074
SatI GCNGC 4 cut(s) 577, 710, 1488, 1491
Sau96I GGNCC 6 cut(s) 638, 740, 764, 795, 1296, 1301
SchI GAGTC 7 cut(s) 278, 401, 604, 736, 974, 1242, 1428
SfaNI GCATC 1 cut(s) 1298
Sfr303I CCGCGG 1 cut(s) 1116
SfuI TTCGAA 1 cut(s) 606
SgrBI CCGCGG 1 cut(s) 1116
SinI GGWCC 3 cut(s) 795, 1296, 1301
SmiMI CAYNNNNRTG 1 cut(s) 1506
SmlI CTYRAG 3 cut(s) 409, 839, 873
SmoI CTYRAG 3 cut(s) 409, 839, 873
Sse9I AATT 3 cut(s) 470, 731, 800
SseBI AGGCCT 1 cut(s) 549
SsiI CCGC 6 cut(s) 1113, 1115, 1441, 1487, 1499, 1525
SspI AATATT 1 cut(s) 913
SspMI CTAG 4 cut(s) 551, 653, 1263, 1358
StuI AGGCCT 1 cut(s) 549
StyI CCWWGG 2 cut(s) 448, 550
TaaI ACNGT 2 cut(s) 790, 890
TaiI ACGT 3 cut(s) 696, 756, 1400
TaqI TCGA 6 cut(s) 272, 606, 785, 923, 1308, 1422
TasI AATT 3 cut(s) 470, 731, 800
TauI GCSGC 1 cut(s) 1490
TfiI GAWTC 3 cut(s) 511, 1320, 1390
Tru1I TTAA 2 cut(s) 677, 1074
Tru9I TTAA 2 cut(s) 677, 1074
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 3 cut(s) 576, 709, 1490
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 7 cut(s) 99, 247, 554, 1154, 1238, 1435, 1440
TspGWI ACGGA 2 cut(s) 725, 1228
Van91I CCANNNNNTGG 1 cut(s) 805
VpaK11BI GGWCC 3 cut(s) 795, 1296, 1301
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 1505
XmaJI CCTAGG 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 750, 1095
XspI CTAG 4 cut(s) 551, 653, 1263, 1358
ZraI GACGTC 1 cut(s) 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.