MD11G1130400.v1.1

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001754
Physical Location & Seq
Forward (+)
11834159 .. 11837245
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1130400.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGCTGGTGATGTGCTATGGAGTTTCAGTGGCGGTGGGGTACACTGGTGATCAGGATGAAGGTTGGAGGAGAGGGACGACGGAGGAAGAAGATAGGAGGGAGGAGCAGGAAAGCGGTGGGAGGAGGAGGCCGGAAGAGGAGGAGGGGCATTATCCAGAATCGGGAGGGGAAGAATCGGAAGAAGGTTGGTTTTTGCTGCCACAGGCAAAGCAGGTAGTGAAAACTGCAGCAGGAGAGATGAGAGTGGTGATGAGTGCGCGCAGTAGGGTTGTTGATAAGCCCATGCATATCGGGTTTATTACAATGGAGCCTAAGTCTCTCTTTATTCCTCATTATCTCGACTCCAATCTCATCCTCTTTGTCCGCAGAGGAGAAGTAAAGGTGGGGTTGATCTACAAAGATGAATTAGGAGAGAGGAGATTGAAGACAGGGGATGTGTATAGAATTCCAGCTGGGTCTCCATTTTACTTGGTGAACACTGGAGAGGGCCAGAGGGTTCACATCATTTGCAGCATTGACACTTCTGAGAGTTTGGGAATGGGTTTGCAGTCCTTCTTCATTGGTGGAGGAAGCAACCCACAGTCTATTATTGCTGGATTTGACCATGAAATACTTGCAAATGCCTTCAATGTCACATCATCAGAACTAAGAGAGATGTTCACAAGTCAACAACAAGGTCCAATCGTCTTTGTGCCCGAATCTTATTCGCCAAGTGTCTGGTCAAAATTCCTCCAGCTGAAACAGCAAGACAGACTTCAAGAAATGAAGAGGATGTTTGACATCCAACAAGATGATCACCAAGACAAATCAGAAACATGGTCATGGAGGAAGCTCTTGAACTCAGTTTTTGGCGCTGATTCAAACGAAAACAAGAGGAGGCGAGATGACTACGATAAGGGCAAGGGAACAGGAAAATCACCAGACTCTTACAACCTCTACGACAGAAAGCCCGACTTCAGAAATGACTACGGATGGAGCATGCAGCTCGATGATTCTGATTACTCACCGTTGAAACACTCTGGCATCGGCGTTTACCTTGTTAACCTCACCGCTGGGTCAATGATGGCACCGCATGTGAATCCGAGAGCGACAGAATACGGCATTGTGTTGAGAGGCTCCGGAACACTACAGATTGTGTTCCCAAATGGGACACAAGCCATGAATGCCAACATAGGAGAAGGTGATGTATTTTGGGTGCCAAGGTACTTCCCCTTCTGTCAAATCGCATCAAGAACTGGCCCGCTCGAGTTCTTTGGATTCACAACCTCGGCGCGGAAGAACAGGCCTCAGATTTTGGTTGGTGCGGCGTCTGTTCTTCAGGAGCTCAAAGGACCCGAGCTTGCAGCTGCTTTCGGCGTGACTGAAGACCGGTTGAGGAGGTTTGTTGATGCTCAGCGCGAGGCAGTGATCTTGCCTACGGCACAAGCAGCACCGCCGTACAAGGAGGAGAGGAAGCCGCCGAAGGAGGAGGAGGAAGAGAGGAGGCAGCAGCATCCGGAAGAGGGCGAGAGAAGGGAGGGCGAGAGAAGGGGGGATGCGGGAAGGGGCGAGGAGATAAGGGAGGATGACAGAAAGGATAAGGGGAGGTTTGAGAGGGTGCCAGAAGTGATCAAGAGTGTTGGGAATGACATAGTTATGGGTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

550

Amino Acids

62.15

Weight (kDa)

5.35

Isoelectric Point (pI)

63.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 95 - 215 2e-09 Cupin
Cupin_1 PF00190 310 - 455 1.4e-32 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 3 cut(s) 1066, 1195, 1597
AccBSI CCGCTC 1 cut(s) 1243
AccII CGCG 3 cut(s) 259, 1273, 1398
AccIII TCCGGA 2 cut(s) 1118, 1495
AcsI RAATTY 2 cut(s) 444, 725
AcuI CTGAAG 3 cut(s) 940, 1301, 1383
AcyI GRCGYC 1 cut(s) 1307
AfaI GTAC 3 cut(s) 41, 1205, 1439
AfiI CCNNNNNNNGG 3 cut(s) 161, 1272, 1502
AgeI ACCGGT 1 cut(s) 1368
AgsI TTSAA 6 cut(s) 424, 628, 758, 838, 861, 1012
AluBI AGCT 7 cut(s) 452, 736, 832, 985, 1324, 1339, 1346
AluI AGCT 7 cut(s) 452, 736, 832, 985, 1324, 1339, 1346
Alw21I GWGCWC 1 cut(s) 1326
Alw26I GTCTC 2 cut(s) 321, 462
Ama87I CYCGRG 2 cut(s) 1244, 1334
Aor13HI TCCGGA 2 cut(s) 1118, 1495
AoxI GGCC 4 cut(s) 128, 487, 1237, 1283
ApeKI GCWGC 9 cut(s) 196, 227, 510, 982, 1343, 1346, 1427, 1486, 1489
ApoI RAATTY 2 cut(s) 444, 725
AsiGI ACCGGT 1 cut(s) 1368
AspLEI GCGC 5 cut(s) 259, 261, 854, 1273, 1398
AspS9I GGNCC 4 cut(s) 487, 677, 1238, 1331
AsuHPI GGTGA 9 cut(s) 19, 59, 259, 484, 788, 909, 996, 1039, 1193
AvaI CYCGRG 2 cut(s) 1244, 1334
AvaII GGWCC 2 cut(s) 677, 1331
BaeGI GKGCMC 1 cut(s) 696
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanI GGYRCC 3 cut(s) 1066, 1195, 1597
BanII GRGCYC 1 cut(s) 1326
BarI GAAGNNNNNNTAC 2 cut(s) 1196, 1228
BbsI GAAGAC 2 cut(s) 431, 1371
Bbv12I GWGCWC 1 cut(s) 1326
BbvI GCAGC 9 cut(s) 183, 239, 522, 994, 1333, 1355, 1439, 1498, 1501
BccI CCATC 2 cut(s) 966, 1057
BceAI ACGGC 3 cut(s) 1114, 1420, 1434
BcgI CGANNNNNNTGC 2 cut(s) 967, 1001
BclI TGATCA 3 cut(s) 49, 793, 1608
BcoDI GTCTC 2 cut(s) 321, 462
BfmI CTRYAG 2 cut(s) 225, 1127
BfoI RGCGCY 1 cut(s) 855
BfuAI ACCTGC 1 cut(s) 202
BlpI GCTNAGC 1 cut(s) 1392
Bme18I GGWCC 2 cut(s) 677, 1331
BmeT110I CYCGRG 2 cut(s) 1244, 1334
BmgT120I GGNCC 4 cut(s) 487, 677, 1238, 1331
BmiI GGNNCC 6 cut(s) 309, 1068, 1117, 1197, 1333, 1599
BmsI GCATC 5 cut(s) 1032, 1235, 1378, 1501, 1525
BpiI GAAGAC 2 cut(s) 431, 1371
BpmI CTGGAG 2 cut(s) 501, 716
Bpu1102I GCTNAGC 1 cut(s) 1392
BsaHI GRCGYC 1 cut(s) 1307
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 2 cut(s) 1199, 1266
BsaWI WCCGGW 3 cut(s) 1118, 1368, 1495
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 1272, 1502
Bse118I RCCGGY 1 cut(s) 1368
Bse1I ACTGG 3 cut(s) 49, 484, 1240
BseAI TCCGGA 2 cut(s) 1118, 1495
BseDI CCNNGG 2 cut(s) 1199, 1266
BseGI GGATG 9 cut(s) 61, 351, 439, 777, 780, 977, 1492, 1540, 1570
BseLI CCNNNNNNNGG 3 cut(s) 161, 1272, 1502
BseMII CTCAG 4 cut(s) 516, 855, 1301, 1406
BseNI ACTGG 3 cut(s) 49, 484, 1240
BsePI GCGCGC 1 cut(s) 257
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 9 cut(s) 183, 239, 522, 994, 1333, 1355, 1439, 1498, 1501
BseYI CCCAGC 2 cut(s) 452, 1052
Bsh1236I CGCG 3 cut(s) 259, 1273, 1398
BshFI GGCC 4 cut(s) 130, 489, 1239, 1285
BshNI GGYRCC 3 cut(s) 1066, 1195, 1597
BshTI ACCGGT 1 cut(s) 1368
BsiHKAI GWGCWC 1 cut(s) 1326
BsiHKCI CYCGRG 2 cut(s) 1244, 1334
BsiSI CCGG 4 cut(s) 131, 1119, 1369, 1496
BslFI GGGAC 2 cut(s) 88, 1162
BslI CCNNNNNNNGG 3 cut(s) 161, 1272, 1502
BsmAI GTCTC 2 cut(s) 321, 462
BsmFI GGGAC 2 cut(s) 88, 1162
BsmI GAATGC 1 cut(s) 1168
BsnI GGCC 4 cut(s) 130, 489, 1239, 1285
Bso31I GGTCTC 1 cut(s) 462
BsoBI CYCGRG 2 cut(s) 1244, 1334
Bsp1286I GDGCHC 2 cut(s) 696, 1326
Bsp13I TCCGGA 2 cut(s) 1118, 1495
Bsp143I GATC 5 cut(s) 49, 390, 793, 1407, 1608
Bsp1720I GCTNAGC 1 cut(s) 1392
BspANI GGCC 4 cut(s) 130, 489, 1239, 1285
BspCNI CTCAG 4 cut(s) 517, 854, 1300, 1405
BspEI TCCGGA 2 cut(s) 1118, 1495
BspFNI CGCG 3 cut(s) 259, 1273, 1398
BspLI GGNNCC 6 cut(s) 309, 1068, 1117, 1197, 1333, 1599
BspMAI CTGCAG 1 cut(s) 229
BspMI ACCTGC 1 cut(s) 202
BspT107I GGYRCC 3 cut(s) 1066, 1195, 1597
BspTNI GGTCTC 1 cut(s) 462
BsrBI CCGCTC 1 cut(s) 1243
BsrFI RCCGGY 1 cut(s) 1368
BsrI ACTGG 3 cut(s) 49, 484, 1240
BssAI RCCGGY 1 cut(s) 1368
BssECI CCNNGG 2 cut(s) 1199, 1266
BssHII GCGCGC 1 cut(s) 257
BssMI GATC 5 cut(s) 49, 390, 793, 1407, 1608
BssNI GRCGYC 1 cut(s) 1307
BssT1I CCWWGG 1 cut(s) 1199
Bst4CI ACNGT 2 cut(s) 582, 1008
Bst6I CTCTTC 4 cut(s) 129, 761, 1470, 1494
BstACI GRCGYC 1 cut(s) 1307
BstC8I GCNNGC 4 cut(s) 259, 980, 1241, 1341
BstDEI CTNAG 6 cut(s) 312, 525, 647, 841, 1287, 1392
BstF5I GGATG 9 cut(s) 61, 351, 439, 777, 780, 977, 1492, 1540, 1570
BstFNI CGCG 3 cut(s) 259, 1273, 1398
BstH2I RGCGCY 1 cut(s) 855
BstHHI GCGC 5 cut(s) 259, 261, 854, 1273, 1398
BstKTI GATC 5 cut(s) 52, 393, 796, 1410, 1611
BstMAI GTCTC 2 cut(s) 321, 462
BstMBI GATC 5 cut(s) 49, 390, 793, 1407, 1608
BstMWI GCNNNNNNNGC 3 cut(s) 742, 1163, 1427
BstNSI RCATGY 2 cut(s) 982, 1076
BstSFI CTRYAG 2 cut(s) 225, 1127
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 3 cut(s) 259, 1273, 1398
BstV1I GCAGC 9 cut(s) 183, 239, 522, 994, 1333, 1355, 1439, 1498, 1501
BstV2I GAAGAC 2 cut(s) 431, 1371
BstXI CCANNNNNNTGG 1 cut(s) 717
BsuRI GGCC 4 cut(s) 130, 489, 1239, 1285
BtsCI GGATG 9 cut(s) 61, 351, 439, 777, 780, 977, 1492, 1540, 1570
BtsI GCAGTG 1 cut(s) 1410
BtsIMutI CAGTG 4 cut(s) 33, 42, 477, 1410
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 4 cut(s) 259, 980, 1241, 1341
CfoI GCGC 5 cut(s) 259, 261, 854, 1273, 1398
Cfr10I RCCGGY 1 cut(s) 1368
Cfr13I GGNCC 4 cut(s) 487, 677, 1238, 1331
CseI GACGC 1 cut(s) 1296
Csp6I GTAC 3 cut(s) 40, 1204, 1438
CspAI ACCGGT 1 cut(s) 1368
CviAII CATG 7 cut(s) 283, 605, 816, 822, 979, 1073, 1159
CviQI GTAC 3 cut(s) 40, 1204, 1438
DdeI CTNAG 6 cut(s) 312, 525, 647, 841, 1287, 1392
DpnI GATC 5 cut(s) 51, 392, 795, 1409, 1610
DpnII GATC 5 cut(s) 49, 390, 793, 1407, 1608
Eam1104I CTCTTC 4 cut(s) 129, 761, 1470, 1494
EarI CTCTTC 4 cut(s) 129, 761, 1470, 1494
Ecl136II GAGCTC 1 cut(s) 1324
Eco130I CCWWGG 1 cut(s) 1199
Eco147I AGGCCT 1 cut(s) 1285
Eco24I GRGCYC 1 cut(s) 1326
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 677, 1331
Eco53kI GAGCTC 1 cut(s) 1324
Eco57I CTGAAG 3 cut(s) 940, 1301, 1383
Eco88I CYCGRG 2 cut(s) 1244, 1334
EcoICRI GAGCTC 1 cut(s) 1324
EcoO109I RGGNCCY 1 cut(s) 1331
EcoRI GAATTC 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 1199
EcoT22I ATGCAT 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 1326
ErhI CCWWGG 1 cut(s) 1199
FaeI CATG 7 cut(s) 286, 608, 819, 825, 982, 1076, 1162
FalI AAGNNNNNCTT 6 cut(s) 305, 337, 738, 770, 938, 970
FaqI GGGAC 2 cut(s) 88, 1162
FatI CATG 7 cut(s) 282, 604, 815, 821, 978, 1072, 1158
FauI CCCGC 2 cut(s) 1248, 1531
FbaI TGATCA 3 cut(s) 49, 793, 1608
FokI GGATG 9 cut(s) 68, 338, 446, 767, 784, 984, 1479, 1547, 1577
FriOI GRGCYC 1 cut(s) 1326
GlaI GCGC 5 cut(s) 258, 260, 853, 1272, 1397
GsaI CCCAGC 2 cut(s) 456, 1056
GsuI CTGGAG 2 cut(s) 501, 716
HaeII RGCGCY 1 cut(s) 855
HaeIII GGCC 4 cut(s) 130, 489, 1239, 1285
HapII CCGG 4 cut(s) 131, 1119, 1369, 1496
HgaI GACGC 1 cut(s) 1296
HhaI GCGC 5 cut(s) 259, 261, 854, 1273, 1398
Hin1I GRCGYC 1 cut(s) 1307
Hin1II CATG 7 cut(s) 286, 608, 819, 825, 982, 1076, 1162
Hin6I GCGC 5 cut(s) 257, 259, 852, 1271, 1396
HinP1I GCGC 5 cut(s) 257, 259, 852, 1271, 1396
HincII GTYRAC 2 cut(s) 668, 1042
HindII GTYRAC 2 cut(s) 668, 1042
HinfI GANTC 9 cut(s) 158, 173, 341, 698, 857, 923, 992, 1078, 1257
HpaI GTTAAC 1 cut(s) 1042
HpaII CCGG 4 cut(s) 131, 1119, 1369, 1496
HphI GGTGA 9 cut(s) 19, 59, 259, 484, 788, 909, 996, 1039, 1193
Hpy166II GTNNAC 7 cut(s) 42, 475, 499, 660, 668, 1033, 1042
Hpy188I TCNGA 8 cut(s) 178, 526, 643, 811, 959, 997, 1083, 1290
Hpy8I GTNNAC 7 cut(s) 42, 475, 499, 660, 668, 1033, 1042
Hpy99I CGWCG 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 582, 1008
HpyCH4V TGCA 7 cut(s) 227, 286, 510, 547, 617, 982, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 742, 1163, 1427
HpyF3I CTNAG 6 cut(s) 312, 525, 647, 841, 1287, 1392
Hsp92I GRCGYC 1 cut(s) 1307
Hsp92II CATG 7 cut(s) 286, 608, 819, 825, 982, 1076, 1162
HspAI GCGC 5 cut(s) 257, 259, 852, 1271, 1396
Kpn2I TCCGGA 2 cut(s) 1118, 1495
Ksp22I TGATCA 3 cut(s) 49, 793, 1608
KspAI GTTAAC 1 cut(s) 1042
Kzo9I GATC 5 cut(s) 49, 390, 793, 1407, 1608
LmnI GCTCC 5 cut(s) 103, 307, 975, 1121, 1321
Lsp1109I GCAGC 9 cut(s) 183, 239, 522, 994, 1333, 1355, 1439, 1498, 1501
LweI GCATC 5 cut(s) 1032, 1235, 1378, 1501, 1525
MaeIII GTNAC 2 cut(s) 631, 1357
MalI GATC 5 cut(s) 51, 392, 795, 1409, 1610
MbiI CCGCTC 1 cut(s) 1243
MboI GATC 5 cut(s) 49, 390, 793, 1407, 1608
MhlI GDGCHC 2 cut(s) 696, 1326
MluCI AATT 3 cut(s) 404, 444, 725
MlyI GAGTC 2 cut(s) 335, 917
MmeI TCCRAC 2 cut(s) 44, 808
Mph1103I ATGCAT 1 cut(s) 288
MroI TCCGGA 2 cut(s) 1118, 1495
MseI TTAA 1 cut(s) 1041
MslI CAYNNNNRTG 2 cut(s) 820, 1634
MspA1I CMGCKG 4 cut(s) 452, 736, 1052, 1346
MspI CCGG 4 cut(s) 131, 1119, 1369, 1496
Mva1269I GAATGC 1 cut(s) 1168
MvnI CGCG 3 cut(s) 259, 1273, 1398
MwoI GCNNNNNNNGC 3 cut(s) 742, 1163, 1427
NdeII GATC 5 cut(s) 49, 390, 793, 1407, 1608
NlaIII CATG 7 cut(s) 286, 608, 819, 825, 982, 1076, 1162
NlaIV GGNNCC 6 cut(s) 309, 1068, 1117, 1197, 1333, 1599
NmeAIII GCCGAG 1 cut(s) 1247
NmuCI GTSAC 2 cut(s) 631, 1357
NsiI ATGCAT 1 cut(s) 288
NspI RCATGY 2 cut(s) 982, 1076
PaeI GCATGC 1 cut(s) 982
PaeR7I CTCGAG 1 cut(s) 1244
PauI GCGCGC 1 cut(s) 257
PceI AGGCCT 1 cut(s) 1285
PctI GAATGC 1 cut(s) 1168
PfeI GAWTC 7 cut(s) 158, 173, 698, 857, 992, 1078, 1257
PinAI ACCGGT 1 cut(s) 1368
PleI GAGTC 2 cut(s) 335, 917
PpsI GAGTC 2 cut(s) 335, 917
PpuMI RGGWCCY 1 cut(s) 1331
Psp124BI GAGCTC 1 cut(s) 1326
Psp5II RGGWCCY 1 cut(s) 1331
PspFI CCCAGC 2 cut(s) 452, 1052
PspN4I GGNNCC 6 cut(s) 309, 1068, 1117, 1197, 1333, 1599
PspPI GGNCC 4 cut(s) 487, 677, 1238, 1331
PspPPI RGGWCCY 1 cut(s) 1331
PspXI VCTCGAGB 1 cut(s) 1244
PstI CTGCAG 1 cut(s) 229
PteI GCGCGC 1 cut(s) 257
PvuII CAGCTG 3 cut(s) 452, 736, 1346
RsaI GTAC 3 cut(s) 41, 1205, 1439
RsaNI GTAC 3 cut(s) 40, 1204, 1438
RseI CAYNNNNRTG 2 cut(s) 820, 1634
SacI GAGCTC 1 cut(s) 1326
SaqAI TTAA 1 cut(s) 1041
Sau3AI GATC 5 cut(s) 49, 390, 793, 1407, 1608
Sau96I GGNCC 4 cut(s) 487, 677, 1238, 1331
SchI GAGTC 2 cut(s) 335, 917
SduI GDGCHC 2 cut(s) 696, 1326
SfaNI GCATC 5 cut(s) 1032, 1235, 1378, 1501, 1525
SfcI CTRYAG 2 cut(s) 225, 1127
Sfr274I CTCGAG 1 cut(s) 1244
SinI GGWCC 2 cut(s) 677, 1331
SlaI CTCGAG 1 cut(s) 1244
SmiMI CAYNNNNRTG 2 cut(s) 820, 1634
SmlI CTYRAG 1 cut(s) 1244
SmoI CTYRAG 1 cut(s) 1244
SphI GCATGC 1 cut(s) 982
Sse9I AATT 3 cut(s) 404, 444, 725
SseBI AGGCCT 1 cut(s) 1285
SstI GAGCTC 1 cut(s) 1326
StuI AGGCCT 1 cut(s) 1285
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 2 cut(s) 582, 1008
TaqI TCGA 3 cut(s) 339, 987, 1245
TasI AATT 3 cut(s) 404, 444, 725
TauI GCSGC 2 cut(s) 1307, 1459
TfiI GAWTC 7 cut(s) 158, 173, 698, 857, 992, 1078, 1257
Tru1I TTAA 1 cut(s) 1041
Tru9I TTAA 1 cut(s) 1041
TscAI CASTG 4 cut(s) 33, 49, 484, 1410
TseFI GTSAC 2 cut(s) 631, 1357
TseI GCWGC 9 cut(s) 196, 227, 510, 982, 1343, 1346, 1427, 1486, 1489
Tsp45I GTSAC 2 cut(s) 631, 1357
TspDTI ATGAA 6 cut(s) 72, 417, 547, 621, 779, 1175
TspGWI ACGGA 2 cut(s) 95, 984
TspRI CASTG 4 cut(s) 33, 49, 484, 1410
VpaK11BI GGWCC 2 cut(s) 677, 1331
XapI RAATTY 2 cut(s) 444, 725
XceI RCATGY 2 cut(s) 982, 1076
XhoI CTCGAG 1 cut(s) 1244
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.