Rh5AG395400

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001754
Physical Location & Seq
Reverse (-)
68477352 .. 68479484
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG395400.1

Sequence Viewer

Length: 1383 bp
ATGGGAAACAGAGTGATGGTGAGCCTCCTGGTTCTTGTGCTTGTGCTTTCCTATGGAGTTATGGCAATGGCTATGAGGTTTAATCATGAAGATGAAGAGTGGGAAGGGCACCGAGGGCATCACCATCTTTTCCTTTTGCAACAATCAAGGCTCCTGGTGAAAACTGAAGCTGGGCAAATGAGGGTGGTGAGAAGTGTAGGTCAGAGAATGGTAGATAGGCATATAAATGTTGGCTATCTCACCTTGGAACCTAACTCCCTCTTCATCCCACAGTACCTCGACTCCAGCTTGATCCTCTTTGTCCAAAGAGGGCAAGCAAATGTGGGACTGGTACACCGACAGGAATTGGGGGAGAGACAATTGAAGGCTGGGGATGTGTACAGAATTCCAGCAGGTTCCACATTTTACTTGCAGAATGTCAGGGAGAACAGGAGACTTCAGATCATTCTCAGCATCGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCTACCCAAAATCTGTACTTTCTGGGTTCGATTCTGAGATACTCACAAATGCATTTAACGTCTCATCTTCAGAAATAAGGCAGGTTTTGACAAGGCAACAAGAGGGTCCAATTATATATCTAAATAATTCCCAATCATCATCAAACCTTTGGACCAAATTCGTAAGCATGAAAGAGCAGGACAGACTACAAGAATTGAAGAAAATGGTGGACTTCCCAGAAGAACCAGAGCAAACCCTAACTTGGTCATGGAGGAAACTGTTGAACTCCGTGTTCGGAACACTTGAAAATGAGGAGAGGAAGCAACATCATCATGAGCACTCCCAGAAATCCTACAACCTCTACGATAGAAAGCCAGATTTCAAGAACAACCATGGATGGACCCTCTCAGTTGATGAGTCTGATTACTCTCCCCTAAAGGACTCCGGCATTGGCGTTTACCTTGTTAGTCTCAACGCAGGGTCAATGTTGGCACCACATGTGAACCCAAGAGCAACAGAATACGGCGTCGTTCTGAGAGGCTCAGGATCACTACAGATTGTATTTCCAAATGGTACCTTGGCAATGAAGGCGAAACTAAAGCCAGGGAGAGTGTTTTGGGTGCCAAGGTATTTCCCATTTGTCCAAGTGGCATCTAACAACGAGCAACTTGAGATTTTTGGGTTCACTACCTCAGCACAGCCGAACCGGCCACAGTTTTTGGCTGGTTCTAGCTCGGTGCTGCAAGCTCTAAGAGGTCAAGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCATTTTGTTAATGCTCAGCGCGAAGCTGTCATTTTGCCATACAATGCAGAGAGGAATCAAAAGGCAGATGGGATGGCAGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

460

Amino Acids

52.14

Weight (kDa)

8.84

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 46 - 197 3.7e-11 Cupin
Cupin_1 PF00190 283 - 428 3.3e-33 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 383, 586
Acc65I GGTACC 1 cut(s) 1067
AccB1I GGYRCC 4 cut(s) 108, 985, 1067, 1114
AccII CGCG 1 cut(s) 1317
AclWI GGATC 2 cut(s) 286, 1048
AcoI YGGCCR 1 cut(s) 1202
AcsI RAATTY 3 cut(s) 384, 493, 671
AcuI CTGAAG 3 cut(s) 186, 422, 567
AcyI GRCGYC 1 cut(s) 1020
AfaI GTAC 6 cut(s) 275, 333, 380, 484, 531, 1069
AfiI CCNNNNNNNGG 1 cut(s) 756
AflIII ACRYGT 1 cut(s) 991
AgeI ACCGGT 1 cut(s) 1377
AgsI TTSAA 6 cut(s) 364, 712, 778, 800, 877, 1294
AjnI CCWGG 3 cut(s) 27, 153, 1096
AluBI AGCT 6 cut(s) 170, 288, 1227, 1241, 1265, 1322
AluI AGCT 6 cut(s) 170, 288, 1227, 1241, 1265, 1322
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 4 cut(s) 349, 427, 580, 968
AlwI GGATC 2 cut(s) 286, 1048
AoxI GGCC 1 cut(s) 1202
ApeKI GCWGC 3 cut(s) 1234, 1262, 1265
ApoI RAATTY 3 cut(s) 384, 493, 671
AsiGI ACCGGT 1 cut(s) 1377
Asp700I GAANNNNTTC 1 cut(s) 497
Asp718I GGTACC 1 cut(s) 1067
AspLEI GCGC 1 cut(s) 1317
AspS9I GGNCC 3 cut(s) 620, 666, 894
AsuHPI GGTGA 5 cut(s) 31, 113, 169, 199, 232
AvaII GGWCC 3 cut(s) 620, 666, 894
BaeGI GKGCMC 1 cut(s) 111
BanI GGYRCC 4 cut(s) 108, 985, 1067, 1114
Bbv12I GWGCWC 1 cut(s) 834
BbvCI CCTCAGC 1 cut(s) 1186
BbvI GCAGC 3 cut(s) 1221, 1252, 1274
BccI CCATC 5 cut(s) 10, 132, 885, 1358, 1363
BceAI ACGGC 1 cut(s) 1033
BciT130I CCWGG 3 cut(s) 29, 155, 1098
BcoDI GTCTC 4 cut(s) 349, 427, 580, 968
BfaI CTAG 1 cut(s) 1224
BfmI CTRYAG 1 cut(s) 1046
BfuAI ACCTGC 2 cut(s) 383, 586
BglI GCCNNNNNGGC 1 cut(s) 1201
BisI GCNGC 3 cut(s) 1235, 1263, 1266
BlpI GCTNAGC 1 cut(s) 1311
BlsI GCNGC 3 cut(s) 1236, 1264, 1267
Bme1390I CCNGG 3 cut(s) 29, 155, 1098
Bme18I GGWCC 3 cut(s) 620, 666, 894
BmgT120I GGNCC 3 cut(s) 620, 666, 894
BmrFI CCNGG 3 cut(s) 29, 155, 1098
BmsI GCATC 3 cut(s) 127, 462, 1154
BpmI CTGGAG 1 cut(s) 268
Bpu10I CCTNAGC 2 cut(s) 1036, 1186
Bpu1102I GCTNAGC 1 cut(s) 1311
BpuEI CTTGAG 1 cut(s) 1184
BsaHI GRCGYC 1 cut(s) 1020
BsaJI CCNNGG 6 cut(s) 112, 243, 886, 1071, 1097, 1118
BsaWI WCCGGW 1 cut(s) 1377
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 1 cut(s) 756
Bse118I RCCGGY 2 cut(s) 1200, 1377
Bse1I ACTGG 1 cut(s) 333
Bse3DI GCAATG 2 cut(s) 72, 1083
BseBI CCWGG 3 cut(s) 29, 155, 1098
BseDI CCNNGG 6 cut(s) 112, 243, 886, 1071, 1097, 1118
BseGI GGATG 4 cut(s) 264, 379, 896, 1374
BseLI CCNNNNNNNGG 1 cut(s) 756
BseMI GCAATG 2 cut(s) 72, 1083
BseMII CTCAG 7 cut(s) 463, 540, 915, 1019, 1050, 1200, 1325
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 821
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 3 cut(s) 1221, 1252, 1274
BseYI CCCAGC 2 cut(s) 170, 368
Bsh1236I CGCG 1 cut(s) 1317
BshFI GGCC 1 cut(s) 1204
BshNI GGYRCC 4 cut(s) 108, 985, 1067, 1114
BshTI ACCGGT 1 cut(s) 1377
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 3 cut(s) 939, 1201, 1378
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 756
BsmAI GTCTC 4 cut(s) 349, 427, 580, 968
BsmBI CGTCTC 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 1 cut(s) 1204
Bsp1286I GDGCHC 2 cut(s) 111, 834
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 3 cut(s) 291, 441, 1040
Bsp1720I GCTNAGC 1 cut(s) 1311
Bsp19I CCATGG 1 cut(s) 886
BspANI GGCC 1 cut(s) 1204
BspCNI CTCAG 7 cut(s) 462, 541, 914, 1020, 1049, 1199, 1324
BspFNI CGCG 1 cut(s) 1317
BspHI TCATGA 2 cut(s) 85, 826
BspMI ACCTGC 2 cut(s) 383, 586
BspPI GGATC 2 cut(s) 286, 1048
BspT107I GGYRCC 4 cut(s) 108, 985, 1067, 1114
BsrDI GCAATG 2 cut(s) 72, 1083
BsrFI RCCGGY 2 cut(s) 1200, 1377
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 2 cut(s) 1200, 1377
BssECI CCNNGG 6 cut(s) 112, 243, 886, 1071, 1097, 1118
BssMI GATC 3 cut(s) 291, 441, 1040
BssNI GRCGYC 1 cut(s) 1020
BssT1I CCWWGG 4 cut(s) 243, 886, 1071, 1118
Bst2UI CCWGG 3 cut(s) 29, 155, 1098
Bst4CI ACNGT 4 cut(s) 273, 470, 774, 1209
Bst6I CTCTTC 2 cut(s) 90, 266
BstACI GRCGYC 1 cut(s) 1020
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 315, 1239
BstDEI CTNAG 8 cut(s) 449, 549, 901, 1028, 1036, 1186, 1244, 1311
BstDSI CCRYGG 1 cut(s) 886
BstF5I GGATG 4 cut(s) 264, 379, 896, 1374
BstFNI CGCG 1 cut(s) 1317
BstHHI GCGC 1 cut(s) 1317
BstKTI GATC 3 cut(s) 294, 444, 1043
BstMAI GTCTC 4 cut(s) 349, 427, 580, 968
BstMBI GATC 3 cut(s) 291, 441, 1040
BstMWI GCNNNNNNNGC 3 cut(s) 115, 1082, 1201
BstNI CCWGG 3 cut(s) 29, 155, 1098
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 3 cut(s) 27, 153, 1096
BstSFI CTRYAG 1 cut(s) 1046
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 1317
BstV1I GCAGC 3 cut(s) 1221, 1252, 1274
BsuRI GGCC 1 cut(s) 1204
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 4 cut(s) 264, 379, 896, 1374
BveI ACCTGC 2 cut(s) 383, 586
Cac8I GCNNGC 2 cut(s) 315, 1239
CciI TCATGA 2 cut(s) 85, 826
CfoI GCGC 1 cut(s) 1317
Cfr10I RCCGGY 2 cut(s) 1200, 1377
Cfr13I GGNCC 3 cut(s) 620, 666, 894
CseI GACGC 1 cut(s) 1009
Csp6I GTAC 6 cut(s) 274, 332, 379, 483, 530, 1068
CspAI ACCGGT 1 cut(s) 1377
CspCI CAANNNNNGTGG 2 cut(s) 1194, 1229
CviAII CATG 6 cut(s) 86, 682, 762, 827, 887, 992
CviQI GTAC 6 cut(s) 274, 332, 379, 483, 530, 1068
DdeI CTNAG 8 cut(s) 449, 549, 901, 1028, 1036, 1186, 1244, 1311
DpnI GATC 3 cut(s) 293, 443, 1042
DpnII GATC 3 cut(s) 291, 441, 1040
EaeI YGGCCR 1 cut(s) 1202
Eam1104I CTCTTC 2 cut(s) 90, 266
EarI CTCTTC 2 cut(s) 90, 266
Eco130I CCWWGG 4 cut(s) 243, 886, 1071, 1118
Eco47I GGWCC 3 cut(s) 620, 666, 894
Eco57I CTGAAG 3 cut(s) 186, 422, 567
EcoRI GAATTC 1 cut(s) 384
EcoRII CCWGG 3 cut(s) 27, 153, 1096
EcoT14I CCWWGG 4 cut(s) 243, 886, 1071, 1118
EcoT22I ATGCAT 1 cut(s) 568
ErhI CCWWGG 4 cut(s) 243, 886, 1071, 1118
Esp3I CGTCTC 1 cut(s) 580
FaeI CATG 6 cut(s) 89, 685, 765, 830, 890, 995
FaqI GGGAC 1 cut(s) 339
FatI CATG 6 cut(s) 85, 681, 761, 826, 886, 991
Fnu4HI GCNGC 3 cut(s) 1235, 1263, 1266
FokI GGATG 3 cut(s) 251, 386, 903
Fsp4HI GCNGC 3 cut(s) 1235, 1263, 1266
FspBI CTAG 1 cut(s) 1224
GlaI GCGC 1 cut(s) 1316
GluI GCNGC 3 cut(s) 1235, 1263, 1266
GsaI CCCAGC 2 cut(s) 174, 372
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 1 cut(s) 1204
HapII CCGG 3 cut(s) 939, 1201, 1378
HgaI GACGC 1 cut(s) 1009
HhaI GCGC 1 cut(s) 1317
Hin1I GRCGYC 1 cut(s) 1020
Hin1II CATG 6 cut(s) 89, 685, 765, 830, 890, 995
Hin6I GCGC 1 cut(s) 1315
HinP1I GCGC 1 cut(s) 1315
HinfI GANTC 6 cut(s) 281, 545, 911, 935, 1294, 1351
HpaII CCGG 3 cut(s) 939, 1201, 1378
HphI GGTGA 5 cut(s) 31, 113, 169, 199, 232
Hpy166II GTNNAC 6 cut(s) 334, 379, 724, 952, 997, 1179
Hpy188I TCNGA 8 cut(s) 204, 441, 466, 550, 586, 791, 916, 1029
Hpy188III TCNNGA 5 cut(s) 86, 827, 877, 1038, 1253
Hpy8I GTNNAC 6 cut(s) 334, 379, 724, 952, 997, 1179
Hpy99I CGWCG 1 cut(s) 1025
HpyAV CCTTC 4 cut(s) 98, 358, 1075, 1281
HpyCH4III ACNGT 4 cut(s) 273, 470, 774, 1209
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 6 cut(s) 139, 412, 566, 1237, 1262, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 1082, 1201
HpyF3I CTNAG 8 cut(s) 449, 549, 901, 1028, 1036, 1186, 1244, 1311
HpySE526I ACGT 1 cut(s) 573
Hsp92I GRCGYC 1 cut(s) 1020
Hsp92II CATG 6 cut(s) 89, 685, 765, 830, 890, 995
HspAI GCGC 1 cut(s) 1315
KpnI GGTACC 1 cut(s) 1071
Kzo9I GATC 3 cut(s) 291, 441, 1040
LmnI GCTCC 1 cut(s) 156
Lsp1109I GCAGC 3 cut(s) 1221, 1252, 1274
LweI GCATC 3 cut(s) 127, 462, 1154
MaeI CTAG 1 cut(s) 1224
MaeII ACGT 1 cut(s) 573
MalI GATC 3 cut(s) 293, 443, 1042
MboI GATC 3 cut(s) 291, 441, 1040
MboII GAAGA 7 cut(s) 101, 107, 253, 490, 573, 724, 746
MfeI CAATTG 1 cut(s) 359
MhlI GDGCHC 2 cut(s) 111, 834
MluCI AATT 8 cut(s) 344, 359, 384, 493, 624, 640, 671, 707
MlyI GAGTC 3 cut(s) 275, 920, 929
Mph1103I ATGCAT 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 3 cut(s) 81, 570, 1305
MslI CAYNNNNRTG 3 cut(s) 90, 225, 825
MspA1I CMGCKG 1 cut(s) 1265
MspI CCGG 3 cut(s) 939, 1201, 1378
MspR9I CCNGG 3 cut(s) 29, 155, 1098
MunI CAATTG 1 cut(s) 359
MvaI CCWGG 3 cut(s) 29, 155, 1098
MvnI CGCG 1 cut(s) 1317
MwoI GCNNNNNNNGC 3 cut(s) 115, 1082, 1201
NcoI CCATGG 1 cut(s) 886
NdeII GATC 3 cut(s) 291, 441, 1040
NlaIII CATG 6 cut(s) 89, 685, 765, 830, 890, 995
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 1 cut(s) 995
PagI TCATGA 2 cut(s) 85, 826
PciI ACATGT 1 cut(s) 991
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 3 cut(s) 545, 1294, 1351
PinAI ACCGGT 1 cut(s) 1377
PkrI GCNGC 3 cut(s) 1236, 1264, 1267
PleI GAGTC 3 cut(s) 275, 919, 929
PpsI GAGTC 3 cut(s) 275, 919, 929
PscI ACATGT 1 cut(s) 991
Psp6I CCWGG 3 cut(s) 27, 153, 1096
PspFI CCCAGC 2 cut(s) 170, 368
PspGI CCWGG 3 cut(s) 27, 153, 1096
PspPI GGNCC 3 cut(s) 620, 666, 894
PvuII CAGCTG 1 cut(s) 1265
RsaI GTAC 6 cut(s) 275, 333, 380, 484, 531, 1069
RsaNI GTAC 6 cut(s) 274, 332, 379, 483, 530, 1068
RseI CAYNNNNRTG 3 cut(s) 90, 225, 825
SaqAI TTAA 3 cut(s) 81, 570, 1305
SatI GCNGC 3 cut(s) 1235, 1263, 1266
Sau3AI GATC 3 cut(s) 291, 441, 1040
Sau96I GGNCC 3 cut(s) 620, 666, 894
SchI GAGTC 3 cut(s) 275, 920, 929
ScrFI CCNGG 3 cut(s) 29, 155, 1098
SduI GDGCHC 2 cut(s) 111, 834
SfaNI GCATC 3 cut(s) 127, 462, 1154
SfcI CTRYAG 1 cut(s) 1046
SinI GGWCC 3 cut(s) 620, 666, 894
SmiMI CAYNNNNRTG 3 cut(s) 90, 225, 825
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
Sse9I AATT 8 cut(s) 344, 359, 384, 493, 624, 640, 671, 707
SspMI CTAG 1 cut(s) 1224
StyD4I CCNGG 3 cut(s) 27, 153, 1096
StyI CCWWGG 4 cut(s) 243, 886, 1071, 1118
TaaI ACNGT 4 cut(s) 273, 470, 774, 1209
TaiI ACGT 1 cut(s) 576
TaqI TCGA 3 cut(s) 279, 456, 543
TasI AATT 8 cut(s) 344, 359, 384, 493, 624, 640, 671, 707
TatI WGTACW 2 cut(s) 378, 529
TfiI GAWTC 3 cut(s) 545, 1294, 1351
Tru1I TTAA 3 cut(s) 81, 570, 1305
Tru9I TTAA 3 cut(s) 81, 570, 1305
TseI GCWGC 3 cut(s) 1234, 1262, 1265
TspDTI ATGAA 6 cut(s) 102, 108, 253, 490, 698, 1094
TspGWI ACGGA 1 cut(s) 772
VpaK11BI GGWCC 3 cut(s) 620, 666, 894
XapI RAATTY 3 cut(s) 384, 493, 671
XceI RCATGY 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 1224
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.