Rh5DG422400

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001754
Physical Location & Seq
Reverse (-)
67351699 .. 67355170
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG422400.1

Sequence Viewer

Length: 1212 bp
ATGGGTACTTTGCAGTCTTTCTACATTGGTGGAGGATATAACCCAGCATCTGTACTTGCAGGATTTGACCAAGAAATACTCGAAAGTGCATTCAATGTTACATCATCAGAACTAAGTGATTTCTTGTCAAGGCAACAAGATGGTCCGATTGTATATGTGACTGATTCACCTCATCCACCAAGCCTTTGGTCAAGATTCCTAGAGCTGAAAGAGCATGACAGACTCCAAAATATGAAGAGAATGGTGGGCTTCACAAAAGAACCTGATCACCAAGAAGAAGACCAATCATTAGCATGGTCATGGAGGAAGCTTCTCAACTCCGTGTTCGGATCAAATGACAAGAGAAGAGACGATCCCGATGCTTACAAGGGAAAAGGAAAATCTCATGATGCTTACAACCTCTTCAAGAGATCTGCAGATTTCAAAAACGACTATGGCTGGAGCAAGGCACTTGACGAATCTGATTACTCGCCACTAAAAGACTCTGGCGTTGGTGTCTTCCTTGTCAACCTTTCTGCAGGGTCAATGATGGCACCACATGTGAACCCAATAGCAACAGAGTATGGGATTGTATTGAGAGGCTCTGGCACAATACAGATTGTATTTCCAAACGGAACCTCGGCAATGAATACTAAAGTTCAAGAAGGAGATGTGTTTTGGGTCCCAAGGTACTTCCCATTCTGTCAAATTGCATCAAGAACTGGTCCTCTTGAGTTCTTTGGATTCACCACCTCGGCACGCAAGAACAGGCCACAGTTCTTGGTTGGTGCTAGCTCTGTTCTTAGGGAGCTCAGAGGACCTGAGCTTGCAGCTGCTTTTGGTGTGAATGAGGATCGGTTGAGTCGCATTGTTCATGCTCAGCGCGAGTCCGTGATCTTGCCTTCGGTGCAAGCAGCTCCTCCTTACAAGGGGGAGGAGAGAGAGTCAACCCCGGAGGAGGAGACAGGGAAGCAAACCCCATACAAGGAGGACGAGAGGAGGCAGGAAGACAGGAAGAGGCGGGAAGATAAGAAGAGGCGGGAAGACGAGAGGGAGCAATCAGCTGAGGAAGATGAGAGAAGGCAACCCCCATACAAGGAAGATGAGAGGCAGCAGTCGTCACAGGAGAAGCAGAGGAGGCAACCTCCATACGGGGAAGGGGATGTGATGTTTCAGAGGCTCCCAAAGGTGATCAAGAGCTTCGGGAATGAGATGGTTATGGGTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

403

Amino Acids

45.99

Weight (kDa)

5.65

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 132 - 277 7.4e-33 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 532
AccII CGCG 1 cut(s) 864
AciI CCGC 2 cut(s) 1000, 1018
AclWI GGATC 3 cut(s) 337, 347, 840
AfaI GTAC 3 cut(s) 7, 54, 671
AfiI CCNNNNNNNGG 5 cut(s) 908, 937, 964, 1075, 1130
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 4 cut(s) 94, 406, 424, 641
AluBI AGCT 9 cut(s) 205, 310, 774, 790, 805, 812, 896, 1043, 1179
AluI AGCT 9 cut(s) 205, 310, 774, 790, 805, 812, 896, 1043, 1179
Alw21I GWGCWC 1 cut(s) 792
Alw26I GTCTC 2 cut(s) 342, 935
AlwI GGATC 3 cut(s) 337, 347, 840
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 1 cut(s) 749
ApeKI GCWGC 4 cut(s) 809, 812, 893, 1090
AspLEI GCGC 1 cut(s) 864
AspS9I GGNCC 4 cut(s) 143, 661, 704, 797
AsuC2I CCSGG 1 cut(s) 932
AsuHPI GGTGA 4 cut(s) 159, 260, 718, 1180
AsuNHI GCTAGC 1 cut(s) 770
AvaII GGWCC 4 cut(s) 143, 661, 704, 797
BanI GGYRCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 4 cut(s) 285, 490, 993, 1029
Bbv12I GWGCWC 1 cut(s) 792
BbvCI CCTCAGC 1 cut(s) 1044
BbvI GCAGC 4 cut(s) 799, 821, 905, 1102
BccI CCATC 3 cut(s) 134, 523, 1186
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BclI TGATCA 2 cut(s) 265, 1170
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 2 cut(s) 342, 935
BfaI CTAG 2 cut(s) 200, 771
BfmI CTRYAG 2 cut(s) 414, 516
BglII AGATCT 1 cut(s) 410
BisI GCNGC 4 cut(s) 810, 813, 894, 1091
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 4 cut(s) 811, 814, 895, 1092
Bme1390I CCNGG 1 cut(s) 932
Bme18I GGWCC 4 cut(s) 143, 661, 704, 797
BmgT120I GGNCC 4 cut(s) 143, 661, 704, 797
BmiI GGNNCC 5 cut(s) 534, 616, 662, 663, 1160
BmrFI CCNGG 1 cut(s) 932
BmsI GCATC 4 cut(s) 56, 349, 379, 701
BmtI GCTAGC 1 cut(s) 774
BpiI GAAGAC 4 cut(s) 285, 490, 993, 1029
BplI GAGNNNNNCTC 2 cut(s) 1108, 1140
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 2 cut(s) 801, 1044
Bpu1102I GCTNAGC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 932
BsaJI CCNNGG 4 cut(s) 618, 665, 732, 930
BsaXI ACNNNNNCTCC 2 cut(s) 908, 938
Bsc4I CCNNNNNNNGG 5 cut(s) 908, 937, 964, 1075, 1130
Bse1I ACTGG 1 cut(s) 706
Bse3DI GCAATG 1 cut(s) 630
BseDI CCNNGG 4 cut(s) 618, 665, 732, 930
BseGI GGATG 2 cut(s) 172, 1147
BseLI CCNNNNNNNGG 5 cut(s) 908, 937, 964, 1075, 1130
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 4 cut(s) 792, 805, 872, 1035
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 6 cut(s) 888, 929, 950, 953, 991, 1129
BseXI GCAGC 4 cut(s) 799, 821, 905, 1102
BseYI CCCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 864
BshFI GGCC 1 cut(s) 751
BshNI GGYRCC 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 792
BsiSI CCGG 1 cut(s) 932
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 5 cut(s) 908, 937, 964, 1075, 1130
BsmAI GTCTC 2 cut(s) 342, 935
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 647
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 1 cut(s) 751
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
Bsp1720I GCTNAGC 1 cut(s) 858
BspACI CCGC 2 cut(s) 1000, 1018
BspANI GGCC 1 cut(s) 751
BspCNI CTCAG 4 cut(s) 793, 804, 871, 1036
BspFNI CGCG 1 cut(s) 864
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 5 cut(s) 534, 616, 662, 663, 1160
BspMAI CTGCAG 2 cut(s) 418, 520
BspOI GCTAGC 1 cut(s) 774
BspPI GGATC 3 cut(s) 337, 347, 840
BspT107I GGYRCC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 4 cut(s) 618, 665, 732, 930
BssMI GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
BssT1I CCWWGG 1 cut(s) 665
Bst4CI ACNGT 1 cut(s) 756
Bst6I CTCTTC 5 cut(s) 230, 340, 407, 989, 1007
BstC8I GCNNGC 4 cut(s) 739, 772, 807, 891
BstDEI CTNAG 6 cut(s) 113, 782, 791, 801, 858, 1044
BstF5I GGATG 2 cut(s) 172, 1147
BstFNI CGCG 1 cut(s) 864
BstHHI GCGC 1 cut(s) 864
BstKTI GATC 7 cut(s) 268, 332, 355, 413, 835, 876, 1173
BstMAI GTCTC 2 cut(s) 342, 935
BstMBI GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
BstMWI GCNNNNNNNGC 3 cut(s) 211, 886, 1117
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 930
BstSFI CTRYAG 2 cut(s) 414, 516
BstUI CGCG 1 cut(s) 864
BstV1I GCAGC 4 cut(s) 799, 821, 905, 1102
BstV2I GAAGAC 4 cut(s) 285, 490, 993, 1029
BstX2I RGATCY 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 186
BstYI RGATCY 1 cut(s) 410
BsuRI GGCC 1 cut(s) 751
BtsCI GGATG 2 cut(s) 172, 1147
Cac8I GCNNGC 4 cut(s) 739, 772, 807, 891
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 864
Cfr13I GGNCC 4 cut(s) 143, 661, 704, 797
Csp6I GTAC 3 cut(s) 6, 53, 670
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 6 cut(s) 215, 294, 300, 386, 539, 854
CviQI GTAC 3 cut(s) 6, 53, 670
DdeI CTNAG 6 cut(s) 113, 782, 791, 801, 858, 1044
DpnI GATC 7 cut(s) 267, 331, 354, 412, 834, 875, 1172
DpnII GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
Eam1104I CTCTTC 5 cut(s) 230, 340, 407, 989, 1007
EarI CTCTTC 5 cut(s) 230, 340, 407, 989, 1007
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 665
Eco24I GRGCYC 1 cut(s) 792
Eco47I GGWCC 4 cut(s) 143, 661, 704, 797
Eco53kI GAGCTC 1 cut(s) 790
EcoICRI GAGCTC 1 cut(s) 790
EcoO109I RGGNCCY 2 cut(s) 661, 797
EcoT14I CCWWGG 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 665
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 6 cut(s) 218, 297, 303, 389, 542, 857
FaqI GGGAC 1 cut(s) 647
FatI CATG 6 cut(s) 214, 293, 299, 385, 538, 853
FauI CCCGC 2 cut(s) 993, 1011
FbaI TGATCA 2 cut(s) 265, 1170
Fnu4HI GCNGC 4 cut(s) 810, 813, 894, 1091
FokI GGATG 2 cut(s) 159, 1154
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 4 cut(s) 810, 813, 894, 1091
FspBI CTAG 2 cut(s) 200, 771
GlaI GCGC 1 cut(s) 863
GluI GCNGC 4 cut(s) 810, 813, 894, 1091
GsaI CCCAGC 1 cut(s) 47
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 1 cut(s) 751
HapII CCGG 1 cut(s) 932
HhaI GCGC 1 cut(s) 864
Hin1II CATG 6 cut(s) 218, 297, 303, 389, 542, 857
Hin6I GCGC 1 cut(s) 862
HinP1I GCGC 1 cut(s) 862
HincII GTYRAC 2 cut(s) 508, 927
HindII GTYRAC 2 cut(s) 508, 927
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 9 cut(s) 164, 195, 222, 458, 482, 723, 841, 866, 923
HpaII CCGG 1 cut(s) 932
HphI GGTGA 4 cut(s) 159, 260, 718, 1180
Hpy166II GTNNAC 3 cut(s) 508, 544, 927
Hpy188I TCNGA 6 cut(s) 109, 147, 329, 463, 794, 1155
Hpy188III TCNNGA 9 cut(s) 192, 356, 386, 406, 641, 696, 710, 1174, 1183
Hpy8I GTNNAC 3 cut(s) 508, 544, 927
HpyAV CCTTC 4 cut(s) 638, 891, 1053, 1130
HpyCH4III ACNGT 1 cut(s) 756
HpyCH4V TGCA 8 cut(s) 13, 59, 89, 416, 518, 692, 809, 889
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 886, 1117
HpyF3I CTNAG 6 cut(s) 113, 782, 791, 801, 858, 1044
Hsp92II CATG 6 cut(s) 218, 297, 303, 389, 542, 857
HspAI GCGC 1 cut(s) 862
KflI GGGWCCC 1 cut(s) 661
Ksp22I TGATCA 2 cut(s) 265, 1170
Kzo9I GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
LmnI GCTCC 5 cut(s) 441, 787, 901, 1033, 1164
Lsp1109I GCAGC 4 cut(s) 799, 821, 905, 1102
LweI GCATC 4 cut(s) 56, 349, 379, 701
MaeI CTAG 2 cut(s) 200, 771
MaeIII GTNAC 3 cut(s) 97, 157, 1098
MalI GATC 7 cut(s) 267, 331, 354, 412, 834, 875, 1172
MboI GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 1 cut(s) 687
MlyI GAGTC 5 cut(s) 216, 476, 850, 875, 932
MslI CAYNNNNRTG 2 cut(s) 292, 298
MspA1I CMGCKG 2 cut(s) 812, 1043
MspI CCGG 1 cut(s) 932
MspR9I CCNGG 1 cut(s) 932
Mva1269I GAATGC 1 cut(s) 89
MvnI CGCG 1 cut(s) 864
MwoI GCNNNNNNNGC 3 cut(s) 211, 886, 1117
NciI CCSGG 1 cut(s) 932
NdeII GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
NheI GCTAGC 1 cut(s) 770
NlaIII CATG 6 cut(s) 218, 297, 303, 389, 542, 857
NlaIV GGNNCC 5 cut(s) 534, 616, 662, 663, 1160
NmeAIII GCCGAG 2 cut(s) 599, 713
NmuCI GTSAC 2 cut(s) 157, 1098
NspI RCATGY 1 cut(s) 542
PagI TCATGA 1 cut(s) 385
PciI ACATGT 1 cut(s) 538
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 4 cut(s) 164, 195, 458, 723
PkrI GCNGC 4 cut(s) 811, 814, 895, 1092
PleI GAGTC 5 cut(s) 216, 476, 849, 874, 931
PpsI GAGTC 5 cut(s) 216, 476, 849, 874, 931
PpuMI RGGWCCY 2 cut(s) 661, 797
PscI ACATGT 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 792
Psp5II RGGWCCY 2 cut(s) 661, 797
PspFI CCCAGC 1 cut(s) 43
PspN4I GGNNCC 5 cut(s) 534, 616, 662, 663, 1160
PspPI GGNCC 4 cut(s) 143, 661, 704, 797
PspPPI RGGWCCY 2 cut(s) 661, 797
PstI CTGCAG 2 cut(s) 418, 520
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 410
PvuII CAGCTG 2 cut(s) 812, 1043
RsaI GTAC 3 cut(s) 7, 54, 671
RsaNI GTAC 3 cut(s) 6, 53, 670
RseI CAYNNNNRTG 2 cut(s) 292, 298
SacI GAGCTC 1 cut(s) 792
SatI GCNGC 4 cut(s) 810, 813, 894, 1091
Sau3AI GATC 7 cut(s) 265, 329, 352, 410, 832, 873, 1170
Sau96I GGNCC 4 cut(s) 143, 661, 704, 797
SchI GAGTC 5 cut(s) 216, 476, 850, 875, 932
ScrFI CCNGG 1 cut(s) 932
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 4 cut(s) 56, 349, 379, 701
SfcI CTRYAG 2 cut(s) 414, 516
SinI GGWCC 4 cut(s) 143, 661, 704, 797
SmiMI CAYNNNNRTG 2 cut(s) 292, 298
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse9I AATT 1 cut(s) 687
SsiI CCGC 2 cut(s) 1000, 1018
SspMI CTAG 2 cut(s) 200, 771
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 1 cut(s) 930
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 1 cut(s) 756
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 687
TatI WGTACW 1 cut(s) 52
TfiI GAWTC 4 cut(s) 164, 195, 458, 723
TseFI GTSAC 2 cut(s) 157, 1098
TseI GCWGC 4 cut(s) 809, 812, 893, 1090
Tsp45I GTSAC 2 cut(s) 157, 1098
TspDTI ATGAA 3 cut(s) 248, 641, 842
TspGWI ACGGA 3 cut(s) 310, 627, 859
VpaK11BI GGWCC 4 cut(s) 143, 661, 704, 797
XceI RCATGY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XspI CTAG 2 cut(s) 200, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.