FvH4_3g33240

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001754
Physical Location & Seq
Reverse (-)
28636138 .. 28637947
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33240.t1

Sequence Viewer

Length: 1404 bp
ATGGGAGACAGAGTGATGGTAAGCCTCCTGGCTCTTGTGCTTGTGCTTTCCTATGGAGTTATGGCAATGGCTATGAGCTTTAATCATGAAGATGAAGAATGGGGAAAGGATCAGAGAGAAGAAGAGTGGGAAGAGCATCATCATCATCATCATCACAGAGGGCATCACCATTATTTCCTTCTGCAACGATCAAGGGTCCTGGTGAAAACTGAAGCTGGGGAAATGAGGGTGGTGAGAAGTGTAGGTCATGAAATAGTTGATAGGCATATAAATGTTGGGTTTCTCACTTTGGAACCTAACTCCCTCTTCATCCCTCAGTACCTGGACTCCAGCTTGATCCTCTTTGTCCAAAGAGGGCAAGGAAACCTGGGACTGGTACACCATCATGAACTGGGAGAGAGACAATTGAAGGCTGGGGATGTGCACAGGATCCCAGCAGGTTCCACATTCTACTTGCATAATGCTAGGGAGCACCAGAGACTTCAGATCATTCTCAGCGTTGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCAACCCGAAATCTGTACTTTCTGGGTTTGATTCGGAAATACTCACAAATGCATTTAATGTCTCATCTTCAGAATTAAGGCAGGTTTTGACAAGGCACCAAGAGGGTGCAATTGTACATCTAAAGAATTCTCAATCATCATCATCAAACCTTTGGAGTAAGTTCGTAAGCATGAAAGAGCAGGACAGACTACAAGAGTTGAAGAAATTGGTGGACCTCTCAGAAGAACCGGAGAAAACCCTAACTCGGTCATGGAGGAAACTGTTGAACTCCGTGTTTGGAAAACCTGAAAACGAGAAGAGAAAGCAGCATCATCATGACCACTCACAGGAATCCCACAACCTCTACAATAGAAAGCCAGATTTCAAGAACCACCATGGATGGATCCTCTCAGTTGATGAGTCTAATTTCTCTCCCCTACAAAACTCTGGCGTCGGCGTTCACCTTATTAGACTCAATGCAGGGTCAATGTTGGCACCACATGTGAACCCAGCAGCAACCGAATACGGCGTCGTTACAAGAGGCTCAGGACATCTCCAGATTGTGTTTCCAAATGGAACTTTGGCAATGAAGGCAGACCTAAAGCCAGGGAGTGTGTTTTGGGTGCCGAGGTACTTCCCATTTGTCCAAGTAGCTTCTAACAACGAGCAACTTGAGATTTTTGGGTTCACTACCTCGCCGCAGCCGAACCATCCACAGTTCTTGGCTGGTTCTAGCTCGGTGCTGCAGGCTCTAAGAGGTCAAGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCGCTTTGTCGATGCTCAACGTGAAGCTGTCATTTTGCCCTACAATGCAGAGAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

468

Amino Acids

52.9

Weight (kDa)

6.81

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 64 - 211 4.2e-10 Cupin
Cupin_1 PF00190 299 - 444 1.9e-31 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 428, 631
AccB1I GGYRCC 3 cut(s) 654, 1033, 1162
AciI CCGC 1 cut(s) 1238
AclWI GGATC 6 cut(s) 117, 331, 424, 437, 937, 950
AcsI RAATTY 2 cut(s) 538, 685
AcuI CTGAAG 3 cut(s) 231, 467, 612
AcyI GRCGYC 2 cut(s) 990, 1068
AfaI GTAC 6 cut(s) 320, 378, 529, 576, 675, 1172
AfiI CCNNNNNNNGG 3 cut(s) 373, 804, 886
AflIII ACRYGT 1 cut(s) 1039
AgsI TTSAA 5 cut(s) 409, 760, 826, 925, 1342
AjnI CCWGG 5 cut(s) 27, 198, 321, 366, 1144
AluBI AGCT 7 cut(s) 78, 215, 333, 1193, 1275, 1313, 1370
AluI AGCT 7 cut(s) 78, 215, 333, 1193, 1275, 1313, 1370
Alw21I GWGCWC 2 cut(s) 426, 474
Alw26I GTCTC 3 cut(s) 394, 472, 625
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 6 cut(s) 117, 331, 424, 437, 937, 950
AlwNI CAGNNNCTG 1 cut(s) 322
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 6 cut(s) 865, 1052, 1240, 1282, 1310, 1313
ApoI RAATTY 2 cut(s) 538, 685
ArsI GACNNNNNNTTYG 2 cut(s) 1053, 1085
Asp700I GAANNNNTTC 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 196, 772
AsuHPI GGTGA 4 cut(s) 158, 214, 244, 992
AvaII GGWCC 2 cut(s) 196, 772
BaeGI GKGCMC 1 cut(s) 426
BamHI GGATCC 2 cut(s) 429, 942
BanI GGYRCC 3 cut(s) 654, 1033, 1162
Bbv12I GWGCWC 2 cut(s) 426, 474
BbvI GCAGC 6 cut(s) 877, 1064, 1252, 1269, 1300, 1322
BccI CCATC 4 cut(s) 10, 390, 933, 1257
BceAI ACGGC 1 cut(s) 1081
BciT130I CCWGG 5 cut(s) 29, 200, 323, 368, 1146
BcoDI GTCTC 3 cut(s) 394, 472, 625
BfaI CTAG 2 cut(s) 465, 1272
BfmI CTRYAG 1 cut(s) 1283
BfuAI ACCTGC 2 cut(s) 428, 631
BisI GCNGC 7 cut(s) 866, 1053, 1238, 1241, 1283, 1311, 1314
BlsI GCNGC 7 cut(s) 867, 1054, 1239, 1242, 1284, 1312, 1315
Bme1390I CCNGG 5 cut(s) 29, 200, 323, 368, 1146
Bme18I GGWCC 2 cut(s) 196, 772
BmgT120I GGNCC 2 cut(s) 196, 772
BmiI GGNNCC 9 cut(s) 197, 294, 431, 442, 559, 656, 944, 1035, 1164
BmrFI CCNGG 5 cut(s) 29, 200, 323, 368, 1146
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 4 cut(s) 145, 172, 877, 1345
BmuI ACTGGG 1 cut(s) 401
BpmI CTGGAG 2 cut(s) 313, 1079
Bpu10I CCTNAGC 1 cut(s) 1084
BpuEI CTTGAG 1 cut(s) 1232
BsaHI GRCGYC 2 cut(s) 990, 1068
BsaJI CCNNGG 4 cut(s) 367, 934, 1145, 1166
BsaWI WCCGGW 1 cut(s) 787
BsaXI ACNNNNNCTCC 3 cut(s) 27, 311, 341
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 804, 886
Bse1I ACTGG 2 cut(s) 378, 396
Bse3DI GCAATG 2 cut(s) 72, 1131
BseBI CCWGG 5 cut(s) 29, 200, 323, 368, 1146
BseDI CCNNGG 4 cut(s) 367, 934, 1145, 1166
BseGI GGATG 4 cut(s) 309, 424, 944, 1249
BseLI CCNNNNNNNGG 3 cut(s) 373, 804, 886
BseMI GCAATG 2 cut(s) 72, 1131
BseMII CTCAG 5 cut(s) 329, 508, 792, 963, 1098
BseNI ACTGG 2 cut(s) 378, 396
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 6 cut(s) 877, 1064, 1252, 1269, 1300, 1322
BseYI CCCAGC 4 cut(s) 215, 413, 433, 1048
BshNI GGYRCC 3 cut(s) 654, 1033, 1162
BsiHKAI GWGCWC 2 cut(s) 426, 474
BsiSI CCGG 1 cut(s) 788
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 3 cut(s) 373, 804, 886
BsmAI GTCTC 3 cut(s) 394, 472, 625
BsmFI GGGAC 1 cut(s) 384
Bsp1286I GDGCHC 2 cut(s) 426, 474
Bsp1407I TGTACA 1 cut(s) 673
Bsp143I GATC 6 cut(s) 109, 188, 336, 429, 486, 942
Bsp19I CCATGG 1 cut(s) 934
BspACI CCGC 1 cut(s) 1238
BspCNI CTCAG 5 cut(s) 328, 507, 791, 962, 1097
BspHI TCATGA 4 cut(s) 85, 247, 385, 874
BspLI GGNNCC 9 cut(s) 197, 294, 431, 442, 559, 656, 944, 1035, 1164
BspMAI CTGCAG 1 cut(s) 1287
BspMI ACCTGC 2 cut(s) 428, 631
BspPI GGATC 6 cut(s) 117, 331, 424, 437, 937, 950
BspQI GCTCTTC 1 cut(s) 126
BspT107I GGYRCC 3 cut(s) 654, 1033, 1162
BsrDI GCAATG 2 cut(s) 72, 1131
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 2 cut(s) 378, 396
BssECI CCNNGG 4 cut(s) 367, 934, 1145, 1166
BssMI GATC 6 cut(s) 109, 188, 336, 429, 486, 942
BssNI GRCGYC 2 cut(s) 990, 1068
BssT1I CCWWGG 1 cut(s) 934
Bst2UI CCWGG 5 cut(s) 29, 200, 323, 368, 1146
Bst4CI ACNGT 3 cut(s) 515, 822, 1257
Bst6I CTCTTC 4 cut(s) 117, 126, 311, 851
BstACI GRCGYC 2 cut(s) 990, 1068
BstAUI TGTACA 1 cut(s) 673
BstC8I GCNNGC 1 cut(s) 1287
BstDEI CTNAG 6 cut(s) 315, 494, 778, 949, 1084, 1292
BstDSI CCRYGG 1 cut(s) 934
BstF5I GGATG 4 cut(s) 309, 424, 944, 1249
BstKTI GATC 6 cut(s) 112, 191, 339, 432, 489, 945
BstMAI GTCTC 3 cut(s) 394, 472, 625
BstMBI GATC 6 cut(s) 109, 188, 336, 429, 486, 942
BstMWI GCNNNNNNNGC 1 cut(s) 1130
BstNI CCWGG 5 cut(s) 29, 200, 323, 368, 1146
BstNSI RCATGY 1 cut(s) 1043
BstSCI CCNGG 5 cut(s) 27, 198, 321, 366, 1144
BstSFI CTRYAG 1 cut(s) 1283
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 6 cut(s) 877, 1064, 1252, 1269, 1300, 1322
BstX2I RGATCY 2 cut(s) 429, 942
BstYI RGATCY 2 cut(s) 429, 942
BtgI CCRYGG 1 cut(s) 934
BtsCI GGATG 4 cut(s) 309, 424, 944, 1249
BveI ACCTGC 2 cut(s) 428, 631
Cac8I GCNNGC 1 cut(s) 1287
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 4 cut(s) 85, 247, 385, 874
Cfr13I GGNCC 2 cut(s) 196, 772
CseI GACGC 2 cut(s) 979, 1057
Csp6I GTAC 6 cut(s) 319, 377, 528, 575, 674, 1171
CspCI CAANNNNNGTGG 2 cut(s) 1242, 1277
CviAII CATG 8 cut(s) 86, 248, 386, 730, 810, 875, 935, 1040
CviQI GTAC 6 cut(s) 319, 377, 528, 575, 674, 1171
DdeI CTNAG 6 cut(s) 315, 494, 778, 949, 1084, 1292
DpnI GATC 6 cut(s) 111, 190, 338, 431, 488, 944
DpnII GATC 6 cut(s) 109, 188, 336, 429, 486, 942
Eam1104I CTCTTC 4 cut(s) 117, 126, 311, 851
EarI CTCTTC 4 cut(s) 117, 126, 311, 851
Eco130I CCWWGG 1 cut(s) 934
Eco47I GGWCC 2 cut(s) 196, 772
Eco57I CTGAAG 3 cut(s) 231, 467, 612
EcoO109I RGGNCCY 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 5 cut(s) 27, 198, 321, 366, 1144
EcoT14I CCWWGG 1 cut(s) 934
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 934
FaeI CATG 8 cut(s) 89, 251, 389, 733, 813, 878, 938, 1043
FaqI GGGAC 1 cut(s) 384
FatI CATG 8 cut(s) 85, 247, 385, 729, 809, 874, 934, 1039
Fnu4HI GCNGC 7 cut(s) 866, 1053, 1238, 1241, 1283, 1311, 1314
FokI GGATG 4 cut(s) 296, 431, 951, 1236
Fsp4HI GCNGC 7 cut(s) 866, 1053, 1238, 1241, 1283, 1311, 1314
FspBI CTAG 2 cut(s) 465, 1272
GluI GCNGC 7 cut(s) 866, 1053, 1238, 1241, 1283, 1311, 1314
GsaI CCCAGC 4 cut(s) 219, 417, 437, 1052
GsuI CTGGAG 2 cut(s) 313, 1079
HapII CCGG 1 cut(s) 788
HgaI GACGC 2 cut(s) 979, 1057
Hin1I GRCGYC 2 cut(s) 990, 1068
Hin1II CATG 8 cut(s) 89, 251, 389, 733, 813, 878, 938, 1043
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 6 cut(s) 326, 590, 890, 959, 1011, 1342
HpaII CCGG 1 cut(s) 788
HphI GGTGA 4 cut(s) 158, 214, 244, 992
Hpy166II GTNNAC 7 cut(s) 379, 424, 502, 772, 1000, 1045, 1227
Hpy188I TCNGA 6 cut(s) 114, 486, 511, 595, 631, 781
Hpy188III TCNNGA 8 cut(s) 86, 248, 386, 875, 925, 1086, 1096, 1301
Hpy8I GTNNAC 7 cut(s) 379, 424, 502, 772, 1000, 1045, 1227
Hpy99I CGWCG 2 cut(s) 995, 1073
HpyAV CCTTC 4 cut(s) 188, 403, 1123, 1329
HpyCH4III ACNGT 3 cut(s) 515, 822, 1257
HpyCH4IV ACGT 1 cut(s) 1363
HpyCH4V TGCA 9 cut(s) 184, 424, 457, 611, 668, 1019, 1285, 1310, 1391
HpyF10VI GCNNNNNNNGC 1 cut(s) 1130
HpyF3I CTNAG 6 cut(s) 315, 494, 778, 949, 1084, 1292
HpySE526I ACGT 1 cut(s) 1363
Hsp92I GRCGYC 2 cut(s) 990, 1068
Hsp92II CATG 8 cut(s) 89, 251, 389, 733, 813, 878, 938, 1043
Kzo9I GATC 6 cut(s) 109, 188, 336, 429, 486, 942
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 6 cut(s) 877, 1064, 1252, 1269, 1300, 1322
LweI GCATC 4 cut(s) 145, 172, 877, 1345
MaeI CTAG 2 cut(s) 465, 1272
MaeII ACGT 1 cut(s) 1363
MaeIII GTNAC 1 cut(s) 1072
MalI GATC 6 cut(s) 111, 190, 338, 431, 488, 944
MboI GATC 6 cut(s) 109, 188, 336, 429, 486, 942
MfeI CAATTG 2 cut(s) 404, 669
MflI RGATCY 2 cut(s) 429, 942
MhlI GDGCHC 2 cut(s) 426, 474
MluCI AATT 7 cut(s) 404, 538, 632, 669, 685, 764, 964
MlyI GAGTC 3 cut(s) 320, 968, 1005
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 4 cut(s) 81, 615, 635, 1402
MslI CAYNNNNRTG 4 cut(s) 90, 270, 384, 873
MspA1I CMGCKG 1 cut(s) 1313
MspI CCGG 1 cut(s) 788
MspR9I CCNGG 5 cut(s) 29, 200, 323, 368, 1146
MunI CAATTG 2 cut(s) 404, 669
MvaI CCWGG 5 cut(s) 29, 200, 323, 368, 1146
MwoI GCNNNNNNNGC 1 cut(s) 1130
NcoI CCATGG 1 cut(s) 934
NdeII GATC 6 cut(s) 109, 188, 336, 429, 486, 942
NlaIII CATG 8 cut(s) 89, 251, 389, 733, 813, 878, 938, 1043
NlaIV GGNNCC 9 cut(s) 197, 294, 431, 442, 559, 656, 944, 1035, 1164
NmeAIII GCCGAG 1 cut(s) 1191
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 1 cut(s) 1043
PagI TCATGA 4 cut(s) 85, 247, 385, 874
PciI ACATGT 1 cut(s) 1039
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 1241
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 3 cut(s) 590, 890, 1342
PkrI GCNGC 7 cut(s) 867, 1054, 1239, 1242, 1284, 1312, 1315
PleI GAGTC 3 cut(s) 320, 967, 1005
PpsI GAGTC 3 cut(s) 320, 967, 1005
PpuMI RGGWCCY 1 cut(s) 196
PscI ACATGT 1 cut(s) 1039
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 5 cut(s) 27, 198, 321, 366, 1144
PspFI CCCAGC 4 cut(s) 215, 413, 433, 1048
PspGI CCWGG 5 cut(s) 27, 198, 321, 366, 1144
PspN4I GGNNCC 9 cut(s) 197, 294, 431, 442, 559, 656, 944, 1035, 1164
PspPI GGNCC 2 cut(s) 196, 772
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 1 cut(s) 1287
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 2 cut(s) 429, 942
PvuII CAGCTG 1 cut(s) 1313
RsaI GTAC 6 cut(s) 320, 378, 529, 576, 675, 1172
RsaNI GTAC 6 cut(s) 319, 377, 528, 575, 674, 1171
RseI CAYNNNNRTG 4 cut(s) 90, 270, 384, 873
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 4 cut(s) 81, 615, 635, 1402
SatI GCNGC 7 cut(s) 866, 1053, 1238, 1241, 1283, 1311, 1314
Sau3AI GATC 6 cut(s) 109, 188, 336, 429, 486, 942
Sau96I GGNCC 2 cut(s) 196, 772
SchI GAGTC 3 cut(s) 320, 968, 1005
ScrFI CCNGG 5 cut(s) 29, 200, 323, 368, 1146
SduI GDGCHC 2 cut(s) 426, 474
SfaNI GCATC 4 cut(s) 145, 172, 877, 1345
SfcI CTRYAG 1 cut(s) 1283
SinI GGWCC 2 cut(s) 196, 772
SmiMI CAYNNNNRTG 4 cut(s) 90, 270, 384, 873
SmlI CTYRAG 1 cut(s) 1211
SmoI CTYRAG 1 cut(s) 1211
Sse9I AATT 7 cut(s) 404, 538, 632, 669, 685, 764, 964
SsiI CCGC 1 cut(s) 1238
SspMI CTAG 2 cut(s) 465, 1272
StyD4I CCNGG 5 cut(s) 27, 198, 321, 366, 1144
StyI CCWWGG 1 cut(s) 934
TaaI ACNGT 3 cut(s) 515, 822, 1257
TaiI ACGT 1 cut(s) 1366
TaqI TCGA 1 cut(s) 1353
TaqII GACCGA 1 cut(s) 795
TasI AATT 7 cut(s) 404, 538, 632, 669, 685, 764, 964
TatI WGTACW 2 cut(s) 574, 673
TauI GCSGC 1 cut(s) 1240
TfiI GAWTC 3 cut(s) 590, 890, 1342
Tru1I TTAA 4 cut(s) 81, 615, 635, 1402
Tru9I TTAA 4 cut(s) 81, 615, 635, 1402
TseI GCWGC 6 cut(s) 865, 1052, 1240, 1282, 1310, 1313
TspDTI ATGAA 8 cut(s) 102, 108, 264, 298, 402, 535, 746, 1142
TspGWI ACGGA 1 cut(s) 820
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 196, 772
XapI RAATTY 2 cut(s) 538, 685
XceI RCATGY 1 cut(s) 1043
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 2 cut(s) 465, 1272
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.