Rh5CG431400

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001754
Physical Location & Seq
Reverse (-)
59677167 .. 59678994
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG431400.1

Sequence Viewer

Length: 1350 bp
ATGGCTATGAGCTTCGATCACGAAGACGAGGACTGGCGAAGGGATCATAGGGAAGAAGAGTGGGAAGGGCACAGAAGGCATCACCATTGGTTCCTTTTGCAACGATCTAGACTACTGCTGAGAACTGAAGCTGGGGAAATGAGGGTGGTGAGGAGTGTAGGTCAGAGAATGGTTGATAGACATATAAATGTTGGGTTTCTCTCCATGGAACCCAACTCTCTTTTCATCCCTCAGTACCTTGACTCCAGCGTGGTCCTCTTTGTCCACACAGGGCAAGCAAATGTGGGATTGGTAAGCCAACATGAACTCGGAGAGAGGCAATTGAAGGCTGGGGATTTGTACAGAATCCCAGCTGGTTCAACATTTTACTTGCAAAATGTTGGGGAGGGCCAGAGACTTGAGCTCATTCTCAGCATTGACACATCTGATAGTTTAAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCTACCCAAAATCTATCCTTGCTGGGTTCGATTCTCACGTACTCACAAGTGCATTTAACGTCTCATCTTCAGAACTAAAACACATTTTGACAAGGCAACAAGAGGGTCCAATTGTATCTCTGAATAATTCTCAATCATCATCAAACCTTTGGACAAAATTCCTAAGCATGAAAGAGCAGGACAGACTACAAGAACTAAAGAAAATGGTCGACTTCCCACAAGAACAAGAGCAAACCCAAACATGGTCATGGAGGAAACTCTTGACCTCTATGTTCGGAACAATCGAAAACGAGAAGAGGAAGCAAAATCATCATGAGCACTCCCAGAAGGCTTACAACCTCTATGCGAGGAGGCCGGATTTCAAGAACAACTATGGATCGAGCTTGTCAGTTGATGAGTCTGATTACTCACCGCTACAAAACTCCGGCATTGGCGTTTACCTTGTCAATCTTAACGCAGGATCGATGTTGGCACCACATGTGAACCCAGCTGCAACAGAATACGGCGTCGTTCTAAGAGGCTCAGGATCACTCCAGATTGTGTTTCCAAACGGAACCTTGGCAATGAAGGCGAAACTAAAACCAGGGAGAGTGTTCTGGGTTCCGAGGTACTTCCCATTTGTCCAGGTGGCATCTAACAACGAGCAACTTCAGATTTTCGGGTTCACTACCTCAAAGCAGCCGAACCACCCACAATTTCTGGCTGGTTCTAGCTCGGTGCTTGAAGCTCTAAGAGGTCCGGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCGTTTTGTTAATGCTCAGCGCGAAGCTGTAATCTTACCTTACAATGCGGTGAGGCATCATGAGACACATGGGATCCCCATGGCGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

449

Amino Acids

50.99

Weight (kDa)

7.06

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 58 - 183 7.4e-10 Cupin
Cupin_1 PF00190 270 - 415 9.3e-31 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 946
AccI GTMKAC 1 cut(s) 684
AccII CGCG 1 cut(s) 1278
AccIII TCCGGA 1 cut(s) 1213
AciI CCGC 3 cut(s) 887, 1304, 1340
AclWI GGATC 6 cut(s) 51, 859, 943, 1009, 1324, 1337
AcsI RAATTY 2 cut(s) 454, 632
AcuI CTGAAG 3 cut(s) 147, 528, 1109
AcyI GRCGYC 1 cut(s) 981
AfaI GTAC 5 cut(s) 236, 341, 445, 516, 1085
AfiI CCNNNNNNNGG 2 cut(s) 717, 1339
AflIII ACRYGT 1 cut(s) 952
AgsI TTSAA 5 cut(s) 325, 360, 838, 1199, 1255
AjnI CCWGG 2 cut(s) 1057, 1098
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw21I GWGCWC 2 cut(s) 405, 795
Alw26I GTCTC 3 cut(s) 388, 541, 1313
AlwI GGATC 6 cut(s) 51, 859, 943, 1009, 1324, 1337
Aor13HI TCCGGA 1 cut(s) 1213
AoxI GGCC 2 cut(s) 388, 827
ApeKI GCWGC 4 cut(s) 965, 1153, 1223, 1226
ApoI RAATTY 2 cut(s) 454, 632
Asp700I GAANNNNTTC 1 cut(s) 458
AspLEI GCGC 1 cut(s) 1278
AspS9I GGNCC 4 cut(s) 253, 388, 581, 1211
AsuHPI GGTGA 4 cut(s) 74, 160, 876, 1318
AvaII GGWCC 3 cut(s) 253, 581, 1211
BaeGI GKGCMC 1 cut(s) 72
BamHI GGATCC 1 cut(s) 1329
BanI GGYRCC 1 cut(s) 946
BanII GRGCYC 1 cut(s) 405
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 405, 795
BbvI GCAGC 4 cut(s) 952, 1165, 1213, 1235
BceAI ACGGC 1 cut(s) 994
BciT130I CCWGG 2 cut(s) 1059, 1100
BcoDI GTCTC 3 cut(s) 388, 541, 1313
BfaI CTAG 2 cut(s) 108, 1185
BisI GCNGC 4 cut(s) 966, 1154, 1224, 1227
BlpI GCTNAGC 1 cut(s) 1272
BlsI GCNGC 4 cut(s) 967, 1155, 1225, 1228
Bme1390I CCNGG 2 cut(s) 1059, 1100
Bme18I GGWCC 3 cut(s) 253, 581, 1211
BmgT120I GGNCC 4 cut(s) 253, 388, 581, 1211
BmiI GGNNCC 8 cut(s) 92, 210, 475, 582, 948, 1030, 1077, 1331
BmrFI CCNGG 2 cut(s) 1059, 1100
BmsI GCATC 3 cut(s) 88, 1115, 1321
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 2 cut(s) 229, 992
Bpu10I CCTNAGC 2 cut(s) 638, 997
Bpu1102I GCTNAGC 1 cut(s) 1272
BpuEI CTTGAG 1 cut(s) 419
Bsa29I ATCGAT 1 cut(s) 938
BsaAI YACGTR 1 cut(s) 514
BsaHI GRCGYC 1 cut(s) 981
BsaJI CCNNGG 5 cut(s) 204, 1032, 1058, 1079, 1335
BsaWI WCCGGW 1 cut(s) 1213
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 717, 1339
Bse1I ACTGG 2 cut(s) 38, 1349
Bse3DI GCAATG 1 cut(s) 1044
BseAI TCCGGA 1 cut(s) 1213
BseBI CCWGG 2 cut(s) 1059, 1100
BseCI ATCGAT 1 cut(s) 938
BseDI CCNNGG 5 cut(s) 204, 1032, 1058, 1079, 1335
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 717, 1339
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 5 cut(s) 110, 245, 424, 1011, 1286
BseNI ACTGG 2 cut(s) 38, 1349
BseRI GAGGAG 2 cut(s) 166, 838
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 4 cut(s) 952, 1165, 1213, 1235
BseYI CCCAGC 5 cut(s) 131, 329, 349, 497, 961
Bsh1236I CGCG 1 cut(s) 1278
BshFI GGCC 2 cut(s) 390, 829
BshNI GGYRCC 1 cut(s) 946
BshVI ATCGAT 1 cut(s) 938
BsiHKAI GWGCWC 2 cut(s) 405, 795
BsiSI CCGG 3 cut(s) 830, 900, 1214
BslI CCNNNNNNNGG 2 cut(s) 717, 1339
BsmAI GTCTC 3 cut(s) 388, 541, 1313
BsmBI CGTCTC 1 cut(s) 541
BsnI GGCC 2 cut(s) 390, 829
Bsp1286I GDGCHC 3 cut(s) 72, 405, 795
Bsp13I TCCGGA 1 cut(s) 1213
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
Bsp1720I GCTNAGC 1 cut(s) 1272
Bsp19I CCATGG 2 cut(s) 204, 1335
BspACI CCGC 3 cut(s) 887, 1304, 1340
BspANI GGCC 2 cut(s) 390, 829
BspCNI CTCAG 5 cut(s) 111, 244, 423, 1010, 1285
BspDI ATCGAT 1 cut(s) 938
BspEI TCCGGA 1 cut(s) 1213
BspFNI CGCG 1 cut(s) 1278
BspHI TCATGA 2 cut(s) 787, 1315
BspLI GGNNCC 8 cut(s) 92, 210, 475, 582, 948, 1030, 1077, 1331
BspPI GGATC 6 cut(s) 51, 859, 943, 1009, 1324, 1337
BspT107I GGYRCC 1 cut(s) 946
BsrDI GCAATG 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 2 cut(s) 38, 1349
BssECI CCNNGG 5 cut(s) 204, 1032, 1058, 1079, 1335
BssMI GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
BssNI GRCGYC 1 cut(s) 981
BssT1I CCWWGG 3 cut(s) 204, 1032, 1335
Bst2UI CCWGG 2 cut(s) 1059, 1100
Bst6I CTCTTC 2 cut(s) 51, 764
BstACI GRCGYC 1 cut(s) 981
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 514
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 8 cut(s) 119, 231, 410, 638, 989, 997, 1205, 1272
BstDSI CCRYGG 2 cut(s) 204, 1335
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 1 cut(s) 1278
BstHHI GCGC 1 cut(s) 1278
BstKTI GATC 7 cut(s) 19, 46, 107, 854, 938, 1004, 1332
BstMAI GTCTC 3 cut(s) 388, 541, 1313
BstMBI GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
BstMWI GCNNNNNNNGC 2 cut(s) 76, 1043
BstNI CCWGG 2 cut(s) 1059, 1100
BstNSI RCATGY 1 cut(s) 956
BstSCI CCNGG 2 cut(s) 1057, 1098
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 1 cut(s) 1278
BstV1I GCAGC 4 cut(s) 952, 1165, 1213, 1235
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 1329
BstYI RGATCY 1 cut(s) 1329
Bsu15I ATCGAT 1 cut(s) 938
BsuRI GGCC 2 cut(s) 390, 829
BsuTUI ATCGAT 1 cut(s) 938
BtgI CCRYGG 2 cut(s) 204, 1335
BtsCI GGATG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 276
CciI TCATGA 2 cut(s) 787, 1315
CfoI GCGC 1 cut(s) 1278
Cfr13I GGNCC 4 cut(s) 253, 388, 581, 1211
ClaI ATCGAT 1 cut(s) 938
CseI GACGC 1 cut(s) 970
Csp6I GTAC 5 cut(s) 235, 340, 444, 515, 1084
CviQI GTAC 5 cut(s) 235, 340, 444, 515, 1084
DdeI CTNAG 8 cut(s) 119, 231, 410, 638, 989, 997, 1205, 1272
DpnI GATC 7 cut(s) 18, 45, 106, 853, 937, 1003, 1331
DpnII GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
Eam1104I CTCTTC 2 cut(s) 51, 764
EarI CTCTTC 2 cut(s) 51, 764
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 3 cut(s) 204, 1032, 1335
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 3 cut(s) 253, 581, 1211
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 3 cut(s) 147, 528, 1109
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 1057, 1098
EcoT14I CCWWGG 3 cut(s) 204, 1032, 1335
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 3 cut(s) 204, 1032, 1335
Esp3I CGTCTC 1 cut(s) 541
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 4 cut(s) 966, 1154, 1224, 1227
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 4 cut(s) 966, 1154, 1224, 1227
FspBI CTAG 2 cut(s) 108, 1185
GlaI GCGC 1 cut(s) 1277
GluI GCNGC 4 cut(s) 966, 1154, 1224, 1227
GsaI CCCAGC 5 cut(s) 135, 333, 353, 501, 965
GsuI CTGGAG 2 cut(s) 229, 992
HaeIII GGCC 2 cut(s) 390, 829
HapII CCGG 3 cut(s) 830, 900, 1214
HgaI GACGC 1 cut(s) 970
HhaI GCGC 1 cut(s) 1278
Hin1I GRCGYC 1 cut(s) 981
Hin6I GCGC 1 cut(s) 1276
HinP1I GCGC 1 cut(s) 1276
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 5 cut(s) 242, 345, 506, 872, 1255
HpaII CCGG 3 cut(s) 830, 900, 1214
HphI GGTGA 4 cut(s) 74, 160, 876, 1318
Hpy166II GTNNAC 5 cut(s) 265, 685, 913, 958, 1140
Hpy188I TCNGA 9 cut(s) 165, 311, 427, 547, 597, 752, 877, 1080, 1128
Hpy188III TCNNGA 9 cut(s) 20, 108, 736, 788, 838, 999, 1009, 1214, 1316
Hpy8I GTNNAC 5 cut(s) 265, 685, 913, 958, 1140
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 7 cut(s) 33, 59, 69, 319, 796, 1036, 1242
HpyCH4IV ACGT 2 cut(s) 513, 534
HpyCH4V TGCA 5 cut(s) 100, 373, 527, 968, 1223
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 1043
HpyF3I CTNAG 8 cut(s) 119, 231, 410, 638, 989, 997, 1205, 1272
HpySE526I ACGT 2 cut(s) 513, 534
Hsp92I GRCGYC 1 cut(s) 981
HspAI GCGC 1 cut(s) 1276
Kpn2I TCCGGA 1 cut(s) 1213
Kzo9I GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
Lsp1109I GCAGC 4 cut(s) 952, 1165, 1213, 1235
LweI GCATC 3 cut(s) 88, 1115, 1321
MaeI CTAG 2 cut(s) 108, 1185
MaeII ACGT 2 cut(s) 513, 534
MalI GATC 7 cut(s) 18, 45, 106, 853, 937, 1003, 1331
MboI GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
MboII GAAGA 6 cut(s) 35, 65, 68, 451, 534, 781
MfeI CAATTG 2 cut(s) 320, 585
MflI RGATCY 1 cut(s) 1329
MhlI GDGCHC 3 cut(s) 72, 405, 795
MluCI AATT 6 cut(s) 320, 454, 585, 601, 632, 1169
MlyI GAGTC 2 cut(s) 236, 881
MroI TCCGGA 1 cut(s) 1213
MroXI GAANNNNTTC 1 cut(s) 458
MseI TTAA 4 cut(s) 434, 531, 927, 1266
MslI CAYNNNNRTG 2 cut(s) 186, 721
MspA1I CMGCKG 3 cut(s) 353, 965, 1226
MspI CCGG 3 cut(s) 830, 900, 1214
MspR9I CCNGG 2 cut(s) 1059, 1100
MunI CAATTG 2 cut(s) 320, 585
MvaI CCWGG 2 cut(s) 1059, 1100
MvnI CGCG 1 cut(s) 1278
MwoI GCNNNNNNNGC 2 cut(s) 76, 1043
NcoI CCATGG 2 cut(s) 204, 1335
NdeII GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
NlaIV GGNNCC 8 cut(s) 92, 210, 475, 582, 948, 1030, 1077, 1331
NspI RCATGY 1 cut(s) 956
PagI TCATGA 2 cut(s) 787, 1315
PciI ACATGT 1 cut(s) 952
PcsI WCGNNNNNNNCGW 2 cut(s) 510, 756
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 3 cut(s) 345, 506, 1255
PkrI GCNGC 4 cut(s) 967, 1155, 1225, 1228
PleI GAGTC 2 cut(s) 236, 880
PpsI GAGTC 2 cut(s) 236, 880
Ppu21I YACGTR 1 cut(s) 514
PscI ACATGT 1 cut(s) 952
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 1057, 1098
PspFI CCCAGC 5 cut(s) 131, 329, 349, 497, 961
PspGI CCWGG 2 cut(s) 1057, 1098
PspN4I GGNNCC 8 cut(s) 92, 210, 475, 582, 948, 1030, 1077, 1331
PspPI GGNCC 4 cut(s) 253, 388, 581, 1211
PsuI RGATCY 1 cut(s) 1329
PvuII CAGCTG 3 cut(s) 353, 965, 1226
RsaI GTAC 5 cut(s) 236, 341, 445, 516, 1085
RsaNI GTAC 5 cut(s) 235, 340, 444, 515, 1084
RseI CAYNNNNRTG 2 cut(s) 186, 721
SacI GAGCTC 1 cut(s) 405
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 4 cut(s) 434, 531, 927, 1266
SatI GCNGC 4 cut(s) 966, 1154, 1224, 1227
Sau3AI GATC 7 cut(s) 16, 43, 104, 851, 935, 1001, 1329
Sau96I GGNCC 4 cut(s) 253, 388, 581, 1211
SchI GAGTC 2 cut(s) 236, 881
ScrFI CCNGG 2 cut(s) 1059, 1100
SduI GDGCHC 3 cut(s) 72, 405, 795
SfaNI GCATC 3 cut(s) 88, 1115, 1321
SinI GGWCC 3 cut(s) 253, 581, 1211
SmiMI CAYNNNNRTG 2 cut(s) 186, 721
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 6 cut(s) 320, 454, 585, 601, 632, 1169
SsiI CCGC 3 cut(s) 887, 1304, 1340
SspMI CTAG 2 cut(s) 108, 1185
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 1057, 1098
StyI CCWWGG 3 cut(s) 204, 1032, 1335
TaiI ACGT 2 cut(s) 516, 537
TaqI TCGA 6 cut(s) 15, 504, 684, 759, 854, 938
TasI AATT 6 cut(s) 320, 454, 585, 601, 632, 1169
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 3 cut(s) 345, 506, 1255
Tru1I TTAA 4 cut(s) 434, 531, 927, 1266
Tru9I TTAA 4 cut(s) 434, 531, 927, 1266
TseI GCWGC 4 cut(s) 965, 1153, 1223, 1226
TspDTI ATGAA 5 cut(s) 214, 318, 451, 659, 1055
TspGWI ACGGA 1 cut(s) 1041
VpaK11BI GGWCC 3 cut(s) 253, 581, 1211
XapI RAATTY 2 cut(s) 454, 632
XbaI TCTAGA 1 cut(s) 107
XceI RCATGY 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 684
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 2 cut(s) 108, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.